Maria Florencia Fangio’s research while affiliated with National University of Mar del Plata and other places

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Publications (18)


From waste to value: protein hydrolysates from byproducts of the Argentine hake (Merluccius hubbsi) processing using endogenous enzymes and Alcalase® 2.4L
  • Article
  • Full-text available

January 2025

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6 Reads

Marine and Fishery Sciences (MAFIS)

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Analia Fernández-Gimenez

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Maria Florencia Fangio

The valorization of fishery byproducts is essential to reduce waste and create high-value products. Waste from Argentine hake (Merluccius hubbsi) could enhance its functional and antioxidant properties through hydrolysis, releasing peptides with bioactive properties. Protein hydrolysates of Argentine hake were produced through autolysis (Aut) and enzymatic hydrolysis using Alcalase® 2.4L at concentrations of 0.24% and 2% (v/v) (Alc-0.24 and Alc-2), respectively, over 150 min. Alkaline peptidase activity, degree of hydrolysis, and antioxidant activity were assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical ABTS·+ scavenging assays. All hydrolysates retained alkaline peptidase activity throughout the process. Alcalase-treated hydrolysates exhibited significantly higher peptidase activity and hydrolysis degree compared to autolysis. At 60 min, Alc-0.24 reached peptidase activity levels similar to Alc-2, and by 30 min, both had comparable degrees of hydrolysis. ABTS·+ scavenging activity increased over time for Alc-0.24, with both Alcalase® 2.4L concentrations outperforming autolysis. No significant differences were found between Alc-0.24 and Alc-2. Although all hydrolysates showed DPPH scavenging activity, no significant differences were detected between treatments or reaction times. These findings highlight the potential for producing value-added protein hydrolysates from Argentine hake waste.

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Pairwise Pearson correlation coefficient values (r) among carrot bioactive compounds and antioxidant capacity for years 2018 and 2019.
Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids

April 2023

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172 Reads

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11 Citations

Plants

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Sofía Carvajal

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[...]

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The present study characterized a genetically and phenotypically diverse collection of 27 purple and two non-purple (one orange and one yellow) carrot accessions for concentration of root anthocyanins, phenolics, and carotenoids, and antioxidant capacity estimated by four different methods (ORAC, DPPH, ABTS, FRAP), in a partially replicated experimental design comprising data from two growing seasons (2018 and 2019). Broad and significant (p < 0.0001) variation was found among the accessions for all the traits. Acylated anthocyanins (AA) predominated over non-acylated anthocyanins (NAA) in all the accessions and years analyzed, with AA accounting for 55.5–100% of the total anthocyanin content (TAC). Anthocyanins acylated with ferulic acid and coumaric acid were the most abundant carrot anthocyanins. In general, black or solid purple carrots had the greatest TAC and total phenolic content (TPC), and the strongest antioxidant capacities, measured by all methods. Antioxidant capacity, estimated by all methods, was significantly, positively, and moderately-to-strongly correlated with the content of all individual anthocyanins pigments, TAC, and TPC, in both years (r = 0.59–0.90, p < 0.0001), but not with the carotenoid pigments lutein and β-carotene; suggesting that anthocyanins and other phenolics, but not carotenoids, are major contributors of the antioxidant capacity in purple carrots. We identified accessions with high concentration of chemically stable AA, with potential value for the production of food dyes, and accessions with relatively high content of bioavailable NAA that can be selected for increased nutraceutical value (e.g., for fresh consumption).


