Mitsuyasu Ushijima's research while affiliated with Wakunaga Pharmaceutical Co., Ltd. and other places

Publications (37)

Article
Aged garlic extract (AGE) has been shown to improve peripheral circulatory disturbances in both clinical trials and experimental animal models. To investigate the effect of S-1-propenylcysteine (S1PC), a characteristic sulfur compound in AGE, on cold-induced reduction in tail blood flow of rat, Wistar rats were individually placed in a restraint ca...
Article
Our previous study has shown that a single dose of S-1-propenylcysteine (S1PC) exerted an antihypertensive effect in spontaneously hypertensive rats (SHR), while its mode of action remained to be further investigated. The aim of this study was to explore the potential mechanism of the antihypertensive effect of S1PC in SHR using a liquid chromatogr...
Article
Full-text available
Background Plants of Allium spp., including garlic (A. sativum) and onions (A. cepa), are known to be oxidatively toxic to canine erythrocytes resulting in Heinz body hemolytic anemia in dogs. In humans, these plants have been used as medicinal agents for multiple diseases since ancient times. Especially, fresh garlic extracted over a prolonged per...
Article
Full-text available
The degradation of target proteins by small molecules utilizing the cellular proteolytic system is featured as a treatment strategy of several diseases. We found that S-1-propenylcysteine (S1PC) among several cysteine derivatives in aged garlic extract inhibited TLR-mediated IL-6 production by inducing the degradation of adaptor protein MyD88. We s...
Article
Objectives: This study was designed to investigate the antihypertensive effect of S-1-propenylcysteine, a characteristic sulfur compound in aged garlic extract, using a hypertensive rat model. Methods: The blood pressure and tail blood flow of both spontaneously hypertensive rats and control Wistar Kyoto rats were measured by the tail-cuff metho...
Article
Full-text available
S-1-Propenyl-L-cysteine (S1PC) is a stereoisomer of S-Allyl-L-cysteine (SAC), an important sulfur-containing amino acid that plays a role for the beneficial pharmacological effects of aged garlic extract (AGE). The existence of S1PC in garlic preparations has been known since the 1960's. However, there was no report regarding the biological and/or...
Article
Aged garlic extract (AGE) has been shown to improve hypertension in both clinical trials and experimental animal models. However, the active ingredient of AGE remains unknown. In the present study, we investigated the antihypertensive effects of AGE and its major constituents including S-1-propenylcysteine (S1PC) and S-allylcysteine (SAC) using spo...
Article
Scope: In this study, we investigated the effect of aged garlic extract (AGE) on the high level of blood glucose in Tsumura Suzuki Obese-Diabetes (TSOD) mice. Methods and results: TSOD mice were fed standard diet with or without 2% AGE for 19 weeks. AGE treatment lowered the blood glucose level and significantly reduced the plasma level of glyca...
Article
The present study investigated whether a water-soluble extract from the culture medium of Ganoderma lucidum mycelia (Japanese: Reishi or Mannentake) (designated as MAK) exerted a protective effect against induction of aberrant crypt foci (ACF) by azoxymethane (AOM) and small-intestinal damage induced by the anticancer drug 5-FU. Six-week-old male F...
Article
A dietary supplement containing garlic fermented with Monascus pilosus (MGFE) may be useful to decrease the lipid concentrations in serum without serious adverse effects. The aim of the study was to assess whether MGFE decreases serum lipid contents in volunteers with mild hyperlipidemia in a randomized, double-blind, placebo-controlled, parallel-g...
Article
Lancemaside A, a triterpenoid saponin isolated from the roots of Codonopsis lanceolata, has been reported to ameliorate the reduction of blood testosterone levels induced by immobilization stress in mice. In the present study, we investigated the metabolism and absorption of lancemaside A in mice. After oral administration of lancemaside A at 100 m...
Article
The present study investigated whether a water-soluble extract from the culture medium of Ganoderma lucidum (Reishi) mycelia (MAK) is able to protect the small intestine against damage induced by anti-cancer drugs. Six-week-old male B6C3F1/Crlj mice were fed a basal diet (MF) alone or with various doses of MAK or Agarics blazei Murrill (AGA) beginn...
Article
We developed a rapid and simple analytical method for the simultaneous determination of seven 3,28-bidesmosidic triterpenoid saponins in the roots of Codonopsis lanceolata. The saponins are lancemaside A, lancemaside B, lancemaside C, lancemaside E, lancemaside G, foetidissimoside A, and aster saponin Hb. Root samples were extracted with 50% methan...
Article
Liquid chromatography coupled with sequential mass spectrometry (LC–MSn ) has been used to identify 3,28-bidesmosidic triterpenoid saponins, lancemaside A (1), foetidissimoside A (2), aster saponin Hb (3), lancemaside E (4), lancemaside B (5), lancemaside F (6), lancemaside G (7), lancemaside C (8), and lancemaside D (9) in the roots of Codonopsis...
