Milkessa HaileMariam's research while affiliated with Addis Ababa University and other places

Publications (6)

Article
Full-text available
Differential diagnosis of tuberculosis (TB) and latent TB infection (LTBI) remains a public health priority in high TB burden countries. Pulmonary TB is diagnosed by sputum smear microscopy, chest X-rays, and PCR tests for distinct Mycobacterium tuberculosis (Mtb) genes. Clinical tests to diagnose LTBI rely on immune cell stimulation in blood plasm...
Preprint
Differential diagnosis of tuberculosis (TB) and latent TB infection (LTBI) remains a public health priority in high TB burden countries. Pulmonary TB is diagnosed by sputum smear microscopy, chest X-rays, and PCR tests for distinct Mycobacterium tuberculosis (Mtb) genes. Clinical tests to diagnose LTBI rely on immune cell stimulation in blood plasm...
Article
Full-text available
Background: Tuberculosis (TB) is one of the major public health problems in Ethiopia. Data on genetic diversity and resistance profile of circulating TB strains is critical for informing the national TB control program. Methods: A cross-sectional study was conducted on 213 smear positive pulmonary TB patients between 2015 and 2016. Sputum sample...
Article
The success of shotgun proteomic analysis depends largely on how samples are prepared. Current approaches such as gel-, solution- or filter-based, although being extensively employed in the field, are time-consuming and less effective with respect to the repetitive sample processing, recovery, and overall yield. As an alternative, the suspension tr...
Article
Full-text available
Accurate diagnosis and early treatment of tuberculosis (TB) and latent TB infection (LTBI) are vital to prevent and control TB. The lack of specific biomarkers hinders these efforts. This study's purpose was to screen immunological markers that discriminate M. tuberculosis (Mtb ) infection outcomes in an endemic setting of Ethiopia. Whole blood fro...

Citations

... These unique 23 signatures can accurately identify materials. Raman spectroscopy has been widely applied for explosive 24 identification and, most notably, was the key method used to identify water on Mars [12]. In the last 25 decade, Raman spectroscopy in various configurations has been thoroughly investigated to detect 26 multiple diseases, including cancer, endoscopy, and skin disorders [13][14][15]. ...
... The majority of isolates (95%; 97/102) were M. tuberculosis , which was in line with results from previous studies ( Addo et al., 2017 ;Damena et al., 2019 ). Moreover, our study identified 3.9% (4/102) of isolates as M. africanum , consistent with the 2-20% previously reported in Ghana ( Acquah et al., 2021 ;Addo et al., 2017 ;Asante-Poku et al, 2016 ). ...
... Preparation of proteomic samples: CHO and MMP-enriched host cells were lysed and trypsin digested using S-TRAP as previously described. 31,32 Peptides were labeled with 6-plex TMT (Thermo Fisher Scientific) according to manufacturer's instructions ( Figure S7 and Supplementary Methods). ...
... Using cytokine antibody arrays, we surveyed the blood plasma of TB, LTBI, and negative community controls (NCC), from the same cohort under study here, and identified RANTES as a potential plasma biomarker of LTBI. This chemokine was differentially abundant with statistical significance (LTBI vs TB), with and without prior TB antigen stimulation (Teklu et al., 2018a). ...