Nan Zhang's research while affiliated with Yangzhou University and other places

Publications (26)

Article
As the downstream effector of the target of rapamycin complex 1 (TORC1) signaling pathway, the ribosomal protein S6 kinase (S6K) is an important regulator of insect reproduction, however, the underlying mechanism remains obscure. In this study, a S6K gene, named TcS6K1, was isolated from the red flour beetle, Tribolium castaneum. Analysis of tempor...
Article
Intermittent food scarcity is commonly encountered in all organisms including insects. Although the forkhead box O (FOXO) transcription factor is a well‐known component of signalling cascades related to starvation stress, the detailed regulatory mechanisms during long‐term starvation remain poorly understood in insects. In the present study, using...
Article
Metabolism of insecticides is an energy-consuming process. As an important component of the intracellular energy buffering system, arginine kinase (AK) plays an important role in insect cellular energy homeostasis and environmental stress response, but the involvement of AKs in the response to chemical stressors (insecticides) remains largely unkno...
Article
The glutathione S-transferases (GSTs) are a kind of metabolic enzymes and participate in the detoxification metabolism of xenobiotics in various organisms. In insects, GSTs play important roles in the development of insecticide resistance and antioxidant protection. The rice stem borer Chilo suppressalis is one of the most damaging pests in rice an...
Article
Background: Both forkhead box O (FOXO) and nuclear factor erythroid-derived 2-like-2 (Nrf2) are key transcription factors related to stress response. While limited studies in mammals and Caenorhabditis elegans revealed interaction between FoxO/DAF-16 and Nrf2/SKN-1, the role of FOXO in metabolic detoxification and regulation of Nrf2-Keap1 signalin...
Article
The sulfuryl transfer reaction catalyzed by cytosolic sulfotransferase (SULT) is one of the major conjugating pathways responsible for the detoxification and subsequent elimination of xenobiotics, however, functional characterization of insect SULTs is still limited. In this study, cDNA encoding a cytosolic sulfotransferase, named TcSULT1, was clon...
Article
The insect voltage-gated sodium channel is the primary target of pyrethroids and novel efficient insecticides such as indoxacarb and metaflumizone. In this study, we cloned and characterized two putative sodium channel genes, TcNav1 and TcNav2, in Tribolium castaneum. The composite TcNav1 and TcNav2 encode a protein of 2045 and 2037 amino acid resi...
Article
Full-text available
Background Short interspersed nuclear elements (SINEs) belong to non-long terminal repeat (non-LTR) retrotransposons, which can mobilize dependent on the help of counterpart long interspersed nuclear elements (LINEs). Although 234 SINEs have been identified so far, only 23 are from insect species (SINEbase: http://sines.eimb.ru/ ). Results Here, f...
Article
The oxidation of methionine (Met) by reactive oxygen species (ROS) causes detrimental effects on the protein functions. Methionine sulfoxide reductase (Msr) is the secondary antioxidant enzyme involved in protein repair, and is divided into two distinct classes, MsrA and MsrB, although the mechanisms underlying the transcriptional regulation of Msr...
Article
BACKGROUND The selective insecticide flonicamid shows highly insecticidal activities against piercing‐sucking insects and has been widely used for the control of Hemipteran insect pests, whereas its effects on Lepidopteran insect pests remain largely unknown. Recently, inward rectifier potassium (Kir) channel has been verified to be a target of flo...
Article
Full-text available
The phosphoarginine-arginine kinase shuttle system plays a critical role in maintaining insect cellular energy homeostasis. Insect molting and metamorphosis are coordinated by fluctuations of the ecdysteroid and juvenile hormone. However, the hormonal regulation of insect arginine kinases remain largely elusive. In this report, we comparatively cha...
Article
Full-text available
Background: The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In t...
Article
Full-text available
Besides the function of preventing metamorphosis in insects, the juvenile hormone (JH) plays a role in female reproduction; however, the underlying mechanism is largely unknown. The methoprene-tolerant (Met) protein belongs to a family of basic helix-loop-helix–Per-Arnt-Sim (bHLH-PAS) transcription factors and functions as the JH intracellular rece...