Article
Lancemaside A is a saponin that inhibits decreases in blood testosterone level and thus prevents or ameliorates symptoms associated with male climacteric disorder. Our initial attempt to preparative isolation of lancemaside A from the saponin fraction of Codonopsis lanceolata roots by a preparative HPLC did not give a clear result. To develop a sim...
Article
In the course of the development of new designer foods using the roots of Codonopsis lanceolata, we found that hot-water extracts of C. lanceolata recovered decreased testosterone levels in the blood and accelerated the restoration of reproductive dysfunction induced by hyperthermic treatment in male mice. Thus we studied the constituents of the po...
Article
More than three thousand publications in the past have confirmed the efficacy of garlic for the prevention and treatment of a variety of diseases, acknowledging and validating its traditional uses. Garlic is also used for the treatment of fatigue, although the mechanism involved remain unclear. The anti-fatigue function of garlic may be closely rel...
Article
Cycloalliin, an organosulfur compound found in garlic and onion, has been reported to exert several biological activities and also to remain stable during storage and processing. In this study, we investigated the pharmacokinetics of cycloalliin in rats after intravenous or oral administration. Cycloalliin and its metabolite, (3R,5S)-5-methyl-1,4-t...
Article
Aged garlic extract (AGE) has recently received attention as a potent anti-fatigue agent. The principal aim of this study was to elucidate the mechanism responsible for the ameliorating effect of AGE on physical fatigue in rats caused by repeated endurance exercise on a mechanical treadmill apparatus. Rats were subjected to endurance exercise 5 tim...
Article
We newly produced an aged garlic extract preparation combined with ginseng, oriental bezoar, antler velvet, cuscuta seed and epimedium herb (LEOPINROYAL; LER), and examined whether it would improve peripheral blood circulation, as determined by the cooling-rewarming test in mice, or by a four-week trial in humans. In the mouse cooling-rewarming tes...
Article
Monascus Garlic Fermented Extract (MGFE) is a unique material produced from garlic fermented using Monascus pilosus. The present study was designed to examine the effects of MGFE on the development of hyperlipidemia and atherosclerotic lesions. Rabbits in a control group and a MGFE group were fed an experimental diet containing 0.25% cholesterol wi...
Article
We investigated the pharmacologic activities of four garlic preparations, raw garlic juice (RGJ), heated garlic juice (HGJ), dehydrated garlic powder (DGP) and aged garlic extract (AGE). The study used three animal models, i.e., testicular hypogonadism (hypospermatogensis and impotence) induced by warm water treatment, intoxication of acetaldehyde...
Article
Maternal sound stress (800 Hz; 77 dB, every other minute for 15 min/day, from day 10 to 18 of gestation), combined with forced swimming stress (15 min/day), was found to cause potentiation of sound-induced loss of locomotor activity, referred to as emotional behavior, of male offspring, but not that of female offspring, at 4 weeks of age. Maternal...
Article
Maternal sound stress (800 Hz; 77 dB, every other minute for 15 min/day, from day 10 to 18 of gestation), combined with forced swimming stress (15 min/day), was found to cause potentiation of sound‐induced loss of locomotor activity, referred to as emotional behavior, of male offspring, but not that of female offspring, at 4 weeks of age. Maternal...
Article
We determined the effect of Aged Garlic Extract (AGE) on damage caused to immune function by a psychological stress using a communication box. After four days of a psychological stress, a decrease in spleen weight and spleen cells was observed in the psychological stress-exposed mice as compared normal mice (non-stress). AGE significantly prevented...
Article
The effect of aged garlic extract (AGE) on stress induced hyperglycemia was investigated using the immobilization stress model in mice. After the exposure to immobilization stress for 16 hr per day for 2 consecutive days, the adrenal glands of the mice hypertrophied, and their serum glucose level and corticosterone secretion became elevated, but in...
Article
We found that Aged Garlic Extract (AGE) could be a significant immuno-potentiator, and could exhibit anti-tumor activities through immune modulation. Consequently, AGE stimulated the proliferation of mouse spleen cells and the release of cytokines, such as IL-2, TNF-alpha and IFN-gamma, increased NK activities, and enhanced phagocytosis of peritone...
Article
To examine the effect of Aged Garlic Extract (AGE) on the function of mast cells and activated T lymphocytes, we adopted the in vitro histamine release system, the in vivo IgE mediated skin reaction system and the in vivo late phase reaction system. Consequently, at 1.25, 2.5, and 5.0% (v/v), AGE dose-dependantly inhibited the antigen specific hist...
Article
The effect of several kinds of garlic preparations such as raw garlic juice (RGJ), heated garlic juice (HGJ), processed garlic powder (PGP) and aged garlic extract (AGE) on both physiological and psychological stress were investigated using four stress models in mice: forced swimming test, mechanical treadmill running, immobilization stress test, a...
Article
The pharmacokinetic behavior of S-allylcysteine (SAC), one of the biologically active transformation products from garlic, was investigated after oral administration to rats, mice, and dogs. SAC was rapidly and easily absorbed in the gastrointestinal tract and distributed mainly in plasma, liver, and kidney. The bioavailability was 98.2, 103.0, and...

