Meenu Tomer’s research while affiliated with Alzheimer’s Drug Discovery Foundation and other places

What is this page?


This page lists works of an author who doesn't have a ResearchGate profile or hasn't added the works to their profile yet. It is automatically generated from public (personal) data to further our legitimate goal of comprehensive and accurate scientific recordkeeping. If you are this author and want this page removed, please let us know.

Publications (22)


Identification and Quantitation of Signature Constituents of Dant‐Kanti, a Herbo‐Dental Formulation Using UHPLC, HPTLC, and GC–MS, and its Anti‐Inflammatory Effects on Porphyromonas gingivalis Lipopolysaccharide‐Induced Human Macrophages
  • Article

December 2024

·

14 Reads

Separation Science Plus

Acharya Balkrishna

·

Priya Rani Maheswari

·

·

[...]

·

A herbo‐dental formulation, Dant‐Kanti natural toothpaste consists of 13 potent medicinal herbs. The present study aims to comprehensively identify the signature phytometabolites present in Dant‐Kanti natural toothpaste using high‐performance thin layer chromatography (HPTLC), ultra‐high‐performance liquid chromatography (UHPLC), and gas chromatography–mass spectrometry (GC–MS) methods. Among the identified phytometabolites, gallic acid, piperine, and anethole, were quantified by UHPLC, whereas camphor, menthol, thymol, and eugenol were quantified using GC–MS. An inductively coupled plasma‐mass spectrometry was used to estimate the mineral contents in Dant‐Kanti natural toothpaste. To the best of our knowledge, these studies are the first‐ever to identify all the signature components in an herbal dental formulation. These developed and validated methodologies were then used across 25 distinct batches of Dant‐Kanti natural toothpaste, in oder to ascertain the consistency and homogeneity. Both the UHPLC, and GC–MS methods used in this study were validated as per ICH Q2 (R1) guidelines, and were found to be suitable, linear ( r > 0.99), and precise. In the cell‐biological study, Dant‐Kanti natural toothpaste showed a concentration‐dependent reduction in proinflammatory cytokine release, in human macrophages induced with Porphyromonas gingivalis lipopolysaccharide. This robust study methodology, validation, and results provide valuable insights into the complex composition of Dant‐Kanti natural toothpaste, and contribute to the understanding of its potential oral health benefits.


Co-administration of Ayurvedic medicines Arshogrit and Jatyadi Ghrit, attenuate croton oil-induced hemorrhoids in rat model of recto-anal inflammation by modulating TNF-α and IL-1β levels

November 2024

·

15 Reads

Objective: To study the efficacy of co-administration of Arshogrit (AG) and Jatyadi Ghrit (JG), two herb-based Ayurvedic medicines, in rat model of croton oil-induced hemorrhoids. Significance: Hemorrhoids refer to a pathological condition affecting the recto-anal region causing pain, swelling, bleeding and protrusion. The available therapies for hemorrhoids are symptomatic or invasive but are expensive and associated with adverse effects. Hence, there exists a need for efficacious and safer pharmacotherapies. Methods: Ultra high performance liquid chromatography detected nine phytocompounds in AG and seven in JG. Seven fatty acids were additionally identified in JG by Gas Chromatography-Mass Spectrometry analysis. The in-vivo efficacy of the co-administration of AG, which was administered orally at the doses of 20, 60 and 200 mg/kg/day and JG, which was topically applied (100 mg/animal/day) was evaluated in Wistar rats by inducing hemorrhoids development with the application of croton oil preparation (COP) in the recto-anal area. Prednisolone was employed as the standard drug and was administered orally at the dose of 1 mg/kg/day. Results: AG and JG were able to attenuate the croton oil-induced macro and microscopic anomalies. Gross pathological observation demonstrated remarkable decrease in croton oil-induced swelling, hemorrhage and formation of pseudomembrane, with the escalating doses of AG. Microscopic observation revealed alleviation in the histopathological lesions (necrosis, inflammation, hemorrhage/congestion, degeneration and dilatation of blood vessels). AG and JG additionally reduced COP-induced increase in the serum levels of pro-inflammatory cytokines. Conclusion: This study convincingly demonstrates that co-administration of AG and JG is a potential therapy against hemorrhoids, warranting further investigations.


