Mehrnaz Katouzian-Safadi's research while affiliated with French National Centre for Scientific Research and other places
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Publications (11)
This study compares the photo- and radiosensitizing effect of 5-bromouracil (BrU) on breakage of double stranded DNA. Upon U.V. irradiation, we observe a hot spot of breakage at the nucleotide in 5' from the BrU. Upon X irradiation, the breakage probability at the same site is only slightly enhanced. This result shows that the radiosensitizing effe...
Photochemical induced cross-links between protein and nucleic acids are useful tools in the study of the protein-DNA interactions. The substitution of thymine by 5-bromouracil in DNA increases the photocross-linking yield, and reduces the direct damages to both DNA and proteins. Using the lac repressor-DNA non-specific interaction system, we have d...
Adenosine cyclic 3',5'-phosphate receptor protein (CRP or CAP) is a regulatory protein involved in the transcription of several operons in Escherichia coli. cAMP-independent, nonspecific complexes of CRP and DNA were investigated by photochemical cross-linking of the protein to nonspecific DNA, whose thymines are substituted by 5-bromouracil (BrUra...
Protein MC1 is the major chromosomal protein in methanosarcinaceae. Using photochemical crosslinking on 5-bromouracil-substituted
DNA, we identified the region of the protein that interacts with it. This region is located in the C-terminal part of the
polypeptlde chain, and the crosslinked amino-aclds are in the region 74–86. Tryptophan 74 is one o...
Cyclic AMP receptor protein (CRP) is a regulatory protein implicated in the transcription of several operons in Escherichia coli. Its activity is modulated by effectors, such as cAMP or cGMP, which could induce (or not) structural changes in the protein, and activate (or not) the transcription. CRP can bind non-specifically to DNA, and we investiga...
Abstract— Cyclic AMP receptor protein (CRP) is a regulatory protein implicated in the transcription of several operons in Escherichia coli. Its activity is modulated by effectors, such as cAMP or cGMP, which could induce (or not) structural changes in the protein, and activate (or not) the transcription. CRP can bind non-specifically to DNA, and we...
4',6-Diamidino-2-phenylindole, DAPI, forms fluorescent complexes with DNA. This property has been used to quantify DNA on the basis of fluorometric test. However, the fluorescence quantum yield of DAPI increases also with tRNA. DNA estimation needs particular care in the presence of tRNA. For DNA containing 50% adenine-thymine (AT), DAPI can be use...
We examined the circular dichroism spectra of intact Turnip yellow mosaic virus, freezed/thawed virus, empty capsid particles, and phenol extracted RNA. The circular dichroism signal of the empty capsid was found to contribute for less than 1% to the circular dichroism of the virus. Differences in the circular dichroism spectra indicate that TYMV-R...
Turnip yellow mosaic virus (TYMV) RNA escapes from viral capsids after freezing and thawing the virus, and the remaining capsids look very similar to natural capsids in the electron microscope after negative staining [Katouzian-Safadi, M., Favre, A., and Haenni, A. L. (1980) Eur. J. Biochem. 112, 478-486]. In order to understand how an RNA of 2 X 1...
The uncoating of turnip yellow mosaic virus in vitro induced by freezing and thawing has been investigated using a variety of biochemical techniques including the aminoacylation capacity of the viral RNA and the ability of the RNA to stimulate protein synthesis, as well as physico-chemical techniques such as sucrose gradient centrifugation and elec...
Citations
... Belladonna mottle virus (BDMV; Moline & Fries, 1974) belongs to the tymovirus group of monopartite 0108-7681/90/040562-06503.00 RNA plant viruses. The type member of this group, turnip yellow mosaic virus (TYMV) has been extensively studied in terms of the forces stabilizing the particle structure (Kaper, 1971(Kaper, , 1975Jacrot, Chauvin & Witz, 1977;Katouzian-Safadi, Favre & Haenni, 1980;Katouzian-Safadi & Berthet-Colominas, 1983;Katouzian-Safadi & Haenni, 1986;Keeling & Matthews, 1982). The integrity of the particle structure in these viruses is derived from strong hydrophobic association of protein subunits. ...
