January 2019
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9 Reads
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Publications (47)
January 2019
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16 Reads
January 2019
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126 Reads
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44 Citations
Background: During its persistence in cystic fibrosis (CF) airways, P. aeruginosa develops a series of phenotypic changes by the accumulation of pathoadaptive mutations. A better understanding of the role of these mutations in the adaptive process, with particular reference to the development of multidrug resistance (MDR), is essential for future development of novel therapeutic approaches, including the identification of new drug targets and the implementation of more efficient antibiotic therapy. Although several whole-genome sequencing studies on P. aeruginosa CF lineages have been published, the evolutionary trajectories in relation to the development of antimicrobial resistance remain mostly unexplored to date. In this study, we monitored the adaptive changes of P. aeruginosa during its microevolution in the CF airways to provide an innovative, genome-wide picture of mutations and persistent phenotypes and to point out potential novel mechanisms allowing survival in CF patients under antibiotic therapy. Results: We obtained whole genome sequences of 40 P. aeruginosa clinical CF strains isolated at Trentino Regional Support CF Centre (Rovereto, Italy) from a single CF patient over an 8-year period (2007–2014). Genotypic analysis of the P. aeruginosa isolates revealed a clonal population dominated by the Sequence Type 390 and three closely related variants, indicating that all members of the population likely belong to the same clonal lineage and evolved from a common ancestor. While the majority of early isolates were susceptible to most antibiotics tested, over time resistant phenotypes increased in the persistent population. Genomic analyses of the population indicated a correlation between the evolution of antibiotic resistance profiles and phylogenetic relationships, and a number of putative pathoadaptive variations were identified. Conclusion: This study provides valuable insights into the within-host adaptation and microevolution of P. aeruginosa in the CF lung and revealed the emergence of an MDR phenotype over time, which could not be comprehensively explained by the variations found in known resistance genes. Further investigations on uncharacterized variations disclosed in this study should help to increase our understanding of the development of MDR phenotype and the poor outcome of antibiotic therapies in many CF patients.
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Citations (5)
... and exhibit changes in metabolism, antibiotic resistance, motility and other virulence factors that could affect Pf infection (44,46,52). While the prevalence of Pf-infected bacteria increases with patients' age (9), the mechanisms driving this increase are unclear. ...
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January 2019
... Motility test of the isolates was done using hanging drop preparation [19]. It was then mounted on a microscope slide and examined using X10 and X40 objectives lens respectively to determine the presence or absence of motility and the results recorded. ...
- Citing Article
January 2010
La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine
... Period 2012 to date: In 2012 the program was partly redesigned under the guidance of another author of the present paper (F.M.), who is an expert in urinalysis [26][27][28][29], and in collaboration with the GIAU [30][31][32]. Images, proposed from the coordinator of the EQA program (S.S.), were chosen if there was 80% or greater consensus of referee laboratories, which are members of GIAU. ...
- Citing Article
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April 2011
Biochimica Clinica
... En la práctica clínica, el urianálisis se suele realizar ante la sospecha de infección del tracto urinario, para evaluación inicial o seguimiento de la enfermedad renal, de enfermedades no infecciosas del tracto urinario (primarias o causadas por patologías sistémicas, como enfermedades reumatológicas, hipertensión, otras patologías del embarazo o los efectos secundarios de tratamientos farmacológicos) y en el caso de formación recurrente de litiasis renal [3,5]. El análisis de orina también se suele solicitar en los servicios de urgencias, principalmente como herramienta de detección para evaluar estados de hidratación e hipovolemia (p.ej. ...
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September 2011
La Rivista Italiana della Medicina di Laboratorio - Italian Journal of Laboratory Medicine
... La stragrande maggioranza degli intervistati riconosce, quindi, la necessità di definire nella maniera più precisa possibile i criteri di allocazione dei pazienti alle diverse aree e in particolare all'area cosiddetta ad alta intensità da pronto soccorso e all'area post-acuti dalla degenza ''intermedia''. Tuttavia, la propensione a adottare criteri integrati di tipo clinico, anziché esclusivamente ''aridi'' score, nonché la possibilità adombrata da quasi la metà degli intervistati di estendere a tali aree l'ammissione di pazienti anche appartenenti a specialità non omogenee tra di loro, sottende una flessibilità che, evidentemente, deve andare incontro alle caratteristiche di ogni singolo ospedale [26]. Ospedali che, come sappiamo bene, si differenziano sensibilmente a seconda delle dimensioni e della collocazione geografica. ...
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