Nurit Argov-Argaman's research while affiliated with Hebrew University of Jerusalem and other places

Publications (45)

Article
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Milk lipids are organized in the form of milk fat globules (MFG), ranging in size from 0. 1 to 15 μm. The MFG size is closely associated with the composition of fatty acids, polar lipids, sphingolipids, cholesterol and the content of the MFG membrane (MFGM). Also, the MFGM integral proteins and glycoconjugates differ in composition and structure be...
Article
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Primary culture of mammary epithelial cells (MEC) was exposed to ethyl-acetate, chloroform and hexane extracts of Pistacia lentiscus (lentisk). The hexane extract contained mainly ethyl gallate whereas the chloroform extract contained mainly ethyl-gallate with smaller amount of gallic acid, and the ethyl-acetate extract contained mainly rutin, gall...
Article
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An association between progressive motility (PM) and spermatozoa fertility competence has been suggested. However, the mechanism that underlies PM is not clear enough. We examined physiological characteristics and fatty acid composition of fresh spermatozoa with high and low PM. Additional analysis of fatty acid composition and structural character...
Article
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We assessed the potential of Pistacia lentiscus (lentisk) phenolic compounds to enhance production of milk composition in lactating goats and caprine primary mammary epithelial cells (MEC). Damascus goats were given a lentisk infusion (LI) or fresh water (FW) to drink, in a crossover design. Milk from LI vs. FW goats was 0.43% richer in fat and 30%...
Article
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We assessed the potential of phenolic compounds from Pistacia lentiscus (lentisk) to enhance production of milk constituents in bovine mammary epithelial cells (MEC). MEC were exposed to 0 (control), 1 or 10 ppm of polyphenols from lentisk ethanolic extract (PLEE) for 24 h. PLEE were absorbed by the MEC plasma membrane, but also penetrated the cell...
Article
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N-3 polyunsaturated fatty acids (PUFAs) are essential nutrients that must be obtained from the diet. We have previously showed that endogenous n-3 PUFAs contribute to skeletal development and bone quality in fat-1 mice. Unlike other mammals, these transgenic mice, carry the n-3 desaturase gene and thus can convert n-6 to n-3 PUFAs endogenously. Sin...
Article
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Progesterone plays a pivotal role during mammogenesis and serves as an inhibitor of the secretory activation of mammary cells in the last days of gestation. However, its role during lactogenesis, in particular its involvement in lipid metabolism, and milk fat content and composition, is unknown. Here, we provide new evidence of progesterone's invol...
Article
We evaluated the effects of processing - pasteurization and yoghurt manufacturing - on some health-promoting lipidome components in milk from two feeding treatments – brushland grazing or hay-feeding in confinement - in dairy goats. The contents of fat and protein were higher, and of urea, lower, in grazing goats. Fatty acid composition - at the ex...
Article
We have previously shown that grazing in East-Mediterranean brushlands is associated with improved milk quality. However, grazing exposes animals to predation, heat stress, and parasites, and imposes labor constraint. In order to verify the hypothesis that feeding the tannin-rich browse species lentisk (Pistacia lentiscus L.) to confined dairy goat...
Article
Atrazine (ATZ) is one of the most extensively used herbicides to control growth of broadleaf and grassy weeds in crops. ATZ and its metabolites have deleterious effect on sperm quality. ATZ is also known for its ability to induce oxidative stress. Pistacia lentiscus (PL) is an evergreen shrub, with a high content of polyphenols in leaf extracts, wi...
Article
Milk fat globule (MFG) size ranges over 3 orders of magnitude, from less than 200 nm to over 15 µm. The significance of MFG size derives from its tight association with its lipidome and proteome. More specifically, small MFG have relatively higher content of membrane compared with large globules, and this membrane exerts diverse positive health eff...
Chapter
The evolution of lactation and the composition, structures, and functions of milk's biopolymers illustrates that the Darwinian pressure on lactation selected for gene products with considerable structural complexity and diverse functions within the digestive system. For example, complex sugar polymers - oligosaccharides - possess unique properties...