... Considering that Shetlar et al. (1984) demonstrated a high reactivity of tryptophan residue towards DNA upon UV-irradiation, and Zubarev et al. (1999) found that tryptophan residues have a very high reactivity towards radicals when compared to the other natural amino acid residues, one should expect that tryptophan would be a common cross-linking partner for DNA probes. One possible explanation for the rare observation of tryptophan cross-links was provided by Safadi et al. (1991a), who reported photochemical degradation of tryptophan residues upon prolonged UV-irradiation at 300 to 400 nm. In an earlier SSB–DNA UV-cross-linking study Phe-60 was identified as the site of cross-linking, which was not identified in our study (Merrill et al. 1984 ). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/715361028685826-1547566768654_Q64/Madia-Charlier-2.jpg)
... This is in agreement with Phe and Tyr frequently being found as RNA-interacting residues in RRMs (Clery, Blatter et al., 2008, Hobor, Pergoli et al., 2011, Teplova, Song et al., 2010, Tsuda, Kuwasako et al., 2009. As a result, we used these seven amino acids together with previously described tryptophan (Bley et al., 2011, Katouzian-Safadi, Laine et al., 1991, Reeve & Hopkins, 1980 to define new modifications in the MaxQuant search engine (Cox & Mann, 2008). All of the acquired data was subjected to large-scale data analysis using two different search engine platforms; either MaxQuant or using an in-house developed, Xi database search platform (ERI, Edinburgh) with a target modification search option (xiTMS) (see Methods and Supplemental Information). ...
... nucleic acids, 17,18 polyphosphate moieties, [19][20][21] and tubulin. 22 Such binding increases the fluorescence intensity or the peak shift of fluorescence. ...
Reference: Li J Histochem Cytochem.
... During natural infection of plants by TYMV, two types of virus particles are produced: normal virus with packaged RNA genome and empty particles known as natural top components (van Roon et al. 2004). The genomic contents within TYMV can be removed and converted to pot-like empty particles known as artificial top components (ATCs) through repeated free-thaw cycles (Katouzian-Safadi and Berthet-Colominas, 1983), high pressure (Leimk€ uhler et al. 2001) or alkaline treatment (Keeling and Matthews, 1982), where such ATCs have been deployed as a model to deliver fluorescein dye into baby hamster kidney cells through conjugation with transactivating transcriptional activator (TAT), a cell-penetrating peptide (CPP) (Kim et al. 2018). ...
... In the two decades that followed, scientific research mainly aimed at preventing or disintegrating viral aggregates to increase the infectious titer of laboratory-grown viral strains, minimize their batch-tobatch variations, and enhance the efficiency of virus neutralization in vitro and disinfection processes in the environment. These studies investigated the physicochemical parameters influencing viral aggregation and subsequently underscored its role in viral transport, adsorption, and retention, mainly in water bodies and in vitro settings [5,[20][21][22][23][24][25][26][27]. We have summarized them in the following two sections of this review. ...
Reference: Viral Aggregation: The Knowns and Unknowns
... 2. Photocross-linking yields have been re-volving DNA. Willis et al., 1994;Liu et al., 1994;Allen et al., 1991 ;Dietz and Koch, 1987;Ito et al., 1980;Katouzian-Safadi and Charlier, 1994;Dietz and Koch, 1989Weintraub, 1973Blatter et at., 1992Dietz and Koch, 1987Hicke et al., 1994 Dietz andKoch, 1987;Hicke et al., 1994 ...
... The interactions between CRP and DNA have an electrostatic character. In their complex, the positively charged protein fragments, from both the C-as well as the N-terminal domain of CRP, are involved [Katouzian-Safadi et al., 1993]. ...
