Nina Otberg's research while affiliated with Moses-Mendelssohn-Zentrum Potsdam, Germany and other places

Publications (69)

Article
Scalp imaging techniques are necessary tools for the trichological practice and for visualization of permeation, penetration and absorption processes into and through the scalp and for the research on drug delivery and toxicology. The present letter reviews different scalp imaging techniques and discusses their utility. Moreover, two different stud...
Article
Primary cicatricial alopecias refer to a group of rare, idiopathic, inflammatory scalp disorders that result in permanent hair loss. Primary cicatricial alopecias comprise a diverse group of inflammatory diseases and can be classified via different approaches, such as clinical presentation, histopathologic findings, or both. Primary cicatricial alo...
Article
The aim of this study was to define the underlying relative penetration of caffeine through hair follicles and through intact stratum corneum with time in vivo through pharmacokinetic modelling. Caffeine plasma concentration-time profiles after topical application into skin with or without hair follicle blocking were modelled using the Wagner-Nelso...
Article
Of the world population, 1.7% is suffering from alopecia areata at some point in their lives. The exact etiology of this disease is still unknown, and the course of the disease is unpredictable. Effective treatments, especially for severe multifocal alopecia areata, alopecia areata totalis, and alopecia areata universalis, are lacking. The present...
Article
Our modern society faces an increasing life-expectation, and along with this, an increased desire for a youthful appearance. Hair plays a major role in our overall appearance, our self-conception and self-esteem. The experience of hair loss is distressing at any age and can reveal our aging process. Aging hair becomes more fragile, is thinner and s...
Article
Uncombable hair syndrome is a relatively rare anomaly of the hair shaft, with less than 100 cases reported to date, that results in a disorganized, unruly hair pattern that is impossible to comb flat. The characteristic longitudinal grooves along the hair shaft, along with the triangular or kidney-shaped cross section allows this condition to be di...
Article
Due to ethical reasons, in vivo penetration studies are not applicable at all stages of development of new substances. Therefore, the development of appropriate in vitro methods is essential, as well as the comparison of the obtained in vivo and in vitro data, in order to identify their transferability. The aim of the present study was to investiga...
Article
Liposomes in the nanosize range have been recognized as a versatile drug delivery system of both hydrophilic and lipophilic molecules. In order to develop a liposome-based topical vaccination strategy, five different types of liposomes were tested as a putative vaccine delivery system on pig ear skin. The investigated liposomes mainly varied in siz...
Article
Various nonfollicular scalp conditions can cause secondary scarring or permanent alopecia. Possible causes are congenital defects, trauma, inflammatory conditions, infections, and neoplasms (rarely drugs). Associated signs and symptoms and other diagnostic procedures such as histopathology may aid in the diagnosis. Detection of the underlying disor...
Article
Folliculitis decalvans is a rare inflammatory scalp disorder. The present paper gives a practical approach to diagnosis and patient management and reviews possible pathogenetic factors and treatment options. Folliculitis decalvans is classified as primary neutrophilic cicatricial alopecia and predominantly occurs in middle-aged adults. Staphylococc...
Article
Lichen planopilaris is a chronic scarring alopecia characterized by follicular hyperkeratosis, perifollicular erythema, and loss of follicular orifices. The scalp lesions may be single or multiple and commonly involve the vertex and parietal area. The hair follicles at the margin of the alopecic patches reveal perifollicular erythema. Anagen hairs...
Article
Pseudopelade of Brocq (PPB) is a rare, idiopathic, slowly progressive hair disorder, resulting in cicatricial alopecia. It typically presents in Caucasian adult patients as small, smooth, flesh-toned and slightly depressed alopecic patches with irregular outlines. It primarily involves the parietal and vertex portions of the scalp with a chronic pr...
Article
What is already known about this subject: * In recent years, it has been suggested that hair follicles represent important shunt routes into the skin for drugs and chemicals [1-3]. * In vitro studies have shown the importance of skin appendages for skin penetration by hydrophilic compounds [4]. Investigation of follicular penetration in vivo has b...
Article
The second part of this 2-part article reviews clinical features, histology, management, and treatment of neutrophilic primary cicatricial alopecias (folliculitis decalvans and dissecting folliculitis) and mixed primary cicatricial alopecias (acne keloidalis, acne necrotica, and erosive pustular dermatosis).
