Matthew Berck's research while affiliated with Howard Hughes Medical Institute and other places

Publications (6)

Article
Full-text available
Body temperature homeostasis is essential and reliant upon the integration of outputs from multiple classes of cooling- and warming-responsive cells. The computations that integrate these outputs are not understood. Here, we discover a set of warming cells (WCs) and show that the outputs of these WCs combine with previously described cooling cells...
Article
Odor perception allows animals to distinguish odors, recognize the same odor across concentrations, and determine concentration changes. How the activity patterns of primary olfactory receptor neurons (ORNs), at the individual and population levels, facilitate distinguishing these functions remains poorly understood. Here, we interrogate the comple...
Preprint
Full-text available
Animals can identify an odorant type across a wide range of concentrations, as well as detect changes in concentration for individual odorant type. How olfactory representations are structured to support these functions remains poorly understood. Here, we studied how a full complement of ORNs in the Drosophila larva encodes a broad input space of o...
Preprint
Full-text available
The sense of smell enables animals to react to long-distance cues according to learned and innate valences. Here, we have mapped with electron microscopy the complete wiring diagram of the Drosophila larval antennal lobe, an olfactory neuropil similar to the vertebrate olfactory bulb. We found a canonical circuit with uniglomerular projection neuro...
Article
Full-text available
Significance A previously unidentified set of thermosensory neurons embedded in the olfactory organ of the Drosophila larva is shown to be required to drive the animal up temperature gradients toward preferred environments. Optogenetics and optical neurophysiology reveal efficient sensory encoding of both favorable (warming) and unfavorable (coolin...
Article
Small animals such as nematodes and insects analyze airborne chemical cues to infer the direction of favorable and noxious locations. In these animals, the study of navigational behavior evoked by airborne cues has been limited by the difficulty of precisely controlling stimuli. We present a system that can be used to deliver gaseous stimuli in def...

Citations

... We classified input neurons based on their known sensory modalities. Olfactory (45), gustatory (47,66,101), thermosensory (48,102), visual (46), gut (47,103,104) and respiratory state SNs (105) project directly to the brain. Somatosensory ANs from the nerve cord received direct or indirect input from mechanosensory (29,38,106), nociceptive (38,(107)(108)(109) and proprioceptive SNs (110,111) (Fig. S2, Table S1) and projected to the brain. ...
... These arguments would suggest that the timescales measured in reverse correlation should be similar to the actual timescales of the sensory neuron filters. Indeed, electrophysiological (Schulze et al., 2015;Gorur-Shandilya et al., 2017) and optical (Si et al., 2017) recordings in olfactory sensory neurons reveal that these neurons filter odor inputs with similar dynamics to those observed in our behavioral reverse correlation experiments. ...
... While the number of neurons that were targeted in our tested lines varies from one to seven pairs on average, and sometimes more, in the case when the lines label multiple neuron types, intersectional strategies can be used to further refine the expression patterns. In the larva, a volume of electron microscope data has been acquired and more than 60% of the nervous system has been reconstructed through collaborative efforts [10,21,22,24,25,27,29,[47][48][49]. The synaptic partners of the identified candidate neurons can therefore be further reconstructed in the electron microscopy volume. ...
... Androstadienone is detected at varying concentrations by different individuals, which is similar to many other odors and pheromones; furthermore, it may function as a subliminal odor, or vasana, which operates below the threshold of conscious odor detection [3]. It is known that the activity of olfactory receptor neurons correlates with odorant concentration across a variety of odorants in Drosophila models [16], suggesting a common set of olfactory receptors leading to these differences in androstadienone sensitivity, contributing to its varying role as a pheromone, vasana, or odor. Furthermore, mRNA expression has been shown to change in response to odor concentrations [17], suggesting further that a common set of olfactory receptors for androstadienone may mediate its varying conscious and subconscious effects. ...
... First, changes in facial appearance caused by ocular pathologies can be directly recorded by mobile devices, especially those of the ocular surface or adnexa: for example, eyelid drooping in congenital ptosis. Second and more importantly, individuals may display aberrant behaviors to adapt to changes in their sensory modality, a process conserved from arthropods to mammals 35,36 and confirmed in human children 16 . Our results show that the model can focus on behavioral features replicated in various eye diseases, such as abnormal ocular movement or alignment/fixation patterns. ...
... Animal movement was imaged at 2 fps for 60 min using a Mightex camera (BTE-5050-U). Animal trajectories were detected and analyzed using custom LabView (National Instruments) and MATLAB (Mathworks) scripts (Gershow et al., 2012) (https://github.com/samuellab/MAGATAnalyzer). All assays were performed using one day-old adults. ...