Maria Teresa Quaranta's research while affiliated with Istituto Superiore di Sanità and other places

Publications (27)

Article
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Background and Aims Intestinal fibrosis is a common complication of inflammatory bowel diseases. Medical treatment of intestinal fibrosis is an unmet therapeutic need. CD147 overexpression can induce myofibroblast differentiation associated with extracellular matrix deposition, favouring the development of fibrosis. To understand whether CD147 may...
Article
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Metabolism in acute myeloid leukemia (AML) cells is dependent primarily on oxidative phosphorylation. However, in order to sustain their high proliferation rate and metabolic demand, leukemic blasts use a number of metabolic strategies, including glycolytic metabolism. Understanding whether monocarboxylate transporters MCT1 and MCT4, which remove t...
Article
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CD147 is a transmembrane glycoprotein with multiple functions in human healthy tissues and diseases, in particular in cancer. Overexpression of CD147 correlates with biological functions that promote tumor progression and confers resistance to chemotherapeutic drugs. In contrast to solid tumors, the role of CD147 has not been extensively studied in...
Data
β-Dystrobrevin cytosolic protein expression is regulated during RA-induced differentiation of NT2/D1 cells in normoxic and hypoxic conditions. (A, B) Lower panels: Western blot analysis of β-DB cytosolic protein expression in untreated (d0) and RA-treated NT2/D1 cells, under normoxia (21% O2; A) and hypoxia (1% O2; B); Upper panels: densitometry an...
Article
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Duchenne Muscular Dystrophy, a genetic disorder that results in a gradual breakdown of muscle, is associated to mild to severe cognitive impairment in about one-third of dystrophic patients. The brain dysfunction is independent of the muscular pathology, occurs early, and is most likely due to defects in the assembly of the Dystrophin-associated Pr...
Article
High expression of the chemokine receptor 4, CXCR4, associated to negative prognosis in acute myeloid leukemia, is related to hypoxia. Because CXCR4 expression is under the posttranscriptional control of microRNA-146a in normal and leukemic monocytic cells, we first investigated the impact of hypoxia on microRNA-146a and CXCR4 expression during mon...
Article
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Background: The transmembrane 9 superfamily protein member 4, TM9SF4, belongs to the TM9SF family of proteins highly conserved through evolution. TM9SF4 homologs, previously identified in many different species, were mainly involved in cellular adhesion, innate immunity and phagocytosis. In human, the function and biological significance of TM9SF4...
Article
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MicroRNA miR-146a and PLZF are reported as major players in the control of hematopoiesis, immune function and cancer. PLZF is described as a miR-146a repressor, whereas CXCR4 and TRAF6 were identified as miR-146a direct targets in different cell types. CXCR4 is a co-receptor of CD4 molecule that facilitates HIV-1 entry into T lymphocytes and myeloi...
Article
In addition to contrast human immunodeficiency virus (HIV) replication, the HIV protease inhibitors (HIV-PI) have reduced tumour incidence or clinical progression in infected patients. In this regard, we have previously shown that, independently of its anti-viral activity, the HIV-PI indinavir (IDV) directly blocks matrix metalloproteinase (MMP)-2...
Article
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CXCR4 is a negative prognostic marker in acute myeloid leukemias (AMLs). Therefore, it is necessary to develop novel ways to inhibit CXCR4 expression in leukemia. AMD3100 is an inhibitor of CXCR4 currently used to mobilize cancer cells. CXCR4 is a target of microRNA (miR)-146a that may represent a new tool to inhibit CXCR4 expression. We then inves...
Article
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alpha and beta dystrobrevins are cytoplasmic components of the dystrophin-associated protein complex that are thought to play a role as scaffold proteins in signal transduction and intracellular transport. In the search of new insights into the functions of beta-dystrobrevin, the isoform restricted to non-muscle tissues, we performed a two-hybrid s...
Article
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The promyelocytic leukemia zinc-finger protein (PLZF) is a transcription factor and c-kit is a receptor tyrosine kinase associated with human disease, particularly in hematopoietic cells. MicroRNAs (miRs) are post-transcriptional regulators of gene expression, and c-kit has been described as a target of miRs-221 and -222 in erythropoiesis. In the p...
Article
MicroRNAs (miRNAs or miRs) regulate diverse normal and abnormal cell functions. We have identified a regulatory pathway in normal megakaryopoiesis, involving the PLZF transcription factor, miR-146a and the SDF-1 receptor CXCR4. In leukaemic cell lines PLZF overexpression downmodulated miR-146a and upregulated CXCR4 protein, whereas PLZF knockdown i...