Article
Full-text available
Water-filtered infrared-A (wIRA) irradiation has been shown to enhance penetration of clinically used topically applied substances in humans through investigation of functional effects of penetrated substances like vasoconstriction by cortisone. Investigation of the influence of wIRA irradiation on the dermatopharmacokinetics of topically applied s...
Article
Excisional biopsies and routine histology remains the gold standard for the histomorphologic evaluation of normal and diseased skin. However, there is increasing interest in the development of noninvasive optical technologies for evaluation, diagnosis, and monitoring of skin disease in vivo. Fluorescent confocal microscopy is an innovative optical...
Article
In this 2-part article, the authors review the primary cicatricial alopecias. Primary cicatricial alopecia can be defined as predominantly lymphocytic, neutrophilic, or mixed based on the nature of the follicular infiltrate that is present around affected hair follicles. Lymphocytic primary cicatricial alopecias include chronic cutaneous lupus eryt...
Article
Androgenetic alopecia (AGA), or male pattern hair loss, affects approximately 50% of the male population. AGA is an androgen-related condition in genetically predisposed individuals. There is no treatment to completely reverse AGA in advanced stages, but with medical treatment (eg, finasteride, minoxidil, or a combination of both), the progression...
Article
Optical, non-invasive methods, such as fluorescence laser scanning microscopy (LSM) and optical coherent tomography (OCT), have become efficient tools for the characterization of the skin structure in vivo, as well as real-time investigation of distribution and penetration of topically applied substances. In the present paper, the results obtained...
Article
Follicular drug delivery is the prerequisite for an effective treatment of androgenetic alopecia or other reasons of premature hair loss. The follicular penetration of caffeine, applied topically in a shampoo formulation for 2 min, was measured with highly sensitive surface ionization in combination with mass spectroscopy, a selective method for th...
Article
Full-text available
The penetration and storage behavior of dye-containing nanoparticles (diameter 320 nm) into the hair follicles was investigated. The results were compared to the findings obtained with the same amount of dye in the non-particle form. In the first part of the experiments, the penetration of the dye into the hair follicles was investigated in vitro o...
Article
Porcine ear skin is used in studies of percutaneous penetration as a substitute for human skin. The structure of this tissue, including hair follicles, was studied qualitatively and quantitatively in comparison with human skin. Sections of shock-frozen biopsies, biopsies embedded in paraffin and cyanoacrylate skin surface biopsies were investigated...
Article
Full-text available
Liposomes had been widely used for drug delivery in the past. In this study, five different liposomes were used as a follicular delivery system in pig ear skin. The liposomes mainly differed in their sphere diameter, lipid composition, and surface charge. A novel class of liposomes being amphoteric in their charge behavior are compared to establish...
Article
Optical, noninvasive methods have become efficient in vivo tools in dermatological diagnosis and research. From these promising imaging techniques, only the confocal scanning laser microscopy (CSLM) provides visualization of subsurface skin structures with resolutions similar to those of light microscopy. Skin annexes, as well as cutaneous cells fr...
Article
Confocal scanning laser microscopy (CSLM) constitutes an optical, noninvasive method providing visualization of tissue architecture with resolution similar to that of light microscopy. In dermatology, confocal imaging enables in vivo measurements of surface and subsurface skin microstructures. Skin annexes, as well as cutaneous cells from different...
Article
Full-text available
Nanoparticles represent an important drug carrier system. Recently, we have reported on the penetration and storage behavior of particular and non-particular substances revealing the superiority of particular substances in the range of 300-400 nm. In this regard, it was assumed that the rigid hair shaft acts as a geared pump, moving the particles d...
Article
It is a given fact that hair follicles play an important role in the penetration process of topically applied drugs and cosmetics. Since exact knowledge of the potential follicular reservoir is essential for understanding and calculating the penetration process, this knowledge may indeed help to optimize the development of topically applied drugs a...
Article
Investigations into the penetration pathways of topically applied substances through the skin play an important role in dermatological science. Recently, the hair follicles have increasingly been recognized as an important pathway for percutaneous penetration, although the role of the follicles has still not been clarified in detail. Therefore, the...
