Masako Nishikawa's research while affiliated with The University of Tokyo and other places

Publications (31)

Article
Full-text available
A characteristic clinical feature of COVID-19 is the frequent incidence of microvascular thrombosis. In fact, COVID-19 autopsy reports have shown widespread thrombotic microangiopathy characterized by extensive diffuse microthrombi within peripheral capillaries and arterioles in lungs, hearts, and other organs, resulting in multiorgan failure. Howe...
Article
Vascular stenosis is a pathological hallmark of atherosclerosis, the leading cause of cardiovascular diseases such as stroke and myocardial infarction. While stenosis-induced thrombus formation has been extensively studied using in vivo and in vitro vascular models, the transient and dynamic process of platelet aggregation remains unclear due to th...
Preprint
A characteristic clinical feature of COVID-19 is the frequent occurrence of thrombotic events. Furthermore, many cases of multiorgan failure are thrombotic in nature. Since the outbreak of COVID-19, D-dimer testing has been used extensively to evaluate COVID-19-associated thrombosis, but does not provide a complete view of the disease because it pr...
Preprint
Full-text available
A characteristic clinical feature of COVID-19 is the frequent occurrence of thrombotic events. Furthermore, many cases of multiorgan failure are thrombotic in nature. Since the outbreak of COVID-19, D-dimer testing has been used extensively to evaluate COVID-19-associated thrombosis, but does not provide a complete view of the disease because it pr...
Article
Purpose To establish diagnostic criteria for vitreoretinal lymphoma (VRL) using cytology and laboratory tests from vitreous samples: interleukin (IL)-10/IL-6 ratio, immunoglobulin (Ig) H gene rearrangement, and clonal B-cells on flow cytometry. Methods Fifty-six patients with and 39 without VRL were included. We assessed the sensitivity and specif...
Article
Objectives: Although a single nucleotide polymorphism in a specific receptor for lysophosphatidylserine, a lysophospholipid mediator involved in the immune system, is reportedly associated with Graves' disease, the association between lysophosphatidylserine and thyroid disorders remains to be elucidated. Therefore, we aimed to investigate the asso...
Article
Full-text available
Aims/introduction: Sphingolipids, such as ceramides and sphingosine, are involved in the pathogenesis of diabetes; however, the modulation of urinary sphingolipids in diabetic nephropathy has not been fully elucidated. Therefore, we aimed to develop a simultaneous measurement system for urinary sphingolipids using liquid chromatography-tandem mass...
Article
Full-text available
Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunol-ogy, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural , and morphological phenot...
Article
Full-text available
Lysophospholipids (LPLs) are known to have potentially important roles in the initiation and maintenance of neuropathic pain in animal models. This study investigated the association between the clinical severity of lumbar spinal stenosis (LSS) and the cerebrospinal fluid (CSF) levels of LPLs, using human samples. We prospectively identified twenty...
Article
A quantification system for lysophospholipids (lysoPL) was developed, especially for blood samples, using liquid chromatography–tandem mass spectrometry (LC–MS/MS). However, the lysoPL measurement in cerebrospinal fluid (CSF) has not been validated. Therefore, the present study aimed to validate the lysoPL measurement using CSF samples and to eluci...
Article
Full-text available
GM3 (monosialodihexosylganglioside) is a type of ganglioside, which is a molecule composed of ceramide and oligosaccharide containing one or more sialic acids. Since GM3 is abundantly expressed in blood cells, we investigated the association between GM3 molecular species and haematological diseases. We measured the serum levels of seven GM3 molecul...
Article
Graves’ Disease is a representative autoimmune thyroid disease that presents with hyperthyroidism. Emerging evidence has shown the involvement of lysophosphatidic acid (LPA) and its producing enzyme, autotaxin (ATX), in the pathogenesis of various diseases; among them, the involvement of the ATX/LPA axis in some immunological disturbances has been...
Article
Because the association between sphingosine 1-phosphate (S1P)/apolipoprotein M (ApoM) and chronic kidney diseases has not been established, we investigated the involvement of S1P/ApoM in the phenotypes of IgA nephropathy in hyper-IgA (HIGA) mice. The overexpression of ApoM in adenoviral gene transfer ameliorated the phenotypes of IgA nephropathy in...
