N I Novikova’s research while affiliated with Russian Academy of Sciences and other places

The article reveals that a decrease in the background production of reactive oxygen species (ROS) in the peritoneal neutrophils of mice after a short-term (40 minutes) stay in hypomagnetic conditions (residual field  10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, is not accompanied by a violation of chemiluminescent response to respiratory burst activators: formylated peptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester of phorbol-12-meristat-13-acetate (PMA). These results were obtained by activated chemiluminescence using lucigenin or luminol and various combinations of ROS production activators (PMA and/or fMLF). In contrast, the action of combined parallel constant (induction 60 μT) and alternating (amplitude range 60-180 nT, frequency 49.5 Hz) magnetic fields (CMF) leads to a decrease in the chemiluminescent response to these activators. These data indicate different sources of ROS that respond to certain modes of CMF and hypomagnetic field in neutrophils. The conducted research and the previously obtained results enable to exclude the systems that control the respiratory burst in neutrophils from the main targets and acceptors that respond to short-term deprivation of the magnetic field.

It has been shown that various chemical agents (ethylenediaminetetraacetic acid, zinc sulphate, ethyl alcohol, and rotenone) have different effects on preactivation (priming) of neutrophils that develop under weak combined collinear static and alternating magnetic fields (combined magnetic fields: a static field of 42 μT and an alternating field of 0.86 μT with the sum of frequencies 1.0, 4.4 and 16.5 Hz). Low concentrations (0.05%) of ethylenediaminetetraacetic acid decrease the intensity of luminol dependent chemilumi-nescence of neutrophils in response to an activator of the respiratory burst the peptide N-formyl-Met-Leu-Phe under the action of combined magnetic fields to a lesser extent than in the control. In contrast, ethyl alcohol (0.45%) and zinc sulphate (0.1 mM) have a greater effect on this process under the action of combined magnetic fields. Rotenone (1 μM) has a weak effect on neutrophil chemiluminescence both under the action of combined magnetic fields and in the control.