Article
In the past, intercellular penetration was assumed to be the most important penetration pathway of topically applied substances. First hints that follicular penetration needs to be taken into consideration were confirmed by recent investigations, presented during the workshop "Follicular Penetration and Targeting" at the 4th Intercontinental Meetin...
Article
Fiber-based confocal laser scanning microscopy affords a vast field of application in medical research and clinical practice. The application of fluorescent dye allows real-time imaging of yeasts of the genus Malassezia on human skin in vivo. An Ar⁺-laser is used to excite the fluorescent food dye sodium fluorescein at 488 nm. Its emission is simul...
Article
Optical coherence tomography (OCT) was used to research skin properties after 5 different pretreatments. Untreated and artificially dried skin was investigated in vivo by OCT. The effects and distribution of moisturizing cream and sunscreen were observed Additionally, it was possible to visualize the effects of topically applied clobetasol and of U...
Article
Eine wesentliche Voraussetzung für die qualitative und quantitative Beurteilung von Penetrationsprozessen in und durch die Haarfollikel ist die Kenntnis von Morphologie und Verteilung von Haarfollikeln am menschlichen Körper. In der Literatur finden sich nur wenig Untersuchungen zur Verteilung und Morphologie von Haarfollikeln. Anhand von Cyanacryl...
Article
The efficacy of the penetration of microparticles into the human skin depends on the size and the type of the formulation with which they are topically applied. Microparticles with a diameter of >1 microm barely penetrate into the human skin. They are located on the skin surface and form a film which, for instance, can be used for camouflage or pro...
Article
Die Effektivität der Penetration von Mikropartikeln in die menschliche Haut hängt in entscheidendem Maße von ihrer Größe und der Art der Formulierung ab, in der sie appliziert werden. Mikropartikel von >1 µm dringen kaum in die Haut ein und sind an der Oberfläche lokalisiert. Sie eignen sich daher sehr gut zum Abdecken von Hautoberflächen, wie z. B...
Article
For the evaluation and quantification of follicular penetration processes, the knowledge of variations of hair follicle parameters in different body sites is basic. Characteristics of follicle sizes and potential follicular reservoir were determined in cyanoacrylate skin surface biopsies, taken from seven different skin areas (lateral forehead, bac...
Article
The erythema protection efficacy of sunscreens is characterized by the sun protection factor (SPF), which is related to the UVB part of the solar spectrum. UVA protection, which is an important factor in immune suppression, is not taken into account by the SPF. The combination of the suction blister technique and UV spectroscopy allows the determin...
Article
Hair follicles have been assigned an increasingly significant role in dermatopharmacology. Previous studies have shown that the penetration of substances into the follicle depends on its activity. Telogen follicles that were not producing sebum were closed for follicular penetration. The present study uses optical coherence tomography (OCT) to show...
Article
The state of the art of in-vivo and in-vitro penetration measurements of topically applied substances is described. Only optical techniques represent online measuring methods based on the absorption or scattering properties of the topically applied substances. Laser scanning microscopy (LSM) has become a promising method for investigations in derma...
Article
The state of the art of in-vivo and in-vitro penetration measurements of topically applied substances is described. Only optical techniques represent online measuring methods based on the absorption or scattering properties of the topically applied substances. Laser scanning microscopy (LSM) has become a promising method for investigations in derma...
Article
Full-text available
The knowledge of penetration pathways in and through the skin is a prerequisite for the development and optimization of topically applied drugs and cosmetics. Skin penetration has been assumed to occur via diffusion though the lipid domains of the stratum corneum. Recent studies show that the skin appendages, especially the hair follicles, play an...
Article
The dermatological laser scanning confocal microscope, Optiscan Stratum, was applied to in vivo investigations in skin physiology. The radiation of an Ar+ laser at 488 nm was used to excite the topically applied food dyes curcumin and sodium fluorescine. The radiation was transferred by an optical fiber to a hand piece set directly onto the skin su...
Article
Laser scanning microscopy in combination with cyanoacrylate skin surface biopsy was used to observe follicular penetration of topically applied curcumin emulsion into the hair follicle. The dye was found in most of the hair follicles, although some follicles were obviously closed to penetration. Reasons for these different penetration characteristi...
