Na Zhu's research while affiliated with Chinese Center For Disease Control And Prevention and other places

Publications (17)

Article
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There is an urgent need for animal models to study SARS-CoV-2 pathogenicity. Here, we generate and characterize a novel mouse-adapted SARS-CoV-2 strain, MASCp36, that causes severe respiratory symptoms, and mortality. Our model exhibits age- and gender-related mortality akin to severe COVID-19. Deep sequencing identified three amino acid substituti...
Article
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SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood. Here, we characterize the replication dynamics, cell tropism and morphogenesis of SARS-CoV-2 in organotypic human airway epithelial (HAE) cultures. SARS-CoV-2 replicate...
Article
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Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) caused the corona virus disease 2019 (COVID-19) cases in China and has become a public health emergency of international concern1. Because angiotensin-converting enzyme 2 (ACE2) is the cell entry receptor of SARS-CoV5, we used transgenic mice bearing human ACE2 and infected with SARS-CoV-2 to stu...
Preprint
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2019-nCoV caused pneumonia cases in China has become a public health emergency of international concern (PHEIC). The first priority for prevention and treatment of the disease is to find the pathogenicity of 2019-nCoV in vivo. Weight loss and virus replication were detected in infected-hACE2 mice. The typical histopathology was interstitial pneumon...
Article
Background: In late December, 2019, patients presenting with viral pneumonia due to an unidentified microbial agent were reported in Wuhan, China. A novel coronavirus was subsequently identified as the causative pathogen, provisionally named 2019 novel coronavirus (2019-nCoV). As of Jan 26, 2020, more than 2000 cases of 2019-nCoV infection have be...
Article
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In December 2019, a cluster of patients with pneumonia of unknown cause was linked to a seafood wholesale market in Wuhan, China. A previously unknown betacoronavirus was discovered through the use of unbiased sequencing in samples from patients with pneumonia. Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV,...
Article
Currently, there is no approved therapy to treat coronavirus infection; therefore, broad-spectrum inhibitors of emerging and endemic CoVs are needed. Based on our high-throughput screening assay using a compound library, we identified seven compounds with broad-spectrum efficacy against the replication of four CoVs in vitro . Additionally, one comp...
Article
[Figure not available: see fulltext.] © 2016 Wuhan Institute of Virology, CAS and Springer Science+Business Media Singapore
Article
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Severe acute respiratory infection (SARI) in children is thought to be mainly caused by infection with various viruses, some of which have been well characterised; however, analyses of respiratory tract viromes among children with versus without SARI are limited. In this study, nasopharyngeal swabs from children with and without SARI (135 vs. 15) w...
Article
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Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel and highly pathogenic human coronavirus and has quickly spread to other countries in the Middle East, Europe, North Africa and Asia since 2012. Previous studies have shown that MERS-CoV ORF4b antagonizes the early antiviral alpha/beta interferon (IFN-α/β) response, which may signifi...
Article
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No comprehensive analysis is available on the viral etiology and clinical characterization among children with severe acute respiratory infection (SARI) in China during 2009 H1N1 pandemic and post-pandemic period. Cohort of 370 hospitalized children (1 to 72 months) with SARI from May 2008 to March 2010 was enrolled in this study. Nasopharyngeal as...
Article
Full-text available
Introduction Integration-deficient lentiviral vectors (IDLVs) are a promising platform for immunisation to elicit both humoral immunity and cellular mediated immunity (CMI). Here, we compared the specific immunity in mice immunised via different regimens (homologous and cocktail) with IDLV-based HCV pseudoparticles (HCVpps) carrying pseudotyped gly...
Article
Full-text available
Acute respiratory tract infections (ARTIs) represent a serious global health burden. To date, few reports have addressed the prevalence of respiratory viruses (RVs) in adults with ARTIs attending an emergency department (ED). Therefore, the potential impact of respiratory virus infections on such patients remains unknown. To determine the epidemiol...

