Michael R. Gretz's research while affiliated with Michigan Technological University and other places

Publications (53)

Article
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Avoidance response is a well-known mechanism for escaping environmental stress. For organisms with reduced active movement, such as benthic microalgae, drifting could be a specifically selected mean of avoiding less favorable environments. To test this hypothesis, a system was developed to assess if hypo-saline stress triggers drift in the estuarin...
Article
In wetland habitats, periphyton is a common component of open-water areas with species assemblage determined by local water quality. Extracellular polymeric substances (EPS) secreted by algae and bacteria give structure to periphyton, and differences in EPS chemistry affect the functional roles of these polymers. The Florida Everglades provide a un...
Article
Cell-wall (CW) development in the desmid Penium margaritaceum (Ehrenb.) Bréb. was studied using immunofluorescence labeling of living cells with the monoclonal antibodies (mAbs) JIM5 and JIM7, which recognize unesterified and methyl-esterified homogalacturonan (HG), respectively. During cell expansion, HG was secreted in a high-esterified form at a...
Article
Pennate diatoms are the main group of primary producers in transient microbial biofilms at the sediment surface in muddy intertidal systems. These microalgae produce a substantial quantity of extracellular polymeric substances (EPS) that contribute significantly to the cohesive properties observed in biofilms. Changes in carbohydrate content and co...
Article
Extracellular polymeric substance (EPS) secretion was examined in the stalked marine diatom Achnanthes longipes Ag. in defined medium. This common biofouling diatom exhibited an absolute requirement for bromide for stalk production and substratum attachment, whereas elevated iodide concentrations in the growth medium inhibited stalk formation and a...
Article
Desmids represent a group of advanced green algae that are commonly found in biofilm communities of freshwater wetlands. Desmids secrete significant amounts of extracellular polymeric substances (EPS) that form an extensive mucilaginous sheath external to the cell wall and function in adhesion, gliding-based movements and ultimate ensheathment with...
Article
Pleurotaenium trabecula (Ehren.) Nägeli is a placoderm desmid that commonly occurs in wetland biofilms of the southeastern Adirondacks (NY, USA). It often displays a distinctive habit whereby the cell remains attached to the substrate via the polar end of one semicell, while the remainder of the cell is suspended in the water column. In this study,...
Article
Plant cell walls are essential for proper growth, development, and interaction with the environment. It is generally accepted that land plants arose from aquatic ancestors which are sister groups to the charophycean algae (i.e., Streptophyta), and study of wall evolution during this transition promises insight into structure-function relationships...
Article
Cylindrotheca closterium (Ehrenberg) Reiman et Lewin is a raphid diatom widely distributed in mudflat assemblages. Video microscopy showed various movement modalities defined as smooth and corkscrew gliding, pirouette, pivot, rock and roll, rollover, and simultaneous pirouette and gliding. Z-axis projection analysis of images revealed a unique glid...
Article
The effects of phosphate (P) limitation, varying salinity (5–65 psu), and solid media growth conditions on the polysaccharides produced by the model diatom, Phaeodactylum tricornutum Bohlin were determined. Sequential extraction was used to separate polymers into colloidal (CL), colloidal extracellular polymeric substances (cEPS), hot water soluble...
Article
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We studied patterns of production and loss of four different extracellular polymeric substance (EPS) fractions— colloidal carbohydrates, colloidal EPS (cEPS), hot water (HW)–extracted and hot bicarbonate (HB)–extracted frac-tions—and community profiles of active (RNA) bacterial communities by use of Terminal-Restriction Fragment Length Polymorphism...
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Benthic microalgae (microphytobenthos) are the dominant group of primary producers in many marine intertidal and subtidal habitats. Estuarine mudflat diatoms are thought to be major contributors of extracellular polymeric substances (EPS), which are important for sediment stabilization and in benthic food chains. Biofilms from 6 sites in the Colne...
Article
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Diatom frustules have been identified as potential candidate materials for nanotechnology applications. However, for successful engineering applications, their mechanical properties must be fully determined. Toward this end, indentation hardness and elastic properties frustules of the centric diatom Coscinodiscus concinnus were evaluated using nano...
Article
Cell walls of the generic phase of the freshwater red alga Lemanea annulata Kütz were mechanically isolated and chemically characterized. Walls consisted mainly of polysaccharide with lesser quantities of associated protein and lipid. The major wall component was alkali-soluble xylan, comprised mainly of 4-linked β-xylopyranosyl residues and small...
