Massimiliano Delpero's research while affiliated with Università degli Studi di Torino and other places
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Publications (60)
The family Galagidae (African galagos or bushbabies) comprises five genera: EuoticusGray, 1872; GalagoGeoffroy Saint-Hilaire, 1796; GalagoidesSmith, 1833; OtolemurCoquerel, 1859; and SciurocheirusGray, 1872, none of which is regarded as monotypic, but some (Euoticus and Otolemur) certainly qualify as oligotypic. We argue for the recognition of a si...
Cytokine gene polymorphisms have been found to be associated with a pre-disposition to a variety of diseases, including inflammatory and cancer diseases. The present study evaluated the influence of six cytokine gene polymorphisms on the level of genomic damage observed in peripheral blood lymphocytes from hospital pathologists chronically exposed...
The increased exposure to environmental pollutants has led to the awareness of the necessity for constant monitoring of human populations, especially those living in urban areas.
This study evaluated the background levels of genomic damage in a sample of healthy subjects living in the urban area of Turin (Italy). The association between DNA damage...
Occupational exposure to anaesthetic gases is one of the major hazards to healthcare personnel. We evaluated the cytogenetic effects of chronic exposure to low concentrations of anaesthetic gases in operating theatres. The study included 21 anesthetists and 21 control subjects who matched in age and gender. Chromosome aberrations (CAs) and sister c...
Alcohol abuse is a significant public health issue. Epidemiological studies conducted on different populations consistently showed that consumption of alcoholic beverages is associated with cytogenetic damages and higher risk for several types of cancer. However, the interpretation of many cytogenetic studies resulted complicated because some confo...
AimSmall body size in Madagascar's dwarf and mouse lemurs (Cheirogaleidae) is generally viewed as primitive. We investigated the evolution of body size in this family and in its sister-taxon, the Lepilemuridae, from phylogenetic, ontogenetic and adaptive perspectives.LocationMadagascar.Methods
We used a phylogenetic method to reconstruct the evolut...
Patterns of genetic variation among geographically and ethnically diverse African populations have been under-represented in human genetics studies despite their importance for describing the evolutionary history of modern human populations. We conducted a study on 133 individuals, belonging to four northern Ivorian ethnic groups, based on the alle...
Chromosome aberrations (CAs) and sister chromatid exchanges (SCEs) frequencies were estimated in peripheral lymphocytes from 21 radiology technicians, and from 21 non-exposed control subjects. We exclusively considered individuals who neither smoke nor consume drugs or alcohol for a period of at least two years prior to the analysis. Significant di...
In the present study, we evaluated the induced genome damage in peripheral blood lymphocytes from a sample of nurses occupationally exposed to low doses of different chemicals. A comprehensive multi-biomarker approach using cytogenetic endpoints was employed for analyzing chromosomal aberrations (CAs) and sister chromatid exchange (SCE) assay. The...
Strepsirhines comprise 10 living or recently extinct families, ≥50% of extant primate families. Their phylogenetic relationships have been intensively studied, but common topologies have only recently emerged; e.g. all recent reconstructions link the Lepilemuridae and Cheirogaleidae. The position of the indriids, however, remains uncertain, and mol...
Cannabinoids, the bioactive constituents of Cannabis sativa, and endocannabinoids, among which the most important are anandamide and 2-arachidonoylglycerol, control various biological processes by binding to specific G protein-coupled receptors, namely CB1 and CB2 cannabinoid receptors. While a vast amount of information on the mammalian endocannab...
Strepsirhines comprise 10 living or recently extinct families, ≥ 50% of extant primate families. Their phylogenetic relationships have been intensively studied, but common topologies have only recently emerged; e.g. all recent reconstructions link the Lepile-muridae and Cheirogaleidae. The position of the indriids, however, remains uncertain, and m...
Diphenylamine (DPA) is an antioxidant compound that occurs naturally in several vegetables. It is widely applied in agriculture for preservation of the quality of apples and pears, and used for controlling superficial scald, a disorder that renders fruits of a number of apple cultivars unfit for the market. Because of its anti-oxidative properties,...
