Mayumi Ito’s research while affiliated with The Graduate Center, CUNY and other places

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Publications (49)


The development of hair follicles and nail
  • Literature Review

May 2024

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38 Reads

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1 Citation

Developmental Biology

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Sarah Platt

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Chae Ho Lim

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[...]

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Peggy Myung



HG McSCs possess self-renewal ability
a, Left, bright-field image of a X-gal-stained HF from a DctLacZ mouse at telogen. Right, quantification of McSCs in specified locations in telogen HFs from DctLacZ mice. N = 52 single, whole HFs from 3 mice. b, Timeline of in vivo imaging of single HG McSCs in TyrcreER;RosaLSL-tdTomato;K14rtTA;tetOH2B-GFP mice. HFs containing single HG tdTomato-labelled cells were identified at the first telogen stage and then revisited at indicated stages. c, Live z-stack images of three representative examples of tdTomato⁺ HG McSCs that undergo distinct fates. K14⁺ epithelial cells (GFP⁺) are in green. See Extended Data Fig. 2 for additional examples. Yellow dashed line outlines club hair. White asterisk marks tdTomato⁺ cell in unrelated HF (bottom). d, Quantification of distinct fates of HG McSCs. N = 59 HFs from 7 mice. e, Percentages of tdTomato⁺ bulb MCs that die at the end of anagen or survive during the catagen phase. N = 20 HFs from 2 mice. f, Bar graph showing the furthest distance between two tdTomato⁺ cells in the bulge/ORSup (at late anagen and early/mid catagen) and bulge/HG (at late catagen and second telogen). Data are presented as the mean ± s.d. P values (one-way analysis of variance (ANOVA) with Bonferroni multiple comparison test) are indicated, with 95% confidence interval at 35.81–81.13. N = 21 (late anagen), 27 (early/mid catagen) or 23 (late catagen and telogen) HFs from 2 mice. g, Percentages of tdTomato⁺ McSCs residing in bulge or HG at second telogen. N = 60 HFs from 6 mice. Dashed black or white lines outline the epithelial–dermal boundary (a,c). Scale bars, 20 μm (a) or 10 μm (c). Bb, bulb; Bg, bulge.
Source data
McSCs in the HG initiate a differentiation programme during early anagen
a, z-stack images of tdTomato⁺ McSCs from TyrcreER;RosaLSL-tdTomato mice at telogen and early anagen. Denoted areas are magnified and reconstructed using Imaris (magenta, tdTomato⁺; green, DAPI). b, Left, number of dendrites per tdTomato⁺ McSCs (N = 3 mice, ≥30 tdTomato⁺ McSCs analysed per mouse). Right, percentages of HFs containing only dendritic McSCs (N = 3 mice, 20 HFs analysed per mouse). Melanocytes with ≥3 dendrites were considered dendritic. Data are presented as the mean ± s.d. P values derived by two-tailed unpaired t-test. c, Live revisits of a representative first telogen HF containing a single HG tdTomato-labelled cell in a TyrCreER;RosaLSL-tdTomato;K14rtTA;tetOH2B-GFP mouse. K14⁺ epithelial cells (GFP⁺) are in green. Bottom panels show magnified views. d, Dendrite number per tdTomato⁺ McSCs in HG (telogen and early anagen) and bulge/ORSup (mid-anagen and late anagen). N = 27 HFs from 2 mice. Data are presented as the mean ± s.d. P values derived using one-way ANOVA with Bonferroni multiple comparison test, with the following 95% confidence intervals: −4.804 to −3.122 (telogen versus early anagen); 2.430 to 4.112 (early anagen versus mid-anagen). e, Uniform manifold approximation and projection (UMAP) plot of merged FACS-isolated telogen McSCs, early-anagen McSCs and differentiated anagen VI bulb melanocytes. FeaturePlots show expression of pigmentation genes. f, Percentages of Oca2⁺ cells within each population. g, UMAP plot of early-anagen McSCs after regression of cell cycle genes. The FeaturePlot shows Oca2 expression. h, Violin plots showing differential expression of pigmentation genes in early-anagen Oca2⁺ and Oca2⁻ clusters. i, Schematic of melanocyte differentiation trajectory and McSC composition in the niche. Dashed white lines outline the epithelial–dermal boundary (a,c). Scale bars, 20 μm (a) or 10 μm (c).
