Mihai Burlibasa’s research while affiliated with Carol Davila University of Medicine and Pharmacy and other places

Digital technologies represent new alternatives in prosthetic rehabilitation of all kind of maxillary or mandibular spans. This article is composed of two parts, in which different technological aspects will be presented regarding the prosthetic restoration of frontal maxillary areas using the CAD-CAM technology, but using different material for resistance framework, both veneered with ceramic materials to restore the aesthetics. The main material that will be used are: Co-Cr dental alloy and zirconium dioxide (zirconia).

Objective: This study aims to evaluate cell proliferation capacity and micronuclei incidence in the presence of nickel–chromium (Ni-Cr)-based dental alloys, with and without the addition of beryllium (Be). The use of these alloys in dental prosthetics is widespread; however, the potential risks associated with their genotoxicity and cytotoxicity require further investigation. The study seeks to provide insight into the safety of these materials and their long-term impact on the health of both patients and dental professionals. Methods: The study was conducted through a comparative analysis of genotoxicity and cytotoxicity using human lymphocyte cultures exposed to two types of Ni-Cr-based dental alloys, one containing beryllium and the other without beryllium. The evaluations were performed according to the OECD Test No. 487 guideline, employing the micronucleus assay and cell proliferation assay. Lymphocytes were exposed to three different alloy concentrations (5 mg/mL, 10 mg/mL, and 20 mg/mL), and the effects on genetic material were analyzed microscopically. Descriptive statistics (mean, standard deviation, and variance) were calculated, and one-way ANOVA was used to assess statistical significance between groups, with a significance threshold of p < 0.05. Results: A significant increase in cytotoxicity and micronuclei incidence was observed in the samples containing beryllium compared to those without beryllium. Statistical analysis revealed significant differences (p < 0.001) between the test and control groups and between different concentrations. Additionally, a direct proportional relationship was noted between alloy concentration and the intensity of genotoxic effects. Microscopic analysis confirmed genetic material damage, indicating a potentially increased risk associated with the use of this type of dental material. Conclusions: The data obtained suggest that Ni-Cr-based dental alloys containing beryllium may present a significant risk of genotoxicity and cytotoxicity. Therefore, the selection of materials used in dental prosthetics should be based on solid scientific evidence, and the use of these alloys should be approached with caution. The study highlights the need for further research to better understand the long-term impact of these materials on human health.

The aim of this study was to compare the interproximal contact tightness of lateral teeth after restoring adjacent proximal walls with four types of direct adherent biomaterials. Distal and mesial boxes were prepared on 160 artificial right first and second upper molars. Each set of 40 pairs of boxes was restored using one bulk biomaterial: Equia Forte Fil HT (GC), Cention® Forte (IVOCLAR VIVADENT), Admira Fusion x-tra (VOCO), or 3MTMFiltekTM One Bulk Fill. The mean difference in the passing-through force varied from sound to restored surfaces immediately after application, as well as at 7 and 14 days after: Equia Forte Fil HT—4.07 ± 0.01, 4.08 ± 0.01, and 4.11 ± 0.01; Cention® Forte—3.30 ± 0.01, 3.50 ± 0.01, and 3.56 ± 0.01; Admira Fusion x-tra—4.10 ± 0.01, 4.13 ± 0.01, and 4.13 ± 0.01; 3MTMFiltekTM One Bulk Fill—4.08 ± 0.01, 4.09 ± 0.01, and 4.07 ± 0.01 (p < 0.05). The passing-through force of the restored contact areas showed significantly higher values when compared to those for the sound surfaces, and among them, all biomaterials presented similar values, except for Cention® Forte. The potential clinical relevance of this study relates to better knowing the most appropriate restorative material for large proximal caries on adjacent surfaces from the outset of the treatment protocol.

Tooth autotransplantation is a procedure involving the surgical repositioning of a tooth or dental germ from one site in the mouth to another within the same individual. A successful procedure requires a donor tooth with healthy cementum and periodontium, gentle surgical maneuvers, a well-fitted neoalveolus, and a short extraalveolar time of the donor tooth. Digital technology increases the accuracy and predictability of the tooth autotransplantation procedure. Surgical templates generate a more precise neoalveolus, with good stability of the donor tooth, and decrease the surgical time. Using a donor tooth replica ensures an optimal morphology of the new alveolus, thus reducing the injury of the root and the extraoral time of the donor tooth. This case report presents the tooth autotransplantation technique, that was employed to reposition a maxillary premolar with two fused roots on a mandibular recipient site. Digital planning, two surgical guides (one for each root), and a 3D printed tooth replica were used to ensure good accuracy, prognosis, and reduced treatment time. The procedure is conservative and offers considerable advantages to the patient, such as retaining natural teeth and proprioception.