Article
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The promyelocytic leukemia zinc-finger protein (PLZF) is a transcriptional repressor. To investigate the role of PLZF in the regulation of cytoadhesion molecules involved in the mobilization of hemopoietic cells, we have analysed PLZF and very late antigen 4 (VLA-4) expression in normal and leukemic cells. In hematopoiesis, we found a negative corr...
Article
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We investigated the expression of the PLZF gene in purified human hematopoietic progenitors induced to unilineage erythroid, granulocytic or megakaryocytic differentiation and maturation in serum-free culture. PLZF is expressed in quiescent progenitors: the expression level progressively rises through megakaryocytic development, whereas it graduall...
Article
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The expression of HOXB cluster genes (i.e., B1 through B9) was evaluated in purified IL-2/IL-1 beta-activated NK lymphocytes from normal adult peripheral blood by RNase protection and reverse transcription-PCR. In quiescent NK cells these genes are essentially not expressed. After IL-2/IL-1 beta addition, we observed a coordinate induction wave in...
Article
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Although the key role of human homeobox (HOX) genes in development is well established, their function in adult cells is still under scrutiny. We have analyzed, in normal adult blood cell subpopulations, acute lymphoid leukemia (ALL) cells lines, and primary blasts, the RNA expression of all HOX-2 cluster genes (5'-2.5, 2.4, 2.3, 2.2, 2.1, 2.6, 2.7...
Article
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Although the key role of human homeobox (HOX) genes in development is well established, their function in adult cells is still under scrutiny. We have analyzed, in normal adult blood cell subpopulations, acute lymphoid leukemia (ALL) cells lines, and primary blasts, the RNA expression of all HOX-2 cluster genes (5′-2.5, 2.4, 2.3, 2.2, 2.1, 2.6, 2.7...
Article
Full-text available
Although the key role of human homeobox (HOX) genes in development is well established, their function in adult cells is still under scrutiny. We have analyzed, in normal adult blood cell subpopulations, acute lymphoid leukemia (ALL) cells lines, and primary blasts, the RNA expression of all HOX-2 cluster genes (5′-2.5, 2.4, 2.3, 2.2, 2.1, 2.6, 2.7...
Article
Full-text available
The intracellular iron level exerts a negative feedback on transferrin receptor (TfR) expression in cells requiring iron for their proliferation, in contrast to the positive feedback observed in monocytes-macrophages. It has been suggested recently that modulation of TfR and ferritin synthesis by iron is mediated through a cytoplasmic protein(s) (i...
Article
IL-6 preferentially promotes the DNA synthesis of human peripheral blood CD8+, rather than CD4+, lymphocytes in presence of PHA: this effect is observed in serum-free cultures of greater than 99% purified CD8+ lymphocytes. However, IL-6 is able to stimulate DNA synthesis of CD8+ lymphocytes triggered by a mitogenic anti-CD2 mAb, but not by anti-CD3...
Article
Full-text available
We have investigated the effect of iron on the expression of transferrin receptors (TrfRs) and ferritin chains in cultures of human peripheral blood monocytes maturing to macrophages. Monocyte-macrophage maturation is associated with a gradual rise of Trf-binding capacity in the absence of cell proliferation. At all culture times, treatment with fe...

Citations

... Both enzyme LDHA and transporters MCT1 and MCT4 exist to play an essential role in cancer cells therefore interference of lactate production by targeting LDHA, MCT1 and MCT4 might be explored as an effective strategy for the development of anticancer therapeutics in various cancers [74]. Moreover, MCT1 and MCT4 play a critical role in leukemic cancer cells, thus inhibition of MCT1 or MCT4 inhibits in vitro proliferation of leukemic cells [75]. Taken together targeting lactate metabolism could be a promising avenue in drug development by targeting GLUT, LDH, MCT1, and MCT4 in various cancers and cancer resistance. ...
... Expression of HOXB genes has been investigated in lymphoid cells. A lack of HOXB gene expression in resting T cells, NK cells, B cells, granulocyte and monocytes has been reported by Petrini et al. (Petrini et al., 1992). Care et al. (1994) have reported that except for very low levels of HOXBl expression, HOXB genes are not expressed in quiescent T cells (Care et al., 1994). ...
