Na Zhang’s research while affiliated with China University of Geosciences (Beijing) and other places

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Publications (265)


The process of low temperature restrictive consecutively sub-cultured of microbial community LJ-7
Trends of Lignocellulose enzyme activity of low temperature LJ-7 in different generations. a Filter paper activity (FPA), b Endoglucanases (Cx), c Cellobiohydrolases (C1), dβ-glucosidases (CB), e Laccase (Lac), f Lignin peroxidase (LiP). The standard deviations for the mean of three replicates are shown as error bars
SEM results before and after straw degradation. a Rice straw before degradation (Magnification = 500×), b Rice straw after degradation (Magnification = 500×), c Rice straw before degradation (Magnification = 1000×) d Rice straw after degradation (Magnification = 1000×)
FTIR spectra of straw before and after degradation
Alpha diversity of LJ-7 at different generations of domestication. a Ace index, b Simpson index, c Shannon index and d Simpson index in OUT level at different culture stages

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Screening and community succession and functional prediction of high-efficiency degradation microbial communities for rice straw at low-temperature
  • Article
  • Publisher preview available

April 2025

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6 Reads

Extremophiles

Qiqi Fan

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Yu Zhang

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Jie Lian

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[...]

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Na Zhang

Traditional straw return relies on microbial degradation, but cold winters delay it, risking crops. Therefore, a microbial community adapted to rice straw degradation in cold regions was constructed by restrictive consecutively sub-cultured under low-temperature limitations. The capabilities of the microbial community, such as adaptability, stability, and degradation power, were evaluated by analyzing straw degradation features, Characterization experiments and lignocellulose enzyme activities across multiple generations. 16S amplicon sequencing was used to monitor the changes in its structure over generations. Metagenomic sequencing uses CAZy and KEGG to classify gene functions. The results showed that the highest degradation efficiencies and enzyme activities were observed in the E and F generations, dominated by Proteobacteria, Bacteroidetes, and Fungi The stable microbial community was designated as LJ-7. Metagenomic analysis showed that functional genes of LJ-7 were mainly concentrated in glycoside hydrolase (GHs) and glycosyl transferase (GTs) related genes which contained many fiber and lignin-degrading enzyme genes. It is speculated that microbial enzymes degrade straw by breaking down its complex structure into monosaccharides or metabolizing quinone compounds for energy. This experiment successfully screened a microbial community capable of degrading rice straw at low temperatures, thus offering novel research insights and pathways for straw degradation in cold conditions.

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Insights into the effects of phenolic acids on rice starch properties via noncovalent interactions

The development of advanced starch‐based materials is hindered by limited understanding of structure‐dependent phenolic acid (PA) binding mechanisms. This study employed a multi‐technique approach (Fourier‐transform infrared, x‐ray diffraction, rheology, molecular docking) to investigate 16 PAs with varying hydroxylation patterns and methoxy substitutions. Three distinct binding modes were identified: (1) Polyhydroxylated acids (e.g., gallic acid [GA]) formed bridge‐structured complexes with amylose helices (ΔH: 13.68 J/g; binding energy: −3.17 kcal/mol), increasing relative crystallinity (RC) by 18%–22% and reducing digestibility by 31%–35%; (2) methoxylated derivatives (e.g., syringic acid) bound helical cavities via hydrophobic interactions (ΔH: 9.21 J/g; binding energy: −2.86 kcal/mol), enhancing thermal stability without altering RC; (3) cinnamic acids preferentially interacted with amorphous regions through hydrogen‐bonding networks. These findings establish two fundamental principles for starch modification: (a) Phenolic architecture dictates supramolecular assembly pathways, and (b) binding site selection governs functional specificity. The acquired knowledge enables targeted design of starch materials for diabetic‐friendly foods (controlled digestibility) and biodegradable packaging (enhanced thermomechanical properties), advancing beyond empirical modification approaches. Future research should investigate dynamic binding behaviors during thermal processing and storage. Practical Application This study guides next‐gen PA–starch functional foods: (a) Selecting PAs with polyhydroxy/methoxy groups enables precise resistant starch design for rice‐based staples with tailored digestibility. (b) Heat‐stable polyhydroxy PA‐starch complexes (GA, HCA, 4HA) suit high‐temperature‐sterilized products like instant noodles, while phenolic acid derivatives (cinnamic acid) form amorphous networks for dual encapsulation of hydrophilic (B12) and lipophilic (D3) nutrients in low‐temperature pasty foods, overcoming fortification challenges.


