Michaela Weimer's research while affiliated with Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB and other places

Publications (10)

Article
This review article addresses the relevance and potential of bioartificial tissues in oncologic research and therapy and reconstructive oncologic surgery. In order to translate the findings from basic cellular research into clinical applications, cell-based models need to recapitulate both the 3 D-organization and multicellular complexity of an org...
Article
Full-text available
A formal validation study was performed, in order to investigate whether the commercially-available reconstructed human epidermis (RHE) models, EPISKIN, EpiDerm and SkinEthic, are suitable for in vitro skin absorption testing. The skin types currently recommended in the OECD Test Guideline 428, namely, ex vivo human epidermis and pig skin, were use...
Article
Full-text available
The 3-D skin equivalent can be viewed as physiologically comparable to the natural skin and therefore is a suitable alternative for animal testing. This highly differentiated in vitro human skin equivalent is used to assess the efficacy and mode of action of novel agents. This model is generated from primary human keratinocytes on a collagen substr...
Article
This review article addresses the relevance and potential of bioartificial tissues in oncologic research and therapy and reconstructive oncologic surgery. In order to translate the findings from basic cellular research into clinical applications, cell-based models need to recapitulate both the 3D organization and multicellular complexity of an orga...
Article
This review article addresses the relevance and potential of bioartificial tissues in oncologic research and therapy and reconstructive oncologic surgery. In order to translate the findings from basic cellular research into clinical applications, cell-based models need to recapitulate both the 3D organization and multicellular complexity of an orga...
Article
Full-text available
Monolayer and suspension cultures have limited predictive value for target screening especially for nanomaterials. Target screening requires testsystems that mimic the human cancer with increasing accuracy in order to optimize the selection of potential effectors. Tissue engineering is not only the generation of tissues it should also identify biol...

Citations

... More recently, this promising technology has been applied to the field of oncology with some attempts to develop engineered tumor tissues for pre-clinical research (e.g. human melanoma model) [78]. Here we took advantage of our tissue engineering know-how in the respiratory field [79] to develop a complex, but accessible, 3D lung cancer model: OncoCilAir™ [80,81]. ...
... Currently, RHEs meeting the specified morphological and functional criteria have been validated as in vitro stand-alone models for skin corrosion [24], skin irritation [25], and recently updated, EpiDerm™ for phototoxicity tests [26]. Pre-validation tests using other safety endpoints, for example, genotoxicity [27,28], skin sensitization [29] and percutaneous absorption [30], are also under evaluation for potential adoption into the OECD Guidelines. ...
... Relatively simple to complex skin models have been described which are grown under static culture conditions with the culture medium reaching the skin from underneath and the surface being exposed to air to promote optimal epidermal differentiation and the formation of a stratum corneum [4][5][6][7][8]. These models are starting to include the epidermis containing, for example, keratinocytes, melanocytes, Langerhans Cells, the dermis, which contains fibroblasts and endothelial cells, and, in a few cases, adipocytes [9][10][11][12][13]. ...
... The perfusion system provides intra luminal pulsatile flow ensuring the necessary shear stress for endothelial cells. A computer regulates the arterial oxygen and nutrient supply via parameters such as blood pressure, temperature, and flow rate235,256 . InFigure 3.1 B a photograph of the bioreactor system within the drawer of the incubator is depicted. ...