Endogenous nitric oxide (NO) is a molecule synthesized by endothelium nitric oxide synthase encoded by the ecNOS gene that plays an important role in regulating the systemic, cardiac and pulmonary circulation. Impairment in NO synthesis has been associated to many cardiovascular disorders, including coronary artery disease and hypertension. We inve...
Cytokine polymorphisms influence the outcomes of parasitic diseases and vary among populations because of their different evolutionary histories and selective pressures imposed by host-pathogen interactions. In this frame, we investigated the frequencies of TNF-α (-308G/A), TGF-β(1) (codon 10C/T, codon 25C/G) and IL-10 (-1082A/G) SNPs in 133 indivi...
In vitro effects of different concentrations of Thiram were tested on human lymphocytes to determine, by means of the chromosome aberrations (CAs) assay, whether this fungicide could induce clastogenic damage. Evidences of the effect of Thiram on human lymphocytes were limited to sister chromatid exchange, micronuclei formation, and comet assays. W...
To search for cytogenetic signatures that can help to clarify evolutionary affinities among the five orders within the Euarchontoglires
clade, we focused on associations of conserved syntenic blocks that have been accumulated in the karyotypes of Primates (Strepsirhini
and Haplorhini), five families of Rodentia, Scandentia (Tupaia belangeri), Dermo...
The formaldehyde (FA) genotoxic potential in occupationally exposed individuals is conflicting. A relevant indoor-air FA pollution was found in hospitals and scientific institutions where FA is used as a bactericide and tissue preservative. In the present study, we evaluated the frequency of chromosomal aberrations (CAs) in peripheral blood lymphoc...
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Thiabendazole is a benzimidazole-derived compound widely employed in agriculture as anthelmintic and fungicide. It is also used as a post-harvest fungicide for imported citrus fruits during transport and storage, and thus, it was found at high concentration in fruits and vegetables. Several studies have analyzed the potential genotoxic effect of th...
The isonymic method has been generally accepted in population genetic studies and surnames have been successfully used to investigate human populations as if they were genetic markers associated to the Y chromosome. In this study we analysed the microevolution dynamics of Postua, a mountain village of North Western Italy, by means of demographic me...
Eleven human-specific Alu insertion polymorphic loci (ACE, APO, A25, B65, D1, FXIIIB, HS2.36, HS2.43, HS3.23, PV92, TPA25) were analyzed in a population sample of 263 unrelated healthy individuals native of Piedmont (North-Western Italy). Alu insertion frequency and heterozygosity values were highly variable in all loci, except for the HS2.36 locus...
In this study, the frequencies of CYP1A1, GSTM1, and GSTT1 gene polymorphisms were determined in 133 healthy individuals from Ouangolodougou, a small rural town situated in the north of the Ivory Coast. As appeared in several published studies, ethnic differences in these frequencies have been found to play an important role in the metabolism of a...
The COL1A2 gene is one of the two genes encoding for the polypeptides of type I collagen, that represent the major constituent of skin, bone, tendons, ligaments, blood vessels, dentin, and many interstitial tissues. The COL1A2 gene deletion polymorphism has been considered as an informative anthropological marker for describing geographically disti...
Distribution of some erythrocyte polymorphisms was investigated in five Northern Côte d'Ivoire ethnic groups. For the ABO blood group system, the frequencies of alleles p, q and r were 0.119, 0.150 and 0.731, respectively, while the frequencies of alleles D and d of the Rh blood group system were 0.726 and 0.274, respectively. These values are cons...
The present work attempts to determine the distribution of GSTM1 and GSTT1 genotype and allele frequencies in a sample of northern Italian population, and to examine the age-related association of these polymorphisms. The frequencies of the deleted GSTM1 and GSTT1 genotypes were 0.357 and 0.169, respectively. GSTT1 null-genotype frequency found in...
