Melissa G. Steiner's research while affiliated with Cornell University and other places

Publications (10)

Article
The pathobiology of salivary neoplasms can best be studied in a model system that reflects the native state of the tumor. The present study describes the use of a three-dimensional collagen gel (organoid) system in which pleomorphic adenomas of the parotid gland were propagated in vitro. Five pleomorphic adenoma cultures were established as organoi...
Article
In the adult epidermis, keratinocytes do not normally express the type-1 inhibitor of plasminogen activator (PAI-1). Basal epithelial cell-specific PAI-1 synthesis, however, accompanies epidermal wound repair in vivo in which PAI-1 transcripts and immunoreactive protein are confined to epithelial cells in the migrating tongue and the hyperprolifera...
Article
The pathobiology of salivary neoplasms can best be studied in a model system that reflects the native state of the tumor. The present study describes the use of a three-dimensional collagen gel (organoid) system in which pleomorphic adenomas of the parotid gland were propagated in vitro. Five pleomorphic adenoma cultures were established as organoi...
Article
The hypothesis tested in this article is that if cholesteatomas are a low-grade squamous cell neoplasm, then evidence of genetic instability, in the form of abnormal or aneuploid amounts of DNA, should be evident. Cholesteatoma is a destructive lesion of the middle ear and/or mastoid process that produces complications by erosion of the temporal bo...
Article
v-Crk is a member of a class of SH2 and SH3-containing adaptor proteins that have been implicated in regulating the TrkA receptor tyrosine kinase and potentiating Nerve Growth Factor (NGF)-mediated neurite outgrowth in pheochromocytoma (PC12) cells (Hempstead et al, Mol. Cell Biol. 14: 1964 - 1971). Given the fact that NGF induces both differentiat...
Article
Growth of human keratinocytes (NHKs) in submerged cultures approximates a "wound" response generally considered equivalent to regenerative maturation. Within this context, PAI-1 expression in cultured NHKs appears to be growth state-regulated and is associated with specific NHK subpopulations undergoing migration and proliferation in response to wo...

Citations

... The role of PAI-1 in fibroblast senescence has, however, not been investigated in these studies. This is important, since other cells, including keratinocytes, also produce PAI-1 during wound healing, and different cell types may be differently affected by loss of PAI-1 (Romer et al., 1991;Staiano-Coico et al., 1996). The identification of PAI-1 as an Nrf2 target gene raises the intriguing possibility that the positive effect of Nrf2-activating compounds or Keap-1 knockdown on wound healing in diabetic mice (Long et al., 2016;Rabbani et al., 2017;Soares et al., 2016) could be mediated in part via PAI-1 upregulation in fibroblasts. ...
... Cholesteatoma is a non-neoplastic lesion with a normal deoxyribonucleic acid (DNA) content (Desloge et al. 1997) and no inherent genetic instability is thought to be connected to the disease (Albino et al. 1998). Thus, heredity has not been thought to be involved in the pathogenesis of cholesteatoma, but family clusters have been demonstrated (Homøe andRosborg 2007, Prinsley 2009). ...
... The discussion shall be limited to secretion of organic products, in view of the fact that little or no work has been so far directed to an examination of fluid secretion in salivary tumors. Tissue cultures of PA [35,36] and enzymatic dispersion of salivary tissue are, however, possible. Cellular preparations thus obtainable could be examined by means of electrophysiological techniques and microfluorimetry, to assess whether responses to neurohormonal stimuli and signalling processes are affected in salivary neoplasia. ...
... PA-I contributes to control the synthesis of the ECM and is induced upon wounding and has a profound influence on ECM remodeling by blocking proteolytical collagen degradation [57]. PA-I also facilitates the migration of keratinocytes during wound healing and promotes epidermal injury repair [58][59][60]. PAI-1 is abundantly expressed by fibroblasts and its gene induction by PRGF suggests a regulative effect of PRGF on ECM remodeling during wound healing. ...
... Increases in sphingomyelin hydrolysis are associated with differentiation following the activation of p75 NTR , while the TrkA receptor has been reported to have an antagonistic effect [10,23]. PC12 cells that overexpress the TrkA receptor produce neuronal characteristics faster than wild-type [24], suggesting that the ceramide generated from sphingomyelin hydrolysis is not essential to neuronal differentiation. Because of the complicated and sometimes contradictory observations, we elected to explore the extent to which ceramide derived from neosynthesis and from hydrolysis determined the differentiation or death of PC12 and TrkA cells. ...