Osman Öztürk’s research while affiliated with Bozok University and other places

Objective With the digitalization of objects and spaces, healthcare services are being reshaped globally, creating many potential applications. This study aimed to determine the application potential of remote healthcare services (RHS) in a hospital by considering the experiences, interests, and suggestions of health professionals, and examples of useful applications that can be used, developed, or invented for healthcare systems. Methods A semi-structured, face-to-face interview survey was conducted with 176 healthcare professionals working at Bozok University. Results Branches with the highest practice experience were internal medicine, cardiac, pediatric, infection, and orthopedics. Experienced participants rated the usability of “Consultation,” “Support,” and “Monitoring” applications higher than other apps, and indicated that they would prefer to use them for themselves (η²≤0.12). Requirements adequacy was lower for older adults, internal/surgical branches, and physicians than for other groups (η² ≥ 0.05). Application categories showed a significant relationship (0.4 ≤ r ≤ 0.8, p < 0.05). Several variables significantly explained the models (p < 0.001): application-usability (64%), user-demand (64%), and requirements-adequacy (25%). Professionals’ demand (r = 0.83) was more strongly correlated to usability than patient demand (r = 0.63). Health professionals (N = 105) from 17 branches provided 57 available, 51 developable, and 19 innovative application recommendations. These were coded according to application type, critical features, presence, integration status, and usefulness. Conclusion RHS’ application potential in a hospital was revealed considering demographic factors and application categories based on health professionals’ experiences, practical interests, and suggestions, with a strong, comprehensive, and up-to-date methodology. The findings have the potential for international application and can contribute to implementing useful and developing original applications.

Tartrazine finds widespread application in the realms of alimentation, pharmaceuticals, cosmetic formulations, and textile manufacturing. Tartrazine has a negative effect on human health such as hyperactivity, allergies and asthma in children. Substances such as tartrazine might effect the embryo in a kind of aspects, containing physical or mental disorders, and a decrease in the child's intellectual memory. In this study, Sprague dawley female rats, 70–100 days old, weighing 250–300 g, with confirmed pregnancy, were divided into two groups of 5: control and tartrazine group. Rats were sacrificed on the 20th day and heart, lung, kidney and liver tissues were removed from the fetuses. The effect of tartrazine on fetal bone development was assessed by double skeletal staining, histological analysis on organs, and IL-6, IL-1β, TNF-α, and TRPM2 gene levels. It has been observed that tartrazine, which is frequently used as a food dye, damages important fetal tissues such as liver, kidney, lung and heart. A statistically meaningful reduce was observed in the total length, total area, bone length and bone area values of the limb bones in the tartrazine group compared to the control group (p < 0.05). It was observed that the mRNA levels of IL-6, IL-1β, TNF-α and TRPM2 genes in the livers of the fetuses changed compared to the control group (p < 0.05). In this study on the use of tartrazine during pregnancy, it was observed that both organs and bone development were damaged. More studies are needed on the effects of tartrazine.

Alcohol use disorder has negative effects on the reproductive system through the development of oxidative stress and its genotoxic effects on DNA integrity. Acute and chronic alcohol use adversely affects the male reproductive system. Phosphatidylinositol-3-kinase (PI3K) and mammalian target of rapamycin (mTOR) levels, which are involved in oxidative stress, may be important in the reproductive system in alcohol use disorder. PI3K and mTOR immunoreactivities were evaluated in testicular tissue in an experimental acute and chronic alcohol intake model in male rats. Rats were divided into 3 groups as control male (n=7), acute male (n=7) and chronic male model (n=7). Histopathological analysis of testicular tissues taken from the experimental groups was performed by hematoxylin-eosin (H&E) staining. Then, testicular tissues of the experimental groups were dissected and PI3K and mTOR expressions were determined by immunohistochemistry method. According to the H&E staining results, when the experimental groups were compared with the control group; It was observed that spermatozoa were less or absent in acute and chronic groups. mTOR and PI3K expressions were significantly increased in testicular tissues belonging to chronic and acute alcohol model groups. In the chronic alcohol model, both mTOR and PI3K expressions were significantly increased compared to the acute and control groups. Our research reveal that PI3K and mTOR molecules, which are involved in oxidative stress in acute and chronic alcohol intake, may be associated with damage to the reproductive system. PI3K and mTOR proteins, can be targeted at the point of treatment against alcohol-induced reproductive damage.

Tartrazine, an azo dye prevalent in pharmaceuticals and food items, was investigated for its impact on fetal development, specifically examining visceral and skeletal abnormalities in rat offspring exposed to daily oral doses throughout pregnancy. Fourteen pregnant rats were randomly assigned to control and tartrazine groups (seven animals each), with tartrazine administered via oral gavage at 7.5 mg/kg throughout gestation. Offspring were categorized by gender for histopathological and genetic analysis of visceral structures. Bone quality and fracture resistance assessments involved micro‐CT, Raman spectroscopy, and biomechanical testing. Results highlighted distinct internal organ tissue differences in the tartrazine group, notably increased hemorrhagic and inflammatory cell infiltration, degeneration, and vacuolization compared to controls. Gender‐specific alterations in mRNA levels of IL‐6, IL‐1β, TNF‐α, and TRPM2 genes (p < .001) were also noted. Moreover, tartrazine‐exposed groups exhibited reduced trabecular thickness, bone volume, and significant alterations in bone matrix composition and quality alongside significant decreases in fracture resistance (p < 0.05). This study concludes that intrauterine exposure to tartrazine can result in adverse impacts on organ and bone development in rat offspring.

Amaç: Bu çalışmada, gıdalarda yaygın olarak kullanılan Tartrazinin, önemli sitokinlerden olan ve tümör büyümesi ile yakından ilişkili olan IL-6’nın ve hücre farklılaşması, apoptoz ile otofajide önemli rol oynayan Beklin-1 proteininin rat plasenta dokusundaki ekspresyon yoğunluğu araştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu çalışmada, 10 adet 70-100 günlük, 250-300 gr ağırlığındaki, Sprague Dawley ırkı dişi ratlar erkek ratlarla çiftleştirilerek dişi ratların plasentaları kullanıldı. Kontrol grubu (n=5) ve Tartrazin grubu (n=5) deney hayvanları gebeliklerinin 20. gününde sakrifiye edilerek plasentaları alındı. Hematoksilen-Eozin boyamasıyla histopatolojik değerlendirmesi yapıldı ve IL-6 ile Beklin-1 ekspresyonunu analiz etmek için immünohistokimyasal olarak histolojik incelemeleri yapıldı. Bulgular: Plasentaların histopatolojisine bakıldığında, Tartrazin grubunun kontrol grubuna kıyasla, plasenta dokularında morfolojik olarak major bir değişim görülmedi. İmmünohistokimyasal olarak, Tartrazin grubu plasenta dokusunda Labrint alanda ve glikojenik hücrelerde Beklin-1 proteininin yoğun eksprese olduğu, kontrol grubuyla kıyaslandığında ekspresyon şiddetinin istatistiksel olarak aralarında anlamlı bir farkın olduğu görüldü (p