Michael C Hill's research while affiliated with Case Western Reserve University and other places

... A gradient of complexes PEI/siRNA and a spatial regulation of the GFP expression were achieved along the hydrogel, exhibiting the potential of these systems in organ bioengineering. 163 DEX hydrogels can also be prepared by cross-linking the chains through a Michael addition reaction between thiol groups and vinyl sulfones or acrylates. This type of reaction happens under physiological conditions, preventing the degradation of sensitive biomolecules. ...

... An in vitro degradation profile of BCP-6Sr2Mg2Zn, BCP-6Sr2Mg2Zn-PEU and BCP-6Sr2Mg2Zn-PCL scaffolds (~10 mm × 10 mm) with an average thickness of 2 mm was evaluated in α-MEM medium without ribonucleosides and deoxyribonucleosides (GIBCO™ Invitrogen Corporation, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Cytiva HyClone™ Fetal Bovine Serum (FBS) U.S. Origin, Fisher Scientific, Loughborough, UK), 1% Penicillin/Streptomycin and 1% Amphotericin B (Gibco) at pH = 7.4, according to the method previously used by Jeong and co-workers [41]. Briefly, the dried scaffolds were immersed in the culture medium at a 1:10 ratio of scaffold weight (g) to solution volume (mL) in a constant temperature incubator shaker (37 • C, 100 rpm). ...