Article
The influence of specific follicle properties, sebum production and hair growth on the follicular penetration of topically applied substances was investigated. The behavior of follicles identified in selected skin areas of volunteers was analyzed by various tape stripping and staining methods in combination with laser scanning microscopy. Furthermo...

Citations

... The link between androgens and hair loss is known and recently has come into more focus in the era of COVID-19 and hair loss due to the fact that Coronavirus appears to elicit androgenic responses [4,5]. Hair loss can be triggered by drugs and radiation as well as hormonal imbalance [7,8]. ...
... e maintenance dose varies between individuals and usually ranges from biweekly to bimonthly treatments. [13] is maintenance has to be continued as long as patient requires it to remain in remission. ...
... 5,12,16 Similar to other chemotherapeutic agents, it is thought to cause alopecia through anagen effluvium because of its cytostatic effects. 19 It Figure 2 The occurrence of skin hyperpigmentation 1 month after initiation of the hydroxyurea generally presents on the scalp and may be partial or diffuse. 5,12 Although one study reported an average latency period of 8 weeks, it has also occurred several years after HU therapy. ...
... The basis for the diagnosis of alopecia, including non-scarring alopecia, are clinical presentation and trichoscopy, while in doubtful situations a biopsy should be taken for histological examination [6,7]. Trichoscopy, or dermoscopy of hair and scalp, is a non-invasive diagnostic method in which a hand-held dermoscope or videodermoscope is used to assess various hair disorders [8]. High-frequency ultrasonography (HF-USG) is another non-invasive diagnostic method that has been applied in dermatology for many years [9]; however, its usefulness for the assessment of different forms of alopecia has not been widely investigated; there are only single reports referring to the subject [10][11][12]. ...
... The dimensions and physiology of individual scalp hair is well known [19] but thickness of compressed hair layer will be highly related to physical characteristics. Thereby, sample size for systematic studies will be greatly affected by hair type due to worldwide variation and classification of human hair [20,21]. In such program, a map should be constructed between hair layer thickness and hair type to be assessed by objective and simple measurements to know how to design close fitting head mounted products for multiple groups with design guidelines in function of hair type rather than ethnicity [22]. ...
Citing conference paper
... Some experiments were performed recently in vivo to reveal the protection effect of administered sunscreens, but disturbing living tissue with detectors situated between epidermis and dermis. 26 The detectors collect photons coming from the horny layer into the air or epidermis. In this paper, we consider only "unshaded" effect of the horny layer with TiO 2 nanoparticles added, not taking into account deeper localized skin layers as scattering and absorbing media. ...
... It is a potent and highly sensitive selective 5α-reductase type-2 inhibitor (8) . The side effects include decreased libido in 1.8% of the recipients vs. 1.3% in the placebo group, erectile dysfunction in 1.3% of the recipients vs. 0.7% in the placebo group and decreased ejaculation volume in 0.8% of the recipients vs. 0.4% in the placebo group (8,9) . The sexual side effects are usually reversible. ...
... Therefore, samples can also be analyzed within the z-plain more conveniently and various deep sections may be obtained [174]. Consequently, the experimental setup works for in vivo determinations; for instance, Lademann et al. retraced the FP of curcumin and sodium fluorescein in human volunteers [175]. In vitro application on porcine ear skin is also applicable; for instance, Subongkot et al. determined the transfollicular penetration of sodium fluoresceinloaded ultradeformable liposomes via CLSM [176]. ...
... This led to the conclusion that the hair follicles must have been blocked by a plug that must be removed before the externally applied substance could penetrate. 11 Such a plug was successfully detected using optical coherence tomography, and it was shown that it consisted of desquamated corneocytes and dried sebum. 12 These can be easily removed by washing or mild peeling, so as to render the hair follicles receptive for penetration in the respective skin areas. ...
... Recently, cross-polarized UV reflectance imaging was reported as a further option for qualitative investigation [18,19]. For titanium dioxide containing sunscreens, light microscopy on cross sections of skin biopsies [20] or in vivo optical coherent tomography [21] allowed a rough estimation of the thickness of the layer based on the visualization of titanium dioxide particles. Most of the above-mentioned assays were done in vivo. ...