N N Kevorkov’s research while affiliated with Institute of Ecology and Genetics of Microorganisms and other places

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Publications (46)


EFIT1996
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January 2018

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22 Reads

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Kevorkov N.

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Tokmakova O.
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Черешнева М.В., Шилов Ю.И., Черешнев В.А., Кеворков Н.Н., Осипенко А.В., Гаврилова Т.В. Иммунокоррекция в комплексном лечении больных с воспалительными заболеваниями роговой и сосудистой оболочек глаз. Екатеринбург: УрО РАН, 2004. 253 c. ISBN: 5-7691-1390-1.

January 2004

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19 Reads

Collective monograph in Russian. Approaches to pathogenetic immune correction in inflammatory diseases of the corneal and vascular membranes of the eye including the use of immunomodulators on the base of clinical and experimental investigations were discussed.



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[Lymphotropic immune correction with myelopid in the postoperative period in patients with cancer of the colon and rectum]

February 2002

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23 Reads

Grekov s Bulletin of Surgery

An analysis of surgical treatment of 142 patients with cancer of the colon and rectum included 32 emergency cases and 110 scheduled operations. Standard surgical methods only were used in 94 patients, the lymphotropic immunocorrection with myelopidum in the postoperative period was made in 48 patients. The use of lymphotropic immunocorrection was shown to considerably improve the immunological parameters and to decrease postoperative lethality.


Industrial environment and the immune system

March 2001

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36 Reads

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2 Citations

Clinical and Applied Immunology Reviews

Major issues of the new scientific field of ecological immunology are considered. The principles of carrying out the immunotoxicological examinations are studied by the example of long-term observa-tions from the ecological–immunological monitoring of adults and children from the West Ural, and also by the experiments (field tests) with animals. The concept of secondary ecologically-dependent immune deficient state is formulated, and the means for immune correction, including prevention, therapy and rehabilitation of the immune system alterations, are addressed.



Черешнева М.В., Шилов Ю.И., Баданина О.Н., Черешнев В.А., Кеворков Н.Н., Пономарева Т.Б., Шилов С.Ю. Иммунокоррекция при ранении глаза. Екатеринбург: УрО РАН, 2001. 148 с. ISBN 5-7631-1101-1.

January 2001

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17 Reads

M. V. Chereshneva, Yu. I. Shilov, O. N. Badanina, V.A. Chereshnev, N.N. Kevorkov, T.B. Ponomareva, S.Yu. Shilov Immune Correction in Eye Injury [in Russian], Ekaterinburg, 2001. 148 pp. Collective monograph in Russian. Experimental and clinical data on immune disorders and immunomodulatory therapy under eye injury were discussed.



A novel method for CD-positive cells identification

January 1998

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8 Reads

The FASEB Journal

The aim of this work was the development of a method for direct identification of CD molecules expressed on the human limphocyte surface. The method is based on the use of biotinylated monoclonal antibodies to CD molecules and detecting reagents which were synthesized by covalent binding of Streptavidin with carbon suspen- zoid particles (Streptavidin-C conjugate) as labels. High optical density of these labels gives the possibility to register them in the sites of antibody-CD complex formation using light microscopy. The lymphocytes were separated from human venous blood and incubated with mouse anti-human CD8 biotinylated antibodies. After fixation the smears were covered with Streptavidin-C conjugate in different concentrations for 30 minutes. Then the slides were dyed with hematoxylin. CD8+ cells were calculated using light microscopy. The significant correlation was obtained when comparing light microscopy results with the ones of immunofluorescent detection of CD+ lymphocytes. Apparently developed method can be used to detect other CD-positive cells depending on a specificity of utilized biotinylated antibodies. Stability, visualability, economic feasibility and simplicity in application of the used detecting reagents effectively distinguish them from available enzymatic and fluorescent labels and make it possible to reject the expensive recording equipment.



