Maria Steinke's research while affiliated with University Hospital Würzburg and other places

Publications (55)

Article
Full-text available
Due to the wide variety of benign and malignant salivary gland tumors, classification and malignant behavior determination based on histomorphological criteria can be difficult and sometimes impossible. Spectroscopical procedures can acquire molecular biological information without destroying the tissue within the measurement processes. Since sever...
Article
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Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino- and influenza viruses at high concentrations. We sought to investigate whether ASA and its metabolite salicylic acid (SA) inhibit SARS-CoV-2 since it might use similar pathways to influenza viruses. The compound-treated cells were infected with SAR...
Preprint
Full-text available
Aspirin, with its active compound acetylsalicylic acid (ASA), shows antiviral activity against rhino- and influenza viruses and at high concentrations. We sought to investigate whether ASA and its metabolite salicylic acid (SA) inhibit SARS-CoV-2 since it might use similar pathways to influenza viruses. The compound-treated cells were infected with...
Article
Full-text available
Background Anthocyanin-containing plant extracts and carotenoids, such as astaxanthin, have been well-known for their antiviral and anti-inflammatory activity, respectively. We hypothesised that a mixture of Ribes nigrum L. (Grossulariaceae) (common name black currant (BC)) and Vaccinium myrtillus L. (Ericaceae) (common name bilberry (BL)) extracts...
Poster
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Background and Aims: Human cell culture models are best suited for studying host-pathogen interaction in vitro. Using a complex tissue model of the human ciliated airway mucosa (hAM), we aimed to investigate its innate immune response to Bordetella pertussis, the causative agent of whooping cough. We focused on its virulence factor adenylate cyclas...
Article
Full-text available
To study the interaction of human pathogens with their host target structures, human tissue models based on primary cells are considered suitable. Complex tissue models of the human airways have been used as infection models for various viral and bacterial pathogens. The Gram-negative bacterium Bordetella pertussis is of relevant clinical interest...
Article
Full-text available
Background The entity assignment of salivary gland tumors (SGT) based on histomorphology can be challenging. Raman spectroscopy has been applied to analyze differences in the molecular composition of tissues. The aim of this study was to evaluate the suitability of RS for entity assignment in SGT. Methods Raman data were collected in deparaffinize...
Article
Background Sepsis is one of the leading causes of mortality in intensive care units, and sedation in the intensive care unit during sepsis is usually performed intravenously. The inhalative anesthetic sevoflurane has been shown to elicit protective effects in various inflammatory studies, but its role in peritonitis-induced sepsis remains elusive....
Article
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To circumvent time-consuming clinical trials, testing whether existing drugs are effective inhibitors of SARS-CoV-2, has led to the discovery of Remdesivir. We decided to follow this path and screened approved medications "off-label" against SARS-CoV-2. Fluoxetine inhibited SARS-CoV-2 at a concentration of 0.8 µg/ml significantly in these screening...
Article
Full-text available
Bordetella pertussis is a highly contagious pathogen which causes whooping cough in humans. A major pathophysiology of infection is the extrusion of ciliated cells and subsequent disruption of the respiratory mucosa. Tracheal cytotoxin (TCT) is the only virulence factor produced by B. pertussis that has been able to recapitulate this pathology in a...
Article
Full-text available
The human pathogen Bordetella pertussis targets the respiratory epithelium and causes whooping cough. Its virulence factor adenylate cyclase toxin (CyaA) plays an important role in the course of infection. Previous studies on the impact of CyaA on human epithelial cells have been carried out using cell lines derived from the airways or the intestin...
Article
For culture models of primary cells of the human nasal mucosa, monocultures with epithelial cells (EC) are used as well as co-cultures with EC and fibroblasts (FB). Well-differentiated models of the respiratory nasal epithelium can be used for ecogenotoxicological assessments, for experiments on host-pathogen interactions or tissue engineering. How...
