Morteza Mahmoudi’s research while affiliated with Michigan State University and other places

... AI and advanced analytics offer a wide range of solutions to handle such high-dimensional data, providing sophisticated approaches to handle missing data, mitigating batch effects, and providing customizable solutions with various constraints through optimization algorithms [84]. AI methods can simulate multiple protein corona modulations to enhance deep proteome profiling of nanoparticles [85]. ...

Reference:

Artificial Intelligence Applications in Cardio-Oncology: A Comprehensive Review

... However, the treated sample exhibited a significant signal corresponding to the small molecule after 60 min of separation time (Fig. 3b), validating our hypothesis that small molecules interact with plasma proteins, causing the observed variation in the protein corona on the NPs' surface. Furthermore, the process of recovering intact proteins from the surfaces of NPs primarily collects proteins from the outer layer of the protein corona 85 , as the inner layer is tightly bound to the NP surfaces through various physical and chemical forces 86 . This observation further confirms that PtdChos interacts with plasma proteins rather than directly with the NP surfaces, leading to the formation of its unique protein corona composition. ...

Reference:

Small molecule modulation of protein corona for deep plasma proteome profiling

... Protein elution from the surface of NPs and purification were conducted based on procedures illustrated in our recent publications 85,100 . The protein corona-coated NPs (with/without PtdChos) were separately treated in a 0.4% (w/v) SDS solution at 60°C for 1.5 h with continuous agitation to release the protein corona from the NP surface. ...

Reference:

Small molecule modulation of protein corona for deep plasma proteome profiling

... This corona can significantly alter nanoparticle properties, including surface charge, hydrodynamic diameter, colloidal stability, and cellular interactions, ultimately affecting biodistribution, oxidative stress responses, and toxicity profiles [60,61]. Recent studies have shown that the corona may either attenuate or exacerbate reactive oxygen species (ROS) production, depending on its protein composition and conformational dynamics [62]. For example, in zebrafish liver cells, protein corona formation was found to influence NP-induced oxidative stress and disrupt glycolipid metabolism [63]. ...

Reference:

Exploring Oxidative Stress Mechanisms of Nanoparticles Using Zebrafish (Danio rerio): Toxicological and Pharmaceutical Insights

... It is noteworthy that the superior performance of PtdChos alone compared to Molecular Sauce 1 could be attributed to interactions between the small molecules in the mixture, which may have lowered the effective concentration of PtdChos (for example, the interactions between PtdChos and triglycerides) 68,69 . Mass spectrometry workflow and the type of data analysis have a critical influence on proteomics outcomes in general 9,70-73 , as well as in the specific field of protein corona research 56,57,74 . For instance, our recent study demonstrated that identical corona-coated polystyrene NPs analyzed by different mass spectrometry centers resulted in a wide range of quantified proteins, varying from 235 to 1430 (5.1 fold increase as compared to plasma alone) 56 . ...

Reference:

Small molecule modulation of protein corona for deep plasma proteome profiling

... This technology has been widely applied in tissue engineering due to its ability to precisely control the deposition of reagents. Leveraging these advantages, numerous cardiovascular devices have been bioprinted to achieve structural complexity and enhanced functionality [8,[73][74][75]. Strategies to bioprint cardiac patches can vary based on the bioprinting modalities as well as bioinks used for printing. ...

Reference:

3D Bioprinted Cardiac Patch Devices for Regenerative Therapies

... Diabetic wounds rank among the most prevalent complications associated with diabetes, causing severe pain and high mortality in patients [1][2][3]. Growth factor-based treatment strategies have shown effective therapeutic effects for diabetic wounds, mainly relying on the capability to regulate cellular proliferation, stimulate angiogenesis, and repair tissues [4][5][6][7]. These growth factors include platelet-derived growth factor (PDGF), fibroblast growth factor-7 (FGF-7), vascular endothelial growth factor (VEGF), etc. ...

Reference:

Biomimetic Triplet Messenger RNA Formulation in MXene Hydrogel Microneedles for Wound Healing

... 13 Consequently, there is an urgent need to develop a standardized protocol for protein corona analysis to reduce data heterogeneity and facilitate comparability across studies. 13,14 Our previous research indicates that major disparities in the characterization of the protein corona composition are primarily due to variations in sample preparation protocols, LC-MS workflows, and data processing. 13 We also revealed that harmonizing database search and data processing can significantly reduce the observed heterogeneity among core facilities. ...

Reference:

Standardizing Protein Corona Characterization in Nanomedicine: A Multicenter Study to Enhance Reproducibility and Data Homogeneity

... The protein corona was prepared on the polystyrene NPs (PSNPs) according to the used procedure in recent studies. [12,29] It is noteworthy that we used PSNPs due to our extensive experience in optimizing the parameters involved in the formation of a pure protein corona, ensuring highly accurate and reproducible MS results. Full details on the PSNP optimization and characterization for protein corona formation are available in our recent publications. ...

Reference:

Deciphering nanoparticle protein corona by capillary isoelectric focusing-mass spectrometry-based top-down proteomics

... hard-corona-bearing NPs can be isolated, and their corona protein composition can be determined [10,11]. Along with the proteins of the hard corona, which are tightly bound to the NPs' surface, there are proteins with low affinity to the surface, which form an extremely variable layer-the "soft corona", the composition of which easily changes under the influence of environmental factors [9]. ...

Reference:

The Photomodification Method Allows for Determining the Composition of the Full and Soft Protein Corona on the Lipid Surface of Composite Nanoparticles