Owens José Barros-Barrios’s scientific contributions

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Publications (2)


Sperm Quality, Milt Biochemistry, and Cellular Ultrastructure in Fresh and Cryopreserved Sperm of Brycon amazonicus
  • Article

April 2025

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5 Reads

Owens José Barros-Barrios

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Víctor Mauricio Medina-Robles

Milt cryopreservation facilitates native fish’s constant production of commercial interest. However, it can damage spermatozoa (sptz) due to the osmotic stress of diluents and low temperatures. This study compares spermatic quality in fresh (SF) and cryopreserved (CS) milt in B. amazonicus for 24 h, 1 month, and 3 months. Eight sexually mature males were induced with carp hypophysis extract. Milt was diluted in one proportion in a glucose solution (5.5%), egg yolk (12%), and dimethyl sulfoxide (10%). An evaluation of parameters such as motility, viability, morphology, total antioxidant capacity (TAC), and adenosine triphosphate (ATP) contents was performed at 24 h, 1 month, and 3 months, and the fertility test was conducted at 24 months of cryopreservation. The motility percentage was higher ( p ≤ 0.05) in SF (95.5 ± 2.9%). Motility duration of SF was significantly higher (113.6 ± 22.6 s) than that of CS at 3 months (71 ± 12.2 s). The spermatic viability of SF was higher (97.1 ± 1%) in comparison with CS treatments. Sperm anomalies in SF were lower (12.4 ± 3.2%) than in all other treatments. ATP contents of (1.74 ± 4.5 nM × 10 ⁸ sptz) were superior only ( p ≤ 0.05) in CS of 1 month (1.16 ± 1.2 nM × 10 ⁸ sptz). TAC was higher ( p ≤ 0.05) in SF (26.46 ± 1.2 mM of Trolox [6‐hydroxy‐2,5,7,8‐tetramethilcromane‐acid 2‐carboxylic]) compared to other CS treatments. However, there were not any significant differences in this parameter between cryopreservation times ( p ≤ 0.05). Fertility rates were the same for all treatments. In terms of cellular ultrastructure, it was observed that B. amazonicus sptz had an oval‐shaped head without an acrosome, middle part, and only one flagellum.


Parámetros de calidad espermática en semen crioconservado de peces dulceacuícolas
  • Article
  • Full-text available

December 2022

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26 Reads

Revista Orinoquia

La acuicultura a nivel mundial cada vez toma más relevancia y se ha convertido en una fuente de alimento e ingresos económicos de muchas comunidades alrededor del mundo. Sin embargo, actividades como la sobreexplotación del recurso hídrico y pesquero, el aumento de la población humana, la contaminación ambiental, el cambio climático, entre otros factores, están poniendo en riesgo no solo la seguridad alimentaria del planeta, sino también la biodiversidad de organismos, entre ellos los peces. Una de las herramientas para salvaguardar los recursos biológicos acuáticos, es la crioconservación, que en peces se enfoca principalmente en la conservación de semen en nitrógeno líquido y esta, pesar de ser una técnica muy útil, es susceptible a mejoramiento, ya que se ha reportado que las bajas temperaturas usadas en la crioconservación pueden causar efectos negativos en las células espermáticas, debido a esto es necesario establecer protocolos especie específicos para sacarle todo el potencial a esta forma de conservación. Por lo tanto, el objetivo de esta revisión es recopilar información sobre los principales parámetros de calidad espermática que se ven afectados en el momento de crioconservar el semen, haciendo énfasis en las especies dulceacuícolas.

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