Citations

... Studies in rodents have shown that during pregnancy, maternal stress due to restraint, noise, light, and heat may be associated with adverse effects in the mother and be detrimental to embryonic/fetal development and postnatal (Golub and Domingo 1996;Miller et al. 2002;Nishio et al. 2001). In addition, it is also well known that exposure to certain metals during pregnancy can also cause developmental toxicities (Domingo 2001) and adverse birth outcomes in human, such as preterm birth, low birth weight, and small gestational age . ...
... AGE contains several water-soluble sulfur compounds such as S-1-propenylcysteine (S1PC) 42,43 . Recently, S1PC has been shown to inhibit IL-6 production through autophagy activation, improve peripheral blood circulation by increasing the NOx production, and maintain endothelial barrier function [44][45][46] . We have previously reported that AGE inhibits lipid deposition in apolipoprotein E knockout (ApoE-KO) mice, an atherosclerosis model 47,48 . ...
... Previous studies have demonstrated the beneficial effect of AGE on hypertension (8)(9)(10)(11)(12), atherosclerosis (13)(14)(15), metabolic syndrome (16), and gingivitis (17). Furthermore, recent studies have revealed that AGE and its constituents, SAC and S1PC, exert such desirable effects as anti-hypertension (18)(19)(20)(21)(22), cardioprotection (23), antioxidation (24)(25)(26)(27), antiaging (27,28), i m munomodulation (29)(30)(31), a nti-fatig ue (32,33), anti-stress (34,35), anti-inflammation (36)(37)(38)(39), and liver protection (40). ...
... Hypertension is often accompanied by progressive kidney damage [22], histidine can attenuate salt-sensitive hypertension and kidney damage by improving metabolic patterns [23], and higher intakes of histidine were signi cantly associated with improvement of peripheral and central blood pressure [24]. A recent study showed that altered histidine metabolism exerts the antihypertensive effect via the central histamine H3 receptor [25]. The above results indicated that a high level of histidine may contribute to the excellent tolerability to higher blood pressure in drug users. ...
... Di Donfrancesco [40] sarımsak ve karabiberin yüksek aroma özelliklerinin olduğunu belirlemiştir. Yamato ve ark [41] da uygun dozlarda kullanıldığında sarımsak ekstraktının lezzet artıcı etkisinin olduğunu ve köpek sağlığına faydalı olduğunu belirlemişlerdir. Yumesa ve kontrol maması arasındaki tercih farklılığı önemsiz düzeydedir. ...
... It was found that mice lacking MyD88 were less sensitivity to LPS (46). S-1-propenylcysteine (S1PC) inhibited IL-6 production by inducing the degradation of MyD88 (47). In fish, we reported that IRF3 negatively regulates NF-κB signaling by targeting MyD88 (40), and micro-RNAs directly target MyD88 and affect the NF-κB signaling through posttranscriptional regulation (35,36). ...
... S-1-propylenecysteine was proven to increase peripheral blood circulation and lower systolic blood pressure in spontaneous hypertension rats while not affecting control rats' systolic blood pressure. [166] In another investigation, Bacillus subtilis transformed nitrites in fermented garlic extract (FGE) into NO in vivo. Furthermore, NO decreased systolic blood pressure in spontaneously hypertensive rats through the soluble guanylyl cyclase (sGC)-cyclic guanosine monophosphate (cGMP)-protein kinase G (PKG) signaling pathway. ...
... The OSCs, responsible for the pungent odor and spicy flavor, are among the major contributors to its pharmacological properties [11]. The existence and potency of the bioactive constituents of garlic vary based on its method of preparation and extraction [12]. The main sulfur-containing compounds in garlic are S-allyl-L-cysteine sulfoxide (Alliin) and γ-glutamyl-S-allyl-L-cysteine (GSAC). ...
... In this context, SHRs have been used as a model of hypertension to examine the antihypertensive effects of several herbal extracts such as Tengfu Jiangya (a combination of Uncaria rhynchophylla and raphani semen), Alismatis Rhizome (Oriental water plantain rhizome), and aged garlic extracts, in order to discover therapeutic targets of hypertension by applying metabolomics and proteomics techniques [17][18][19][20]. Such omics approaches represent a powerful and high throughput technique already applied in medical diagnostics and basic research [21,22]. ...
... Downregulated AMPK activity may affect insulin sensitivity, which could indirectly contribute to the pathogenesis of T2DM (Towler and Hardie 2007;Friedrichsen et al. 2013). According to research by Miki et al., feeding 2% aged garlic extract for 19 weeks significantly reduced plasma levels of glycated albumin and enhanced IR, which was partly attributed to an increase in the phosphorylation of AMPK in muscle (Miki et al. 2017). Moreover, the AMPK signaling pathway interacts with many molecules including GLUT4 (Giacoman-Martínez et al. 2021). ...