Farmaciști și chimiști francezi în perioada napoleoniană (III)

Practical aspects in restoring the morphology and functionality of the dento-maxillary system using full-coverage prosthetic restorations (Part I) Asistente medicale – eroine ale Primului Război Mondial (Partea I)

Background: Blood borne diseases are important diseases that can transmit their etiological agents in the medical office. One of the most feared due to its contagious remains hepatitis B virus (HBV). This infection is vaccine preventable. The present study aimed to assess the HBV infection and HBV vaccination status of Romanian dental professionals exposed to Toxoplasma gondii, a very common parasitic worldwide infection. We assessed HBV vaccination status, attitudes, knowledge, and fears. Methods: In 2024, an analytical cross-sectional observational survey was carried out in six months, from February to August, in dental offices in Romania. Using SPSS (version 23), data collected through a selfadministered questionnaire were examined. Where applicable, chi-square tests were employed. Results: Responses from 1708 individuals were examined. The median age of the cohort under study was 31.22 ± 12.7 years (range: 18–76 years). 742 (43.44%) of the participants had worked in dental offices for more than three years, and 1470 (86.07%) were from urban dental offices. 322 (18.85%) nurses, 308 (18.03%) dental technicians, and 392 (22.95%) dentists were present. 1120 dentists (65.57%) had received an HBV vaccination. 420 (24.59%) had received an HBV vaccination before to beginning work at the dental clinic. The dental technician category had the highest rate of HBV not-vaccination, at 154 (13.75%). The urban dental office had a significant (p<0.05) correlation with HBV vaccination status (OR = 0.38; 95% CI: 0.28-0.51). More than half of the respondents, 1456 (85.25%), had not been exposed to HBV while working in a dental office, and 882 (51.64%) were unaware of post-exposure HBV prophylaxis. 98 dentists (5.74%) were unaware of the HBV transmission mechanism. Of the dental professionals who worked with or for patients infected with HBV, 812 (47.54%) expressed fear of contracting the virus. Testing for antiHBs antibodies (OR = 0.38; 95% CI:0.29–0.51), knowledge of pre-exposure to HBV prophylaxis (OR = 0.49; 95% CI:0.37-0.67), and the respondents' role/occupation in the dental office (OR = 0.70; 95% CI:0.56–0.88) were all significantly correlated with the fear of contracting HBV (p<0.05, nonparametric Chi-square test). Conclusions: The current study found disparities in knowledge, attitudes, and opinions within the group under investigation, as well as a low level of HBV immunization in the dentist’s office. The dental technicians were terrified of the possibility of contracting HBV and had received fewer HBV vaccinations. They were unaware of the possibility of contracting HBV. Dental professionals in Romania should be encouraged to learn about HBV prevention before and after exposure. The level or titer of protective antiHBs antibodies should be measured to lessen the concern of contracting HBV while working with or for patients who have the virus. Romanian dental offices need to enhance their HBV vaccination status.

A mixed prosthetic restoration combines the strength provided by the metal component with the aesthetic appearance very similar to that of natural teeth provided by the physiognomic component. The metal component is made of dental alloys (noble, stainless Ni-Cr, Co-Cr, etc.), and the physiognomic component can be made of materials such as ceramic masses, composite diacrylic resins, etc. This material presents a comparative study of the manufacturing technology in the case of metal-composite versus metal-ceramic prosthetic restorations.

Digital technologies represent new alternatives in prosthetic rehabilitation of all kinds of maxillary or mandibular spans. This article is composed of two parts, in which different technological aspects will be presented regarding the prosthetic restoration of frontal maxillary areas using the CAD-CAM technology, but using different material for resistance framework, both veneered with ceramic materials to restore the aesthetics. The main materials that will be used are: Co-Cr dental alloy and zirconium dioxide (zirconia).