Data
Data for all the result are presented as mean ±SE ans SEM. (XLSX)
Article
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The effects of the macrostructure of long chain fatty acids on the lipid metabolism and biosynthesis of lipid droplets (LD) was studied in mammary epithelial cells (MEC). MEC were exposed to similar compositions and concentrations of fatty acids in the form of either triglycerides (Tg), as part of the very-low-density lipids (VLDL) isolated from la...
Data
Mammary epithelial cells (MEC) treated with 100 μM VLDL or similar concentration and composition of for 24 h. (A) Lipoprotein lipase (LPL) mRNA expression (AU). (B) NADH dehydrogenase (ubiquinone) 1α subcomplex assembly factor 3 (NDUFAF3) mRNA expression (AU) (C) Peroxisome proliferator-activated receptor gamma (PPAR-γ) mRNA expression (AU). (TIF)
Data
Mammary epithelial cells (MEC) treated with 100 μM VLDL or similar concentration and composition of for 24 h. Fatty Acid Binding Protein (FABP) mRNA expression (AU). (TIF)
Article
Full-text available
Mammary epithelial cells (MEC) secrete fat in the form of milk fat globules (MFG) which are found in milk in diverse sizes. MFG originate from intracellular lipid droplets, and the mechanism underlying their size regulation is still elusive. Two main mechanisms have been suggested to control lipid droplet size. The first is a well-documented pathwa...
Article
Objective A pilot study to determine lipoprotein classes and subclasses in premature infants and examine associations with nutritional intake, gestational age (GA), and morbidity. Study Design Plasma lipoprotein particle concentrations were analyzed in a cohort of 15 premature infants in the first 5 days of life and again at 2 weeks. Breast milk sa...
Article
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Non-alcoholic fatty liver disease (NAFLD) represents a spectrum of pathologies, ranging from hepatocellular steatosis to non-alcoholic steatohepatitis (NASH), fibrosis and cirrhosis. It has been suggested that fish oil containing n-3 polyunsaturated fatty acids (n-3 PUFA) induce beneficial effects in NAFLD. However, n-3 PUFA are sensitive to peroxi...
Article
Background: Breastfeeding is beneficial for maternal-infant dyads affected by gestational diabetes, although women with gestational diabetes often have lower breastfeeding rates and face more challenges with early breastfeeding than nondiabetic women. Metabolite concentration shifts of lactose and citrate indicate the transition to secretory activa...
Article
The interactive effect of breed and feeding management on milk composition was established in local goats (Damascus, Mamber) and their F1 Alpine crossbreeds, half of which grazed daily for 4 h in Mediterranean brushland (Pasture – P) and half were fed clover hay (Hay – H) indoors, in addition to concentrate fed individually. Milk composition and fa...
Article
Objective: A primary study objective was to examine differences in fatty acid concentrations in colostrum of women with and without GDM. The effect of gestational diabetes mellitus (GDM) on fatty acid composition of colostrum is not fully understood, although rates of GDM are increasing globally. Research Methods & Procedures: A prospective case-c...
Article
In bovine milk, fat globules (MFG) have a heterogeneous size distribution with diameters ranging from 0.1 to 15 µm. Although efforts have been made to explain differences in lipid composition, little is known about the protein composition of MFG membranes (MFGM) in different sizes of MFG. In this study, protein and lipid analyses were combined to s...
Article
Human colostrums and transition milk was collected from women under the age of 37 years and women aged 37 years and older. Transition milk of the younger group had lower fat content and 10 fold higher concentrations of omega 6 FA, eicosadecanoic and arachdonic acids. Gestational age affected the colostrum concentration of total fat and omega 3 and...
Article
Milk fat globule (MFG) size and phospholipids (PL) content and composition were determined in milk collected at 65 (pretreatment), 110, 135 and 170 days of lactation from goats randomly assigned to grazing in Mediterranean brushland or fed clover hay indoors, in addition to concentrate. Daily feed intake and dietary contents of neutral detergent fi...
Article
Objectives: To test the null hypothesis that mothers of asymmetric small for gestational age (SGA) infants produce milk with fatty acids composition similar to that of lactating mothers of appropriate-for-gestational-age (AGA) infants. Methods: We obtained human milk (HM) from 2 groups of lactating volunteers that gave birth to asymmetric SGA (stud...