Chemical characterization of Livogrit Vital (LVV) by HPLC. A Overlap chromatogram of standard mix (blue line) and Livogrit Vital (pink line) at 270 and 325 nm wavelength. B Overlap chromatogram of standard mix and LVV at 242, 283, and 325 nm wavelength. The quantification of the analysed phytometabolites is mentioned in Table 2
Livogrit Vital (LVV) decreased isoniazid-induced cytotoxicity in HepG2 cells. A Cytosafety of LVV (10, 30, and 100 µg/ml) and Silymarin (10 µM) in HepG2 cells. B Cytosafety of isoniazid (20–100 mM) in HepG2 cells. C Cytosafety of isoniazid (60 mM) induced HepG2 cells in presence of LVV (10, 30, and 100 µg/ml) and Silymarin (10 µM). D LVV shifted the IC50 of HepG2 cells induced with Isoniazid (20–100 mM). Data represented as mean ± S.E.M (n = 3). *, # (p < 0.05); ** (p < 0.01) and ***, ### (p < 0.001)
Livogrit Vital (LVV) decreased oxidative stress in isoniazid-induced HepG2 cells. LVV reduced (A) ROS levels, enhanced (B) GSH levels and also decreased (C) MDA levels in isoniazid (60 mM) induced HepG2 cells. Data represented as mean ± S.E.M (n = 3). **, ## (p < 0.01) and ***, ### (p < 0.001)
Livogrit Vital (LVV) decreased markers of ER stress and apoptosis in isoniazid-induced HepG2 cells. LVV treatment modulated the levels of (A) caspase-3, (B) FGF21, and (C) IRE-1α genes involved in modulation ER stress and apoptosis. Livogrit Vital treatment reduced the (D) caspase-3 activity and decreased protein levels of (E) cPARP1 in isoniazid-induced HepG2 cells. Data represented as mean ± S.E.M (n = 3). * (p < 0.05); ** (p < 0.01), and ***, ### (p < 0.001)
Livogrit Vital (LVV) decreased intracellular accumulation of isoniazid and hydrazine in HepG2 cells. A Chromatogram of HPLC based quantification of isoniazid levels. B Reference standard-based quantification of isoniazid as deciphered from chromatogram after isoniazid (60 mM) induction and treatment with LVV (30 and 100 µg/ml) of HepG2 cells. C Chromatogram of HPLC based quantification of hydrazine levels. D Reference standard-based quantification of hydrazine as deciphered from chromatogram after isoniazid (60 mM) induction and treatment with LVV (30 and 100 µg/ml) of HepG2 cells. E ALT and (F) GGT levels released from isoniazid (60 mM) induced and LVV (30 and 100 µg/ml) treated HepG2 cells. Data represented as mean ± S.E.M (n = 3). * (p < 0.05); ** (p < 0.01), and ***, ### (p < 0.001)
Herbo-vitamin medicine Livogrit Vital ameliorates isoniazid induced liver injury (IILI) in human liver (HepG2) cells by decreasing isoniazid accumulation and oxidative stress driven hepatotoxicity
  • Article
  • Full-text available

