Mikael Bauer's research while affiliated with Lund University and other places

... This in turn suggested that the integrity of the ribonucleoprotein particles is affected by calcium. This finding, together with the fact that intracellular calcium oscillations regulate critical steps in protein synthesis [13,14,28], prompted us to examine the relationship between calcium and RNP integrity in more detail. Rat brain RNPs are a population of heterogeneous size and density, so we first tested for gross changes in RNP structure by assessing sedimentation profiles in sucrose gradients in the presence and absence of calcium (Fig. 2C). ...

... Dysfunctions in Ca 2+ /CaM based regulatory mechanisms are linked to several human diseases [49][50][51][52]. The key components of the CRAC machinery, STIM and Orai channel proteins have been shown to interact with Ca 2+ /CaM [16,46,[53][54][55][56][57][58][59][60]. However, Ca 2+ /CaM, STIM1 and Orai1 interaction sites and the underlying regulatory mechanism of SCDI still remain unclear. ...

... Secretagogin is a Ca 2+ sensor protein ([Ca 2+ ] 0.5 is of ~25 µM for secretagogin in physiological salt buffers), which by definition induces protein-protein interactions through conformational change upon Ca 2+ binding followed by downstream signaling to control discrete cellular functions (Schwaller 2010). Secretagogin's in vitro interactome initially included synaptosomal-associated protein 25 kDa (SNAP-25) (Rogstam et al. 2007) with the more recent discovery of vesicle cargo, traffic and docking/release proteins (Bauer et al. 2011a,b, Romanov et al. 2015. Particularly, the 5th EF-hand domain can interact with cytoskeletal components (microtubules) (Maj et al. 2010, Yang et al. 2016. ...

... This information is useful in understanding the changes occurring on the surface of the NWs in each specific matrix, which will dictate and predict their role in a biological environment. 101 ...

... The first classical example of this property of fragment complementation is ribonuclease which was reconstituted 1958 from two separate polypeptide fragments with retained fold and function 13 . This exercise has been followed by many reports of proteins that are stable enough that their structure and function can be reconstituted through the non-covalent assembly of fragments comprising two or more subdomains [14][15][16][17][18] . The individual subdomains may be more or less unfolded in isolation but their folding is energetically coupled to association with the other subdomain. ...

... Leptin is an important hormone produced by white adipose tissue to regulate energy expenditure in human body (Jönsson et al. 2015). Leptin resistance has been hypothesized as one of the major obesity risks, and it has been associated with gluten diet. ...