Morteza Daliri's research while affiliated with National Institute of Genetic Engineering and Biotechnology and other places
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Publications (34)
Despite the widespread central nervous system injuries, treatment of these disorders is still an issue of concern due to the complexities. Natural recovery in these patients is rarely observed, which calls for developing new methods that address these problems. In this study, natural polymers of polyhydroxybutyrate (PHB) and gelatin were electrospu...
Lipopolysaccharide (LPS) derived from gram negative bacteria cell wall is known to cause ruminal acidosis and/or infectious diseases such as metritis and mastitis which has a significant negative impact on the reproductive performance. This study aimed to investigate the effect of LPS on oocyte maturation and subsequent development in vitro. Ovine...
The reconstruction of the nerve tissue engineering scaffold is always of particular interest due to the inability to recover and repair neural tissues after being damaged by diseases or physical injuries. The primary purpose of this study was obtaining a model used to predict the diameter of the fibers of electrospun polyhydroxybutyrate (PHB) scaff...
In this study Primary cell lines were established from the culture of explants of caudal fin of Persian sturgeon, Acipenser persicus and the effect of media, temperature and FBS on fin explants culture in this fish was investigated. The margin of the caudal fin from a two year old specimen was aseptically cut, washed three times with PBS containing...
In this study Primary cell lines were established from the culture of explants of caudal fin of Persian sturgeon, Acipenser persicus and the effect of media, temperature and FBS on fin explants culture in this fish was investigated. The margin of the caudal fin from a two year old specimen was aseptically cut, washed three times with PBS containing...
In this study Primary cell lines were established from the culture of explants of caudal fin of Persian sturgeon, Acipenser persicus and the effect of media, temperature and FBS on fin explants culture in this fish was investigated. The margin of the caudal fin from a two year old specimen was aseptically cut, washed three times with PBS containing...
Micro RNAs (miRNAs) are a novel class of non-coding regulatory RNA molecules that contribute to post-transcriptional gene regulation. Recent studies have demonstrated that specific miRNAs such as miR-150, miR-154, and miR-451 have key roles in erythropoiesis. To date, stimulatory cytokines are considered as unique effectors for in vitro differentia...
Objective(s): Erythropoiesis is regulated by some extrinsic and intrinsic factors as microRNAs (miRNAs). miRNAs are endogenously small non-coding regulatory RNAs which play vital roles in the variety of cellular fate, critical processes; growth, apoptosis, metabolism, survival of the cells and specially differentiation. Several miRNAs such as miR-1...
Chitinases have received attention because of their wide applications in the medicine, biotechnology, agriculture, waste management and industrial applications such as food quality enhancer and biopesticide. Excessive use of insecticides has led to several problems related to pollution and environmental degradation. In this study, isolation and ide...
Mesenchyma stem cells (MSCs) are multipotent cells. They are capable of transforming into several mesoderm lineages. They can be separated from different tissues, such as bone marrow, umbilical cord blood, peripheral blood, and adipose tissue. Stem cell based therapies for the repair and regeneration of individual tissues and organs suggest a parad...
Recent evidence proposes that cells with the properties of mesenchymal stem cells (MSCs) can be extracted from adult peripheral tissues, including muscle, dermis and adipose tissue. Adipose tissue is ubiquitous and easily obtainable in large quantities. We separated Adipose derived stem cells (ADSCs) from part of subcutaneous adipose tissue from ra...
Although alumina scaffold with biodegradable polymer coating can overcome the limitations of conventional ceramic bone substitutes, the bioactivity potential of these scaffolds needs to be enhanced. In this study, the macroporous alumina scaffolds with the defined pore-channel interconnectivity were successfully prepared by foam replication method....
The aim of this study was to investigate the thermal behavior of Mg-doped calcium phosphate compounds. Nanocrystalline HA and -TCP mixtures containing different magnesium contents were synthesized via an alkoxide sol gel method. The ratio of (Ca+Mg)/P was kept constant at 1.67, and the Mg content ranged between 0 and 3 mol%. The influence of magnes...
Alumina is a bioceramic with improved mechanical properties in
comparison to hydroxyapatite. It is also an inert biomaterial in the
human body environment of good biocompatible characteristics. Silane
coupling agents are capable to form a durable bond between organic and
inorganic materials. However, formation of hydroxyl functional groups
may enha...
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indi...
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent molecular mass of 54 kDa. It indi...
The best solvent type and ratio for grafting of poly-n-isopropylacrylamide (PNIPAAm) on the surface of polystyrene is obtained under ultraviolet radiation. In this study, the effects of solvents, such as water, methanol, and their combinations, under ultraviolet radiation were investigated successfully.
Attenuated total reflection Fourier transform...
Chitinases have the ability of chitin digestion that constitutes a main compound of the cell wall in many of
the phytopathogens such as fungi. In the following investigation, a novel chitinase with antifungal activity
was characterized from a native Serratia marcescens B4A. Partially purified enzyme had an apparent
molecular mass of 54 kDa. It indi...
marcescens B4A was optimized following Taguchi’s
array methods. Twenty-three bacterial isolates were
screened from shrimp culture ponds in the South of
Iran. A chitinase-producing bacterium was isolated
based on it’s ability to utilize chitin as the sole carbon
source. The isolate designated as B4A, was identified
as Serratia marcescens based on it...
BACKGROUND: Making (nano)composite structures is one of the efficient approaches for strengthening hydrogels extended in recent years. The present paper deals with the synthesis and properties of novel nanocomposite hydrogels based on 2-acrylamido-2-methylpropane-1-sulfonic acid (AMPS). Initially, a bio-modified clay, chitosan-intercalated montmori...