November 2024

·

21 Reads

BMC Complementary Medicine and Therapies

Background Tuberculosis (TB) is a leading cause of infection related mortality. Isoniazid is one of the frontline drugs for anti-TB therapy. Hepatotoxicity induced by isoniazid is a major cause of drug-discontinuation which may lead to development of resistant TB or increased mortality. Purpose To characterize pharmacological properties of plant-based prescription medicine, Livogrit Vital (LVV) against isoniazid-induced liver injury (IILI) using HepG2 cells. Method Phytometabolite characterization of LVV was performed by High-performance liquid chromatography (HPLC). The effects of LVV on cytosafety, IC50 shift, oxidative stress, ER stress, apoptosis, liver injury markers, and accumulation of isoniazid and hydrazine was performed on HepG2 cells induced with isoniazid. Silymarin was used as the positive control. Results HPLC based phytometabolite characterization of LVV revealed the presence of several anti-oxidant, anti-apoptotic, and hepatoprotective compounds. In isoniazid-induced HepG2 cells, LVV reduced cytotoxicity of isoniazid and shifted its IC50 value. Treatment with LVV reduced ROS generation and lipid peroxidation; enhanced GSH enzyme levels in isoniazid-induced HepG2 cells. As per the mechanistic evaluation, LVV modulated gene expression level of Caspase-3, FGF21, and IRE-1α. LVV treatment also normalized isoniazid-induced elevated Caspase-3 activity and cPARP1 protein levels, indicating its potentials to regulate liver cell apoptosis. Concomitantly, biomarkers of hepatotoxicity, ALT and GGT, also decreased by LVV treatment. Interestingly, LVV treatment reduced intracellular accumulation of isoniazid and its toxic metabolite hydrazine, in isoniazid-stimulated HepG2 cells. Conclusion Treatment of hepatic cells with the herbo-vitamin medicine, Livogrit Vital, regulates IILI by modulation of oxidative and ER stress, apoptosis, and bioaccumulation of isoniazid and hydrazine. Collectively, Livogrit Vital could well be explored as an adjuvant hepatoprotective agent alongwith anti-TB medicines.

Download

Comprehensive phytochemical profiling of Phyllanthus emblica L. flowers on UHPLC/MS quadrupole time of flight, HPTLC, HPLC, and NMR analytical platforms reveals functional metabolites with potent anti-inflammatory effects in human (THP-1) macrophages

August 2024

·

12 Reads

Phytochemical Analysis

Introduction Phyllanthus emblica L., renowned for its pharmacological benefits found in its fruits and leaves, has received considerable attention. However, there is a notable lack of research on its flowers, specifically on metabolite profiling and pharmacological activity. Objective The present study aims to delineate the phytochemical constituents of hydromethanolic extract of P. emblica flowers by ultra‐high‐performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC‐QToF‐MS), high‐performance thin layer chromatography (HPTLC), high‐performance liquid chromatography (HPLC), infrared and nuclear magnetic resonance spectroscopic methods and subsequent evaluation of its anti‐inflammatory potential. Materials and Methods The identification and characterization of phytochemicals in P. emblica flowers was performed by UHPLC/MS‐QToF in both positive and negative ionization modes. Additionally, marker compounds present in flower extract were analyzed using HPTLC, HPLC, FT‐IR, and NMR methods. The anti‐inflammatory potential was evaluated in lipopolysaccharide‐stimulated THP‐1 macrophages by evaluating inflammatory biomarkers. Results UHPLC/MS‐QToF analysis facilitated the identification of 51 compounds from P. emblica flowers including gallic acid derivatives, flavonoid glycosides, and tannins based on their fragmentation patterns and previous literature reports. Notably, the study also identified spermidine compounds for the first time in this species. Optimization of HPTLC and HPLC methods marked the presence of corilagin as major compound followed by FT‐IR and NMR spectral methods. Moreover, treatment with hydromethanolic extract of P. emblica flowers resulted in decreased levels of proinflammatory cytokines, TNF‐α, IL‐1β, and IL‐6, alongside modulation of nuclear factor‐κB activity in lipopolysaccharide‐induced THP‐1 macrophages. Conclusion Chromatographic techniques in conjunction with spectral methods found robust prevalence in the identification of signature phytometabolites present in P. emblica flowers, which sets the basis for its anti‐inflammatory potentials. The studies established a foundation for further exploration of potential applications of P. emblica flowers across various domains.