Article
Extracellular matrix (ECM) polymers secreted by the diatoms Achnanthes longipes Ag. and Cymbella cistula (Ehr.) Kirchn. completely encase the cell and are responsible for adhesion and other interactions with the external environment. To preserve details of the highly hydrophilic ECM in the native state and to preserve, with a high degree of fidelit...
Article
Although diatom extracellular matricies are usually thought of exclusively in terms of the beautiful, architecturally complex silicious frustule, polymers exuded through the frustule are critical mediators of interactions with the external environment. In several species, complex proteoglycans appear to be the primary components involved in adhesio...
Article
Field emission scanning electron microscopy (FESEM) provides a direct view of a biological sample with high spacial resolution. Combining FESEM with cryo-techniques, macromolecular structures have been obtained from single SEM image successfully (Hermann & Müller, 1992; Chen et al. , 1995, 1997). This protocol is now applied to study the extracellu...
Article
The attachment of diatoms to surfaces is an important and poorly understood step in the development of biofouling communities. Experiments were performed in vitro on a common fouling diatom (Achnanthes longipes) to determine the influence of the base material and bacterial conditioning on diatom attachment. The first series of experiments compared...
Article
An integrated approach including TEM, SEM and LM was utilized to investigate adhesives produced by the marine fouling diatom Achnanthes longipes. Extracellular adhesive secretion during sequential attachment in living diatom cells was examined using video microscopy. A suite of cryotechniques including high pressure freezing (HPF), freeze-substitut...
Article
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Achnanthes longipes is a marine, biofouling diatom that adheres to surfaces via adhesive polymers extruded during motility or organized into structures called stalks that contain three distinct regions: the pad, shaft, and collar. Four monoclonal antibodies (AL.C1-AL.C4) and antibodies from two uncloned hybridomas (AL.E1 and AL.E2) were raised agai...
Article
The cell wall polysaccharides of two species of red algae, which are adapted to both freshwater and marine environments, were analysed to determine the effect of these widely different environments on their commercially important agarocolloids and to investigate the possible role of the cell wall in environmental adaptation. Cell wall polymers of f...
Article
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We have answered fundamental questions regarding the mechanisms of attachment and the nature of the biocomposite adhesives utilized by the marine biofouling diatom Achnanthes longipes and the freshwater Cymbella cistula. During the course of this grant we have: (1) delineated the sequence of events involved in attachment of the organisms to a varie...
Article
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Extracellular adhesives from the diatoms Achnanthes longipes, Amphora coffeaeformis, Cymbella cistula, and Cymbella mexicana were characterized by monosaccharide and methylation analysis, lectin-fluorescein isothiocyanate localization, and cytochemical staining. Polysaccharide was the major component of adhesives formed during cell motility, synthe...
Article
The cellulose synthesis inhibitor 2,6-dichlorobenzonitrile (DCB) and the DCB analogs 2-chloro-6-fluorobenzonitrile, 3-amino-2,6-dichlorobenzonitrile, and 5-dimethylamino-naphthalene-1-sulfonyl-(3-cyano-2, 4-dichloro)aniline (DCBF) inhibited extracellular adhesive production in the marine diatom Achnanthes longipes, resulting in a loss of motility a...
Article
Stable isotopes are preferable in many ways to radioactive isotopes for metabolic studies designed to elucidate biosynthetic pathways. We have developed the methodology to utilize 13C-labelled compounds in tracer studies of saccharide metabolism in the red algae. Cultures of Agardhiella subulata were pulse-chase labelled with 13C02 and 12C02. Gas c...
Article
Lipids were isolated from the elaiosomes of four ant-dispersed herbaceous plants (Jeffersonia diphylla, Sanguinaria canadensis, Trillium sessile, Dicentra cucullaria) found in Northern Virginia. Fatty acid methyl esters and trimethysilyl derivatives of diacylglycerols were separated and identified by capillary GC/MS. Oleic acid and palmitic acid ar...
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The two-layered, fibrillar cell wall of Mougeotia C. Agardh sp. consisted of 63.6% non-cellulosic carbohydrates and 13.4% cellulose. The orientation of cellulose microfibrils in the native cell wall agrees with the multinet growth hypothesis, which has been employed to explain the shift in microfibril orientation from transverse (inner wall) toward...