Numerous phylogenetic hypotheses have been advanced for the Malagasy lemuriform radiation, drawing on data from morphology, physiology, behaviour and molecular genetics. Almost all possible relationships have been proposed and most nodes have been contested. We present a phylogenetic analysis, using several analytical methods, of a partial sequence...
Polymorphism frequencies of the dopamine transporter gene (DAT1) hypervariable region have been analyzed in a sample of Italian and Ivory Coast individuals. The 3' untranslated region (UTR) of DAT1 includes a variable number of tandem repeats (VNTR) of a 40-bp monomer, ranging from 3 to 13 repeats in Caucasian and African populations. In our sample...
The ACE and the LRPAP1 gene insertion-deletion polymorphisms were determined in 133 healthy individuals sampled from Ouangolodougou, a village located in northern Ivory Coast. No sex differences were found in ACE and LRPAP1 gene frequencies. The ACE insertion and deletion alleles had frequencies of 0.346 and 0.654, respectively. The ACE gene was no...
We analyzed the frequencies of eight human polymorphic Alu insertion loci in population samples from five towns in North-West Italy: Postua, Cavaglià, Biella, Torino, and Genova. All loci under scrutiny were found to be polymorphic in all samples, with the two exceptions of locus A25 in Postua, which was fixed for the absence of the Alu element, an...
The phylogeny of the Afro-Asian Lorisoidea is controversial. While postcranial data attest strongly to the monophyly of the Lorisidae, most molecular analyses portray them as paraphyletic and group the Galagidae alternately with the Asian or African lorisids. One of the problems that has bedevilled phylogenetic analysis of the group in the past is...
The present work attempts to determine the distribution of ACE and LRPAP1 genotypes and allele frequencies in a sample of the population of north-western Italy and to examine the age-related association of these polymorphisms. ACE D allele frequency found in this work further confirms data obtained in previous studies of Northern Italian population...
Lemuroid phylogeny is a source of lively debate among primatologists. Reconstructions based on morphological, physiological, behavioural and molecular data have yielded a diverse array of tree topologies with few nodes in common. In the last decade, molecular phylogenetic studies have grown in popularity, and a wide range of sequences has been brou...
Aim To examine the phylogeography and population structure of three dung beetle species of the genus Trypocopris (Coleoptera, Geotrupidae). We wanted to test whether genetic differences and genealogies among populations were in accordance with morphologically described subspecies and we aimed to establish times of divergence among subspecies to dep...
With the aim of further investigating the molecular evolution of beta defensin genes, after having analysed beta defensin 1 (DEFB1) in humans and several nonhuman primate species, we have studied the evolution of the beta defensin 2 gene (DEFB2), which codifies for a peptide with antimicrobial and chemoattractant activity, in humans and 16 primate...
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Systematic relationships among the African bush babies are not well understood. Various generic designations are currently in use. Some authors refer all species to a single genus (Galago), while others recognize 4 genera. Phylogenetic reconstructions based on morphology, karyology, allozymes and vocal repertoires have generated inconsistent hypoth...
Citations
... The needle-clawed galagos (Euoticus Gray, 1863) was inferred as sister to the remaining genera, followed by the western dwarf galagos (Galagoides Smith, 1833). In turn, the lesser (Galago É. Geoffroy, 1796) and eastern dwarf galagos (Paragalago Masters et al., 2017) were recovered as sister-taxa. The squirrel galagos (Sciurocheirus Gray, 1872) was inferred as the sister of Otolemur. ...
... The exposure to airborne pollutants can result in harmful health effects, depending on the chemical agents, the time of exposure, and the individual susceptibility [1]. Several studies have focused on hospital workers and their occupational risks [1][2][3][4][5], e.g., operating theatre nurses and pathologists, including those who are chronically exposed to formaldehyde (FA) [6][7][8]. ...