Schematic representation of establishment of endotoxin (LPS)‐induced fever model in Wistar rats. Following quarantine and acclimatization, experimental rats were randomly assigned to seven different groups namely, normal control (NC), disease control (DC), paracetamol (PCM‐200), FEVO‐20, FEVO‐60, FEVO‐200 and FEVO‐600. Animals were orally administered methylcellulose (NC and DC), paracetamol (100 mg/kg, b.i.d.) and four different doses of Fevogrit (10, 30, 100, and 300 mg/kg, b.i.d). One hour after administration of the compounds, the rectal temperature of animals was recorded and an intraperitoneal injection of LPS (50 μg/kg) was administered to animals in all groups except NC, which was injected with an equal volume of normal saline. Rectal temperature was recorded at 2, 4, 6, 12 and 24 h post‐LPS stimulation. At the 6‐h time point, the serum of the animals was collected for measurement of pro‐inflammatory cytokines. At 24 h post‐LPS administration, the hypothalamus of all animals was harvested for estimation of mRNA expression.
Phytometabolite analysis of Fevogrit by UHPLC. Fevogrit powder (pink line in chromatogram) was analyzed by ultra‐high‐performance liquid chromatography (UHPLC), along with a reference standard mix (chromatogram in blue line). The chromatographic profile of Fevogrit was developed at a wavelength of 270 nm. UHPLC analysis detected the presence of six marker bioactive compounds, namely, picroside II, picroside I, vanillic acid, cinnamic acid, magnoflorine and cordifolioside A, at their respective retention times. The chemical structures of these compounds (sourced from www.PubChem.com) have been depicted alongside the respective identified peaks. The quantification of these phytometabolites against the standard mix is listed in Table 3, along with the observed retention times.
Fevogrit treatment attenuated fever in LPS‐induced Wistar rats. Antipyretic effect of Fevogrit on LPS‐induced elevation in absolute rectal temperature (A) and change in rectal temperature (B), at different time points over a period of 24 h. The bar graphs (C–E) depict absolute rectal temperature at 4 h post‐LPS administration (C), absolute rectal temperature at 6 h post‐LPS administration (D) and total pyrexia response over the experimental period of 24 h (E) as area under the curve (AUC). For the graphs A and B, data were analyzed by two‐way ANOVA followed by Tukey's multiple comparison test whereas for the bar graphs C, D and E, data were statistically analyzed by one‐way ANOVA followed by Dunnett's multiple comparisons test. All the data are expressed as mean ± SEM (n = 7 or 8 animals per group). ##p < 0.01 vs. NC; *p < 0.05 and **p < 0.01 vs. DC.
Fevogrit modulated circulatory levels of pro‐inflammatory cytokines in LPS‐induced Wistar rats. The bar graphs (A–C) depict the inhibitory effect of Fevogrit treatment on the serum levels of different pro‐inflammatory cytokines, namely, TNF‐α (A), IL‐1β (B) and IL‐6 (C). Data are expressed as mean ± SEM (n = 5–8 animals per group) and were statistically analyzed by one‐way ANOVA followed by Dunnett's multiple comparisons test. ##p < 0.01 vs. NC; *p < 0.05 and **p < 0.01 vs. DC.
Fevogrit regulated the mRNA expression of pro‐inflammatory cytokines in the hypothalamus of LPS‐induced Wistar rats. The bar graphs (A–C) depict the effect of Fevogrit treatment on the mRNA expression of TNF‐α (A), IL‐1β (B) and IL‐6 (C) in the hypothalamus of rats, 24 h post‐LPS administration. Data are expressed as mean ± SEM (n = 5 animals per group) and were statistically analyzed by one‐way ANOVA followed by Dunnett's multiple comparisons test. ##p < 0.01 vs. NC; *p < 0.05 and **p < 0.01 vs. DC.
Fevogrit, a polyherbal medicine, mitigates endotoxin (lipopolysaccharide)‐induced fever in Wistar rats by regulating pro‐inflammatory cytokine levels

