Milena Malisic's research while affiliated with Heinrich-Heine-Universität Düsseldorf and other places

Publications (3)

Article
Full-text available
The primary visual cortex (area V1) is an extensively studied part of the cerebral cortex with well-characterized connectivity, cellular and molecular architecture and functions (for recent reviews see Amunts and Zilles, Neuron 88:1086–1107, 2015; Casagrande and Xu, Parallel visual pathways: a comparative perspective. The visual neurosciences, MIT...
Article
Full-text available
Regulated intracellular proteostasis, controlled in part by proteolysis, is essential in maintaining the integrity of podocytes and the glomerular filtration barrier of the kidney. We applied a novel proteomics technology that enables proteome-wide identification, mapping, and quantification of protein N-termini to comprehensively characterize clea...

Citations

... MALDI MSI is a powerful label-free technique for mapping the spatial distribution of proteins in fresh frozen (FF) cryosections, as well as in formalin-fixed paraffin-embedded (FFPE) tissues, based on their tryptic fragments after on-tissue trypsinization. MSI, often based on MALDI tandem mass spectrometry, was successfully applied for proteomic analysis of various tissues (Table 2), such as post-mortem human brain samples (Figure 4a,d) [75,76], human articular cartilage [77] (Figure 4f), rats intestine [78] (Figure 4b), human atherosclerotic carotid [79], rat brain [29,80], normal and melanoma pig skin [81] (Figure 4g), mouse pituitary gland (Figure 4e) [82], or mouse model glioblastoma [83] ( Figure 4c). ...
... 15 Proteolytic damage of podocytes is a common effector pathway for glomerular diseases. [16][17][18] Recent studies in experimental models, suggest that the maintenance of renal filtration barrier largely relies on proteases as key regulators of podocyte integrity and kidney function. 19 Evidence that dysregulation of proteolytic pathways is a key driver for human disease signifying proteases as promising targets for tailored therapeutics. ...