Citations

... Notably, the S protein of a lethal mouse-adapted SARS-CoV-2 strain, MASCp36, also carried a Q493H substitution, which emerged after N501Y during in vivo passage in mice, increasing the binding affinity to mACE2 by 27.8fold (K D of 353.4 μM vs 12.67 μM) 33 . A structural analysis of MASCp36 H493 revealed an H-bond with N31 and a salt bridge with E35 of mACE2 (Fig. 4c). ...
... A number of studies on SARS-CoV-2-induced apoptosis in respiratory epithelial cells and ECs align with the above findings. [49][50][51][52] A large number of the renal tubular epithelial cells also occur apoptosis, leading to AKI in the mouse model with SARS-CoV-2 infection. 53 In addition, SARS-CoV-2 induced β cell apoptosis via the JNK-MAPK apoptotic pathway, resulting in attenuated insulin production and secretion and exacerbation of diabetes. ...
... The Ad5-hACE2-transduced mice did not cause severe disease and extrapulmonary manifestations of disease [34]. Typical histopathology of lung, weight loss, virus replication and upregulated innate immune response have been observed in human ACE2transgenic mouse models [35,36]. However, only K18-hACE2 mice could develop severe disease and cause high lethality [36,37]. ...
... Several NNAs have been studied in targeting viral polymerase against hepatitis C virus (HCV), ZIKA (43), and human immunodeficiency virus (HIV) infections. In recent studies, an NNA lycorine was reported to inhibit diverse coronavirus infections such as SARS-CoV, MERS-CoV, HCoV-NL63, HCoV-OC43 and SARS-CoV-2 both in vitro and in vivo (44)(45)(46). Therefore, it might be more promising and pressing to develop novel non-nucleoside analog drugs that bind to the SARS-CoV-2 RdRp complex and cause an allosteric inhibition of virus replication and transcription. ...
... Phylogenetically, the 2019 novel CoV originated from the genus β-CoV, which includes coronaviruses (SARS/SARS-like -CoV and others) identified in bats, humans, and few wild animals [71]. The spike (S) proteins, crucial for host tropism and transmission capacity, mediates receptor binding through its domain S1 and plasma membrane fusion through S2. S protein of SARS-CoV-2 shared high sequence similarity (88%) with SARS-CoV suggesting its possible utilization of Angiotensin-Converting Enzyme 2 (ACE2) as a cell receptor [72]. However, several key residues required for the binding of the ACE2 receptor to the coronavirus receptor-binding domain displayed similarly between the receptor-binding domains of SARS-CoV and SARS-CoV-2. ...
... SARS-CoV-2 has a linear to initiate membrane fusion and enter human cells. Some critical amino acid residues (AA) in the RBD were different between SARS-CoV-2 and SARS-CoV (Gu et al. (2020 Sep 25); Tang et al. (2020); Lu et al. (2020); Andersen et al. (2020)). ...
... Since the outbreak of a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Wuhan, China, the disease has spread rapidly. 1 The World Health Organization declared coronavirus disease 2019 (COVID-19) a worldwide pandemic on March 11, 2020. 2 Digestive endoscopy procedures pose a risk of infection for healthcare workers due to the potential aerosol and fecal-oral transmission of microbial agents from patients. Pombo et al. reported that two-thirds of all nurses and half of all technicians were infected in the endoscopy unit during the COVID-19 pandemic. ...
... SARS-CoV-2 virus is detectable in saliva from infected individuals without symptoms or with mild symptoms [5], suggesting that a strategy of reducing the viral load in the oral cavity may reduce viral spread [6]. Several studies have shown the antiviral potential of mouthwashes, which decrease the infectivity of airborne viruses such as influenza and several coronaviruses, including SARS-CoV-2 [7]. In fact, the use of molecules such as cetylpyridinium chloride (CPC), commonly found in mouthwashes, has been postulated as a supplementary strategy to fight the transmission of viruses such as Influenza [8] or Herpesviruses [9], where the oral cavity plays an important role in spreading the virus. ...
... Besides coronavirus vaccination, several effective mouthrinses can be used to decrease the spread of coronavirus infection. Until now, 7 novel coronaviruses entailing 3 pandemic-causing viruses of SARS-CoV-2, MERS-CoV and the recently evolved coronavirus or SARS-CoV-2 [2][3][4][5]. These pandemic-causing viruses share indistinguishable nucleotide sequences [6]. ...
... Anelloviridae has also been detected in almost all parts of the human body, including the respiratory tract, and is recognized as the main component of the human viral flora [5,7,8]. While considered to be non-pathogenic, Anellovriridae might be associated with the occurrence of some disorders, including respiratory disorders in childhood [9][10][11][12][13]. Co-infection between TTV and common respiratory viruses have been reported, but the detailed composition of Anelloviridae, including the characterization of TTMV and TTMDV, has been poorly explored in children with respiratory virus infections [14,15]. ...