July 2024

·

45 Reads

·

1 Citation

Background Fever is characterized by an upregulation of the thermoregulatory set‐point after the body encounters any pathological challenge. It is accompanied by uncomfortable sickness behaviors and may be harmful in patients with other comorbidities. We have explored the impact of an Ayurvedic medicine, Fevogrit, in an endotoxin (lipopolysaccharide)‐induced fever model in Wistar rats. Methods Active phytoconstituents of Fevogrit were identified and quantified using ultra‐high‐performance liquid chromatography (UHPLC) platform. For the in‐vivo study, fever was induced in male Wistar rats by the intraperitoneal administration of lipopolysaccharide (LPS), obtained from Escherichia coli. The animals were allocated to normal control, disease control, Paracetamol treated and Fevogrit treated groups. The rectal temperature of animals was recorded at different time points using a digital thermometer. At the 6‐h time point, levels of TNF‐α, IL‐1β and IL‐6 cytokines were analyzed in serum. Additionally, the mRNA expression of these cytokines was determined in hypothalamus, 24 h post‐LPS administration. Results UHPLC analysis of Fevogrit revealed the presence of picroside I, picroside II, vanillic acid, cinnamic acid, magnoflorine and cordifolioside A, as bioactive constituents with known anti‐inflammatory properties. Fevogrit treatment efficiently reduces the LPS‐induced rise in the rectal temperature of animals. The levels and gene expression of TNF‐α, IL‐1β and IL‐6 in serum and hypothalamus, respectively, was also significantly reduced by Fevogrit treatment. Conclusion The findings of the study demonstrated that Fevogrit can suppress LPS‐induced fever by inhibiting peripheral or central inflammatory signaling pathways and could well be a viable treatment for infection‐induced increase in body temperatures.


Robust anti-tubercular profile of Solanum virginianum extract in enhancing isoniazid bioavailability and curtailing stress tolerance in Mycobacterium smegmatis

July 2024

·

23 Reads

Introduction The formidable survival mechanisms employed by Mycobacterium tuberculosis (Mtb), combined with the low bioavailability of anti-tubercular drugs and their associated hepatotoxicity, worsen tuberculosis management. Traditional medicinal plants offer potential solutions to these challenges. This study focuses on exploring the anti-tubercular potential of Solanum virginianum against Mycobacterium smegmatis, mc²155. Methods and results HPTLC and UHPLC phytochemically characterized the hydro-methanolic extract of Solanum virginianum (SVE). SVE curtails the growth and viability of mc²155 under normal and in vitro stress conditions. The compromised cell wall integrity of mc²155 with SVE is depicted through scanning electron microscopy (SEM) while EtBr permeability assays and TLC-based comparative changes in lipids extraction addressed the integrity of the cell wall. Furthermore, SVE augmented the susceptibility of mc²155 towards Isoniazid (INH) through enhanced bioavailability. Adjunct treatment of SVE with INH demonstrated a markedly reduced survival of the intracellular bacilli. The study also uncovered the hepatoprotective potential of SVE in HepG2 cells. Conclusion This research paves the way for deeper exploration into the potential of Solanum virginianum against virulent Mtb strains, emphasizing over the significance of traditional medicinal plants in tuberculosis treatment. Collectively, the findings suggest SVE as a potent candidate for independent or adjunct anti-tubercular therapy.


Advancements in Nano-Mandoor Bhasma: Unravelling the Particle Size–Ascorbic Acid Synergy for Enhanced Iron Bioavailability for Anemia Treatment

Biological Trace Element Research

Mandoor Bhasma (MB) medicine, based on classical Indian Ayurveda, was size- and surface-modified to improve its therapeutic efficiency for treating iron-deficient anemia. Physical grinding reduced the size of MB to the nanoparticle (nano-MB) range without changing its chemical composition, as measured by particle size distribution. The surface of nano-MB was modified with ascorbic acid (nano-AA-MB) and confirmed using scanning electron microscopy and Fourier transformed infrared spectroscopy. Enhanced iron dissolution from the surface-modified nano-AA-MB under neutral-to-alkaline pH conditions, and in the intestinal region of the simulated gastrointestinal tract (GIT) digestion model was determined using inductively coupled plasma mass spectroscopy. GIT digestae of MB microparticles and nano-AA-MB were found to be biocompatible in human colon epithelial (Caco-2) cells, with the latter showing threefold higher iron uptake. Subsequently, a dose-dependent increase in cellular ferritin protein was observed in the nano-AA-MB digestae-treated Caco-2 cells, indicating the enhanced bioavailability and storage of dissolved iron. Overall, the study showed that reducing the size of centuries-old traditional Mandoor Bhasma medicine to nanoscale, and its surface-modification with ascorbic acid would help in enhancing its therapeutic abilities for treating iron-deficient anemia.


