Middelanis J's research while affiliated with Ruhr-Universität Bochum and other places

Publications (41)

Article
Objective: To test a possible neuroprotective activity of 17β-estradiol in the neonatal rat brain exposed to hypoxic-ischemia (controlled hypoxia after unilateral carotid artery ligation). Methods: Seven-day-old Wistar rats underwent ligation of the left common carotid artery followed by 80 minutes hypoxia in 8% oxygen inducing an ipsilateral br...
Article
Full-text available
Brain damage around birth may cause lifelong neurodevelopmental deficits. We examined the therapeutic potential of human umbilical cord blood-derived mononuclear cells containing multipotent stem cells to facilitate motor recovery after cerebral hypoxic-ischemic damage in neonatal rats. Left carotid artery ligation followed by 8% O(2) inhalation fo...
Conference Paper
Full-text available
Hypoxic-ischemic cerebral damage is an important contributor to perinatal mortality and morbidity. Experimental evidence has shown that neuroprotective strategies using pharmacologic agents can alleviate perinatal brain damage. However, so far no convincing strategies are available for the regeneration of damaged structures of the perinatal nervous...
Article
Full-text available
We tested the neuroprotective effects of creatine against hypoxic-ischemic injury in the immature brain. Hippocampal slices were prepared from fetal guinea pigs at 0.9 gestation and incubated in artificial cerebrospinal fluid (aCSF) equilibrated with carbogen. Slices were subjected to oxygen-glucose deprivation (OGD) for 30 or 40 minutes. Two hours...
Article
Full-text available
Children undergoing perinatal brain injury often suffer from the dramatic consequences of this misfortune for the rest of their lives. Despite the severe clinical and socio-economic significance, no effective clinical strategies have yet been developed to counteract this condition. This review describes the pathophysiological mechanisms that are im...
Article
Perinatal brain damage is associated not only with hypoxic-ischemic insults but also with intrauterine inflammation. A combination of antenatal inflammation and asphyxia increases the risk of cerebral palsy >70 times. The aim of the present study was to determine the effect of intracisternal (i.c.) administration of endotoxin [lipopolysaccharides (...
Chapter
Trotz weltweiter Anstrengungen ist die Frühgeburtlichkeit nach wie vor eines der herausragenden Themen der Perinatalmedizin. In Deutschland erleiden jährlich etwa 6—8% aller Schwangeren eine Frühgeburt. Obwohl die Anzahl der betroffenen Kinder zunächst gering erscheint, sind sie doch in hohem Maße von perinataler Mortalitätund Morbidität betroffen....
Article
Full-text available
Perinatal brain damage is associated not only with hypoxic-ischemic insults but also with intrauterine inflammation. A combination of antenatal inflammation and asphyxia increases the risk of cerebral palsy >70 times. The aim of the present study was to determine the effect of intracisternal (i.c.) administration of endotoxin [lipopolysaccharides (...
Poster
Full-text available
A MODEL OF NEONATAL CEREBRAL ISCHEMIA IN RATS (‘LEVINE’) TO STUDY NEUROPROTECTION AND NEUROREGENERATION J. MIDDELANIS1, H.-M. VAIHINGER1; Y. GARNIER1, K. GOTTMANN2, H. DINSE3, A. JENSEN1 1 Department of Obstetrics & Gynecology, University of Bochum 2 Institute of Cell Physiology, University of Bochum 3 Department of Neuroinformatics, University of...
Poster
Full-text available
CEREBRAL ISCHEMIA AND UMBILICAL STEM CELL TRANSPLANTATION IN CHRONICALLY PREPARED FETAL SHEEP Y. GARNIER1, H.-M. VAIHINGER1, J. MIDDELANIS1, O. BRÜSTLE2, A. JENSEN1 1Department of Obstetrics & Gynecology, University of Bochum 2Institute of Reconstructive Neurobiology, University of Bonn Objective: Hypoxic-ischemic cerebral damage is an important co...
Data
Full-text available
First International Meeting of the Stem Cell Network North Rhine Westphalia, Düsseldorf, November 26-27, 2002; 11/2002
Data
Full-text available
First International Meeting of the Stem Cell Network North Rhine Westphalia, Düsseldorf, November 26-27, 2002; 11/2002
Article
Full-text available
We investigated the effects of magnesium on metabolic disturbances in hippocampal slices prepared from fetal guinea pigs after oxygen-glucose deprivation (OGD). Metabolic disturbances were assessed by measuring changes in energy metabolism and protein synthesis. In addition we determined cyclic guanosine monophosphate (cGMP) concentrations in the s...
Article
Objective:We investigated the effects of magnesium on metabolic disturbances in hippocampal slices prepared from fetal guinea pigs after oxygen-glucose deprivation (OGD).

