Ning Shi’s research while affiliated with China Agricultural University and other places

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Publications (17)


Effect of rootstocks on grapevine growth potential and rhizosphere microbial diversity. Different letters within the plot indicate significant differences among varieties (Duncan’s multiple range test at p < 0.05). a the dry weight of under-ground and above-ground biomass showed a significant effect of rootstocks on the grapevine growth. B Unconstrained PCoA with Bray–Curtis distance showed that the rhizosphere microbiomes of different treatments were segregated. c, d, α diversity of the rhizosphere bacterial (c) and fungal (d) communities
Effect of rootstocks on the phylum level distribution of rhizosphere microbiome. a, b, Phylum level distribution of rhizosphere bacterial (a) and fungal (b) communities
The differential microbes between CK and rootstock combinations. a Manhattan plot showed the OTUs enriched and depleted in five rootstock combinations. Each circle and triangle represented an OTU. The filled or empty triangles represented OTUs enriched or depleted in this rootstock combination, respectively (FDR adjusted p < 0.05, Wilcoxon rank sum test). OTUs were classified sorted and colored at the phylum level. CPM, counts per million. b the distribution of all the enriched OTUs under the influence of rootstocks. c the distribution of all the depleted OTUs under the influence of rootstocks
Effect of rootstocks on growth potential, soil properties and rhizosphere microbiome of Vitis vinifera L. cv. Cabernet Sauvignon
  • Article
  • Publisher preview available

March 2025

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18 Reads

Plant and Soil

Ning Shi

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Hao-Cheng Lu

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Jun Wang

Aims Rootstocks are widely used in viticulture. Understanding the impact of rootstocks on the structure and function of grapevine rhizosphere microbiota is essential for manipulating them toward the sustainable development of vineyard. Methods We investigated the changes in biomass, soil properties, and rhizosphere microbiome after Vitis vinifera L. cv. Cabernet Sauvignon (CS) grafted onto five rootstock cultivars [Kober 5BB (5BB), Richer 110 (110R), Beta, Riparia Gloire (RG) and Millardet et de Grasset 101–14 (101–14)]. The grapevines were exhumed for biomass measurements and rhizosphere soils were collected for testing the microbiome and soil properties. The rhizosphere microbiome was analysed using the 16S rRNA gene and ITS amplicon sequencing, and the functional annotations were performed using FAPROTAX and FUNGuild database. Results Rootstocks could selectively recruit microbial communities of specific structures, especially fungi. Rootstocks significantly affected the richness and diversity of the fungal community, while significantly altering the composition of the fungal community at the phylum level. The microbial communities associated with the different rootstocks further formed the different function profiles. The grapevine biomass and the soil minerals in the same vineyard were significantly associated with the root genotypes. Conclusions This study highlighted the influence of rootstocks on grapevine and soil environment. The results of this study will provide useful information for vineyard rootstock selection when the microbiological environment of the vineyard needs to be considered. At the same time, new insights into the interactions between the environment, microbiome and grapevine growth were provided.

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Vineyard microclimate alterations induced by black inter-row mulch through transcriptome reshaped the flavoromics of cabernet sauvignon grapes

April 2024

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80 Reads

BMC Plant Biology

Background Weed control is essential for agricultural floor management in vineyards and the inter-row mulching is an eco-friendly practice to inhibit weed growth via filtering out photosynthetically active radiation. Besides weed suppression, inter-row mulching can influence grapevine growth and the accumulation of metabolites in grape berries. However, the complex interaction of multiple factors in the field challenges the understanding of molecular mechanisms on the regulated metabolites. In the current study, black geotextile inter-row mulch (M) was applied for two vintages (2016–2017) from anthesis to harvest. Metabolomics and transcriptomics analysis were conducted in two vintages, aiming to provide insights into metabolic and molecular responses of Cabernet Sauvignon grapes to M in a semi-arid climate. Results Upregulation of genes related to photosynthesis and heat shock proteins confirmed that M weakened the total light exposure and grapes suffered heat stress, resulting in lower sugar-acid ratio at harvest. Key genes responsible for enhancements in phenylalanine, glutamine, ornithine, arginine, and C6 alcohol concentrations, and the downward trend in ε-viniferin, anthocyanins, flavonols, terpenes, and norisoprenoids in M grapes were identified. In addition, several modules significantly correlated with the metabolic biomarkers through weighted correlation network analysis, and the potential key transcription factors regulating the above metabolites including VviGATA11, VviHSFA6B, and VviWRKY03 were also identified. Conclusion This study provides a valuable overview of metabolic and transcriptomic responses of M grapes in semi-arid climates, which could facilitate understanding the complex regulatory network of metabolites in response to microclimate changes.