The TEAC value compares the DPPH radical scavenging capacity of the extracts with the synthetic antioxidant Trolox, a
Total phenolic content, total flavonoids content, saponins content, and antioxidant activity of the hydro-alcoholic extracts of the different organs of B. insignis plant
Chemical characterization and antimicrobial activity of Blumenbachia insignis (Loasaceae) native to South America

November 2022

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50 Reads

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1 Citation

Bulletin of the National Research Centre

Background Blumenbachia insignis is a plant native to Brazil, Uruguay, and Argentina. It is used as an ornamental plant for its flowers, but also, in popular medicine, the stems and leaves are used as antirheumatic agents. Detailed studies on the differential chemical composition of their organs were carried out to establish a relationship with their biological activity and ethnomedicinal uses and the compounds present. Results The presence of phenolic compounds, flavonoids, and triterpenoid saponins was detected in the extracts of the different organs of Blumenbachia insignis , those of the flower being the ones that had the highest concentration of these families of compounds, and also the highest antioxidant and antimicrobial activity. Volatile compounds were identified using gas chromatography coupled to mass spectrometry (GC–MS) in all the extracts. Some of them possess recognized antimicrobial and antioxidant activity among others. On the other hand, the roots showed an important presence of monoterpenes, not so common for these organs. Conclusions These characteristics could be useful to prevent various oxidative stress processes and against pathogenic bacteria.


Characterization of liquid protein hydrolysates shrimp industry waste: Analysis of antioxidant and microbiological activity, and shelf life of final product

April 2021

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144 Reads

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15 Citations

Proteases from shrimp wastes were characterized and protein hydrolysates were obtained. Shrimp Protein Hydrolysates (SPH) were produced by autolysis (H0) and added 1% (H1) and 2% v/v (H2) enzyme extract of shrimp. The hydrolysis degree was determined using a colorimetric method, the capability of hydrolysates to scavenge free radicals was measured with DPPH and the antimicrobial activity of the SPH was evaluated by the microdilution test. The degree of protein hydrolysis ranged between 43% (H0) and 71.5% (H2) after 90 min, and it functioned as a source of Lysine, Leucine, Aspartic acid, Glutamic acid, and Glycine. After 10 min of reaction all hydrolysates reached 50% of scavenging effect. In addition, the SPH prepared with food additives showed acceptable microbiological quality and pH during 40 days at room temperature. This study aims at introducing a low cost process which produces SPH with commercial applications in the food industry.




Morphoanatomic and phytochemical study of Blumenbachia insignis Schrad. (Loasaceae), characterization of compound of interest showing biological activity and the connection with their use in popular medicine

September 2019

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46 Reads

Resumen Los tallos y hojas de Blumenbachia insignis se utilizan en medicina popular como remedio antirreumático. Sin embargo, no hay estudios detallados sobre la composición química diferencial de sus órganos y su raíz ha sido muy poco estudiada, tanto en aspectos morfoanatómicos como fitoquímicos. Es nativa de Argentina y presenta forma de vida hemicriptófita. El objetivo de este trabajo fue realizar una caracterización morfoanatómica de raíz, tallo y hojas e identificar cuantitativamente el contenido de compuestos químicos con actividad biológica. Para determinar la presencia de compuestos fitoquímicos se prepararon extractos de flor, fruto, hoja, tallo y raíz. Se realizaron diferentes técnicas para determinar la presencia de compuestos fenólicos, flavonoides totales y saponinas; la composición se determinó por (GC-MS). Se observó un sistema radical alorrizo con una raíz pivot con grandes reservas de almidón en el parénquima cortical y presencia de gotas lipídicas asociadas a la exodermis. Se halló parénquima amiláceo rodeando los haces vasculares tanto de tallos como de pecíolos. Presencia de tricomas glandulares con contenido lipídico en hojas y tallo. Se detectó la presencia diferencial de compuestos fenólicos, flavonoides y saponinas triterpénicas en los distintos extractos; la flor contiene la mayor concentración de estas familias de compuestos, seguida por hojas y raíces. Se identificaron compuestos volátiles en todos los extractos, algunos de reconocida actividad antimicrobiana y antioxidante como el fitol (flores y hojas), y el mirtanal (raíces). La presencia de metabolitos secundarios con importante actividad antimicrobiana y antioxidante la convertiría en una fuente potencial de compuestos de interés medicinal.


