Mohammed Chado Isah’s scientific contributions

This work reports the effect of some serum and gill metabolic waste (creatinine, urea and total bilirubin) of catfish fed blood meal and moringa leaf as a supplement diet. Among the other five diets, fish meal was altered from 7%, 5%, 2%, 0%, and 0% of its replacement with blood meal. The control fed had no blood meal and moringa leaf. 0%, 2%, 5%, 7%, 10%, 10%. The fishes were dissected after 8 weeks and the serum and gills were collected for the analysis of creatinine, urea and total bilirubin of the serum and gills using spectrum photometric method. The physic-chemical of each diet for (temperature, pH, dissolved oxygen and conductivity) was monitored. Creatinine shows significant difference (P<0.005) in the fishes from (0.493±0.007mg/dl) to (0.380± 0.006mg/dl). Total bilirubin shows no significant difference in gill from (0.0.413 ± 0.009mg/dl) to (0.643 ± 0.013mg/dl). While serum shows significant differences from (6.280 ± 0.006mg/dl) to (5.257 ± 0.009mg/dl). Urea shows a significant difference in both serum and gills (6.280 ± 62.800 ± 0.058mg/dl) to (46.500 ± 0.115mg/dl). These metabolic waste activities in the fish's serum and gills demonstrate the impact of 10% blood meal and moringa leaves on fish physiology. The fish were fed with the experiment diets in triplicates for 8 weeks, after which fish serum and gills from each treatment were collected to analyse creatinine, urea and total bilirubin with the spectrophotometric method.