Article
L'exposition d'organismes synthetisant de la cellulose a un champ magnetique perturbe la biogenese de la cellulose. La modification de l'organisation des microfibrilles dans la structure de la cellulose est observee en microscopie electronique. Ce phenomene est mis en evidence chez une plante, Avena sativa et chez une bacterie, Acetobacter xylinum
Article
Mechanically isolated cell walls were prepared from the conchocelis phases of Bangia atropurpurea (Roth) C. Ag. and Porphyra leucosticta Thur. Electron microscopic observations of cell wall preparations revealed them to be morphologically similar to walls of whole thalli and free from protoplasmic contamination. Chemical analyses of isolated walls...
Article
Cellulose has been characterized from isolated cell walls of the conchocelis phases of both Porphyra umbilicalis and P. leucostricta. Evidence for cellulose II (regenerated cellulose) in Schweitzer's reagent extracts was provided by X-ray powder analysis and paper chromatography of partial hydrolyzates. The presence of cellulose in the conchocelis...
Article
Thirty years ago Smith & Cook (1953) published the first account of fractionation of carrageenan from the red alga Chondrus crispus, and introduced the terms κ- and λ-carrageenans to indicate KClinsoluble and -soluble polysaccharides. Ten years later Rees (1963) published the first in a series of papers entitled ‘The carrageenan system of polysacch...
Article
Galactan sulphates have been extracted from mechanically isolated cell walls of laboratory cultured conchocelis phases of Porphyra leucostricta and Bangia atropurpurea and their structure established by chemical methods and 13C-n.m.r. spectroscopy. The polysaccharides represent an extreme structure for porphyran-type polymers in that they consist a...
Article
Mechanically isolated cell walls of the conchocelis phase of Bangia fuscopurpurea yield cellulose II (regenerated cellulose) upon treatment with Schweitzer's reagent. X-ray powder analysis and thin-layer chromatography of partial hydrolyzates confirm the presence of cellulose in this extract. Gas-liquid chromatographic analysis of wall hydrolyzates...
Article
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Electron microscopy of field-collected vegetative cells of Schizochlamys gelatinosa revealed a pyrenoid extending from the inner surface of the chloroplast. The entire structure is enclosed by a cap, differing completely from the types of pyrenoids previously described for the green algae. Schizochlamys is placed in the family Schizochlamidaceae n....
Article
Full-text available
This cooperative Augmentation Award for Science and Engineering Research Training has provided training for two doctoral students in several important areas of marine microalgal biofouling, including marine diatom mass culturing and adhesive isolation, biochemistry of adhesives, and laboratory experiments on the effects of substrate preconditioning...

Citations

... The flagella of the green alga Chlamydomonas have been used as a model of flagellar structure. Flagella structure has been highly conserved throughout evolution, images from Chlamydomonas are virtually indistinguishable from flagella (or cilia -a term for a short flagellum) of mammalian cells including human sperm and certain epithelia (Johnson, 1995). Chlamydo monas has been chosen because of the ease of growing the organism and because the flagella can be detached from the cells by pH shock or blending. ...
... The most common form of motility in pennate diatoms is known as "gliding," consisting in directed cell movement, typically along the direction parallel to the longitudinal axis of the cell, and when the cell is in close contact with hard surfaces (Edgar and Pickett-Heaps 1984). Directed motility, for example, toward a stimulus, is achieved by varying the time between the reversal of direction, causing forward progression when the movement in the direction of the stimulus lasts longer than away from it (Cohn et al. 2004;Apoya-Horton et al. 2006). Diatom gliding is a complex process, involving the extrusion of adhesive, mucilaginous extracellular polymeric substances (EPS) through the raphe. ...
... Considering the characteristics described above, the production and application of original polysaccharides as therapeutic agents have become increasingly important topics of research. Unfortunately, the SPs of green seaweeds are structurally diverse and heterogeneous [31,32], which makes studies on their structures challenging and hinders their development as therapeutic agents [33]. The production of a standardized commercial product based on green algal SP constituents is expected to be a significant endeavor because the structural and pharmacological features of these components may vary according to the species and location and time of harvest [34][35][36][37][38]. ...
... It produced negative ecological and economic consequences (Alpert et al. 2000;Beville et al. 2012;Taylor and Bothwell 2014). Considerable attention has been paid to this D. geminata problem, as it has invaded more than 187 rivers in many other countries (Blanco and Ector 2009;Gretz et al. 2007). This benthic diatom has been declaring an invasive species in Southern Hemisphere countries such as New Zealand (Kilroy and Unwin 2011), Argentina and Chile (Reid and Torres 2014) since 2010 (Segura 2011). ...
