Marie Walters's research while affiliated with IT University of Copenhagen and other places

Publications (11)

Article
Full-text available
Objective Osteoarthritis (OA) is a significant health issue in humans as well as horses. Experimental models of equine carpal OA have been used to investigate OA pathogenesis and potential therapeutic candidates. A 5-scale scoring system (OARSI) for macroscopic pathological cartilage changes already exists, but there is a need for a scoring system...
Article
Full-text available
Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that the small non-coding RNA expression pattern in synovial fluid and plasma would change during progression of experimental osteoarthritis. In this study, we conducted small RNA sequencing t...
Article
Full-text available
Purpose: Osteoarthritis (OA) is a complex, multifactorial degenerative joint disease affecting multiple tissues within the joint. There are currently no effective curative treatments available, and therapy is often limited to stagnating the disease and supplying analgesia. Novel approaches to understanding OA may lead to discovery of new therapeuti...
Preprint
Full-text available
Extracellular vesicles comprise an as yet inadequately investigated intercellular communication pathway in the field of early osteoarthritis. We hypothesised that small non-coding RNA expression pattern in synovial fluid and plasma would change during progression of experimental osteoarthritis. In this study, we used small RNA sequencing to provide...
Article
Full-text available
Articular cartilage thinning is an important hallmark of osteoarthritis (OA), and ultrasonography (US) is a clinically accessible tool potentially suitable for repeated evaluation. The aim of the present prospective methods comparison study was to validate US as a tool for measuring cartilage thickness in the carpus of the horse. Eight Standardbred...
Article
Full-text available
Objective The objectives of this study was to establish a sensitive and reproducible method to map the cartilage and subchondral bone proteomes in quantitative terms, and mine the proteomes for proteins of particular interest in the pathogenesis of osteoarthritis (OA). The horse was used as a model animal. Design Protein was extracted from articul...
Article
The role of resident cells such a synoviocytes and chondrocytes in intra-articular inflammation is well-characterized, however the in vivo gene expression patterns of cells (predominantly leukocytes) in the synovial fluid (SF) of an inflamed joint have never previously been investigated. The aim of this study was to investigate gene expression in S...

Citations

... The results found that three miRNAs (miR-92a, miR-16 and miR-25) were significantly (FDR < 0.1) upregulated in the synovial fluid collected from horses with mild DIPJ OA compared to severe DIPJ OA. A post-traumatic equine carpal OA experimental model study also identified miR-92a in equine synovial fluid and reported miR-92a expression increased in extracellular vesicles derived from synovial fluid in early OA along with a panel of five other miRNAs (miR-23a, miR-25, miR-215, miR-486-5p and miR-451) [26]. Another equine study profiling miRNA in synovial fluid from the metacarpophalangeal joint showed miR-223 was significantly downregulated and miR-23b was significantly upregulated in the early OA group compared to the control group [25]. ...
... We are currently undertaking a number of studies to identify equine EV markers specic for OA including mass spectrometry lipidomic, NMR lipidomic and small non-coding RNA sequencing studies of equine EVs. In our recent paper 48 we described a number of small non-coding RNAs changing in OA in both plasma and synovial uid derived EVs. Unfortunately, we did not have any remaining plasma from the horses used in this study to complement our ndings here and show the EVs interrogated were OA related. ...
... Some of MS's remarkable applications are in the infection medicine proteomics field, where it is employed to characterize the molecular mechanism behind invasive bacterial diseases [4][5][6], modeling host-pathogen interactions [7][8][9][10][11][12][13] and investigate systemic proteome changes [14][15][16][17][18]. The use of the trypsin protease is justified by its efficiency, stability, and specificity to cleavage only at the C-terminal of the basic residues, arginine, and lysine [19]. ...
... As previously described, the end point synovial membrane scores were significantly increased, with increased cellular infiltration, intimal hyperplasia, subintimal oedema, and increased total final histological score in the OA joints vs. control joints (p < 0.05). Additionally, a previous histological evaluation of the third carpal bone cartilage demonstrated a significant increase in chondrocyte necrosis, cluster formation, and focal cell loss scores in OA joints, and a significant increase in final score (p < 0.05) (54). Representative examples of gross photographs of a control carpal joint and a carpal joint following OA induction plus representative histological sections are shown in Supplementary material 1. ...