María Vergara-Barberán’s research while affiliated with University of Valencia and other places

... Compared to SPME, higher enrichment factors are achieved with SBSE [39]. An aptamer-based application was recently presented to detect allergenic milk proteins from the food matrix using SBSE and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MS) [40]. Alternatively, the stir bar can also be placed in the headspace of a liquid or solid sample (headspace sorptive extraction) [41]. ...

Reference:

Food profiling goes green: Sustainable analysis strategies for food authentication

... While other solid supports, such as glass, alumina, and polystyrene (PV) DVB polymers, have been proposed in the literature, they have not demonstrated significant advantages over the previous [12]. Due to their nature, ISs have been primarily applied to biological compounds [133][134][135][136] and pharmaceuticals [137] in a wide range of matrices, including flours [134,135], meats [137], oranges [136], and milk [133]. The separation methods employed have included CE [134,135], LC [136,137], and matrix-assisted laser desorption/ionization (MALDI) [133] coupled with MS detectors, often achieving recoveries between 77 % and 115 %, and LOD values as low as 0.02 μg mL − 1 [133,137]. ...

Reference:

Miniaturized solid-phase extraction techniques in sample preparation applied to food matrices: A review

... Another type of sorbent based on immobilized aptamers and called oligosorbent (OS) [9,10] is now widely considered as a powerful tool for selective sample preparation prior to LC-MS analysis. Aptamers, i.e. single-stranded DNA or RNA (length ranging from 20 to 60 nucleotides) [9] can fold into unique three-dimensional conformations that allow selective and strong interactions with a specific targeted compound with high affinity and specificity (Kd in the range of nM-µM). ...

Reference:

Preparation and characterization of aptamer-based sorbent for the selective extraction of zearalenone and its derivatives from human urine

... This section will focus on the methodologies developed for honey geographical or botanical origin through liquid chromatography. Table 4 summarizes some selected LC methodologies used for the classification and authentication of honey considering either their geographical or botanical origin [26,65,[68][69][70][71][72]. As can be seen, most of the examples aim to classify honey belonging to only one country using a limited number of samples of each type. ...

Reference:

Spectroscopic, Chromatographic, and Mass Spectrometry Methodologies for Honey Geographical Production and Botanical Origin Characterization and Authentication

... Sample loading time: Loading time must be optimized by introducing the standard protein solution (usually flushing at 930 mbar) at different sample loading times (usually between 2.5 and 20 min, i.e., 30-120 L [36] ). If the protein concentration is not enough to saturate the AA sor- Fig. 2. Effect of the loading time at 930 mbar on the peak area of the eluted -syn [24] (A), Con A proteoforms [26] (B), and -LG proteoforms [27] (C) using the optimized elution and separation conditions and introducing 1 μg·mL − 1 of -syn, 1 μg·mL − 1 Con A and 5 μg·mL − 1 -LG standard solutions. Reproduced with permission. ...

Reference:

On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of protein biomarkers in biological fluids and food: A tutorial

... While other solid supports, such as glass, alumina, and polystyrene (PV) DVB polymers, have been proposed in the literature, they have not demonstrated significant advantages over the previous [12]. Due to their nature, ISs have been primarily applied to biological compounds [133][134][135][136] and pharmaceuticals [137] in a wide range of matrices, including flours [134,135], meats [137], oranges [136], and milk [133]. The separation methods employed have included CE [134,135], LC [136,137], and matrix-assisted laser desorption/ionization (MALDI) [133] coupled with MS detectors, often achieving recoveries between 77 % and 115 %, and LOD values as low as 0.02 μg mL − 1 [133,137]. ...

Reference:

Miniaturized solid-phase extraction techniques in sample preparation applied to food matrices: A review

... Even though metal nanoparticles have shown various positive effects on wound healing, their clinical application raises significant concerns and faces regulatory challenges due to their stability [58], cytotoxicity [59], accumulation [60], long-term safety, and lack of standardized testing protocols [60,61]. ...

Reference:

Current State and Advances in Antimicrobial Strategies for Burn Wound Dressings: From Metal-Based Antimicrobials and Natural Bioactive Agents to Future Perspectives

... In the same context, El-Kholy et al. [37] found 10 phenolic compounds (19.20 µg/mL gallic acid, 191.73 µg/mL catechin, 1.74 µg/mL coffeeic acid, 3.71 µg/mL rutin, 3.91 µg/mL quercetin, 0.46 µg/mL cinnamic acid, 0.56 µg/mL coumaric acid, 0.57 µg/mL ferulic acid, 0.54 µg/mL naringenin, and 0.51 µg/mL propyl gallate) in the ethanol (80%) extract of DPP. For the DPP grown in Egypt, Saudi Arabia, Tunisia, and Algeria, Abdallah et al. [41], Abou Zeid et al. [42], Daoud et al. [43], and Benouamane et al. [44] showed somewhat comparable results, respectively. Numerous biological factors, such as variations in genetics and growing techniques, as well as environmental elements, such as soil conditions, maturation stages, salinity levels, temperature, water availability, and light intensity, can be blamed for this variability [6]. ...

Reference:

Alginate Beads with Encapsulated Date Palm Pollen Extract: Development, Characterization and Their Potential Role in Hepato-Protection and Fertility-Stimulating Hormones Improvement in Bisphenol A-Treated Rats

... However, their limited commercial availability remains a significant challenge, restricting their widespread and routine application. Among these materials, reticular porous structures such as metal-organic frameworks (MOFs) and covalent-organic frameworks (COFs) stand out [121]. MOFs, as a broad category of porous crystalline materials, offer high porosity, tunable compositions and structures, and excellent stability, making them efficient platforms for constructing diverse biosensors. ...

Reference:

Paving the Way Towards Green Contaminant Analysis: Strategies and Considerations for Sustainable Analytical Chemistry

... Indeed, peptides, but specially proteins, require high-selective sorbents to achieve the best performance in SPE-MS, as they are more difficult to ionize and prone to undesired matrix effects. Aptamers, which are typically single-stranded oligonucleotides, are currently regarded as excellent candidates to prepare SPE sorbents for targeting proteins and other bioactive compounds with high affinity and selectivity [27][28][29][30][31][32][33][34]. In addition to their very convenient properties as ligands, aptamers are very stable, easily derivatizable, reusable, and, once appropriately selected, they can be reproducibly produced at low cost [27,28]. ...

Reference:

On-line aptamer affinity solid-phase extraction direct mass spectrometry for the rapid analysis of α-synuclein in blood