Matthias P. Lutolf’s research while affiliated with Swiss Federal Institute of Technology in Lausanne and other places

... A systematic study is critically needed to confirm this, and this may subsequently allow for the development of appropriate culture conditions and microenvironments which better maintain primary TM cell identity. Since a mechanistic approach was not taken in this study to ascertain why, how and when the divergence in cell identity comparing primary culture to tissues occurs, we point the audience to relatively recent manuscripts/editorials that attempt to draw highlight to this [75][76][77] . With increasing efforts in the use of organoids, microphysiological systems, Springer Nature journal content, brought to you courtesy of Springer Nature Customer Service Center GmbH ("Springer Nature"). ...

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Divergence in cellular markers observed in single-cell transcriptomics datasets between cultured primary trabecular meshwork cells and tissues

... Moreover, in the microsatellite-unstable (MSI) CRC mini-colon where both CAFs and TILs were included, CAFs suppressed T-cell anti-tumor reactivity by promoting the expression of PD-L1. Remarkably, the addition of atezolizumab restored TIL activity also in the presence of CAFs in MSI but not in the microsatellite-stable (MSS) CRC mini-colons [75], clearly demonstrating the superior value of the "mini-colon" device in precision colorectal cancer immunotherapy in a co-clinical setting. ...

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International Journal of Molecular Sciences Review From Cancer to Immune Organoids: Innovative Preclinical Models to Dissect the Crosstalk between Cancer Cells and the Tumor Microenvironment

... There is a growing demand for useful and reproducible in vitro colonic models to predict human drug absorption accurately. Such models are essential, particularly those that yield results comparable to in vivo data (Apostolou et al., 2021;Marr et al., 2023;Mitrofanova et al., 2024;Rozehnal et al., 2012;Sato et al., 2011). ...

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Human organotypic colon in vitro microtissue: unveiling a new window into colonic drug disposition

... 18 These organ-on-a-chip systems have been shown to accurately emulate the physiologically relevant microenvironment of the GIT by incorporating factors such as shear stress, exerted by cyclic strain or fluid flow, and nutrient and oxygen gradients, facilitated by the cellular assembly in a villus structure. [18][19][20][21][22][23] Another notable advantage of organ-on-a-chip platforms is the possibility to integrate complementary sensing strategies, enabling non-invasive, in situ, and real-time monitoring of cellular functions. 24 For monitoring an impaired barrier, an important parameter is barrier integrity, which can be analyzed based on the transepithelial electrical resistance (TEER) using, e.g., traditional chopstick electrodes or impedance sensors. ...

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Sensor-integrated Gut-on-a-Chip for Monitoring Senescence-Mediated Changes in the Intestinal Barrier

... Additionally, this organoid demonstrated typical cholangiocyte receptors: pancreatopoietin and somatostatin. Elci et al. (2024) developed a stable biliary organoid utilizing biliary epithelial cells that displayed essential characteristics of cholangiocytes including transport activity, primary cilium formation, and protective glycocalyx formation over an extended period. Sampaziotis et al. (2019) constructed distinct biliary organoids using human induced pluripotent stem cells (hiPSCs) and primary biliary epithelial cells, which exhibited expression of biliary markers and stable maintenance of biliary cell function. ...

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The utilisation of biliary organoids for biomedical applications

... To ensure utility of ArchMap across datasets from different tissues, Archmap is built to ingest high quality reference atlases integrated using different methods. A wide variety of atlases are available on ArchMap, including atlases accepted by the Human Cell Atlas as o cial tissue references 1,11,[13][14][15][16][17][18][19][20][21] . ...

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ArchMap: A web-based platform for reference-based analysis of single-cell datasets

... Cell source and isolation B16-F10 melanoma cells and pre-activated Pmel-1 TCR transgenic T-cells were received as a kind gift from the Laboratory of Biomaterials for Immunoengineering (EPFL). Colorectal cancer cells, cancer-associated fibroblasts, tumor-infiltrating lymphocytes, and peripheral monocytes were isolated from matched CRC biopsies and peripheral blood obtained from the Centre Hospitalier Universitaire Vaudois (CHUV) as indicated here (Broguiere et al. 16 Table 1, Supplementary Fig. 1a). THP-1 monocytes were purchased from the ATCC (ATCC, Catalog No. TIB-202). ...

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Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

... Most studies on steering human intestinal epithelial cells toward a specific lineage or physiological composition use adult stem cell-derived tissues, either as organoids or in static or microfluidic scaffold-based systems 1,[8][9][10] . These studies have yielded widely used compositions of expansion media and several types of differentiation media, depending on the desired lineage. ...

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An iPSC-derived small intestine-on-chip with self-organizing epithelial, mesenchymal and neural cells

... Studies in mouse and human PSCs have elucidated their susceptibility to aneuploidy, as current methodssuch as culture medium, medium acidification, culture density, and extracellular matrixinduce increased stress on the cells (Bai et al., 2015;Ben-David and Benvenisty, 2012;Keller et al., 2019). This has driven rigorous investigations to understand the underlying mechanisms and led to the formation of guidelines for stem cell research and clinical translation by the International Society for Stem Cell Reseach (ISCCR) (Ludwig et al., 2023). However, to the best of our knowledge, no reports were published on the genomic stability of bovine PSCs in longer-term culture. ...

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Exploring Aneuploidies in Two-Center Isolated Bovine Embryonic Stem Cell Lines: Implications for Cultured Meat Production

... If this receptivity fails, it results in infertility, including repeated pregnancy loss, which is one of the primary reasons for infertility. The receptivity relies on the cross-talk between endometrium and embryo, and various species models were established to mimic the process [22][23][24][25][26][27][28]. Due to the complexity of the process and ethical limitations of using human embryos, establishing an in vitro model is rare despite their necessity [29]. ...

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Establishment of an In Vitro Embryo-Endometrium Model Using Alginate-Embedded Mouse Embryos and Human Embryoid Body