Masood Sadiq Butt’s research while affiliated with Sindh Agriculture University and other places

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Publications (274)


Polyphenol-Protein Particles: A Nutraceutical Breakthrough in Nutrition and Food Science
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March 2025

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33 Reads

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1 Citation

Journal of Agriculture and Food Research

Sidra Tul Muntaha

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[...]

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Rana Muhammad Aadil





Flowchart for extraction by a Soxhlet extraction and b supercritical extraction (source [16]
Effect of solvent type and supercritical fluid on extraction yield a Egyptian fennel solvent extract, b Egyptian fennel supercritical fluid extract, c Pakistani fennel solvent extract, d Pakistani fennel supercritical fluid extracts
a % inhibition of standard compounds for ABTS assay, b dose responses for fennel of Egyptian origin, c dose responses for fennel of Pakistani origin
a % inhibition of standard compounds for ABTS assay, b dose responses for fennel of Egyptian origin (c) dose responses for fennel of Pakistani origin
AUC for the calculation of oxygen radical absorbance capacity for Pakistani and Egyptian fennel a fluorescein decay curve, b Trolox standard curve, c AUC for Egyptian fennel, d AUC for Pakistani fennel

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Comparative evaluation of the antioxidant and anti-inflammatory potential of fennel essential oil obtained by conventional and supercritical fluid extraction
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  • Full-text available

February 2024

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283 Reads

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3 Citations

The discovery, progress, and marketing of nutraceuticals and related products are rapidly expanding segments of the food industry which are overtaking the conventional processed food market. The present study investigates the antioxidant, anti-inflammatory, and cellular viability assessment of fennel essential oil. The selection of the extraction process and geographical distribution of fennel play a key role in determining the final composition and hence the corresponding activity of the bio-actives. During the current project, fennel from two origins (Egypt and Pakistan) was procured and subjected to extraction by two methods, i.e., conventional Soxhlet extraction and supercritical fluid extraction, by changing solvent, time, and pressure correspondingly. The resultant extracts were subjected to various analyses, including yield, determination of total phenolic content, and various antioxidant assays. The highest total phenolic content (TPC) was obtained with supercritical fluid extract (206 bar) for Egyptian (180.65 ± 1.22 mg GAE/g) and Pakistani fennel (372.66 ± 0.15 mg GAE/g). The highest values for antioxidant assays were obtained for fennel of Pakistani origin prepared with supercritical fluid extract at 206 bar such as 32.65 ± 0.1 µmole Trolox equivalent/g for FRAP, 74.5% inhibition for ABTS, and 126.25 ± 1.48 µM Trolox equivalent/mL for ORAC. This data obtained suggested that amongst extraction techniques employed, supercritical fluid outstands conventional extraction and essential oil of Pakistani fennel depicted better yield, antioxidant potential, cellular viability, and anti-inflammatory assay than others. The present study paves the way to outline and investigate different factors governing variation in essential oil profiles in response to geographical distribution.

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HPLC chromatograph of hesperidin in (A) methanolic (B) ethanolic and (C) water extract of citrus peel.
HPLC chromatograph of nobiletin in (A) methanolic (B) ethanolic and (C) water extract of citrus peel.
Phytochemical and antioxidant profile of citrus peel extracts in relation to different extraction parameters

January 2024

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665 Reads

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18 Citations

The citrus processing industry generates around 50% fruit waste, encompassing peels, pulp, seeds, and other residues. Citrus peel, among these by-products, contains significant amounts of bioactive compounds, representing a sustainable and renewable source of phenolics. In this context, the present study was conducted to probe phytochemical profile and antioxidant potency of orange peel extracts. Moreover, the quantification of hesperidin and nobiletin was carried out using HPLC. The extracts were obtained using water and 50% aqueous-methanol and aqueous-ethanol, separately alongside varying time intervals, i.e. 30, 45 and 60 min. The obtained extracts were then investigated for their phytochemical and antioxidant profile. Afterwards, three best treatments, one from each extracts (aqueous-ethanol, methanol, and water), were chosen based on their phytochemical profiling. The phytochemical analyses and in vitro antioxidant assays showed the highest total phenolics (2010 mg GAE/100 g), flavonoids (90 mg/100 g), and flavonols (2.2 mg/100 g) in methanolic extracts obtained at 60 min. Likewise, the highest DPPH (60.55%), antioxidant activity (51.7 %), FRAP (13.6 mg TE/g), and ABTS (7.4 µmol TE/g) were also attributed to methanolic extract of orange peel obtained at 60 min except for antioxidant activity which was obtained at 30 min. Likewise, HPLC analysis depicted maximum content of hesperidin and nobiletin (133.70 and 8.50 mg/g) in methanol extract as compared to ethanol (98.80 and 5.50 mg/g) and water (61.90 and 1.25 mg/g), correspondingly. It was concluded that aqueous-methanol could be used as solvent of choice for isolation of orange peel flavonoids with extraction duration of 60 min. Flavonoid and antioxidant levels in orange peel extracts are significantly influenced by extraction time and solvent type.