Chemical characterization and antimicrobial activity against Paenibacillus larvae of propolis from Buenos Aires province, Argentina

April 2019

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138 Reads

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21 Citations

In this study, we examined the chemical composition and antimicrobial and radical scavenging activities of propolis from Buenos Aires province, Argentina. The polyphenol and flavonoids content of ethanol extracts of propolis (EEP) vary widely, ranging from 189 to 417 mg gallic acid equivalents (GAE)/g and from 46 to 191 mg equivalents of quercetin (QE)/g, respectively. High-performance liquid chromatography (HPLC) analyses indicate the presence of abscisic acid, p-coumaric acid, epicatechin, indoleacetic acid, and quercetin. All samples show antimicrobial activity against Paenibacillus larvae measured by the agar diffusion method and antioxidant activities, measured with both the ABTS and DPPH assays. In addition, EPR studies indicate that the scavenging activity of EEP towards hydroxyl radicals is high and reaches 71%. The antioxidant activity correlates positively with polyphenols and flavonoids content. Besides, the samples were analyzed by Fourier-transform infrared spectroscopy (FTIR) and UV–visible spectrophotometry. Principal component analysis (PCA) and hierarchical conglomerate analysis (HCA) were applied to the spectra data, clustering samples over the spectral ranges of 1700–1100 cm⁻¹ and 410–240 nm. The results suggest a good predictive capability of FTIR and UV–vis spectroscopy for grouping propolis samples from related geographical origins.


Photochemistry and Photophysics of Shinorine Dimethyl Ester

January 2018

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129 Reads

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2 Citations

Photochemistry and Photobiology

The photostability and photophysical properties of the dimethyl ester of the mycosporine-like amino acid shinorine have been experimentally evaluated in aqueous solution and in the presence of direct micelles prepared with a cationic or an anionic detergent, respectively. In comparison with shinorine, the ester molecule increases the photostability, the fluorescence quantum yield and the fluorescence lifetime in water as well as in the micellar solutions. The effects are more pronounced in sodium dodecyl sulfate solutions and suggest that the electrostatic attractions with the micellar interface contribute to limit the movement of the molecules and influence the relative rate of their deactivation channels. However, the predominance of the non-radiative decay is maintained together with the UV photoprotective ability of this atypical mycosporine species. This article is protected by copyright. All rights reserved.


Two choices for the functionalization of silica nanoparticles with gallic acid: characterization of the nanomaterials and their antimicrobial activity against Paenibacillus larvae

November 2016

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324 Reads

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17 Citations

Journal of Nanoparticle Research

Silica nanoparticles attached to gallic acid were synthesized from 7-nm diameter fumed silica particles by different functionalization methods involving the condensation of hydroxyl or carboxyl groups. The particles were characterized by thermal analyses and UV–vis, FTIR, NMR, and EPR spectroscopies. In comparison to free gallic acid, enhanced stability and increased antimicrobial activity against Paenibacillus larvae were found for the functionalized nanoparticles. Thus, both derivatization strategies result in improved properties of the natural polyphenol as antimicrobial agent for the treatment of honeybee pathologies.


Citations (13)


... Carrot is mainly used in salads, soups, and juices, for colorant production in the food industry, and, more in general, for food preparation due to its high nutritional value [12][13][14][15]. The root of carrot is rich in crude fiber (cellulose, hemicellulose, and lignin), minerals (Ca, P, K, Fe, and Mg), appreciable amounts of vitamins (thiamin, riboflavin, niacin, folic acid, and ascorbic acid), amino acids, carbohydrates (sucrose, glucose, xylose, and fructose), phenols, and anthocyanins in the black/purple varieties [15,16]. ...

Reference:

Health-Promoting Effects, Phytochemical Constituents and Molecular Genetic Profile of the Purple Carrot ‘Purple Sun’ (Daucus carota L.)
Characterization of Purple Carrot Germplasm for Antioxidant Capacity and Root Concentration of Anthocyanins, Phenolics, and Carotenoids

Plants

... The potential of H. lupulus as a controlling agent of pests has been tested as well. Natural bioactive substances obtained from hop extracts, mainly essential oils, were used as an alternative and effective control method for fighting against American foulbrood (AFB) and varroosis diseases caused by Paenibacillus larvae, a flagellated gram-positive bacterium, and ectoparasitic mite Varroa destructor, respectively [59,60]. The effects of the secondary metabolites by the plant and their efficiency against pests/pathogens are depicted in Table 5. ...