Source data
Oca2⁺ cells located in the TA compartment can undergo dedifferentiation during anagen
a–c, Oca2creER;RosaLSL-tdTomato mice were injected with tamoxifen three times during depilation-induced anagen onset for Oca2⁺ cell lineage tracing. a, Detection of tdTomato and Oca2 (fluorescence in situ hybridization (FISH)) as indicated. Denoted areas of tdTomato-only detection are reconstructed using Imaris (magenta, tdTomato⁺; green, DAPI). Magnified views of tdTomato immunofluorescence (IF) and OCA2 FISH are represented in single colour. b, Number of tdTomato⁺ cells over time. N = 5 mice (anagen onset and telogen) and N = 3 mice (2-year telogen). Five areas were analysed per mouse. P values were derived using one-way ANOVA with Bonferroni multiple comparison test, with 95% confidence intervals of −0.7397 to 1.993. c, Detection of tdTomato in telogen and induced anagen at 2 years following tamoxifen treatment. d, Left, timeline of injections and analysis 7 days after depilation of tdTomato expression in Oca2creER;RosaLSL-tdTomato mice treated with PBS (control) or a c-Kit-neutralizing antibody. Right, images and quantification of tdTomato⁺ McSCs. N = 3 mice. Ten areas were analysed per mouse. P value derived by two-tailed unpaired t-test. e, Live lineage tracing of a single tdTomato⁺ cell of Oca2creER:RosaLSL-tdTomato;K14rtTA:tetOH2B-GFP mice reveals three fates. GFP marks K14⁺ epithelial cells. Cartoon illustrates the relative locations of tdTomato⁺ melanocytes and other melanocytes in the HF. White asterisks indicate tdTomato⁺ cells in an unrelated HF. f, Percentages of single Oca2⁺ cells in the lowest HG region (N = 13 HFs from 1 mouse) or the upper HG (N = 13 HFs from 1 mouse) that give rise to specific progeny as defined. Insets in mid/late anagen images in a and c show bright-field images of the bulb. For b and d, data are presented as the mean ± s.d. Scale bars, 20 μm or 10 μm (single colour images of a). Dashed white lines outline the epithelial–dermal boundary (a,c,d,e).
Source data
HF ageing limits the access of McSCs to niche signals that reversibly regulate their differentiation
a, Top, K15crePR1;Wlsfl/fl conditional knockout mice and control mice (7 weeks old) were treated with RU486 and depilated. Bottom left, immunofluorescence of DCT and β-catenin at 3 days after depilation. Bottom right, percentages of DCT⁺ McSCs with nuclear β-catenin. N = 3 mice. b, Top, K15crePR1;Ctnnb1STA mice and K15crePR1;Wlsfl/fl;Ctnnb1STA mice were treated with RU486 from P22. Bottom left, immunofluorescence of DCT, β–catenin and MITF with corresponding bright-field images. Bottom right, percentages of DCT⁺ McSCs with nuclear β-catenin signals and pigmentation. N = 3 mice. c,d, Comparison of DctrtTA;tetOH2B-GFP mice at second and seventh telogen. c, Representative images of mice. Percentages of HFs lacking HG McSC (second telogen: N = 3 mice, 20 HFs analysed per mouse. Seventh telogen: N = 4 mice, ≥36 HFs analysed per mouse). d, Left, z-stack images of HFs with McSCs in GFP. Right, average distance of each McSC from the HG centre (second telogen, N = 3 mice; seventh telogen, N = 4 mice. Five HFs analysed per mouse). Right, percentages of HFs containing bulge McSCs (second telogen, N = 3 mice, 20 HFs analysed per mouse; seventh telogen, N = 4 mice, ≥36 HFs analysed per mouse). e, Live tracing of a single tdTomato⁺ bulge McSC in a TyrcreER;RosaLSL-tdTomato;K14rtTA;tetOH2B-GFP mouse. f, Left, percentages of bulge and HG McSCs that produced differentiated progeny (HG, N =  7 mice, 59 HFs; bulge, N = 6 mice, 8 HFs). Right, the number of divisions by each bulge and HG McSC by mid/late anagen (N = 35 HFs with a single HG McSC; 8 HFs with a single bulge McSC). For a–d,f, data are presented as the mean ± s.d. P values derived by two-tailed unpaired t-test. Dashed white or red lines outline the epithelial–dermal boundary (a,b,d,e). Scale bars, 20 µm (a,b,d), 10 µm (e) or 1 cm (c).
Source data
Dedifferentiation maintains melanocyte stem cells in a dynamic niche
  • Article
  • Full-text available