Fig. 2. The expression level of the insulin signaling pathway (a. INS-1, b. INS-2, c. Pdx-1), calcium signaling pathway (d. Pias-2, e. Calm-2, f. Grk-2), regeneration (g. IGF-1, h. FOXA-1, i. KI67), hormones (j. amylin, k. leptin), and glucose transporter (l. GLUT-2) in the pancreas of the experimental rats Mean values, bearing a different superscript are significantly different from each other (p < 0.05)
Assessment of Antidiabetic and Cyto-Regenerative Activity of Ficus carica through Gene Expression Analysis in Diabetic Rat Model

June 2023

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36 Reads

Proceedings of the Pakistan Academy of Sciences B Life and Environmental Sciences

Diabetes mellitus is a metabolic disease of the endocrine system, characterized by chronic hyperglycemia resulting from insulin resistance or defective insulin production. Among the complementary and alternative medicines, diet-based approaches are gaining popularity worldwide for the management of it. Ficus carica, one of the oldest plants cultivated on the earth, is rich in phytochemicals including anthocyanins, phenolics, flavonoids, and organic acids. The present study was designed to analyze the therapeutic potential of dried fig and extract for their potential against hyperglycemia and related complication in the diabetic rat model. Diabetes was induced by using alloxan monohydrate and divided into five groups including Negative-, Positive-, standard drug- group, treated-I (given extract), and treated-II (given 10% dried figs). Fig extract was administered through the intragastric tube, and fig paste was mixed in the feed of the experimental group, and then rats were decapitated after 6 weeks to collect the blood and serum. At the end of the study, biochemical analysis such as fasting blood glucose (FBG), serum glucose, and insulin was performed. Histopathological study of the pancreas showed cell deformation in the positive control group whereas damage was reversed in treated groups. The pancreas was also saved for gene expression analysis. The results revealed that the positive control group has lower expression of INS-1, INS-2, Pdx-1, amylin, and GLUT-2 genes. Results revealed that serum glucose and FBG started to normalize after the administration of treatment (Glibenclamide, dry fig, and fig fruit extract), and insulin concentration also started to improve. 10% dried fig was more effective to control hyperglycemic conditions, which might be due to the presence of fiber. However, the gene expression was more modulated in the group treated with fig extract. The findings of current research suggested the utilization of fig and fig-based products because of their potential to reverse the damage induced by the alloxan or stressors of daily life.


Citations (76)


... Conversely, circular dichroism and Fourier transform infrared (FTIR) spectroscopy reveal structural changes but not kinetic parameters like binding constants. 13 Molecular dynamics (MD) simulation revealed that the negatively charged form of C3G exhibited high binding probabilities at site I of BSA, while no bindings were observed at site II. 14 Although significant progress has been made in understanding the interactions between ACNs and BSA, several critical issues still remain: (1) most studies on the interaction between ACNs and BSA rely too much on traditional characterization methods; (2) the interaction sites between ACNs and BSA are still unclear and controversial; and (3) the use of molecular docking technology to study the interaction between ACNs and BSA did not take into account the solvation effect and adaptive binding, which lacks rationality. ...

Reference:

Synthesis and characterization of anthocyanin‐loaded bovine serum albumin nanoparticles: unveiling the encapsulation mechanisms with computational insights
Polyphenol-Protein Particles: A Nutraceutical Breakthrough in Nutrition and Food Science
  • Citing Article
  • March 2025

Journal of Agriculture and Food Research

... Rice husk is a byproduct of the rice production process that is often disposed of through open burning, the practice that presents a signi cant challenge to the ecosystem, particularly in developing countries [27][28][29] . Green PLA composites manufactured using rice husk as a reinforcing material offer a novel technology that not only promotes environmental stewardship by reducing waste and carbon footprint, but also enhances prosperity by providing innovative economic opportunities for rice cultivation [30,31] . ...

Valorization of agricultural residues in the development of biodegradable active packaging films
  • Citing Article
  • September 2024

Industrial Crops and Products

... Hemp is a versatile plant with a wide range of applications. It is used to produce paper, textiles, clothing, footwear, building materials, cosmetics, food, pharmaceuticals, home furnishings, disposable crockery, animal bedding, bioplastics and more [50][51][52][53]. Some car manufacturers have started to use hemp for interior components such as dashboards and door panels. ...

