Manami Shishikura's research while affiliated with Tottori University and other places
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Publications (2)
Entoloma clypeatum species complex (ECSC) forms ectomycorrhiza-like roots (EMLR) with host plant species of Rosaceae or Ulmaceae. The EMLR colonized with ECSC are characterized by a thick fungal mantle, absence of a Hartig net structure, and collapse of the apical meristem caused by hyphal invasion. Some researchers have suggested parasitism of ECS...
The Entoloma clypeatum species complex, known as “Harushimeji” in Japan, associates with Rosaceae and Ulmaceae plant species. In this study, we successfully isolated cultures of this fungal group via basidiospore isolation from tentative four Harushimeji species using modified Norkrans's C (MNC) medium and MNC medium containing n-butyric acid. Colo...
Citations
... There are some lineages that have been suggested to be nested within ECM lineages and for which the nutritional mode is not very clear. They can form ECM or ECM like structures (for example 45,46 ), but may be parasitic rather than mutualistic or they also form parasitic relations 45,47 . These lineages were not included here. ...
... For instance, the culture-based technique failed to detect several ascomycete genera such as Verrucocladosporium and Toxicocladosporium that are known to be slow-growing even on water-rich media including potato dextrose agar (PDA) and malt extract agar (MEA) 23,24 . The culture-based technique also failed to detect several basidiomycete genera, including Entoloma that is known to grow well only on water-rich media such as Murashige and Skoog medium and PDA 25,26 . Thus, the DG18 medium used in this study is also likely unsuitable for other basidiomycetes such as Graphiola, Sterigmatomyces, and Exobasidium that were detected only using the DNA-based technique. ...