April 2023

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419 Reads

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60 Citations

Nature

For unknow reasons, the melanocyte stem cell (McSC) system fails earlier than other adult stem cell populations¹, which leads to hair greying in most humans and mice2,3. Current dogma states that McSCs are reserved in an undifferentiated state in the hair follicle niche, physically segregated from differentiated progeny that migrate away following cues of regenerative stimuli4–8. Here we show that most McSCs toggle between transit-amplifying and stem cell states for both self-renewal and generation of mature progeny, a mechanism fundamentally distinct from those of other self-renewing systems. Live imaging and single-cell RNA sequencing revealed that McSCs are mobile, translocating between hair follicle stem cell and transit-amplifying compartments where they reversibly enter distinct differentiation states governed by local microenvironmental cues (for example, WNT). Long-term lineage tracing demonstrated that the McSC system is maintained by reverted McSCs rather than by reserved stem cells inherently exempt from reversible changes. During ageing, there is accumulation of stranded McSCs that do not contribute to the regeneration of melanocyte progeny. These results identify a new model whereby dedifferentiation is integral to homeostatic stem cell maintenance and suggest that modulating McSC mobility may represent a new approach for the prevention of hair greying.

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Solving a molecular cryptogram for the human fingerprint

March 2023

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12 Reads

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2 Citations

Cell

No two fingerprint patterns are exactly alike. In this issue of Cell, Glover et al. uncover the molecular and cellular mechanisms that result in patterned skin ridges over volar digits. This study reveals that the remarkable diversity of fingerprint configurations may originate from a common patterning code.


Wound-Induced Hair Neogenesis Model

October 2022

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50 Reads

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15 Citations

Journal of Investigative Dermatology

Skin wounds in adult mammals typically heal with a fibrotic scar and fail to restore ectodermal appendages, such as hair follicles or adipose tissue. Intriguingly, new hair follicles regenerate in the center of large full-thickness wounds of mice in a process called wound-induced hair neogenesis (WIHN). WIHN is followed by neogenesis of dermal adipose tissue. Both neogenic events reactivate embryonic-like cellular and molecular programs. The WIHN model provides a platform for studying mammalian regeneration, and findings from this model could instruct future regenerative medicine interventions for treating wounds and alopecia. Since Ito et al. rediscovered WIHN 15 years ago, numerous investigators have worked on the WIHN model using varying wounding protocols and model interpretations. Because a variety of factors, including environmental variables and choice of mouse strains, can affect the outcomes of a WIHN study, the purpose of this article is to provide an overview of the experimental variables that impact WIHN so that experiments between laboratories can be compared in a meaningful manner.


Niche stiffness regulates stem cell aging

July 2022

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96 Reads

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3 Citations

Nature Aging

The microenvironment can regulate adult stem cell function during tissue homeostasis and regeneration, but whether and how this is altered in aging is unclear. Ichijo et al. find that increased dermal stiffness, as a result of vasculature atrophy, activates cation channel PIEZO1, leading to interfollicular epidermal stem cell dysregulation.


Tracking skin and immune cell interactions

May 2021

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23 Reads

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2 Citations

Nature Cell Biology

The distribution of skin immune cells, namely Langerhans cells (LCs) and dendritic epidermal T cells (DETCs), is well-documented, but the mechanisms underlying their pattern maintenance remained obscure. A study now finds that LCs maintain their distribution patterns depending upon Rac1 and that the density of LCs and DETCs is regulated by the density of epithelial cells.


Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing

March 2020

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495 Reads

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87 Citations

Science Advances

Human and murine skin wounding commonly results in fibrotic scarring, but the murine wounding model wound-induced hair neogenesis (WIHN) can frequently result in a regenerative repair response. Here, we show in single-cell RNA sequencing comparisons of semi-regenerative and fibrotic WIHN wounds, increased expression of phagocytic/lysosomal genes in macrophages associated with predominance of fibrotic myofibroblasts in fibrotic wounds. Investigation revealed that macrophages in the late wound drive fibrosis by phagocytizing dermal Wnt inhibitor SFRP4 to establish persistent Wnt activity. In accordance, phagocytosis abrogation resulted in transient Wnt activity and a more regenerative healing. Phagocytosis of SFRP4 was integrin-mediated and dependent on the interaction of SFRP4 with the EDA splice variant of fibronectin. In the human skin condition hidradenitis suppurativa, phagocytosis of SFRP4 by macrophages correlated with fibrotic wound repair. These results reveal that macrophages can modulate a key signaling pathway via phagocytosis to alter the skin wound healing fate.



Citations (35)


... This tool helps clarify the molecular mechanisms underlying the transition of hair follicle stem cells from the telogen to anagen phase and explores Krt24's role in this pro-cess. Additionally, this model can be crossed with reporter mice (such as Rosa26 LSL-tdTomato , Rosa26 LSL-DTR ) to perform lineage-tracing of quiescent bulge HFSCs in hair follicle regeneration and wound repair and further reveal their differentiation potential [34,[46][47][48]. Furthermore, this model has the application potential to study how abnormal function of bulge HFSCs leads to hair follicle atrophy or regenerative disorders [35,49], and to study the role of bulge HFSCs in skin tumors [50,51]. ...