... AC-73 inhibited hepatocellular carcinoma metastasis by reducing MMP-2 production by blocking the CD147-stimulated MAPK/STAT3 signaling pathway [101]. In addition, CD147 inhibition by AC-73 resulted in a potent growth inhibitory effect in leukemia cells by deactivating the ERK/STAT3 pathway and activating autophagy, as well as increasing the chemosensitivity of leukemia cells to the conventional antileukemia drugs arabinosylcytosine and arsenic trioxide [102]. In addition to the small-molecule compound targeting CD147, the anti-CD147 drug metuximab (Licartin) prevents tumor recurrence after orthotopic liver transplantation or percutaneous radiofrequency ablation in patients with advanced hepatocellular carcinoma [103,104]. ...
... According to these reports, miR-143 is significantly enriched in neural systems 23 . miR-143 not only participates in neuronal differentiation and development 24,25 , it also mediates the schizophrenia-related locomotor hyperactivity through the dopamine 2 receptor (DA2) in vertebrates 23 . DA2 plays a key role in the dopaminergic system and has been reported to mediate animal behavior. ...
... The identified miRNAs were also confirmed as components of the RISC components. Previous reports have indicated that HIF-1 governs the expression of several miRNAs during hypoxia including miR-210, 67 miR-146a, 68 miR-145, 69 miR-382, 70 miR-191, 71 miR-363, 72 miR-421, 73 miR-204, 74 miR-30a, miR-21, 75,76 miR-687, 77 miR-155, 15 and miR-429 13 and miR-19a, 78 whereas to the best to our knowledge this is the first study identifying HIF-1 and HIF-2 specific, and HIF-2 specific miRNAs. ...
... Functionally, TM9SF4 homologs are involved in cell adhesion, phagocytosis, and innate immunity. [12][13][14][15][16] TM9SF4 also can act on vacuolar H þ -adenosine triphosphatase to regulate cytosolic pH, 17 and regulate cell surface trafficking of glycine-rich transmembrane domains. 18,19 We recently showed an important role of TM9SF4 in maintaining ER homeostasis. ...
... Labbaye et al. showed that promyelocytic leukaemia zinc finger (PLZF) could regulate miR-146a, subsequently controlling the expression of CXCR4 in vitro (Labbaye et al., 2008). Activation of resting CD4+ T cells by phytohemagglutinin results in the downregulation of miR-146a (Quaranta et al., 2015). Downregulation of miR-146a results in the overexpression of CXCR4 co-receptor promoting viral entry in CD4+ T cells (Quaranta et al., 2015). ...
... The two indinavir-containing combinations (mitoxantrone-indinavir and cabazitaxel-indinavir), which failed to conduct the networkbased analysis, also were experimentally confirmed to be synergistic combinations. Indinavir is a human immunodeficiency virus protease inhibitor (HIV PIs), which was proved in vitro and in vivo to slow down the proliferation, promote the apoptosis and inhibit the growth of tumor cells (Toschi et al., 2011;Barillari et al., 2014;Maksimovic-Ivanic et al., 2017). The anti-tumor activity of HIV PIs has also reported in many studies on treating tumors like Kaposi's sarcoma, lymph-gland tumor, or Prostate cancer (Toschi et al., 2011;Barillari et al., 2014;Maksimovic-Ivanic et al., 2017). ...
... In the context of G-CSF stimulation, hsa-miR-146a-5p significantly affects the CXCL12/CXCR4 signaling pathway, which is associated with HSC migration [43]. Hsa-miR-146a-5p influences CXCR4 mRNA expression, which results in the disruption of the CXCL12/CXCR4 complex and subsequent release of CD34+ HSC [20,43,66]. Previous findings indicating the regulatory role of hsa-miR-146a-5p in the CXCL12/CXCR4 axis were performed in vitro in different cell lines [20,43] Our study is the first one evaluating the hsa-miR-146a-5p level in sequential patients' PB samples and correlating its expression with CD34+ cells number in peripheral blood. ...
... A crystallographic study of the RAGE/S100A6 complex indicated that two RAGE ectodomains interact with one S100A6 homodimer [36]; therefore, the structural analysis of the HMG20A/S100A6 complex would be necessary to understand the diversity of the S100A6/target interaction. Previously, it was shown that HMG20A is capable of interacting with β-dystrobrevin, a cytoplasmic component of the dystrophin-associated protein complex, resulting in the regulation of chromatin dynamics, possibly playing a role in neuronal differentiation [37]. The interaction of β-dystrobrevin and the C-terminal fragment (residues 233-342) of HMG20A [37], containing the S100A6-binding region (residues 311-342), raises the possibility that S100A6 may regulate the HMG20A/βdystrobrevin interaction. ...