Citations (7)


... In all the examples, sex hormones are found to influence parasite proliferation , macrophage function, inflammation or reproductive and/or exploratory behavior of the parasitized host (Larralde et al., 1995; Zhang et al., 2001; Roberts et al., 2001). Sex steroids also impinge upon the immunological network at both cellular and cytokine levels (Sthoeger et al., 1988; Grossman et al., 1991; Bojalil et al., 1993; Terrazas et al., 1994; Shirshev et al., 1995; Roberts et al., 2001; Morale et al., 2001; Morales-Montor et al., 2001c; Rohleder et al., 2002; Zang et al., 2002). These interactions suggest the potential application of sex hormones in prevention and treatment of parasitic diseases (Beckage, 1991; Zuk and McKean, 1996; Klein, 2000). ...

Reference:

Castration and pregnancy of rural pigs significantly increase the prevalence of naturally acquired Taenia solium cysticercosis
Influence of female sex steroids on the capacity of splenocytes to form an adoptive immune response. Role of prostaglandin F2α in the mechanisms of hormonal immunoregulation

Neuroscience and Behavioral Physiology

... From these data it was concluded that industrial factors (crude oil and radiation exposure) can lead to the development of secondary T-cell and combined immune deficiency. The second component of immune monitoring involved the examination of children residing in ecologically unfavorable regions [11] [12] [13]. This observation has been ongoing for 10 years. ...

Influence of unfavourable environment on the interrelations between age and immune system in children

... в условиях экспериментального гипотиреоза [8], а в последующем выявлено нарушение развития клеток иммунной системы у мышей с дефектами генов, кодирующих разные изоформы тиреоидных рецепторов [3,7]. Отечественными авторами изучены механизмы влияния тиреоидных гормонов на иммунный ответ, кооперацию Т-и В-лимфоцитов, формирование основных субпопуляций Т-лимфоцитов, процессы антигеннезависимого созревания Т-и В-клеток в условиях экспериментального тиреотоксикоза разной степени тяжести [1,4,5]. Показано, что введение трийодтиронина усиливает экспорт Т-лимфоцитов из тимуса в лимфатические узлы и способствует перераспределению ранних тимических эмигрантов между лимфатическими узлами и селезенкой [12]. ...

Effects of thyroxine on T and B cell development

... В экспериментальной модели блокады β-адренорецепторов при остром 24-часовом иммобилизационном стрессе в сочетании с дозированной кровопотерей при системном иммунном ответе, перед проведением основного эксперимента исследована зависимость доза-эффект развития при системном иммунном ответе реакции ГЗТ и антителопродукции при внутрибрюшинной сенсибилизации крыс разными дозами эритроцитов барана (ЭБ). Хотя этот аспект детально изучен для разных линий мышей [6, 7], у крыс подобная зависимость исследована недостаточно. Установлено, что увеличение дозы антигена приводит к повышению числа АОК в селезенке и уровня антител в плазме крови (рис. ...

Characteristics of T-suppressors responsible for cellular and humoral immune response competition in the spleen
  • Citing Article
  • March 1993

Bulletin of Experimental Biology and Medicine

... Elevated levels of glucagon may also contribute to dysregulation of innate immune cells. Reduced bacterial killing and adaptive immune activation were seen after exposure to high concentrations of glucagon, as evidenced by an impaired respiratory burst, reduced chemotaxis and neutrophil accumulation [173], a shift in gene expression of pro-and anti-inflammatory cytokines in monocytes [174], and reduced numbers and activity of NK-cells [175][176][177]. However, conflicting reports show increased superoxide production in neutrophils and improved cell survival after blockade of the glucagon receptor [178]. ...

[Immunomodulating effects of glucagon]
  • Citing Article
  • September 1987

Problemy E̊ndokrinologii

... В экспериментальной модели блокады β-адренорецепторов при остром 24-часовом иммобилизационном стрессе в сочетании с дозированной кровопотерей при системном иммунном ответе, перед проведением основного эксперимента исследована зависимость доза-эффект развития при системном иммунном ответе реакции ГЗТ и антителопродукции при внутрибрюшинной сенсибилизации крыс разными дозами эритроцитов барана (ЭБ). Хотя этот аспект детально изучен для разных линий мышей [6, 7], у крыс подобная зависимость исследована недостаточно. Установлено, что увеличение дозы антигена приводит к повышению числа АОК в селезенке и уровня антител в плазме крови (рис. ...

[Genetic and phenotypic aspects of the interrelationship between cellular and humoral immune response to sheep erythrocytes in mice]
  • Citing Article
  • April 1993

Bulletin of Experimental Biology and Medicine