Article
Background: Invasion of the central nervous system by haematological malignancies is diagnosed by cytological analyses of cerebrospinal fluid or diagnostic imaging, while quantitative biomarkers for central nervous system invasion are not available and needed to be developed. Methods: In this study, we measured the concentrations of autotaxin an...
Article
Full-text available
The underlying mechanisms of neuropathic pain remain to be elucidated. Basic animal research has suggested that lysophosphatidic acids, which are bioactive lipids produced by autotaxin from lysophosphatidylcholine, may play key roles in the initiation and maintenance of neuropathic pain. Here, we investigated the clinical relevance of lysophosphati...
Article
Full-text available
Dihydro-sphingosine 1-phosphate (DH-S1P) is an analog of sphingosine 1-phosphate (S1P), which is a potent lysophospholipid mediator. DH-S1P has been proposed to exert physiological properties similar to S1P. Although S1P is known to be carried on HDL via apolipoprotein M (apoM), the association between DH-S1P and HDL/apoM has not been fully elucida...
Data
Modulation of SK activity by the overexpression of Band3. Band3 plasmid or GFP plasmid was transfected into K562 cells; 48 hours later, the SK activity was measured as described in the Materials and Methods section. (TIF)
Article
Full-text available
Sphingosine 1-phosphate (S1P) is a bioactive lipid mediator that is thought to be involved in various diseases. Although the main source of S1P in the plasma is erythrocytes, how S1P is exported from erythrocytes has not been elucidated. When we differentiated K562 cells into erythroblast-like cells with sodium butyrate, we observed that the efflux...
Data
Modulation of the expression of enzymes involved in S1P dynamism by the overexpression or knockdown of Band3. Band3 plasmid or GFP plasmid (A) or siRNA against Band3 or control siRNA (B) was transfected into K562 cells; 48 hours later, the expressions of the key enzymes involved in S1P dynamism were determined using real-time PCR. GAPDH was utilize...
Data
Scheme for S1P synthesis and degradation in cells. S1P, sphingosine 1-phosphate; SK, sphingosine kinase; Sgpl, S1P lyase; Spp, S1P phosphatase. (TIF)
Article
Full-text available
Aim: Sphingosine 1-phosphate (S1P) has been suggested to be a positive regulator of plasminogen activator inhibitor 1 (PAI-1) in adipocytes, while some studies are not consistent with this prothrombotic property of S1P. Since S1P is bound to apolipoprotein M (apoM) on HDL or to albumin in plasma, we compared the properties of these two forms on th...
Article
Full-text available
Lysophosphatidic acid (LysoPA) has been proposed to be involved in the pathogenesis of various cancers. Moreover, glycero-lysophospholipids other than LysoPA are now emerging as novel lipid mediators. Therefore, we aimed to elucidate the possible involvement of glycero-lysophospholipids in the pathogenesis of gastric cancer by measuring glycero-LPL...
Article
Full-text available
Lysophosphatidic acids (LysoPA) and lysophosphatidylserine (LysoPS) are emerging lipid mediators proposed to be involved in the pathogenesis of acute coronary syndrome (ACS). In this study, we attempted to elucidate how LysoPA and LysoPS become elevated in ACS using human blood samples collected simultaneously from culprit coronary arteries and per...
Article
Full-text available
Backgrounds: Glycero-lysophospholipids (glycero-LPLs), which are known to exert potent biological activities, have been demonstrated to be secreted from activated platelets in vitro; however, their association with platelet activation in vivo has not been yet elucidated. In this study, we investigated the correlations between the blood levels of e...
Article
Aim: Lysophospholipids, particularly sphingosine 1-phosphate and lysophosphatidic acid, are known to be involved in the pathogenesis of atherosclerosis; however, the role of lysophosphatidylserine (LysoPS) in the onset of atherosclerotic diseases remains uncertain. Methods: We investigated the effects of LysoPS on the uptake of oxidized low-dens...

Citations

... The mean obstruction index (CTOI) was 13.3 ± 14.7%. The CTOI was low (<20%) in 43/61 (70.5%) patients, intermediate (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37).5%) in 13/61 (21.3%), and high (≥40%) in 5/61 (8.2%). Four patients (6.6%) had fatal outcomes with a CTOI of 10, 20, 50, and 52.5%, respectively. ...