Reference:

Generation of the Krt24-Cre Mouse Line Targeting Outer Bulge Hair Follicle Cells
Dedifferentiation maintains melanocyte stem cells in a dynamic niche

Nature

... Clinical application of WIHN has been difficult due to the lack of dermal γδ T cells in human scalps [30], but drug development including Wnt signaling activators is increasing the effectiveness of WIHN in humans [17,43]. Translational studies using various animal models have also made important contributions to the development of potential drugs to induce WIHN in humans [48]. Importantly, the development of these drugs for practical and clinical purposes may provide many opportunities to treat alopecia. ...

Wound-Induced Hair Neogenesis Model
  • Citing Article
  • October 2022

Journal of Investigative Dermatology

... Distribusi sel imun kulit yaitu sel Langerhans (LCs) dan sel T epidermal dendritik (DETCs), terdokumentasi dengan baik, tetapi mekanisme yang mendasari pemeliharaan pola mereka tetap tidak jelas. Sebuah studi sekarang menemukan bahwa LC mempertahankan pola distribusinya tergantung pada Rac1 dan bahwa kepadatan LC dan DETC adalah diatur oleh kepadatan sel epitel 36 . Kulit mewakili organ dalam dan dunia luar. ...

Tracking skin and immune cell interactions
  • Citing Article
  • May 2021

Nature Cell Biology

... The WNT ligand secreted by bulge ESCs activates the proliferation of dermal fibroblasts and formation of DP. However, chronic WNT signaling, stimulated by phagocytosis of the WNT inhibitor SFRP4 by macrophages, is implicated in uncontrolled fibrosis in HS (Gay et al, 2020). DP cells secrete local extracellular vesicles that induce ESC to regenerate the hair follicle through the nonconventional WNT ligand norrin (le Riche et al, 2019), whereas clathrinmediated endocytosis, implicated in the uptake of exosomes, has recently been identified as induced in the tunnel epidermis (Adawi et al, 2025). ...

Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing

Science Advances

... Clusters with fewer than 50 cells were excluded, resulting in a final count of 2776 cells distributed across 10 clusters. As the number of clusters obtained was larger than that obtained in the original study which produced the dataset (Sun et al. 2019), we merged related clusters to create more coarse-grained groupings. The original study performed . ...

A novel mouse model demonstrates that oncogenic melanocyte stem cells engender melanoma resembling human disease

... The WIHN is a regenerative phenomenon that can be exploited in the treatment of alopecia and is regulated by various factors [3,8]. In particular, the WIHN is mainly regulated by the following three factors. ...

Hedgehog stimulates hair follicle neogenesis by creating inductive dermis during murine skin wound healing

... McSCs are critical for pigmented hair-follicle regeneration [26][27][28][29]. McSCs migrate from the hair-follicle niche to the epidermis after wounding and contribute to the generation of unpigmented hairs in the wounds [26]. ...

Dissecting Wnt Signaling for Melanocyte Regulation during Wound Healing
  • Citing Article
  • February 2018

Journal of Investigative Dermatology

... In compliance with the guidelines established by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International, measures were taken to minimize the number of animals used in this study, with the goal of reducing both the number of animals and the associated suffering. In line with the 3Rs principle�replace, reduce, and refine�the number of rats (3 to 5 rats per time point) was determined based on prior studies, 6,24,32,35,36 while three rabbits per test group were deemed adequate for a comprehensive assessment of dermal irritation. 33,37 Female rodents typically show faster wound healing due to the role of 17β-estradiol in enhancing A2A adenosine receptormediated angiogenesis through estrogen receptors and vascular endothelial growth factor (VEGF). ...

Wound Regeneration Deficit in Rats Correlates with Low Morphogenetic Potential and Distinct Transcriptome Profile of Epidermis
  • Citing Article
  • January 2018

Journal of Investigative Dermatology

... Abnormal skin scaring has significantly threatened the health of hundreds of millions of patients, forcing us to develop timely and effective treatment methods [1][2][3]. Keloids, which are tumor-like scars with fibroproliferative and exophytic outgrowing characteristics, can not only cause pruritus and pain but also lead to aesthetic damage and dysfunction [4]. They are easy to occur by abnormally wound healing after injury and even a minor trauma or infection, which however are very difficult to treat and ready to recur due to the unclear etiology and pathogenesis [5,6]. ...

Regeneration of fat cells from myofibroblasts during wound healing
  • Citing Article
  • January 2017

Science

... McSCs are critical for pigmented hair-follicle regeneration [26][27][28][29]. McSCs migrate from the hair-follicle niche to the epidermis after wounding and contribute to the generation of unpigmented hairs in the wounds [26]. ...

EdnrB Governs Regenerative Response of Melanocyte Stem Cells by Crosstalk with Wnt Signaling

Cell Reports