Nurlaili Nurlaili’s research while affiliated with Syiah Kuala University and other places

Lutjanus kasmira is one of the snappers occupying and commonly found in the Arabian Sea and the Bengal Strait. The purpose of this study was to examine the comparative phylogeography pattern of Lutjanus kasmira in the Bengal Strait and the Arabian Sea using the mitochondrial COI gene as a marker. The population sampled of Indonesia was taken from fish landing in Banda Aceh, Indonesia, while the population of India represented Bengal Strait, Oman, Yemen, Djibouti and Somalia respresented Arabian sea was retrieved from the NCBI Genbank. The extraction method was carried out using a modified C-TAB protocol. In this study at total of 47 sequences were analysed that produced 22 haplotypes where 18 haplotypes distributed in the Arabian Sea and 4 haplotypes from Bengal Strait. 10 samples of Oman population produced 6 haplotypes, 10 samples from Djibouti produced 5 haplotypes, 5 haplotypes from the population of Yemen, Somalia 2 haplotypes and India 1 haplotypes, respectively. Meanwhile, the Indonesian population, from 6 samples, produced 3 haplotypes. L. kasmira population from Arabian Sea and Bengal strait had no connectivity. The highest haplotype diversity (Hd) value was found in Oman population of 0.956 and the lowest found in Somalia population, while Indonesia population has a moderate genetic diversity.

BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre- freezing temperatures of 4°C, −10°C, and −79°C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at −179°C for 7 days. RESULTS: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (ρ<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.

The principle of sperm preservation is to prolong the viability of spermatozoa by reducing the degree of metabolism during storage at low temperatures, and one method involved was a cryoprotectant as a sperm protector. This study aimed to determine the best type of cryoprotectant for refrigeration storage of goldfish Carrasius auratus sperm. A completely randomized design was used, and five types of cryoprotectant were tested, namely Methanol, Ethanol, Ethylene Glycol, Glycerol, and DMSO at a 10% concentration combined with 15% egg yolks. The sperm was diluted with Ringer’s solution and stored at 4⁰C for 48 hours. The ANOVA test revealed that cryoprotectant significantly affected the motility and viability of sperm after 48 hours of refrigeration (P0.05). The results showed that 10% DMSO combined with 15% egg yolk could maintain sperm motility and viability better than other treatments, with values of 85.80% and 86.50%, respectively, which were significantly different from the other treatments. Glycerol produced the second-best sperm quality with motility and viability values of 71.13% and 74.13%, respectively. Therefore, DMSO is the most effective cryoprotectant for refrigeration storage of goldfish sperm.