Divya-WeightGo combined with moderate aerobic exercise remediates adiposopathy, insulin resistance, serum biomarkers, and hepatic lipid accumulation in high-fat diet-induced obese mice

May 2023

·

58 Reads

·

9 Citations

Biomedicine & Pharmacotherapy

Obesity has become an unprecedented epidemic worldwide owing to a prolonged imbalance between energy intake and expenditure. Available therapies primarily suppress energy intake but often fail to produce sustained fat loss, necessitating a more efficacious strategy to combat obesity. In this study, a polyherbal formulation, Divya-WeightGo (DWG) has been investigated for its anti-obesity activity using in-vitro and in-vivo assays. Ultra-high performance liquid chromatography (UHPLC) analysis revealed the presence of phytocompounds including gallic acid, methyl gallate, corilagin, ellagic acid, pentagalloyl glucose, withaferin A and hydroxycitric acid, proven to aid in weight loss. The exposure of 3T3-L1 cells to DWG at cytosafe concentrations inhibited lipid and triglyceride accumulation and downregulated the expression of several adipogenic and lipogenic markers like PPARy, C/EBPα, C/EBPβ, SREBP-1c, FASN and DGAT1. DWG reduced LPS-induced pro-inflammatory cytokine release and NF-κB activity in THP-1 cells. The in-vivo anti-obesity activity of DWG, both alone and in combination with moderate aerobic exercise, was assessed in a high fat diet-induced obese mouse model. DWG mitigated the obesity associated increased body weight gain, feed efficiency ratio, glucose intolerance, diminished insulin sensitivity, dyslipidemia, altered liver function profile, lipid accumulation and adiposopathy in obese mice, alone as well as in combination intervention, with better efficacy in the combination approach. Thus, the findings of this study suggest that DWG could be a promising therapeutic avenue to treat obesity through attenuation of lipid and fat accumulation in liver and adipose tissues and could be utilized as an adjunct with lifestyle interventions to combat obesity and associated complications.


Anti-oxidant response of lipidom modulates lipid metabolism in Caenorhabditis elegans and in OxLDL-induced human macrophages by tuning inflammatory mediators

January 2023

·

31 Reads

·

10 Citations

Biomedicine & Pharmacotherapy

Atherosclerosis is the main pathological process of several cardiovascular diseases. It may begin early in life and stay latent and asymptomatic for an extended period before its clinical manifestation. The formation of foamy macrophages due to dysregulated lipid metabolism is a key event in the development and progression of atherosclerotic plaque. The current pharmacotherapy for atherosclerosis is not able to address multiple aetiologies associated with the disease. Lipidom, an herbal prescription medicine, has anti-oxidant, lipid lowering and anti-inflammatory properties that lead to multifaceted treatment benefits against chronic inflammation, dyslipidaemia, and oxidative stress. The present study aimed to characterize the pharmacological effects of Lipidom using various experimental models. The phytochemical analysis of Lipidom was performed on ultra-high performance liquid chromatography (UHPLC) platform. Lipidom was evaluated for cytosafety, IL-1β and MCP-1 release, modulation of NLRP3 pathway, NFκB activity, ROS generation, lipid accumulation and gene expression in THP1 macrophages. Furthermore, Lipidom evaluation was also performed in the N2, CF1553, and TJ356 strains of Caenorhabditis elegans (C. elegans). The evaluation of brood size, adult (%), lipid accumulation, triglyceride levels, SOD-3 GFP signal, MDA formation, DAF-16 nuclear translocation, and gene expression was performed in C. elegans. Lipidom treatment significantly reduced the inflammatory mediators, lipid accumulation, oxidative stress, and normalized genes involved in the development of foamy macrophages. Lipidom treated C. elegans showed a significant decline in lipid accumulation and oxidative stress. Taken together, Lipidom treatment showed a multifaceted approach in the modulation of several mediators responsible for the development and progression of atherosclerotic plaque.