Article
3D respiratory tissue models have been generated using, for example, human primary airway epithelial cells (hAEC) or respective cell lines. To investigate ciliopathies, such as primary ciliary dyskinesia, the presence of functional kinocilia in vitro is an essential prerequisite. Since access to hAEC of healthy donors is limited, we aimed to identi...
Article
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Gonorrhea is the second most common sexually transmitted disease in the world and is caused by Gram-negative diplococcus Neisseria gonorrhoeae. Since N. gonorrhoeae is a human-specific pathogen, animal infection models are only of limited use. Therefore, a suitable in vitro cell culture model for studying the complete infection including adhesion,...
Article
Iron oxide nanoparticles have been used in preclinical studies to label stem cells for non-invasive tracking and homing. There is continuous search for novel particle candidates that are suitable for clinical applications. Since standard analyses to investigate cell-particle interaction and safety are labor-intensive, an efficient procedure is requ...
Article
Iron oxide nanoparticles have been used in preclinical studies to label stem cells for non-invasive tracking and homing. There is continuous search for novel particle candidates that are suitable for clinical applications. Since standard analyses to investigate cell-particle interaction and safety are labor-intensive, an efficient procedure is requ...
Poster
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Treatment of malignant pleural mesothelioma (MPM) remains challenging as the tumor is diagnosed at a late stage and only inadequately responds to chemotherapy and radiation [3]. Furthermore the incidence of MPM will increase worldwide over the next years [3]. Preclinical testing proves to be difficult due to a lack of appropriate in vitro tumor mod...
Data
Schematic illustration of the different decellularization protocols used in this study. Each protocol utilizes a different perfusion pressure, volume or duration, respectively. Noteworthy, the SDS- and CHAPS-protocols use only the vascular system as the route of application. Protocols employing Triton-SDC and H2O-SDC apply decellularization solutio...
Data
Evaluation of decellularization protocols. (A) The percentage of airspace in the decellularized matrices was compared to native lungs to quantify the structural preservation for different decellularization protocols. While scaffolds generated using Triton-SDC and H2O-SDC exhibited similar values to native tissue, the CHAPS- and SDS scaffolds showed...
Data
Evaluation of extracellular matrix (ECM) components in decellularized lung scaffolds. (A) Acellular lungs generated by SDS- and CHAPS-protocols tend to contain a lower amount of collagen per mg dry tissue than the scaffolds generated with Triton-SDC or H2O-SDC. While these exhibited significantly higher collagen content than native rat lungs (p = 0...
Data
Analyses performed with different tissue pieces of the lung. (PDF)
Data
Antibodies used for immunohistochemical staining and immunofluorescence. (PDF)
Article
Full-text available
Development of predictable in vitro tumor models is a challenging task due to the enormous complexity of tumors in vivo. The closer the resemblance of these models to human tumor characteristics, the more suitable they are for drug-development and –testing. In the present study, we generated a complex 3D lung tumor test system based on acellular ra...
Data
Separated and sectioned rat lung for analysis. Decellularized and native lung tissue was processed as depicted here to allow different analyses with one scaffold. Except for ultrastructural studies, each analysis was performed with tissue pieces of each part of the lung. The specific use of the single tissue pieces is listed in S1 Table. (TIF)
Data
Histologic assessment of extracellular matrix components in the decellularized lung matrices. All scaffolds showed similar and global retention of the basement membrane components collagen IV and fibronectin. In contrast, a reduced intensity for collagen I and elastin was observed in scaffolds generated with the SDS- and CHAPS– protocols. This was...
Article
In vitro test systems gain increasing importance in preclinical studies to increase the predictivity and reduce animal testing. Of special interest herein are barrier tissues that guard into the human body. These barriers are formed by highly specialized tissues such as the skin, the airways, and the intestine. However, to recapitulate these tissue...
Article
Aim: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture. Materials & methods: We investigated cell-particle interaction and the particles' impact on cell viability, growth and proliferation. We analyzed cell labeling mainten...