Fig. 1. (a) Wine color charts generated based on CIELab parameters. (b) Marselan pomace and Petit Manseng pomace obtained after a press cycle. (c) Effect of adjusting the pomace ratio on the concentration of phenolic compounds in Marselan wines. Different lowercase letters above the column indicate significant differences in the same compounds (Duncan's multiple range test at p < 0.05). Cy, cyanidin; Dp, delphinidin; Pe, peonidin; Pt, petunidin; Mv, malvidin; F-e-A, flavanolethyl-anthocyanin adducts; F-PA, flavanyl-pyranoanthocyanins; H-PA, hydroxyphenyl-pyranoanthocyanins; La, laricitrin-based flavonols; My, myricetin-based flavonols; Ka, kaempferol-based flavonols; Qu, quercetin-based flavonols; Is, isorhamnetin-based flavonols; Sy, syringetin-based flavonols; Di, dihydroquercetin and dihydrokeampferol; C, catechin; EC, epicatechin; EGC, epigallocatechin; P-B1, procyanidin B1; P-B2, procyanidin B2. For individual compounds, the concentrations are located in Supplementary Table 3.
Fig. 2. Effect of adjusting the pomace ratio on the concentration of volatile compounds in Marselan wines. Different lowercase letters above the column indicate significant differences in the same compounds (Duncan's multiple range test at p < 0.05).
Fig. 3. OPLS-DA based on volatile compound concentrations in Marselan wines with different treatments and control. The 54 volatile compounds with VIP values > 1.0 were marked with labels, and others were hidden.
Effect of adjusting the pomace ratio on the oenological and chromatic parame- ters of Marselan wines.
Adjusting the pomace ratio during red wine fermentation: Effects of adding white grape pomace and juice runoff on wine flavoromics and sensory

October 2023

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100 Reads

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9 Citations

Food Chemistry X

Adding pomace or juice runoff during maceration is a traditional winemaking process. To mitigate the negative effects of rainfall during harvest and examine the effects of adjusting the pomace ratio during fermentation on the flavor profile of Marselan grape wines, the prefermentation addition of Petit Manseng grape pomace (PAP) and prefermentation juice runoff (PJR) was determined. The phenolic and volatile compounds were investigated using HPLC–MS and GC–MS. PAP enriched the flavanols and PJR enriched the pigment and copigment matrix. Approximate 10% increase in the ratio of pomace promoted the formation of anthocyanin derivatives. The increased pomace ratio reduced the concentration of volatile compounds without impacting the aroma quality. Sensory analysis revealed PAP wines scored higher for acidity and astringency and PJR wines scored higher for color. In conclusion, an appropriate increase in the pomace ratio of approximately 10% can enhance the color and mouthfeel of the wine while having a limited influence on aroma.


Citations (10)


... The a* value is inversely correlated with total anthocyanins. Notably, a* is positively correlated with Dp, Mv, Pn-ac, Mv-ac, and tMv-cou, suggesting that these monomeric anthocyanins may significantly contribute to the red hue of grapes and wines Li et al., 2024). The b* value is negatively correlated with Cy, Pt, Pn, Mv, Pn-ac, and tMvcou, indicating that these anthocyanins impart a blue tint to the wine. ...

Reference:

Anthocyanin profiles and color parameters of fourteen grapes and wines from the eastern foot of Helan Mountain in Ningxia
Volatomic differences among Vitis amurensis cultivars and its hybrids with V. vinifera revealed the effects of genotype, region, and vintage on grape aroma
  • Citing Article
  • July 2024

... The volatile concentrations were quantified using internal standard and standard curve methods, while those of compounds without standard curves were calculated according to the standard curve of compounds with the same functional group and/or similar numbers of carbon atoms, expressed as μg/L fruit juice or wine. In addition, the odor activity value (OAV) of the wine volatiles was calculated according to the odor reference threshold described in previous studies (Kong et al., 2019;Lu et al., 2024;Wang et al., 2017;Yao, Jin, et al., 2021). Volatiles with OAV > 1 were considered to have made significant contributions to the typical aroma traits of the wine samples and are listed in Supplementary Table S1. ...

Volatilomics of Cabernet Sauvignon grapes and sensory perception of wines are affected by canopy side in vineyards with different row orientations
  • Citing Article
  • July 2024

Food Chemistry

... In an earlier study, Liu et al. analyzed the volatile compounds in cabernet Sauvignon grape berries and detected 55 kinds of volatile compounds, among which ketones, alcohols, aldehydes, and a small amount of ester compounds were mainly detected [8]. Shi et al. used HPLC-QqQ-MS/MS and GC-MS to detect the volatile compounds of five grape varieties, among which the volatile compounds mainly included hexal, (E)-2-hexenal, (E, Z) -2, 6-nonadienal, β-hemalone, and (E))-2-nonenal gives the grape a more floral, fruity, and earthy flavor [26]. The results of this study show that there are significant differences in berry aromas between the 182 germplasm, which also means that some varieties have special aromas due to the high genetic abundance of grapes. ...