Article
Full-text available
Milk fat globule size is determined by the size of its precursors-intracellular lipid droplets-and is tightly associated with its composition. We examined the relationship between phospholipid composition of mammary epithelial cells and the size of both intracellular and secreted milk fat globules. Primary culture of mammary epithelial cells was cu...
Article
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We studied the effects of the frequently used glucogenic dietary supplementation in dairy herds and the hormonal changes occurring during the normal estrous cycle on the composition and concentration of milk lipid components. Holstein dairy cows were synchronized with two injections of prostaglandin F2 α (estrus=day 0). Animals were held as control...
Article
The mechanism underlying the shift in milk-fat-globule (MFG) mean diameter upon changing the concentrate-to-forage ratio in dairy cow rations was investigated. Cows were fed high-concentrate low-forage (HCLF) or high-forage low-concentrate (LCHF) rations for 4weeks. Mean diameter of MFG, determined in raw whole milk, was 0.4μm larger in the LCHF-fe...
Article
We examined the effects of concentrate-to-forage ratio in dairy cow rations on milk-fat composition, with a specific focus on the structure of milk fat globules (MFG). Twenty-four Holstein cows, 153 d in milk, were assigned to 2 dietary treatments in a crossover design study. Treatments were (1) high-concentrate (65%), low-forage (35%; HCLF) diet a...
Article
Full-text available
During secretion of milk fat globules, triacylglycerol (TAG) droplets are enveloped by a phospholipid (PL) trilayer. Globule size has been found to be related to polar lipid composition and fat content, and milk fat content and fatty acid composition have been associated with the diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism; however,...
Article
Season-induced variation in fatty acid and cholesterol composition in bovine semen has been associated with semen quality. Given the specific roles of the various semen compartments (seminal fluids, sperm head, and sperm tail) in fertilization, we hypothesized that environmental-stress-induced alterations in the lipid composition of a specific comp...
Article
The aim was to determine the relative role of each of the lactogenic hormones (insulin, prolactin and hydrocortisol) and their combinations in regulating elongation and desaturation of polyunsaturated fatty acids and subsequently on composition of cellular lipid compartments in mammary epithelia. Cultured cells of the mammary gland epithelial cell...
Article
We determined the effect of insulin on milk fatty acid (FA) and lipid composition in goats. Four dairy goats, 150d in milk, were subjected to hyperinsulinemic clamp (treatment) or saline (control) infusion for 4d in a crossover design study. Composition and concentration of plasma and milk FA, triglycerides, phospholipids, sphingolipids, and choles...
Article
Full-text available
Ruminal wall metabolism was studied in nonlactating heifers by altering the carbohydrate (CHO) digestion site between rumen and intestine. The CHO digestion site was estimated from in situ and total-tract digestibility of control (CONT) diets and diets supplemented with corn (CRN), barley (BARL), or soy hulls (SOYH). Ruminal epithelial metabolism r...
Article
Full-text available
We examined the association between plasma insulin concentration and fatty acid distribution to milk fat and membrane fractions (triglyceride and phospholipid, respectively) in dairy cows. Thirty-seven Holstein cows at 60 to 70 d postpartum were synchronized by 2 PGF(2α) injections 14 d apart. Plasma samples were taken before morning and evening mi...
Article
The mammary epithelial cell produces unique structures and a range of diversely sized lipid particles from tens of micrometers to less than 1 μm. The physical, chemical, and biological properties of the differently sized milk fat globules (MFGs) and their complex membranes are not well described. Six size fractions of MFGs were obtained by gravity-...
Article
Milk fat globules (MFGs) are accepted primarily as triacylglycerol delivery systems. The identification of nanometer-sized lipid-protein particles termed "lactosomes" that do not contain triacylglycerol raises the question of their possible functions. MFGs were isolated by slow centrifugation, and lactosomes were isolated by ultracentrifugation at...

Citations

... Lipid peroxidation leads to disturbances in membrane fluidity and permeability, causing a reduction of motility and a decrease in the sperm ability to interact and fuse with the oocyte [23]. Even though the injury may affect membranes of any cell type, those of sperm are known to be especially susceptible to oxidative damage due to their great content of polyunsaturated fatty acids [24]. In addition, thiol oxidation by ROS affects the activity of some enzymes, and may also lead to alterations on sperm chromatin, as protamines are rich in thiol groups (cysteine residues) [18,25]. ...