Tapping the Nutraceutical Potential of Industrial Hemp against Arthritis and Diabetes - A Comprehensive Review
  • Citing Article
  • April 2024

Food Bioscience

... Figl is a Brassicaceae family and a well-known root vegetable throughout the world. Figl plants are widely grown for their edible roots and leaves in tropical, subtropical, and temperate regions [3]. While the roots are commonly consumed as a vegetable, the leaves and petioles are often underutilized. ...

Journal of Population Therapeutics & Clinical Pharmacology PHYTOCHEMICAL PROFILING AND NUTRITIONAL CHARACTERIZATION OF RAPHANUS SATIVUS ROOTS
  • Citing Article
  • January 2023

... Additionally, the microcapsules showed higher resistance to heat at 65 and 75 • C, with increases of 4 and 2 log cycles, respectively, compared to probiotics that were not encapsulated. In the study of Khan et al. (2024), the viability of Bifidobacterium infantis ATCC 15,697 cells decreased and became non-viable after 28 days of storage. However, the microencapsulated cells maintained a high viability with >106-7 log CFU/g viable cells. ...

Protein-polysaccharide based double network microbeads improves stability of Bifidobacterium infantis ATCC 15697 in a gastro-Intestinal Tract Model (TIM-1)
  • Citing Article
  • January 2024

International Journal of Pharmaceutics

... High-performance liquid chromatographic (HPLC) analysis of orange peel showed that the methanolic extract exhibited highest quantities of nobiletin and hesperidin, measuring 8.50 and 133.70 mg/g, respectively. Comparing the concentrations of nobiletin and hesperidin in the ethanolic extract were 5.50 and 98.80 mg/g, whereas in the aqueous extract, the concentrations were 1.25 and 61.90 mg/g, respectively [12]. A combination of aqueous-methanol solvent systems was preferred for the extraction of flavonoids from orange peels. ...

Phytochemical and antioxidant profile of citrus peel extracts in relation to different extraction parameters

... The significance of harnessing these waste materials has become increasingly prominent in contemporary times, as it serves to safeguard the environment and mitigate the depletion of important nutrients (Gupta et al., 2019;Kringel et al., 2020). For this purpose, various agricultural wastes such as white cabbage (Gül et al., 2013), apple pulp (Negi et al., 2021), soy and citrus industry waste (Sethi et al., 2022), grape pomace and grape seed (Acun & Gül, 2014), cauliflower by-products (Tukassar et al., 2023), sugar beet and carrot (Bajraktari et al., 2024) can be used in food production. The utilization of food items as functional ingredients shows promising potential in relation to both environmental health and functional nutrition (Mohan & Shanmugam, 2016). ...

Compositional profiling and sensory analysis of cauliflower by-products (CBP)-enriched muffins

... The alcoholic extracts of Prunus armeniaca fruits exhibited a noticed anti-proliferative potentials against different cancer cells, colon (HCT-116), human breast (MCF-7), and hepatocellular (Hep-G2) carcinoma cells in a doserelated condition, which are its phytoconstituents, are thought to be responsible for such bioactivities [29]. Moreover, in vivo, the experimental trial showed significant anti-proliferative and chemopreventive potentials of wild prunus extracts in DMBAmediated hepatocarcinomic rats, which are its phytochemicals (anthocyanins, gallic acid, quercetin, syringic acid, ferulic acid, and coumaric acids), are believed to have modulatory effects on serum transaminases, tumor indicators, lowering oxidative stress damage by antioxidant as well as increasing the mRNA expression of CHEK genes and suppressing the ATM signaling cascade [30]. The above-mentioned data validate P. microcarpa extracts as a valuable natural source of anti-cancer agents, suggesting further molecular isolation and investigations on the mechanisms underline its anti-tumor potentials. ...

Phytochemical screening of Prunus avium for its antioxidative and anti-mutagenic potential against DMBA-induced hepatocarcinogenesis

... Yu et al. (2023) found that using thyme essential oil microcapsules (TEOMs) created by the emulsion method and subsequent freeze drying improved the sensory characteristics of mutton patties. Shamshad et al. (2023) conducted a study that demonstrated that incorporating black carrot anthocyanin into ice cream using microencapsulation improved the stability of the anthocyanin and positively affected the sensory attributes of the ice cream. Furthermore, the diameter of microcapsules plays a vital role in the sensory acceptability of food products. ...

Effect of storage on physicochemical attributes of ice cream enriched with microencapsulated anthocyanins from black carrot

... Therefore, it has been proposed that CPC acts as a prodrug activated in the gastrointestinal tract and metabolized into phycocyanobilin (PCB) [25]. In addition, PCB is the biological molecule responsible for other nutraceutical properties such as nephroprotective and anti-inflammatory [17,25]. However, CPC's complete pharmacological action as an asthma treatment is still unknown. ...

Therapeutic efficacy of spirulina against ovalbumin and cigarette smoke‐induced asthma‐specific stress biomarkers in Sprague–Dawley rats