... Bourrelly 1966;Fott 1972;Prescott 1962;Smith 1950). In a strain of S. gelatinosa isolated from Kansas, USA, Wujek (1968) and Wujek & Gretz (1977) described vague pseudoflagella and stalked, oblong, bulb-shaped pyrenoids in cup-shaped chloroplasts in multinucleate vegetative cells. My observations on unauthentic S. gelatinosa SAG 66.94 did not agree with most features described in the literature. ...
... During the period 1982-1986 alone there were 50 new species and varieties of Mallomonas discovered and described in the journal literature (Asmund, Cronberg & D/irrschmidt, 1982;Kristiansen, 1982;Nicholls, 1982Nicholls, , 1984bNicholls, , 1987aD/irrschmidt 1982a, b, 1983a, b, c, 1984Croome & Tyler, 1983a, b, c;Wujek 1984;Cronberg & Hickel, 1985;Dfirrschmidt & Croome, 1985;Gretz, Sommerfeld & Wujek, 1985;Cronberg, 1986). This paper describes three new members of the genus Mallomonas, recently discovered in Ontario, Canada. ...
... These results can be an asset to improve risk assessment frameworks for salinized freshwater environments, considering that most of the prevention measures are no longer suitable, so directional mitigation or restoration actions can be complemented and/or substantially enriched by the results presented here. Adeyemi and Klerks, 2013;Araújo et al., 2012;Araújo et al., 2013;Fogels and Sprague, 1977;Griffith, 2017;Guan and Wang, 2006;Hintz et al., 2019;Lopes et al., 2004;Lopes et al., 2005;Moreira-Santos et al., 2008;Muyssen and Janssen, 2004;Oliveira-Filho et al., 2004;Osundeko et al., 2014;Shaw et al., 2019;Vousdoukas et al., 2020;Yan et al., 2015. ...
... Elaiosome lipid chemistry has been well described (Boulay, Coll-Toledano, & Cerdá, 2006; Boulay, Coll-Toledano, Manzaneda, & Cerdá, 2007;Bresinsky, 1963;Brew et al., 1989;Fischer et al., 2008;Gammans, Bullock, Gibbons, & Schönrogge, 2006;Hughes & Westoby, 1994;Kusmenoglu, Rockwood, & Gretz, 1989;Lanza, Schmitt, & Awad, 1992;Leal et al., 2014;Lisci et al., 1996;Skidmore & Heithaus, 1988;Wu, Peng, Dong, Xia, & Zhao, 2019) and is known to influence both the quantitative and qualitative aspects of seed dispersal effectiveness (sensu Schupp, 1993;Schupp et al., 2010) by ants. Seed-dispersing ants display a preference for food items containing high concentrations of oleic acid, an important signaling compound that stimulates foraging behavior, and thus select for seeds containing high concentrations of this compound (Boulay et al., 2006(Boulay et al., , 2007Brew et al., 1989;Fischer et al., 2008;Lanza et al., 1992;Pfeiffer, Huttenlocher, & Ayasse, 2010;Turner & Frederickson, 2013). ...
... Additionally, the growth phase influences EPS composition for marine diatoms (Penna et al., 1999) and the red microalga Porphyridium purpureum (Li et al., 2020), highlighting the flexibility of the EPSrelated pathways (Aslam et al., 2018). In the case of Phaeodactylum tricornutum, the EPS monosaccharide composition can change depending on the physiological state of the diatom (Willis et al., 2013) and growth phase (Bellinger et al., 2005;Underwood et al., 2004). Our results suggest that P. tricornutum modulates its polysaccharide biosynthesis machinery to adapt to the different cultural conditions, thereby influencing its bioactivity. ...
... The modern concept that all red algae should be grouped into a single class is supported by the occurrence of cellulose in the conchocelis phases of Bangia atropurpurea as well as Porphyra tenera (Gretz et al., 1982). Cellulose was also later found in the conchocelis phases of Porphyra leucostica and Porphyra umbilicalis; the quantities reported were 3.8%, 5%, and 11.8% for the Porphyra species investigated (Gretz et al., 1986;Mukai et al., 1981). It may be concluded that the cellulose from red algae is present in much lower amounts and is less crystalline than from other sources. ...