Genistein improves depression-like behavior in rats by regulating intestinal flora and altering glutamate gene expression

March 2025

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5 Reads

Current Research in Food Science

Depression is a mental disorder, and genistein is known to have antidepressant effects, but its mechanism of action is still unclear. Here, the mechanism of genistein improving depression based on gut microbiota was explored using classic behavioral indicators of depression combined with genomic technology. The behavioral evaluation showed that rats gavaged with 20–40 mg/kg genistein showed an increase in body weight, glucose preference, absenteeism score, body temperature, and 5-hydroxytryptamine (5-HT) content, while a decrease in adrenocorticotropic hormone (ACTH) and corticosterone (CORT) content compared to the depression rat model group, but there was no significant difference compared to the positive control (fluoxetine). The results of high-throughput sequencing showed that genistein increased the relative abundance of Firmicutes and Actinobacteriota and decreased the relative abundance of Bacteroidota at the phylum level. At the genus level, the abundance of Bifidobacterium, a short-chain fatty acid producing bacterium, was increased. Furthermore, metagenome results revealed that the antidepressant effect of genistein can be achieved by promoting glutamate metabolism, increasing glutamic acid decarboxylase (GAD) expression levels, promoting γ-aminobutyric acid (GABA) synthesis, and indirectly increasing 5-HT levels.




Citations (31)


... Compared with the AD group, the increase in volatile compound content observed in the HAD group can be attributed to the high-temperature environment facilitating reactions between carbonyl compounds (reducing sugars) and amino compounds (amino acids and proteins), known as the Maillard reaction or carbonyl-amino reaction [19]. This In a plasma, certain particles and groups can undergo chemical reactions with surrounding substances under appropriate conditions, resulting in the formation of new material structures that alter the physical and chemical properties of the original substances. ...

Reference:

Study on the Effects of High-Voltage Discharge Plasma Drying on the Volatile Organic Compounds and Texture Characteristics of Oat Grass
Effects of phytic acid from soybean meal on Maillard reaction and antioxidant properties of products
  • Citing Article
  • September 2024

Food Chemistry

... HHP processing is an environmentally sustainable, non-thermal technique that utilizes isostatic pressures between 100 and 1000 MPa, with or without added heat (Allai et al. 2023). HHP is a batch system that is extensively employed in the food industry to enhance the microbial safety, nutritional content, and functional properties of food products by utilizing water as a medium to transmit pressure (Wang, Ma, et al. 2024). The minimal impact on bioactive compounds is a significant advantage of HHP, which is a result of the low or moderate temperatures involved. ...

Effect of high hydrostatic pressure treatment on food composition and applications in food industry: A review
  • Citing Article
  • August 2024

Food Research International

... SCFAs stimulate L-cells to secrete Glucagon-like peptide-1, which promotes insulin secretion and alleviates T2DM. Additionally, SCFAs reduce the levels of inflammatory markers through intestinal epithelial cells, contributing to lower blood glucose levels [24]. ...

The Role and Mechanism of Probiotics Supplementation in Blood Glucose Regulation: A Review

... On the other hand, long-chain fatty acid derivatives like palmitic acid and stearic acid augment the hydrophobicity of starch, potentially reducing its water solubility but enhancing its solubility in non-polar solvents. Long-chain fatty acid-modified starches often exhibit improved thermal stability and mechanical properties, making them advantageous for high-temperature processing and various applications [153][154][155]. ...

