Nehad’s scientific contributions

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Publications (3)


Molecular cloning and sequence analysis of vira-like chitinase gene from the cotton leaf worm, Spodoptera littoralis Bosid (Lepidoptera: Noctuidae), treated with two insect growth regulators
  • Article
  • Full-text available

July 2015

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151 Reads

International Journal of Pharmacy and Biological Sciences

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Mona F Abd Elaziz

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Nehad

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[...]

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The vira-like chitinase gene, one of the genes involved in the formation of chitin and concerned with development of insect. PCR-RFLP technique was used in the present study to compare the effect of sub-lethal doses of two IGRs, flufenoxuron (cascade) as a chitin synthesis inhibitor and tebufenozide (mimic) as a moulting hormone agonist, on this gen in Spodoptera littoralis pest. As the 2 nd instar larvae were treated with sublethal doses LC 25 , LC 50 and LC 90 of the two tested IGRs. The endonucleases Spel, EcoRI and Bbsl were used to detect the molecular changes in the gene at different time intervals 6, 12, 24 and 48 hours post treatment. Bioinformatical obtained results showed significant changes in the gene sequences (mutations) of the treated groups which were dose and time dependants. For each studied group, the RNA secondary structure stems nucleotide sequences; positions and free energy were recorded.

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Fig. 1: Shows the structure of the predicted RNA structure of the normal and 1 st group.  
Fig. 2: Shows the structure of the predicted RNA structure of 2 nd group.  
Fig. 3: Shows the structure of the predicted RNA structure of 3 rd group.  
Fig. 4: Shows the structure of the predicted RNA structure of 4 th group.  
Table 4 : Groups of mutated vira-like chitinase gene resulted from the treatments of the 2 nd larval instar of S. littoralis with the different sub lethal doses of match at the different times intervals post treatments..
Biological and molecular aspects of the insect growth regulator lufenuron on Spodoptera littoralis (Bosid) (Lepidoptera:Noctuidae)

December 2014

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132 Reads

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1 Citation

Egyptian Academic Journal of Biological Sciences C Physiology and Molecular Biology

The cotton leafworm, S. littoralis, is a major pest in Egypt causes severe quantitative and qualitative losses of cotton and other economic crops. This study examined the effect of chitin synthesis inhibitors, lufenuron (match 5%) on the 2nd instar larvae. The results showed that lufenuron caused not only mortality in larval stages, but also caused effect on pupal and adult duration, adult fecundity and egg fertility. Also lufenuron treatments have resulted in emergence of deformed pupae and adults. PCR-RFLP technique was used in this study to investigate the effect of lufenuron on the vira-like chitinase gene, one of the genes involved in the formation of chitin and concerned with development of insect. The endonucleases Spel, EcoRI and Bbsl were used to detect the molecular changes at different time intervals 6, 12, 24 and 48hrs post treatment with sublethal doses LC25, LC50 and LC90.The results showed significant changes in the sequences of the treated groups which were dose and time dependants. Moreover the number of RNA secondary structure stems was varied in the different studied groups and most of them differed in their nucleotide sequences, positions and free energy.


Citations (1)


... The available literature contains many reported results of toxic effects of several IGRs (Juvenoids, ecdysteroids and chitin synthesis inhibitors, CSIs) on various insect species, such as Spodoptera littoralis by Diflubenzuron (Aref et al., 2010)[36], Triflumuron (Bakr et al., 2010; El-Sheikh and Ashour, 2011)[37,38], Chlorfluazuron (Bayoumi et al., 1998)[39], Flufenoxuron (El-Naggar, 2013)[40], Lufenuron (AbdelRahman et al., 2007;Adel, 2012;Gaaboub et al., 2012;Bakr et al., 2013)[41][42][43][44], Buprofezin (Nasr et al., 2010; Ragaei and Sabry, 2011)[45,46], Tebufenozide and Methoxyfenozide (Pineda et al., 2004)[47], Cyromazine (Tanani et al., 2015)[48], etc. Also, various IGRs exhibited toxic effects against other insects, such as Tebufenozide and Methoxyfenozide against Choristoneura fumiferana (Sundaram et al., 2002)[49]; Diofenolan against Musca domestica (Hamadah, 2003)[50]and Papilio demoleus (Singh and Kumar, 2011)[51]; Pyriproxyfen against Eurygaster integriceps (Mojaver andBandani, 2010)[52]; Flufenoxuron against Dysdercus koenigii (Khan and Qamar, 2011)[53]; Diflubenzuron against Halyomorpha halys (Kamminga et al., 2012)[54]; Chlorfluazuron against Spodoptera litura (Perveen, 2012)[55]; Flufenoxuron, RH-5849 and Pyriproxyfen against Locusta migratoria var. ...

Reference:

Toxic and disruptive effects of Novaluron, a chitin synthesis inhibitor, on development of the pink bollworm Pectinophora gossypiella (Saunders)(Lepidoptera: Gelechiidae).
The Activity of Some Detoxification Enzymes in Spodoptera Littoralis (Boisd.) Larvae (Lepidoptera – Noctuidae) Treated With Two Different Insect Growth Regulators
  • Citing Article
  • January 2013