Michel DuBois's research while affiliated with Saint Catherine University and other places
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Publication (1)
Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orange-yellow color when treated with phenol and concentrated sulfuric acid. The reaction is sensitive and the color is stable. By use of this phenol-sulfuric acid reaction, a method has been de...
Citations
... The determination of total carbohydrates was carried out according to the suitably modified method proposed by Dubois et al. [19]. 2 mg of freeze-dried biomass were weighed inside an Eppendorf tube and suspended in 1000 μl of phosphate-buffered saline (PBS) (20 mM, pH 7.4). ...