Metabolomics of Vitis davidii Foëx. grapes from southern China: Flavonoids and volatiles reveal the flavor profiles of five spine grape varieties
  • Citing Article
  • May 2024

Food Chemistry

... According to Gancel et al. (2022), malic acid is described as a "green" and "aggressive" acid, with high acidifying power, and its perception threshold is higher than that of tartaric acid, which may justify the lower acceptance of juices with higher malic acid concentration. In the production of red wines, aiming to achieve better sensory acceptance of the beverage, malolactic fermentation is generally carried out to convert the malic acid into lactic acid (Tian et al., 2024). ...

The pH adjustment of Vitis amurensis dry red wine revealed the evolution of organic acids, volatomics, and sensory quality during winemaking
  • Citing Article
  • October 2023

Food Chemistry

... Similar significant sensory differences were observed in Premium-quality Cabernet Sauvignon wines due to mannoprotein-producing yeasts ( Table 6). In general, all treatments presented similar patterns, and the Y5 yeast could be distinguished in terms of parameters such as fat, velvety astringency, smoothness, and roundness, suggesting a positive impact on texture, balance, and overall quality [54]. These variations underscore the multifaceted role of yeast, which influences fermentation dynamics and contributes significantly to the perceived qualities of the final Premium wine product [55]. ...

Vineyard soil heterogeneity and harvest date affect volatolomics and sensory attributes of Cabernet Sauvignon wines on a meso-terroir scale
  • Citing Article
  • September 2023

Food Research International

... El atributo de tonalidad violácea permitió diferenciar a los vinos producidos en la región. Dicho atributo está relacionado con el color del vino, el cual es influenciado por diversos factores, entre los que destacan la variedad de uva (Kharadze et al., 2018), las condiciones geográficas y climáticas (Lu et al., 2023), así como el proceso de producción (Liu et al., 2018). Estos factores influyen en el contenido de antocianinas de los vinos, que son la principal fuente del color (Jackson, 2020). ...

The key role of vineyard parcel in shaping flavonoid profiles and color characteristics of Cabernet Sauvignon wines combined with the influence of harvest ripeness, vintage and bottle aging

Food Chemistry X

... For instance, grafting has been shown to enhance K uptake in tomatoes and cucumbers [10,11], leading to better growth and yield. Several studies have demonstrated that rootstock grafting can alter the expression of regulatory metabolic pathways or specific genes in scions [12,13]. Additionally, epigenetic mechanisms also contribute to the plant's response to grafting [14]. ...

Modifications of Vitis vinifera L. cv. Petit Verdot grape flavonoids as affected by the different rootstocks in eastern China

Technology in Horticulture

... In this work, we also expected to find differences in the topping and bud pruning between conventional and organic farming. Since a severe topping could alter vine growth (Lu et al., 2023), adjusting topping could contribute to limit vine sensitivity to pest and diseases, even though this lowered sensitivity would not be enough to control disease development. But we did not find any changes in topping and bud pruning during conversion to organic viticulture. ...

Severe shoot topping slows down berry sugar accumulation rate, alters the vine growth and photosynthetic capacity, and influences the flavoromics of Cabernet Sauvignon grapes in a semi-arid region
  • Citing Article
  • April 2023

European Journal of Agronomy

... The additional manifestation may be explained by the fact that distinct categories of variants in the SOX10 gene may display various clinical features by affecting different signaling pathways in WS. It has been reported that SOX10 gene variants may result in pubertal delay [8,9]. One case was identified as co-existence of WS2 and Kallmann syndrome associated with SOX10 gene variants [10]. ...

Diagnosis and genetic analysis of a case of Waardenburg syndrome type 2 with hypogonadotropic hypogonadism caused by SOX10 gene deletion
  • Citing Article
  • December 2022

Hereditas (Beijing)

... The soil indicators were determined regarding Han et al. (2023), as follows: A soil/KCl solution ratio of 1:2.5 (w:v) was used to determine the soil pH; organic matter (OM) was measured using sulfochromic oxidation; electrical conductivity (EC) was determined using a conductivity meter; N in soil were determined using the Kjeldahl method; other elements (P, K, Ca, Mg) were determined using an ICP-MS (Agilent 7800, USA), as described in Han et al. (2023), and the detailed steps were as follows: A soil sample was weighed 0.25 g and added to 5 ml of HNO 3 for digestion, then the solution was heated at 100 °C for 30 min. After the solution cooled, 1 mL of H 2 O 2 was added and heated using a dry thermostat at 40 °C until nearly dry. ...

Region, vintage, and grape maturity co-shaped the ionomic signatures of the Cabernet Sauvignon wines
  • Citing Article
  • November 2022

Food Research International