Preprint
Full-text available
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In thi...
Preprint
Full-text available
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In thi...
Preprint
Full-text available
Background The AMP-activated protein kinase (AMPK) is an intracellular fuel sensor for lipid and glucose metabolism. In addition to the short-term regulation of metabolic enzymes by phosphorylation, AMPK may also exert long-term effects on the transcription of downstream genes through the regulation of transcription factors and coactivators. In thi...
Article
As a member of the low‐density lipoprotein receptor (LDLR) superfamily, vitellogenin (Vg) receptor (VgR) is responsible for the uptake of Vg into developing oocytes and is a potential target for pest control. Here, a full‐length VgR complementary DNA (named as CsVgR) was isolated and characterized in the rice stem borer, Chilo suppressalis. The com...
Article
The AMP-activated protein kinase (AMPK) has important roles in the regulation of energy metabolism, and AMPK activity and its regulation have been the focus of relevant investigations. However, functional characterization of AMPK is still limited in insects. In this study, the full-length cDNA coding AMPKα (TcAMPKα) was isolated from the red flour...
Article
Full-text available
Reversible acetylation of core histones plays an important role in the epigenetic regulation of gene transcription, and is controlled by the action of histone acetyltransferases (HATs) and histone deacetylases (HDACs). While HDACs have been well studied in Drosophila melanogaster, information from insect pests is still limited. In the current study...
Article
While sublethal effects of insecticide on insect development have been widely studied, the underlying mechanisms remain elusive. Our previous studies revealed that sublethal concentrations of chlorantraniliprole significantly increased the juvenile hormone levels and resulted in both prolonged developmental time and reduced fecundity in Chilo suppr...
Article
Full-text available
Background: Helitrons play an important role in shaping eukaryotic genomes due to their ability to transfer horizontally between distantly related species and capture gene fragments during the transposition. However, the mechanisms of horizontal transfer (HT) and the process of gene fragment capturing of Helitrons still remain to be further clarif...
Article
Arginine kinase (AK) plays a critical role in insect energy metabolism and has been proposed to be a potential insecticide target for commercial exploitation. In this study, the full length cDNA encoding a typical group 1 insect AK (FoAK) was isolated from the western flower thrips (WFT), Frankliniella occidentalis (Pergande). Sequence analysis sho...
Article
The γ-aminobutyric acid (GABA) receptor is a primary neurotransmitter receptor in both vertebrate and invertebrate nervous systems. Multiple RDL subunits have been found in insects including the rice stem borer, Chilo suppressalis, however, comparative characterization of duplicated RDL genes in insects is still limited. In this study, comparison o...
Article
To understand the role of target site insensitivity in abamectin resistance in the western flower thrips (WFT), Frankliniella occidentalis (Pergande), cDNAs encoding gamma-aminobutyric acid receptor subunit (FoRdl) and glutamate-gated chloride channel (FoGluCl) were cloned from WFT, and both single nucleotide polymorphisms (SNPs) and mRNA expressio...
Article
Background: Juvenile hormone (JH) regulates development and reproduction of insects. The sublethal effects of chlorantraniliprole on JH levels and mRNA expression of JH acid methyltransferase gene (CsJHAMT) and farnesyl diphosphate synthase genes (CsFPPS1 and CsFPPS2 ) in Chilo suppressalis (Walker) were investigated. Results: Exposure of sublet...

Citations

... It is suggested that four genes may be involved in the detoxification process of E. oblique. It has been reported that arginine kinase TcAK1 in Tribolium castaneum significantly increased its mRNA level at 2 h and 4 h after deltamethrin stimulation, but recovered to the control level at 12 h, which indicated that different enzyme genes had different response mechanisms to deltamethrin stimulation [31]. ...
... Detoxification of specific molecules is one of the mechanisms to adapt to resistance. It involves reduction of substrate toxicity by cytochrome P450 monooxygenases (Scott and Wen 2001;Wu et al. 2021), conversion of hydrophobic toxic compounds to hydrophilic products by glutathione-S-transferases, uridine diphosphate glucuronosyl transferases and carboxyl esterases (Pan et al. 2019;Hilliou et al. 2021;Meng et al. 2022), and efflux of conjugated xenobiotics from the cell mainly by the ATP-binding cassette (ABC) transporters (Dawkar et al. 2013;Hilliou et al. 2021). ...