A r c h i v e o f S I D Iranian Polymer Journal 18 (4), 2009, 297-306 microtubules scaffold; solid-liquid phase separation; PLLA; P19 mouse embryonic cell. I n this study, nerve guidance material was designed and fabricated from crystalline poly(L-lactic acid) (PLLA) with microtubules orientation structure by using thermal-induced phase separation...
The porous calcium phosphate scaffolds with defined pore-channel interconnectivity were successfully prepared via negative
replica method. The internal channel architecture was achieved using a water-insoluble polyurethane sponge. The PU sponge
was first filled with aqueous slurry of biphasic calcium phosphate material containing pore forming agent...
In this study, samples of poly(dimethylsiloxane) (PDMS) with different crosslink densities were prepared in order to produce samples with different crosslink density and, subsequently, different molecular mobilities. Crosslink density and chain mobility changes in PDMS networks were determined using differential scanning calorimetery and dynamic me...
This work has been undertaken to study the cell behavior of L929 fibroblasts on the laser irradiated polyethylene terephthalate (PET) surface. To modify the surface properties of the PET, CO2 pulsed laser at the wavelength of 9.25 microm and KrF excimer laser at 248 nm with various number of pulses were used. Laser irradiation caused some changes i...
This work has been undertaken to study the cell behavior of L929 fibroblasts on the laser irradiated polyethylene terephthalate (PET) surface. To modify the surface properties of the PET, CO2 pulsed laser at the wavelength of 9.25 μm and KrF excimer laser at 248 nm with various number of pulses were used. Laser irradiation caused some changes in th...
Attempts have been made to evaluate the degree of bioadhesion and biocompatibility of a synthesized urethane prepolymer with specially tailored microstructure. Wetting behaviour and extent of interfacial adhesion of the prepared prepolymer towards biological substrates were examined by in vitro methods. The former was carried out by measuring the c...
Citations
... It was also observed that the LPS increased the proliferation rate in different cell models including epithelial cells from other species through inducing oxidative stress (Chanrot et al., 2017). Recent findings concluded that in vitro exposer of oocytes with LPS affects the oocyte maturation and subsequently embryonic developmental competence through triggering the oxidative stress, pro-apoptotic effects and immune response (Zhao et al., 2017;Rincon et al., 2019;Heydari et al., 2020;Rasekhi et al., 2020). ...
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... An efficient model could reduce the number of laboratory analyses, time consumption, and related costs using prior information and intelligent prediction schemes [17]. Among the well-established computational techniques, the artificial neural network (ANN) has become a promising approach in the structure-property analysis of polymers [18,[26][27][28][29]. Different mechanical and thermal properties such as wear resistance [30], elastic modulus [24], thermal stability [26], and crystallinity [26] were predicted based on the ANN technique. ...
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... Protease activity was determined by the degradation of casein, according to Rais et al. [106]. Chitinase activity was measured according to the method described by Zarei et al. [107], with colloidal chitin as the substrate. The lytic enzyme activity was calculated following the formula described by Rais et al. [106]. ...
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... The S. marcescens produces red colonies in this medium that facilitates their isolation. After 72 h, suspected red colonies grown in plates containing samples of cultured water were subcultured in nutrient agar medium (Zarei et al., 2012). ...
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... 21,23,24,41,42 Due to the biological action of magnesium (Mg), CaP compounds partially enriched with bivalent Mg ions have attracted the interest of many scientists. 43,44 Mg is one of the most important trace ions in physiological apatite, which stimulates osteoblast proliferation and influences bone resorption. 43,44 Its depletion disturbs all phases of skeletal metabolism, resulting in low bone growth and reduced osteoblastic and osteoclastic activities. ...
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... Several in vitro hepatocyte differentiation protocols were developed based on the administration of hepatic inducers, either in a stepwise manner or as a single complex mixture to cultured cells (Iacono et al., 2013;Khoshdel et al., 2012;Wasel et al., 2013). ...
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... Adipose tissue has been of interest due to containing large amounts of SCs compared to bone marrow and their ability to bind to native and non-native hosts [132]. A noteworthy point is the easy access to large volumes of fat during the liposuction process [133]. ASCs and bone marrow stromal cells are similar in terms of gene expression and differentiation; however, ASCs have a higher potential to self-replicate [134]. ...
... Recent studies have suggested that miRNAs are closely involved in the regulation of autophagy by regulating the expression of key proteins [26]. MiR-451 participates in various physiological and pathological processes, including nervous system development, epithelial cell polarity, embryonic development, and hematopoietic system differentiation [27][28][29]. It has recently been identified as a potential therapeutic target for cancers [30]. ...
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... Since there are a lot of methodologies to measure in vitro antifungal activity, varying results have been obtained. In the antifungal tests generally test fungi were inseminated in the center of the Petri plates containing PDA and when the diameter of the colony was almost 2 cm, enzyme impregnated with sterile blank paper discs locates the center of the plates [5]. Thakur et al., [6] purified and characterized extracellular chitinase from B. cereus NK91 and then tested the partially purified enzyme for its antifungal activity against some phytopathogens. ...
... In contrast, Kouhkan et al. did not observe an effect of miR-16 on HBG expression in CD133 + stem cells [75]. Furthermore, miR-26b was shown to directly interact with the 3′UTR of MYB and induce HbF in both erythroid progenitors and K562 cells [56]. ...
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