Najah Salman Abd’s scientific contributions

Several studies have shown that the presence of Helicobacter pylori (H. pylori) may be a risk factor for Ischemic Heart Diseases (IHD). Therefore, it is needed to look into the relation between IHD and H. pylori infection. One hundred Iraqi patients with IHD and 60 healthy Iraqis as a control group participated in the current study. The ELISA assay was used for the measurement of the sera levels for anti-H. pylori IgG, IgM, and Interleukin-6 (IL-6). Also, H. pylori DNA in the human venous blood was investigated by the polymerase chain reaction (PCR) using 16S rDNA primers. Between IHD patients and the control group, H. pylori-IgG and IL-6 mean ± SD were (Patient =1.814±0.08, Control = 0.823±0.09, Patient = 49.73±6.60, Control = 0.21±0.02, respectively), and this results showed a highly significant variation (p < 0.05) in anti-H. pylori IgG and IL-6, while no significant variations (p > 0.05) were found in anti-H. pylori IgM. It was also found that PCR amplification of Helicobacter pylori DNA using 16S rDNA primers was positive in the venous blood of 81 (81%) IHD patients. The current study results proposed that there was a relation between H. pylori and Ischemic Heart Disease. ‫من‬ ‫لكل‬ ‫المتوسط‬ ‫ان‬ ‫النتائج‬ ‫أظهرت‬ ‫المتسلسل.‬ ‫البلمرة‬ ‫تفاعل‬ ‫اسطة‬ ‫بو‬ IgG ‫و‬ IL-6 ‫كانت‬ (P=1.814±0.08 , C=0.823±0.09 , P=49.73±6.60 , C=0.21±0.02 ‫الي‬ ‫التو‬ ‫على‬ , ‫داللة‬ ‫ذات‬ ‫فروق‬ ‫وجود‬ ‫على‬ ‫تدل‬ ‫النتائج‬ ‫وهذه‬ ,) ‫إحصائية‬ (p <0.05) ‫ابية‬ ‫البو‬ ‫الملوية‬ ‫جرثومة‬ ‫مضاد‬ ‫في‬ (IgG) ‫في‬ ‫وكذلك‬ (IL-6) ‫االقفاري‬ ‫القلب‬ ‫بمرض‬ ‫المصابين‬ ‫المرضى‬ ‫بين‬ ‫فروق‬ ‫هناك‬ ‫تكن‬ ‫لم‬ ‫بينما‬ ‫الضابطة‬ ‫المجموعة‬ ‫و‬ ‫إحصائية‬ ‫داللة‬ ‫ذات‬ (p > 0.05) ‫ابية‬ ‫البو‬ ‫الملوية‬ ‫جرثومة‬ ‫مضاد‬ ‫في‬ (IgM) ‫المرضى‬ ‫بين‬ ‫المتسلسل‬ ‫البلمرة‬ ‫تفاعل‬ ‫اسطة‬ ‫بو‬ ‫المتضخم‬ ‫ابية‬ ‫البو‬ ‫للملوية‬ ‫النووي‬ ‫الحمض‬ ‫أن‬ ‫ا‬ ً ‫أيض‬ ‫وجد‬ ‫الضابطة.‬ ‫المجموعة‬ ‫و‬ ‫االقفاري‬ ‫بالقلب‬ ‫المصابين‬ ‫بادئات‬ ‫باستخدام‬ (16S rDNA) ‫ال‬ ‫الدم‬ ‫في‬ ‫ا‬ ً ‫إيجابي‬ ‫كان‬ ‫ل‬ ‫محيطي‬ 81 (1 8 ‫نتائج‬ ‫اقترحت‬ ‫اإلقفارية.‬ ‫القلب‬ ‫اض‬ ‫بأمر‬ ‫ا‬ ً ‫مريض‬)% ‫اإلقفارية.‬ ‫القلب‬ ‫اض‬ ‫أمر‬ ‫و‬ ‫ابية‬ ‫البو‬ ‫الملوية‬ ‫بجرثومة‬ ‫اإلصابة‬ ‫بين‬ ‫عالقة‬ ‫هناك‬ ‫أن‬ ‫الحالية‬ ‫اسة‬ ‫الدر‬ ‫المفتاحية:‬ ‫الكلمات‬ , ‫االقفاري‬ ‫القلب‬ ‫مرض‬ ‫ليوكين‬ ‫انتر‬-6 ‫ال‬ ‫النووي‬ ‫الحمض‬ ‫ا,‬ ‫االليز‬ ‫المتسلسل,‬ ‫البلمرة‬ ‫,تفاعل‬ ‫ر‬ ‫ا‬ ‫ال‬ ‫منقوص‬ ‫يبوزي‬ ‫و‬ ‫كسجين‬ .

Sixty five Ischemic heart disease (IHD) patients and 25 healthy control were included in this work. 90.7% hypertensive patients and 66% of patients were with diabetes. Serum samples were tested for interleukin-17A (IL-17A), interleukin-17A Receptor (IL-17AR) and interleukin-12/23p40 (IL-12/23p40) using Sandwich ELISA technique, Mean serum levels as compared to healthy of 17A, IL-17AR and IL-12/23p40 were 57.5±2.6 pg/ml, 37.3±5.1 pg/ml and 301±43.3 pg/ml compared to healthy control groups 11.7±1.7pg/ml, 7.3±1.0pg/ml and 10.4±3.6pg/ml respectively. It could be concluded that the prevalence of IHD in a diabetic patients is higher than control, IL-17A, IL-17AR and IL-12/23p40 have a role in diagnosis, and IL-12 and IL-23 play a crucial role in pathogenesis of IHD.