Valorization of hop leaves for development of eco-friendly bee pesticides

... attached to the cephalothorax can be recovered by enzymatic hydrolysis and used as a food supplement or in the formulation of culture media (Pereira et al., 2022). Chitin can be used as a healing agent or in soil improvement, as well as in the production of chitosan, N-acetyl-glucosamine (GlcNAc), or glucosamine. ...

Characterization of liquid protein hydrolysates shrimp industry waste: Analysis of antioxidant and microbiological activity, and shelf life of final product

... Propolis and its derived products have been found to exhibit biological activity, for example antimicrobial [5][6][7], anitioxidant [8,9], etc. [10][11][12]. ...

Chemical characterization and antimicrobial activity against Paenibacillus larvae of propolis from Buenos Aires province, Argentina
  • Citing Article
  • April 2019

... Recently, Orallo et al. [136,137] have performed studies on the MAAs shinorine, porphyra-334 and shinorine dimethyl ester in micellar solutions to elucidate the photochemistry and photophysics in a closer-to-real sunscreen formulation environment. They found that the fluorescence quantum yield and lifetime were increased in micelle solutions compared to aqueous solutions; however, the main deactivation process remained via non-radiative decay. ...

Photochemistry and Photophysics of Shinorine Dimethyl Ester
  • Citing Article
  • January 2018

Photochemistry and Photobiology

... [17][18][19][20][21][22][23][24][25][26] The combination of silica-based systems and GA has been promising for biomedical applications. [27][28][29][30][31][32] Dong and coworkers studied the use of GA for active packaging composite films by incorporating different GA-SiO2 nanoparticles into chitosan. They showed that the incorporation of the modified silica nanoparticles with the mean sizes of (112.7 ± 0.55 and 408.7 ± 3.20 nm) significantly increased the antioxidant activity of the composite films. ...

Two choices for the functionalization of silica nanoparticles with gallic acid: characterization of the nanomaterials and their antimicrobial activity against Paenibacillus larvae

Journal of Nanoparticle Research

... Its activity was also stable under cooling and freezing temperatures for up to one year. Fangio and Fritz (2013) also obtained similar results in a storage study of CBLC produced by B. subtilis. The notable feature of the heat resistance and long storage at low temperatures of this bacteriocin supports its potential use as a food preservative in different processes such as drying, pasteurization, freezing and refrigeration, without a reduction in its antimicrobial activity. ...

Preliminary characterization of a bacteriocin-like substance produced by a bacillus subtilis isolated from argentinean vegetable food
  • Citing Article
  • January 2013

Italian Journal of Food Science

... Our results indicated that supplementation of the SPS-BV was as effective as commercial nisin for controlling food spoilage bacteria in dried, seasoned and crushed squids during storage at room temperature. Similar results were reported by Fangio and Fritz (2014). Crude extract of a bacteriocin-like substance produced by a harmless strain of B. cereus P9 was administrated in food samples. ...

Potential use of a bacteriocin-like substance in meat and vegetable food biopreservation
  • Citing Article
  • January 2014

International Food Research Journal

... Honey could inhibit bacterial growth through its high concentration of sugars, its low pH, enzymatic generation of hydrogen peroxide (H2O2), or the presence of certain protein or AJMAP V9N1 2023 168 phytochemical compounds, such as aromatic acids and phenols (Fangio 2007;Hegazi et al. 2017;Padungton and Kaneene 2003). However, to keep its inhibitory properties, honey must be stored away from heat and light (Bogdanov and Blumer 2001). ...

Actividad antimicrobiana de mieles del sudeste de la provincia de Buenos Aires frente a Escherichia coli

Revista Argentina de Microbiología