... Force-displacement data were collected by the Instron software BlueHill (version 2.0, Instron Corporation, Norwood, MA, USA) at 10 Hz. The resulting force-displacement curves were used to calculate bone stiffness, bone yield point, load of fracture, maximal load, and the area under the curve was measured to calculate the total energy to fracture (E to F) [36]. Moreover, a stress-strain curve was calculated in order to determine the stiffness of the material excluding bone geometry, the Young's modulus [37]. ...
... An in-depth investigation of the mechanism underlying the greater production was conducted in a primary culture of mammary gland epithelial cells (MEC) treated with lentisk extract. Treated cells had increased energy production, and cellular resources were preferably channeled to the production of milk constituents (Hadaya et al., 2020b). ...
... Cervical epithelial cells mirror breast epithelial tissue. Amongst the key drivers for poor prognostic breast cancer 17,20 , progesterone is well known for its lipogenic effect in adipocytes but also breast cells [33][34][35] . Hence we wanted to assess whether aberrant DNAme at progesterone receptor binding sites (PR-BS) 36,37 in atrisk breast tissue is mirrored in cervical cells. ...
... 134 Similarly, higher PL levels were observed in goat milk yoghurt versus goat milk, where the source material was obtained from confined (i.e., not grazing) animals. 135 Some LAB can also metabolise PLs. ...
... The beneficial effect of dietary phenolic compounds on production traits has been demonstrated. For example, lactating goats browsed on phenolic compounds-rich pasture produced milk with higher fat and especially omega 3 content, higher protein and lower urea content compared to their counterparts fed a poor-phenolic compounds diet (Hadaya et al., 2017;Hadaya et al., 2020a). Dietary composition revealed that these goats consumed mainly lentisk (Pistacia lentiscus), a phenolic compounds-rich (20% dry matter [DM] basis) evergreen brush species (Landau, Muklada, Markovics, & Azaizeh, 2014). ...
... Pomegranate peel is the source of phenolic compounds like catechin, epicatechin, lignins, ellagic tannin, etc. [155]. The important constituents of pomegranate peel are carbon, oxygen and hydrogen, nitrogen along with the functional groups like carboxylic acid, phenol, carbonyl, etc. ...
... In an effort to study the effect of dietary phenolic compounds on production, two approaches are commonly used: (i) in vivowhere phenolic compounds are ingested as part of the diet (Komsky-Elbaz et al., 2019;Shabtay et al., 2012). While this approach allow to study the overall effect on production, it does not distinguish between phenolic compounds activity in the gastrointestinal tract (GIT) and their systemic post-absorption effects on the milk producing cells; (ii) in vitrodirect administration of phenolic compounds extract to cells (Hadaya et al., 2020b). ...
... Fat contributes around half of the energy intake of breastfed infants, and also provides indispensable polyunsaturated fatty acids (PUFAs) and lipid-soluble vitamins [2,5]; it is the most variable macronutrient in human milk, with content ranging from 2.4 to 5.5 g/100 mL (10th to 90th percentiles) [6]. In human milk, lipids are emulsified as milk fat globules (MFGs) that consist of a triacylglycerol (TAG)-rich core surrounded by a tri-layered milk fat globule membrane (MFGM) comprising glycerophospholipids, sphingolipids, cholesterol, and proteins [5,7,8]. Previously the importance of milk LCPUFAs-including n-6 arachidonic acid (ARA) and n-3 docosahexaenoic acid (DHA)-for infant development has been recognized [9], but only recently has the importance of the complex polar lipids in the MFGM for infants' gut maturation and the establishment of the intestinal immune system been described [10]. ...
... Fat in human milk exists in the form of fat globules, with an average diameter of about 3-5 µm. Fat globules are composed of an inner core of triglycerides and milk fat globule membrane (MFGM) [2]. In addition, human milk fat contains a variety of PUFAs, phospholipids, fat-soluble vitamins, and other essential nutrients, which are beneficial to the growth and development of infants. ...