Effect of different chain-length fatty acids on the retrogradation properties of rice starch
  • Citing Article
  • August 2024

Food Chemistry

... In particular, the FRAP assay, which measures the ability of a sample to reduce ferric ions to ferrous ions, may show elevated antioxidant values due to the presence of bioactive peptides containing a high proportion of amino acids such as histidine, cysteine, and methionine, which facilitate iron reduction. These amino acids are known for their ability to interact with metal ions and exhibit reducing activity [35]. The potential predominance of such amino acids in the peptide mixture could enhance the observed antioxidant activity in the FRAP assay compared to other methods, which focus on different mechanisms such as scavenging free radicals or measuring overall oxygen radical absorbance capacity. ...

Exploring the Potential of Plant-Derived Metal ion Binding Peptides: Preparation, Structure-Activity Relationship, and Biological Activities
  • Citing Article
  • July 2024

Trends in Food Science & Technology

... Their method effectively solves the stability problem when there is simultaneous quantization of the control inputs and state measurements. Data quantization introduces quantization errors that affect the accuracy of the control system [32][33][34]. In high-speed train speed-tracking control, finding the optimal way to effectively deal with quantization errors in order to ensure the performance of the control system is an urgent matter. ...

Quantization-Based Adaptive Fuzzy Consensus for Multiagent Systems Under Sensor Deception Attacks: A Novel Compensation Mechanism
  • Citing Article
  • October 2024

IEEE Transactions on Cybernetics

... In reality, the interaction between them usually involves a combination of multiple types of forces. Zhang, Yang et al. (2024) found that coupling with maltose extended the otherwise compact structure of soybean isolate proteins, increased molecular flexibility, and led to increased exposure of hydrophobic residues and charged charges within them. Their aggregates could form a thicker interfacial layer at the oil-water interface, providing a better three-dimensional barrier and enhancing the spatial repulsion between oil droplets Nooshkam et al., 2023). ...

High freeze-thaw stability of Pickering emulsion stabilized by SPI-maltose particles and its effect on frozen dough
  • Citing Article
  • July 2024

International Journal of Biological Macromolecules

... Using a modified method of Chen et al. (2024), aqueous sample solutions were separately prepared and used to constitute the reaction mixture in a 96-well plate model, comprising of sample solution (or various concentrations of rice flour solutions) and 0.06 % I 2 /KI reagent solution in a 1:1 v/v ratio. Absorbance was measured at 600 nm using a spectrophotometer. ...

Structural, physicochemical, and digestive properties of enzymatic debranched rice starch modified by phenolic compounds with varying structures
  • Citing Article
  • June 2024

International Journal of Biological Macromolecules

... Enzymatic hydrolysis enhances protein digestibility by breaking down high-molecularweight proteins into smaller peptides and amino acids, making them more accessible to digestive enzymes. A study demonstrated that limited hydrolysis using papain significantly reduced the molecular weight of a soy protein isolate and corn zein mixture while increasing the proportion of random coil structures [145]. These structural modifications facilitated protein breakdown, resulting in a remarkable improvement in in vitro digestibility. ...

Tailoring soy protein/corn zein mixture by limited enzymatic hydrolysis to improve digestibility and functionality

Food Chemistry X

... Studies have shown that piperine can enhance the bioavailability of certain drugs and exert regulatory effects on the nervous and immune systems [13]. In addition, the fruit of PN contains volatile oil components, such as terpenes and aromatic compounds, which primarily contribute to its characteristic aroma [14]. It has been shown that certain polyunsaturated fatty acids in PN, such as oleic acid and linoleic acid, offer significant cardiovascular health benefits [15]. ...

Preparation, pungency and bioactivity transduction of piperine from black pepper (Piper nigrum L.): A comprehensive review
  • Citing Article
  • June 2024

Food Chemistry