Anti-hyperglycemic contours of Madhugrit are robustly translated in the Caenorhabditis elegans model of lipid accumulation by regulating oxidative stress and inflammatory response

December 2022

·

371 Reads

·

9 Citations

Background The prevalence of diabetes has considerably increased in recent years. In the long run, use of dual therapy of anti-diabetic agents becomes mandatory to attain euglycemia. Also, the incidences of diabetes-related co-morbidities have warranted the search for new therapeutic approaches for the management of the disease. Traditional herbo-mineral, anti-diabetic agents like Madhugrit are often prescribed to mitigate diabetes and related complications. The present study aimed to thoroughly characterize the pharmacological applications of Madhugrit. Methods Phytometabolite characterization of Madhugrit was performed by ultra-high performance liquid chromatography. Evaluation of cell viability, α-amylase inhibition, glucose uptake, inflammation, and wound healing was performed by in vitro model systems using AR42J, L6, THP1, HaCaT cells, and reporter cell lines namely NF-κB, TNF-α, and IL-1β. The formation of advanced glycation end products was determined by cell-free assay. In addition, the therapeutic potential of Madhugrit was also analyzed in the in vivo Caenorhabditis elegans model system. Parameters like brood size, % curling, glucose and triglyceride accumulation, lipid deposition, ROS generation, and lipid peroxidation were determined under hyperglycemic conditions induced by the addition of supraphysiological glucose levels. Results Madhugrit treatment significantly reduced the α-amylase release, enhanced glucose uptake, decreased AGEs formation, reduced differentiation of monocyte to macrophage, lowered the pro-inflammatory cytokine release, and enhanced wound healing in the in vitro hyperglycemic (glucose; 25 mM) conditions. In C. elegans stimulated with 100 mM glucose, Madhugrit (30 µg/ml) treatment normalized brood size, reduced curling behavior, decreased accumulation of glucose, triglycerides, and lowered oxidative stress. Conclusions Madhugrit showed multimodal approaches in combating hyperglycemia and related complications due to the presence of anti-diabetic, anti-inflammatory, anti-oxidant, wound healing, and lipid-lowering phytoconstituents in its arsenal. The study warrants the translational use of Madhugrit as an effective medicine for diabetes and associated co-morbidities.


Citations (13)


... The encouragement of multidisciplinary partnerships between THPs, scientists, and regulators would bring together both traditional knowledge and modern expertise [281]. It would also preserve the valuable wisdom of THPs and indigenous communities while exercising the highest standards of modern care [282]. ...

Reference:

Extraction and Processing of Bioactive Phytoconstituents from Widely Used South African Medicinal Plants for the Preparation of Effective Traditional Herbal Medicine Products: A Narrative Review
Divya-WeightGo combined with moderate aerobic exercise remediates adiposopathy, insulin resistance, serum biomarkers, and hepatic lipid accumulation in high-fat diet-induced obese mice
  • Citing Article
  • May 2023

Biomedicine & Pharmacotherapy

... The mRNA expression analysis was performed as previously described [22]. Briefly, the total RNA was extracted using RNeasy mini kit (Cat# 74104, Qiagen, Germany) and the cDNA synthesis was done using Verso cDNA synthesis kit (Cat# AB1453A, Thermo Scientific, USA). ...

Anti-oxidant response of lipidom modulates lipid metabolism in Caenorhabditis elegans and in OxLDL-induced human macrophages by tuning inflammatory mediators
  • Citing Article
  • January 2023

Biomedicine & Pharmacotherapy

... This effect is supported by other studies which suggest that the components of Ag+ZnO+EU enhance integrin expression and cell adhesion molecules, aiding in efficient cell migration and attachment even under hyperglycemic conditions. This capability is particularly beneficial for diabetic wound healing, where prolonged high glucose levels can otherwise delay the wound closure process [27,92]. ...

Anti-hyperglycemic contours of Madhugrit are robustly translated in the Caenorhabditis elegans model of lipid accumulation by regulating oxidative stress and inflammatory response

... For ferulic acid estimation and quantification individual and with simultaneously HPTLC [15] method is available. For estimation and quantification of rutin individually and simultaneous HPTLC [16][17], RP-HPLC [18], LC/MS [19], UP-HPLC/MS-QToF [20], HPLC [21], UV [22] etc. analytical methods are available. For estimation and quantification of berberine individually and simultaneously HPTLC [23][24], RP-HPLC [25], HPLC [26] etc. analytical methods are available. ...