... This value is accepted as a norm in the IAEA recommendations, although according to the data from numerous studies, it can fluctuate within fairly wide limits. Thus, in various articles devoted to the comparison of background levels of chromosome damage detected by different cytogenetic laboratories, the average frequencies of dicentrics (per 1000 cells) varied from 0.30 to 2.99 [28][29][30][31]. ...
... Moreover, it has been demonstrated that the level of the genomic damage also depends on the different individual susceptibility, and single-gene polymorphisms can in uence the susceptibility to MNi formation [16,17]. This susceptibility is in large part due to polymorphisms in metabolic genes, such as cytochrome P450 (CYP) and glutathione-S-transferase (GST) family genes or DNA repair genes as XRCC and XPC genes [17,18,19,20]. ...
Reference: Sportive activity reduces genomic damage
... In fact, they enhance the rate of the DNA strand breaks, leading to chromosomal rearrangements and/or the loss of genetic information, genomic instability, and micronucleus formation [57]. Studies on human peripheral blood lymphocytes show mutagenic activity in alcoholics, with an increased sister chromatid exchange frequency, indicating alterations in the DNA replication or repair processes [58]. Acetaldehyde and other toxic aldehydes produced by lipid peroxidation, such as MDA and 4-hydroxynonenal (4-HNE), damage DNA, as is described in both animal and human studies [59,60]. ...
... Among lemurs, selection for a short life history led to a Table 1. Angiosperm genera comprising species producing seeds dispersed by birds and lemurs (Atsalis, 2008;Birkinshaw, 2001;Bollen et al., 2004), and possible dates of first arrival of the families on Madagascar from Africa associated with three proposed continuous (Palaeocene and Oligocene) and discontinuous (Miocene) land connections [from Masters et al. (2021) reduction of body size in the Cheirogaleidae (Masters et al., 2014(Masters et al., , 2021. Phyletic dwarfing was associated with a dietary shift towards exudativory, i.e. the consumption of gum and nectar (Génin et al., 2010;Andrews et al., 2016), leading to unique plant-animal associations, possibly including other newcomers like the Burseraceae, and with some benefit to the trees [e.g. the cleaning hypothesis of Andrews et al. (2016)]. ...
... The only one proposal that is maintainable, of all this epoch, is the identification of speciesgroup conjugating systematics and homologous chromosomal features [2,14,15]. The fission-fusion theory has been early marginalised by the potentiality of molecular chromosome painting that is now facing a discussion in consideration of the rate of homoplasy that characterizes chromosomal dynamics [16]. Nevertheless, recent cytogenetic results deny a separation of the tribe in two subgroups [17]. ...
... Cytogenetic biomarkers as Sister-Chromatid Exchanges (SCEs), assessed in cultured peripheral blood lymphocytes (PBL), are reliable tools widely employed in human biomonitoring to assess FA-related DNA damage level and, in turn, genotoxic effects (Norppa 2004;Santovito et al. 2014Santovito et al. , 2015Costa et al. 2019). SCEs are the result of DNA replication product interchanges between sister chromatids at apparently homologous loci (Norppa 2004). ...
... asthma, allergy; Farhat et al. 2011). In addition, PBMCs participate in the transport of xenobiotics that is why the analysis of the mechanism of interaction of PS-NPs with this cell type is fully justified (Santovito, Cervella, and Delpero 2014). ...
... Call types and use also differ with sex in Eulemur coronatus (Gamba and Giacoma, & Mutschler, 2006). These species are the largest extant lemurs, and their estimated pairwise divergence time ranges between 18 (Federman et al., 2016;Kistler et al., 2015;Masters et al., 2013) and 29-36 MYA (Antonelli et al., 2017;Fabre et al., 2009;Fritz et al., 2009;Roos et al., 2004). Propithecus diadema lives in multimale/ multifemale groups of two to eight individuals (Irwin, 2008;Powzyk, 1997;Weir, 2014), whereas I. indri groups range from 2 to 5 individuals Glessner & Britt, 2005;Torti et al., 2017;Torti et al., 2018), usually comprising a monogamous reproductive pair and their offspring . ...