Milica Radisic’s research while affiliated with University of Toronto and other places

... Recent studies have also demonstrated the potential of macrophages to address the dearth of vascularization observed in cardiac organoids. Landau et al. incorporated hiPSC-derived macrophages into heart-on-achip, observing the formation of stable and perfusable microvasculature within cardiac tissue [118]. RNA-seq analysis revealed an upregulation of pro-angiogenic and cardiac maturation markers. ...

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Engineering Cardiology with Miniature Hearts

... In recent years, biodegradable scaffolds have been widely used in tissue engineering (TE) applications [1][2][3][4]. An ideal TE scaffold is defined as a porous structure, which is biocompatible, bioactive and possesses adequate mechanical and physical properties, convergent to the tissues in place of implantation [5][6][7]. Mimicking the complex architecture and mechanical properties of the native tissue is one of the most important paradigms in tissue engineering. 3D printing can be implemented to fulfill this criterion. ...

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Nanohydroxyapatite adhesion to low temperature plasma modified surface of 3D-printed bone tissue engineering scaffolds - qualitative and quantitative study

... These microfluidic devices provide an environment that mimics the mechanical, chemical and physical microenvironments of tissues, surpassing the limitations of static 2D cultures and reducing reliance on animal models 5-7,9 . Many microfluidic devices rely on materials like polydimethylsiloxane (PDMS) 7 , due to its optical clarity, biocompatibility and ease of fabrication [10][11][12][13] . PDMS offers unique properties compared to thermoplastics, such as elasticity and gas permeability. ...

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Sorption and release of small molecules in PDMS and COC for Organs on chip

... Polycaprolactone (PCL) is a type of polymer that is highly biodegradable, can be 3D-printed, accepts surface modification well, and shows adaptability and operability [18,19]. However, cells show poor adhesion, proliferation, and differentiation on PCL due to its hydrophobicity and low wettability [20,21]. Research has been conducted to improve the shortcomings of PCL scaffolds by adding various bioactive substances, such as hydroxyapatite and betatricalcium phosphate [22,23]. ...

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Effect of 3D-printed polycaprolactone/osteolectin scaffolds on the odontogenic differentiation of human dental pulp cells

... Today biotechnology is emphasizing the use of genetically modified organisms for bioremediation. 8 The treatment of these pesticides should be done this way that the degradation of the compound will not generate intermediates. The existing technologies are using physical or chemical ways to treat the pesticides, and according to FAO, they could cost up to 3000-4000 USD/tons. ...

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Analysis of Microbial Degradation of Pesticides

... The scaffolds are specifically designed to provide essential structural support while promoting tissue regeneration. By mimicking the natural extracellular matrix, they create an environment that encourages cell growth and repair, ultimately assisting the tissue in recovering its functional capabilities [6][7][8]. ...

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In Vitro Characterization of 3D-Printed PLA/CPO Oxygen Releasing Scaffolds: Mechanical and Biological Properties for Bone Tissue Engineering

... On the other hand, research on myocardial tissue engineering using cell sheets [8,9] and cell spheroids [10,11] without extracellular matrix has been conducted. Although remarkable progress has been made in myocardial tissue engineering, rapid construction and transplantation efficiency have not yet been demonstrated [12]. For the purpose of cell therapy, we developed a technique involving lowering the temperature to induce hydrophilicity on the culture dish surface, which allows the cells to be peeled off and collected as a sheet. ...

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Cardiomyocyte sheet stacking using fibrin enables high-speed construction of three-dimensional myocardial tissue and high transplantation efficiency

... [5b,d] More recently, we have also demonstrated the use of elastomer-bonded standing AuNWs for strain and electrochemical biosensors. [14] On the other hand, fibers are crucial materials with numerous technical applications ranging from textile products, membrane filtration, [15] catalysis, [16] tissue engineering, [17] and flexible electronics. [18] Previously, methods for preparing mesoporous silica fibers include electrospinning, [19] template method [20] and spontaneous growth. ...

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Cat‐Tail‐Like Mesostructured Silica Fibers Decorated with Gold Nanowires: Synthesis, Characterization, and Application as Stretchable Sensors

... When reviewing methods to introduce new CMs to the heart, two main delivery routes have been accomplished: transplantation via intramyocardial (or intra-scar) injection (as single-cell solution or small aggregates) and the epicardial application of preformed 3Dconstructs ( Figure 3). Both approaches carry their advantages and drawbacks (Feric and Radisic, 2016;Kadota and Shiba, 2019). Although efficacy was repeatedly proven in small animal models for both delivery approaches, the CM injection strategy has been mainly tested in NHP models (Tables 1, 2). ...

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Non-human primate studies for cardiomyocyte transplantation—ready for translation?

... Furthermore, treatment of constructs with a combination of electrical pacing and insulin-like growth factor 1 (IGF-1) supplementation has been shown to further improve the contractile properties and connexin-43 expression in engineered heart constructs over and above levels observed in response to either factor applied independently (Park et al. , 2014). The integration of three-dimensional cardiac biowires (derived from pluripotent stem cell sources) with long-term stimulation protocols for up to 4 weeks in vitro promotes improvements in sarcomeric banding (Figure 3E,F), excitation thresholds, and conduction velocities (Nunes et al. , 2013, Xiao et al. , 2014). Cells from these preparations also exhibit larger hERG currents, more negative resting membrane potentials, and larger capacitances (suggesting larger cells) than unstimulated controls, further indicating the improved functionality of cardiomyocytes exposed to long-term electrical stimulation (Nunes, Miklas, 2013). ...

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Human iPSC-derived cardiomyocytes and tissue engineering strategies for disease modeling and drug screening