... The dsC/EBPα gene was injected into one-dayold P. xylostella fourth-instar larvae using microinjection tools (WPI, Sarasota, FL, USA). dsEGFP was used as the negative control [35]. Each larva was injected with 150 ng dsRNA, and a sample of three injected larvae was collected after 24 h for quantitative real-time PCR analysis. ...
... While SINEs depend on other TEs for their retrotransposition, evidence of SINE HT is present in the literature. The relatively young 5S RNA-derived SINE PxSE4 shows evidence of HT in lepidopteran insects [51], and novel HaSE SINEs show signatures of HT in the cotton bollworm, Helicoverpa armigera [52]. Indicators of HT have also been found for a SINE shared by reptiles and mammals and the SmaI SINE family in salmonid fishes [53,54]. ...
... We thus treated B. straminea with 10 -6 M ecdysteroid 20hydroxyecdysone for 24 hours but did not observe any significant expression changes in the downstream genes E74, FOXO, and Nvd. Similar hormone treatments have been shown to elicit the downstream genes in insects in previous studies [37][38][39]. It is unclear whether only certain forms of ecdysteroids may induce endogenous ecdysteroid pathway genes under particular conditions, and this warrants further investigation. ...
... The genes were initially denominated Drosophila inward rectifier (Dir or ir), inward rectifier K + 2 (irk2), and irk3 (19, 44). Subsequently, orthologs of Dir, irk2, and irk3 were identified in other Diptera (mosquitoes), Hemiptera, and Lepidoptera, where distinct subtypes and/or splice variants of irk2 and irk3 occur in some species (see Section 2.2) (15, 36,39,45,47,48,60,61,63,66). To avoid confusion with ionotropic receptors (Ir) and more closely align with naming conventions of vertebrate Kir genes, we revised the nomenclature of insect Kir genes as follows: Dir/ir to Kir1, irk2 to Kir2, and irk3 to Kir3 (61). ...
... AK is commonly known as an energy metabolizer for muscle contraction [20]. AK is also involved in ecdysone regulation in Drosophila [27] and adult fertility and stress response in Tribolium castaneum Herbst (Coleoptera: Tenebrionidae) [28]. We observed that the thorax and abdominal regions of P. xylostella larvae appeared to exhibit typical shrinkage, some larvae failed to exuviate and some pupae could not break out after 8010AKi or BMB171AKi treatment, which was probably caused by the disorder of energy metabolism for muscle development after PxAK was inhibited. ...
... Conversely, the regulation mechanism of AMPK in trehalose metabolism and chitin synthesis remains poorly understood in insects. A recent study indicated that both RNAi-mediated AMPKα silencing and AMPK activator, 5-Aminoimidazole-4-carbox-amide1-β-D-ribofuranoside (AICAR) significantly influenced glucose and trehalose levels in Tribolium castaneum, as well as the transcriptional levels of carbohydrate metabolism-related genes (Jiang et al., 2020). Similarly, we observed another AMPK activator metformin significantly disturbed carbohydrate metabolism in H. cunea larvae (Fig. 9), suggested AMPK plays a critical role in regulating carbohydrate metabolism. ...
... Vg is primarily synthesized in insect body fat, then secreted into the haemolymph and utilized by oocytes through a complicated physiological process [3]. The reproductive success of insects depends on Vg biosynthesis and uptake by developing oocytes [4]. Therefore, Vg can be used as a molecular marker of fecundity [5]. ...
... These observations suggested that HmVgR gene was highly expressed long before vitellogenesis started. However, this expression pattern was different from hemimetabolous and some holometabolous insects (e.g., Lepidoptera), whose VgRs mRNA were highly detected in nymph and pupa stages, respectively (Shu et al. 2011, Miao et al. 2020. The initiation of VgR transcription level varies among insect species, but they were related to the formation of ovary and initiation of vitellogenesis. ...