... 3 Other authors have assessed cytology, AIGHR, IL-10/IL-6 ratio, and clonal B-cells on flow cytometry and concluded that malignant cytology or positive results for two or more of four tests may be adequate for VRL diagnosis. 4 In our case, AIGHR and cytology were positive and met this criterion. It may be possible to supplement the detection sensitivity by collecting multiple biopsy materials, such as vitreous humor and subretinal fluid, and performing multiple tests. ...
... Unlike the ATX/LPA axis, the biological roles of PS-PLA1/LysoPS in the pathogenesis of those diseases have not been well studied. Recent studies suggest that the PS-PLA1/LysoPS axis is partially involved in the pathogenesis of cancer, acute coronary syndrome, and hyperthyroidism [22][23][24][25] . However, although it has been reported that the PS-PLA1/LysoPS axis inhibits mitogen-induced T-cell activation in vitro 26 , the association between the LPLs-producing enzymes/ LPLs signaling and the disease activity of sarcoidosis remains to be elucidated. ...
... Furthermore, AdipoRon-induced enhancement of lysosomal AC activity lowered cellular ceramide levels, which may contribute to the therapeutic effects of AdipoRon [122]. More recently, a clinical study has found that the elevation of urinary ceramide is associated with DN [123]. According to results from other studies, the abnormal sphingolipid metabolism by lysosomal enzymes may contribute to the elevation of urinary ceramide in DN patients [123]. ...
... Fluorescence-based methods are often disturbed by cell autofluorescence. The fluorescent marker could also have adverse influence on the metabolic process of cells [11,12], and the practically applied markers are limited [13]. Although some proposed new approaches such as cytochrome c can be used as an optical or an electrochemical biosensor for ROS detection [5,14], the temporal resolution cannot match the rapid metabolites change in cell metabolism, and the long-term monitoring stability is lacking. ...
... 27 Furthermore, LysoPA, lysophosphatidylcholine (LysoPC, LPC), LysoPS, LysoPI, lysophosphatidylglycerol (LysoPG, LPG), and lysophosphatidylethanolamine (LysoPE, LPE) were also measured, as described previously. 28,29 We monitored 12 acyl chains (14:0, 16:0, 16:1, 18:0, 18:1, 18 :2, 18:3, 20:3, 20:4, 20:5, 22:5, and 22:6) in the present study. We also measured 155 eicosanoids and related mediators, described in Table S1, together with AA, EPA, and DHA, as described previously, 30 in addition to SM and diacylphospholipids, including phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidylserine (PS). ...
... As in this past study, a correlation between the protein levels of ATX and ATX activity in CSF was observed under higher levels of LPC and LPA in the pathological situation such as inflammation. However, the protein levels of ATX did not correlate with ATX activity under normal or low levels of LPC and LPA in CSF (Hayakawa et al., 2019). The levels of LPA and LPC in CSF were normal or relatively low, which may explain the discrepancy between our current study and previous study. ...
... Additionally, in B-cell lymphomas, GM3-enriched GEMs are essential to improve tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) selective binding to its death receptor (DR4) in malignant cells [284]. GM3 has in fact been recently proposed as a useful serum biomarker for discriminating lymphoid neoplasms from healthy plasma [285]. The fact that GM3 has consistently been associated with lymphomagenesis supports the observation that hexosaminidase, the enzyme responsible of metabolizing GM2 into GM3, could be used as a clinical biomarker for several lymphomas [286][287][288]. ...
... While light microscopy studies revealed a membranoproliferative pattern of glomerular injury with extensive subendothelial deposits, resulting in wire loop appearance, electron microscopy studies showed mesangial and subendothelial deposits of IgG and tubuloreticular aggregates [93]. Proteinuria is one of the clinical manifestations of LN [94,95] and podocyte injury was shown to be one of the main causes of proteinuria in LN and correlates with the severity of the disease [96,97]. Nestin, a cytoskeleton protein which is stably expressed in podocytes, is associated with podocyte injury in LN [98], and was found to protect podocytes by regulating the expression and phosphorylation of Nephrin, thereby reducing proteinuria in mice with LN [98]. ...
... A lumbar puncture, or spinal tap, is used to collect CSF from individuals for subsequent analysis. Although the collection of this biofluid is more invasive than the other biofluids discussed in this review, the gold standard for diagnosis of central nervous system (CNS) infiltration due to haematological malignancies remains the cytological analysis of CSF in order to identify neoplastic cells [163]. CNS invasion is a severe complication of haematological malignancies resulting in significant morbidity and mortality. ...