Identification, Validation and Standardization of Bioactive Molecules Using UPLC/MS-QToF, UHPLC and HPTLC in Divya-Denguenil-Vati: A Penta-Herbal Formulation for Dengue Fever

Chromatographia

... Our findings revealed that glucuronide, a metabolite of piperine, was associated with an increased risk of gout. Piperine is a phytochemical known for its anti-inflammatory properties [23]. Interestingly, previous experimental evidence has shown that piperine can inhibit inflammation induced by sodium urate crystals, suggesting its potential as a therapeutic agent for gouty arthritis prevention and treatment [24,25]. ...

Front Cover: Standardization and validation of phytometabolites by UHPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul
  • Citing Article
  • May 2022

Journal of Separation Science

... The IC50 of the standard was found to be 175 µg/mL, while the test extract had an IC50 of 190 µg/mL, which was nine-fold higher than the value reported previously for this plant [22]. UV absorption λmax of the purified compound was very close to λmax guggulsterol reported from the Commiphora spp. at 257 nm [23]. Mass spectrum analysis of this compound gave an m/z value of 432. ...

Standardization and validation of phyto‐metabolites by Ultra‐HPLC and high‐performance thin layer chromatography for rapid quality assessment of ancient ayurvedic medicine, Mahayograj Guggul
  • Citing Article
  • February 2022

Journal of Separation Science

... Visa vilvadi gulika comprised of Bilva, Tulasi, Karanja, Tagara, Devadaru, Marica, Daruharidra, Ajamutra, Haritaki, Vbhitaki, Amalaki, Sunti, Pippali, Haridra, Pathya, Nilini and Isvari. They offered these kashayam at 50, 50, 50 ml on an empty stomach, after food in the morning and before food and finally concluded that these Ayurvedic medicines boost the immunity of patients with post-COVID-associated mucormycosis (69 (72) implemented Anu taila to cure mucormycosis; Anu taila is comprised of tej patra, vidang, nagkesar, chandan, tavak, bala, yeshtimadhu and daru haldi. The consumption of Anu taila improved the immune response against Mucor spores by activating pre-treated human THP-1 cells and TNF-α. ...

Anu taila , an herbal nasal‐drop, suppresses mucormycosis by regulating host TNF‐α response and fungal ergosterol biosynthesis
  • Citing Article
  • January 2022

Journal of Applied Microbiology

... The extract was passed through a 0.45 µM filter before storing at 4℃ for subsequent experiments. The protocol follows a similar study for extract preparation 17 . B. hispida extract was obtained by boiling B. hispida in water, which was used in the preparation of the confection from the same batch. ...

Chyawanprash, An Ancient Indian Ayurvedic Medicinal Food, Regulates Immune Response in Zebrafish Model of Inflammation by Moderating Inflammatory Biomarkers

... 57 Coronil is also a combination herbal remedy designed as a supporting measure for COVID-19, it contains palmatine as one of several compounds. 58 There are numerous studies indicating the effects of the Berberis vulgaris extract on various organs and systems within the body ( 56 and references therein) these effects might all align to help prevent as well as treat the infection. ...

Phyto‐metabolite profiling of Coronil, a herbal medicine for COVID‐19, its identification by mass‐spectroscopy and quality validation on liquid chromatographic platforms
  • Citing Article
  • September 2021

Journal of Separation Science

... The phenolics compounds have been identified in Fraction 4 are 1-(4-Hydroxy-3-methoxy)-phenyl-1,2,3-propanetriol and rengyolester. Some extracts that are reported to contain rengyoester and have antioxidant properties include non-polar fraction of ethyl acetate extract of Nepthea sp [43] and Forsythiae fructus [44]. Other compounds contained in Fraction 4 are not phenolic compounds but are antioxidants, namely digiprolactone [45] and δ-Humulene [46]. ...

Comprehensive and rapid quality evaluation method for the ayurvedic medicine Divya-Swasari-vati using two analytical techniques: UPLC/QToF MS and HPLC-DAD

Pharmaceuticals