Article
In the field of regenerative medicine, we focus on repairing damaged tissue using appropriate cells for therapy that have healing capacities, such as human mesenchymal stem cells (hMSCs). Tissue healing using stem cells will only be possible if the cells can be homed to their target. Beside cell homing at the targeted organ, assessing the cell vita...
Article
Full-text available
Different bioengineering techniques have been applied repeatedly for the reconstruction of extensive airway defects in the last few years. While short-term surgical success is evident, there is a lack of long-term results in patients. Here, we report the case of a young male who received a 5 x 2 cm bioartificial airway patch for tracheo-esophageal...
Poster
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Allogene kryokonservierte Sternumtransplantate stellen eine neue Alternative für die Rekonstruktion komplexer Brustwanddefekte beispielsweise nach onkologischen Resektionen dar. Um einen Anhalt für die zu erwartenden biologischen Eigenschaften dieser Gewebetransplantate zu bekommen, haben wir diese mit im Rahmen von Thoraxoperationenen gewonnenen n...
Poster
Full-text available
Trachealstents auf Nitinolbasis sind mit zahlreichen Komplikationen im Patienten assoziiert. Eine Beschichtung der Stentoberflächen mit Collagen I zeigte in vorausgegangenen Untersuchungen in vitro eine höhere Biokompatibilität. Der klinische Nutzen wurde von uns mittels klinisch eingesetzten Atemwegsstents in einem Großtiermodell ermittelt.
Article
Full-text available
Zielsetzung: Allogene cryokonservierte Sternumtransplantate stellen eine neue Alternative fur die Rekonstruktion komplexer Brustwanddefekte nach onkologischen Resektionen dar. Um einen Anhalt fur die zu erwartenden biologischen Eigenschaften dieser Gewebetransplantate zu bekommen, haben wir diese mit im Rahmen von Thoraxoperationen gewonnenen nativ...
Article
Full-text available
Zielsetzung: Trachealstents auf Nitinolbasis sind mit zahlreichen Komplikationen im Patienten assoziiert. Eine Beschichtung der Stentoberflachen mit Collagen I zeigte in vorausgegangenen Untersuchungen in vitro eine hohere Biokompatibilitat. Der klinische Nutzen wurde von uns jetzt mittels klinisch eingesetzten Atemwegsstents in einem Grosstiermode...
Article
Full-text available
To investigate interrelations of human obligate airway pathogens, such as Bordetella pertussis, and their hosts test systems with high in vitro/in vivo correlation are of urgent need. Using a tissue engineering approach, we generated a 3D test system of the airway mucosa with human tracheobronchial epithelial cells (hTEC) and fibroblasts seeded on...
Article
In vitro test systems have to be optimized to reduce preclinical failure rates. Therefore, we have introduced a new innovative system which mimics physiological conditions and enables vascularisation in bioreactors. Thus, we have developed test systems for intestinal uptake studies, for airway infections and for different tumour entities, e. g. lun...
Poster
The complex pathogenesis of Bordetella pertussis eliciting whooping cough is not completely elucidated, yet. These bacteria are strictly human pathogen and adhere to kinocilia of the respiratory epithelium. Thus, appropriate test systems based on human cells are necessary to study bacteria-host interactions. Tissue-engineered human airway models (T...
Article
Biomedicine represents a new scientific field at the interface of human, molecular and cell biology and medicine. Comprising the diverse disciplines of stem cell research, tissue engineering and material sciences, biomedicine gives rise to new approaches in research and therapy for - to date - unmet medical issues. Biomedical research is currently...

Citations

... In Table 2, we document these findings that feature the anti-KHSV efficacy of flavonoids, and their chemical structures are displayed in Figure 10. According to the results obtained by [112][113][114][115][116], flavonoids ...
