Mohammad KaramiNejadRanjbar's research while affiliated with Georg-August-Universität Göttingen and other places

Publications (6)

Article
The use of a site-specific homing-based gene drive for insect pest control has long been discussed, but the easy design of such systems has become possible only with the recent establishment of CRISPR/Cas9 technology. In this respect, novel targets for insect pest management are provided by new discoveries regarding sex determination. Here, we pres...
Article
Even in times of advanced site-specific genome editing tools, the improvement of DNA transposases is still on high demand in the field of transgenesis: especially in emerging model systems where evaluated integrase landing sites have not yet been created and more importantly in non-model organisms such as agricultural pests and disease vectors, in...
Article
Full-text available
The Sterile Insect Technique (SIT) is an accepted species-specific genetic control approach that acts as an insect birth control measure, which can be improved by biotechnological engineering to facilitate its use and widen its applicability. First transgenic insects carrying a single killing system have already been released in small scale trials....

Citations

... The most commonly cited mode of resistance is mutations in the target cut sites "resistance alleles," which prevent further recognition by the Cas9/sgRNA complex and therefore targeting by the drive. If resistance alleles do not severely disrupt the gene's coding potential (e.g., synonymous mutations, or small in-frame deletions), they may be rapidly selected for in a drive carrying population as the fitness differential between the resistance mutation and the drive is expected to be large (Champer et al., 2017;Carrami et al., 2018). Several strategies can mitigate this, including restricting expression of the gene drive to the germline (Hammond et al., 2021) targeting highly conserved gene sites, hoping that this indicates low tolerance for mutated alleles. ...
... After sequence analysis to confirm the presence of SNPs in the Dll319 CRE, plasmid DNA was amplified, using a Midiprep kit (Qiagen). The piggyGUE Dll319 CRE plasmid was diluted to 1 μg/μL and mixed in a 1:1 ratio with a hyperactive piggyBac transposase plasmid (35). Embryos (n = 550) were collected from B. anynana butterflies reared at 27°C and injected ∼1 h after egg laying with the plasmid solution and a small amount of food dye, using a glass injection needle and nitrogen gas pressure. ...