Citations

... The objective of the use of tocolytic is to delay the active phase of labor (9,10,17,18). Epidemiological documents have shown that treatment of mothers with magnesium sulfate would result in myocardial stability and blood supply in placenta and fetal brain (19), and reduction of ischemic region (20) and anti-oxidant effects with decreased platelet adhesion (21) are neuroprotective in fetus. Nevertheless, the results of studies are controversial. ...
... Administration of 2 million UCB mononuclear cells at 3 h after HI reduced neuronal degeneration and cas- pase-3 expression by 25% and, at 7 days, microglial activation was significantly reduced in the cortex [84]. UCB given 24-h post-neonatal HI injury normalized toe spread and forepaw symmetry, increased sensorimotor electrophysiology and decreased spastic paresis [84, 85]. Wang et al. [86] administered UCB cells directly into the ventricles, 24-h post-HI injury, and showed a decrease in neuronal loss in the cortex and CA1 region of the hippocampus. ...
... Spastic Paresis. First mention of the concept of amelioration of perinatal brain damage by neuroregeneration using cord blood dates back to 2002, when our preliminary data in chronically prepared fetal sheep showed a highly specific invasion of human umbilical cord blood mononuclear cells (huMNC) into the damaged brain region, that is, "homing, " in a model of cerebral ischemia [13]. This was the beginning of a series of experiments in vitro and in vivo, using a different animal model ("Levine") of perinatal cerebral hypoxic-ischemia in newborn rats to increase effectiveness [14,15]. ...
... First mention of the concept of amelioration of perinatal brain damage by neuroregeneration using cord blood dates back to 2002, when our preliminary data in chronically prepared fetal sheep showed a highly specific invasion of human umbilical cord blood mononuclear cells (huMNC) into the damaged brain region, that is, "homing, " in a model of cerebral ischemia [13]. This was the beginning of a series of experiments in vitro and in vivo, using a different animal model ("Levine") of perinatal cerebral hypoxic-ischemia in newborn rats to increase effectiveness [14,15]. The "Levine" model includes unilateral carotid artery ligation followed by 8% O 2 inhalation for 80 minutes on postnatal day (PN) 7. The neurodevelopment of the rat at PN 7 is equivalent to that of the human brain at birth [16]. ...
... In the past ten years, the combination of infection/inflammation plus hypoxia/ischemia (HI) has been reproduced experimentally in rats at early neurodevelopment stages in rat pups between P1 and P7 equivalent to early and late preterm development of human neonates [10][11][12][13][14]. Lipopolysaccharide (LPS) is an important component of Gram-negative bacteria and has been used to make animal models of inflammation. ...
... Estradiol (E2), as a cholesterol-derived estrogen steroid hormone, is involved in the regulation of reproductive functions (20) and can act as a neuroprotective agent in some brain injuries (21). In addition, the anti-apoptotic (22), antioxidant (23), and anti-inflammatory (24) effects of E2 have been reported based on experimental TBI. It has been proposed that treatment with estradiol after ischemia can reduce injury to the cerebral cortex (25). ...
... In vitro studies show that magnesium alleviates excitotoxic damage by binding to the magnesium site on the NMDA glutamate channel to reduce the accumulation of cytotoxic levels of intracellular calcium (68). In this setting, increased extracellular magnesium is associated with recovery of high energy phosphate stores, improved rates of protein synthesis and neural preservation in ischemic hippocampal neurons from fetal and adult rodents (69,70), and after global ischemia in adults when magnesium was given by direct intra-hippocampal injection (71). However, benefit required a 2-4-fold increase in extracellular magnesium concentrations. ...
... When LPS was injected mid-or full gestation into pregnant sheep, there were observable injuries to the fetal brain, particularly in the white matter [125][126][127][128]. Intracerebral LPS exposure showed increased pro-inflammatory markers and caused a loss of oligodendroglial precursors. Intracerebral LPS exposure was also implicated in loss of myelination and bilateral ventricular dilation [129][130][131]. In addition, Oskvig et al. showed that pregnant Sprague Dawley rats exposed or challenged with LPS showed an increase in maternal serum cytokines [132]. ...
... Early intervention of an infant with severe visual impairment caused by brain damage may have a different prognosis (Dutton and Jacobson, 2002). Difficulties that occur in children with perinatal brain damage may remain present for the rest of their lives (Jensen, Garnier, Middelanis, & Berger, 2003). ...
... Eight out of 17 studies were performed in perinatal models of HIE (Table 2). Six out of 8 studies were conducted in rodents between postnatal days (P)7 and P20 [18][19][20][21][22][23][24], which is considered comparable to human brain development between near-term/term and late infancy [25,26]. One study was conducted in term spiny mice which in terms of brain development are broadly comparable to the term human [27]. ...