... The considerable variety of benign and malignant SGTs becomes obvious by looking at the 2017 WHO Classification of Head and Neck Tumors, which differentiates between more than thirty different benign and malignant SGTs [2]. Unambiguous SGT classification and malignant behavior determination based on histomorphology alone can be challenging and, in some cases, impossible [3], which may complicate particularly a reliable intraoperative frozen section diagnosis. ...
... The inhibitory effects of SEV on apoptosis and inflammation have been reported in both in vivo and in vitro experiments. 30 For example, SEV was found to reduce neutrophil apoptosis. 31 In a vulnerable arterial plaque animal model, SEV promoted plaque stabilization and suppressed plaque disruption by enhancing collagen deposition and inhibiting inflammation. ...
... SARS-CoV-2 is an enveloped RNA virus that comes into the cell via pH-dependent endocytosis. The incoming RNA is translated into viral proteins (Zimniak et al., 2021). Antiviral therapies have been developed to inhibit viral polymerase, integrases, and entry. ...
... Regulation of virulence genes expression is then fine-tuned by the RisA response regulator protein [45,46]. Further, B. pertussis sheds muramyl peptide fragments of peptidoglycan known to act as tracheal cytotoxin (TCT) that triggers NOD1 signaling and NO production, provoking damage of ciliated epithelial layers [47][48][49]. TCT shedding can then be reduced by introduction of a functional copy of the ampG gene into B. pertussis chromosome [50]. Finally, the pertactin (Prn), Fimbriae (Fim) and filamentous hemagglutinin (FhaB) adhesion factors were all shown to mediate attachment of B. pertussis to epithelial layers [2]. ...
... The existence of rare cell types in this system has not been reported. Although these cells are closest to primary cultures, IL-6 secretion and mucociliary differentiation are also impaired compared to primary airway epithelium [71]. Furthermore, this line lacks inflammatory characteristics such as the absence of LPS-induced secretion of pro-inflammatory cytokines (e.g., tumor necrosis factor α [TNFα], interleukin [IL]-1β, IL-12) [72]. ...
... The resulting organotypic bronchoepithelium closely resembles the in vivo airway and is frequently used in studies of toxicology, infection, and drug delivery (Pezzulo et al., 2011;Lenz et al., 2014;Singanayagam et al., 2019;Berthold et al., 2022;Mastalerz et al., 2022). The fully differentiated epithelium, obtained 4 weeks after the airlift, contains all major bronchial epithelial cell types and displays physiologically important functional properties like mucus secretion and ciliary beating (Lodes et al., 2020). The model also allows for differentiating phBECs from different disease origins and assessing viral replication and antiviral epithelial responses after infection (Zarcone et al., 2017;Singanayagam et al., 2019). ...
... Hydrogels are usually placed on a scaffold, and cells can be grown within or on top of the hydrogel, with 7-21 days necessary for full differentiation. These models have been used to analyze bacterial growth patterns, as well as targeted aspects of pathogenicity for multiple pathogens, including ZIKA, HSV, Chlamydia spp., Neisseria gonorrhoeae, and HIV (Medina-Colorado et al. 2017, Nogueira et al. 2017, Zhu et al. 2017, Amerson-Brown et al. 2018, Gorwood et al. 2019, Heydarian et al. 2019, Imle et al. 2019. Similarly, self-assembled organoids can be grown either on a scaffold (i.e. ...
... 21 The growing set of methodologies available to study these phenomena at the molecular level has enabled deeper understanding of surface effects. 5,19,20,[22][23][24][25] For example, new NMR tools have enabled more detailed analysis of the particle surface and its interactions with low affinity proteins. 3,[26][27][28] There have also been several observations, discussed below, that cast a new light on previous understanding. ...
... 3D airway tissue models were generated using porcine-derived decellularized small intestinal submucosa (SIS) without serosa representing the physiological ECM [18]. Small pieces of this collagenous scaffold clamped between two metal rings (cell crown) were placed into 12 well plates. ...