Min-Long Cui's research while affiliated with Zhejiang A&F University and other places
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Publications (8)
The unique capitulum of Asteraceae has important ornamental and research value. Few studies have described the complex molecular mechanism of flower development. In this study, SvGLOBOSA(SvGLO), the MADS-box gene of Senecio vulgaris, was identified by screening the transcriptome data, and its function was examined. The gene structure was analyzed a...
A Plantaginaceae flowering plant, Chelone glabra, is different from Arabidopsis thaliana and cotton (Gossypium hirsutum), as it produces fibers on the anther surface. However, the evolutionary molecular mechanism of how fiber development is controlled in the stamen is unclear. MYB genes are essential transcription factors for trichome and fiber dev...
Anthocyanins are the largest group of water-soluble pigments and beneficial for human health. Although most plants roots have the potential to express natural biosynthesis pathways required to produce specialized metabolites such as anthocyanins, the anthocyanin synthesis is specifically silenced in roots. To explore the molecular mechanism of abse...
Genetic transformation is a powerful tool to study gene function, secondary metabolism pathways, and molecular breeding in crops. Cotton ( Gossypium hirsutum L.) is one of the most important economic crops in the world. Current cotton transformation methods take at least seven to culture and are labor-intensive and limited to some cultivars. In thi...
Transformation approach is a useful tool for the study of gene function, the mechanism of molecular regulation, and increase usefulness of components by reverse genetic approach in plants. In this study, we developed a stable and rapid method for Agrobacterium-mediated transformation of a medicinal plant Chelone glabra L. using leaf explants. Stabl...
In this study, we developed a rapid and efficient method for in vitro propagation and Agrobacterium tumefaciens-mediated transformation of Digitalis purpurea L. (syn. foxglove), an important medicinal plant. Mature leaf explants of D. purpurea were used for 100 % adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 1...
The red leaf coloration of Empire Red Leaf Cotton (ERLC) (Gossypium hirsutum L.), resulted from anthocyanin accumulation in light, is a well known dominant agricultural trait. However, the underpin molecular mechanism remains elusive. To explore this, we compared the molecular biological basis of anthocyanin accumulation in both ERLC and the green...
We investigated the transcriptional regulation of anthocyanin biosynthesis in hairy roots system by ectopically expressing Rosea1 and Delila and we found something different from previous research. The RT-PCR results revealed that Rosea1 could activate early and late biosynthetic genes tested, including CHS, DFR and ANS. Delila enhanced the express...
Citations
... Only by combining MYB TFs with MYB motif of target gene promoter can the target gene be expressed and play a role [42,43]. By using electrophoretic mobility shift assay (EMSA) to verify the binding of FfMYB15 to the FfCEL6B, it was found that only one probe band was displayed, when a biotin-labeled probe was added. ...
... P. volubilis is a woody vine oil crop whose seeds contain approximately 45%-50% lipids and have high economic value [19][20][21][22][23][24]. In recent years, using Agrobacterium rhizogenes to induce transgenic hairy roots has been successful in a variety of woody plants [25][26][27][28]. Those studies provide new insight into the genetic transformation of recalcitrant woody plants such as P. volubilis. ...
... A. tumefaciens strains GV3101/35S::CgMYB4 and GV3101/ Cgmyb4 were grown in 3 mL of LB medium containing 100 mg/L kanamycin at 28 °C for 24 h, diluted 1:40 in liquid MS medium and then transformed. Transformation of C. glabra was performed using young leaf explants as material according to the method described by Gao et al. (2015). After 4~6 weeks of culture on selection medium, the emerged shoots were transferred to rooting medium (MS medium supplemented with 50 mg/L kanamycin and 200 mg/L cefotaxime). ...
... Shoot regeneration and transformation of β-glucuronidase gene (GUS) and kanamycin selection marker gene nptII 50-60 [66] 6 ...
... The genus Gossypium belongs to the Malvaceae family and contains more than 50 diploid and allotetraploid species. These species are quite diverse in terms of the color of leaf, flower, and fiber (Gao et al., 2013;Long et al., 2019;Shao et al., 2021;Sun et al., 2021). Genetic and functional investigations have uncovered several genes involved in the biosynthesis and accumulation of anthocyanins in different cotton tissues. ...
