Makie Kokubun's research while affiliated with Tohoku University and other places

Publications (51)

Article
Full-text available
Soil salinity is a major constraint to sustainable crop production. Genetic improvements are needed for growing soybean in salinity-prone environments. Salt-tolerant soybean genotypes alleviate a reduction in photosynthesis and growth under saline conditions; however, the detailed mechanisms involved remain unclear. Here, we aimed to clarify how Na...
Chapter
Food is essential to human life, but there remain not a few people who cannot be accessible to adequate amount and quality of food, especially in some developing countries. Since a major portion of human diet comes from crop products, a continuous increase in crop production is required to feed the burgeoning population. Crop growth is strongly reg...
Chapter
Cutting-edge science and technology such as genome analysis is a useful technology in genomic characterization of living organisms, biological diversity analysis, breeding, and any other genome-related science. In this chapter, we tried to find how the cutting-edge science and technology was utilized in solving the global-scale problems in developi...
Book
This book presents field studies on crop production in developing countries such as Vietnam, Kenya, Namibia, Colombia, Afghanistan and Sudan. Further, it examines the achievements of SATREPS, a development assistance program sponsored by the Japanese government that promotes international joint research to address these global issues. In this conte...
Article
Full-text available
Saline soils hamper various physiological functions in soybean [Glycine max (L.) Merr.]. One example is the reduction in nitrogen (N) uptake capacity, a major dysfunction that limits soybean growth and yield under saline conditions. Previous studies have revealed that tolerance to salinity varies with cultivar; however, the cultivars used in these...
Article
Full-text available
We conducted hydroponic culture experiments to characterize root traits in a rice cultivar ‘Puluik Arang’ that has been identified in a previous study as a cultivar that is adaptable to unflooded conditions. Root morphological traits and the expression of 11 aquaporin genes in rice seedlings (cv. Puluik Arang and cv. Akitakomachi) subjected to osmo...
Article
Full-text available
This study aimed to characterize the physiological and morphological traits that are associated with adaptation to unflooded soil conditions in rice. Four indica rice cultivars (Puluik Arang, Badari Dhan, Shwe Nang Gyi, and Ratul), which were previously identified as highly or less adaptable to unflooded soil conditions, were grown under flooded an...
Article
The germination and emergence of soybean [Glycine max (L.) Merr.] can be adversely affected by the presence of excessive water as a result of continuous rainfall. The pre-germination flooding-tolerant cultivars can be tolerant to hypoxia in the flooding field. The objective of this study was to clarify the relationship between germination under hyp...
Article
Full-text available
Salinity stress causes various physiological dysfunctions in soybean (Glycine max (L.) Merr.). For example, reduced nitrogen (N) uptake due to salt-induced depression of nodule formation severely limits soybean growth and yield. Super-nodulating soybean genotypes were previously identified by their superior N2 fixation and photosynthesis. Here, we...
Article
Soybean (Glycine max (L.) Merr.) is vulnerable to waterlogging, which threatens soybean productivity in humid regions of Asia. Waterlogging can happen during any growth stage of soybean, but flooding stress before, at or after germination causes severe seed and seedling damage, resulting in substantial reduction of grain yield at maturity. Under wa...
Article
In order to enhance rice productivity under water-deficient conditions, it is essential to improve nitrogen (N) uptake ability or its use efficiency. The objectives of the present study were to examine the genotypic variation in N uptake ability and physiological N use efficiency (PNUE) among 70 rice cultivars, and to clarify its association with b...
Article
Full-text available
The application of synthetic cytokinin (6-benzylaminopurine, BA) to racemes of soybean genotype IX93-100 at 7 days after anthesis (DAA) enhanced pod-set percentage of the florets at the 5th position and above (numbered from the base on rachis). The endogenous cytokinin (trans-zeatin riboside) content of individual florets was measured at the 1, 3,...
Article
Full-text available
Genetic improvement in water uptake ability and/or water use efficiency (WUE) of rice cultivars is one option to enhance productivity under water-limited conditions. We examined the genotypic variation in biomass production among 70 rice cultivars (69 cultivars of NIAS global rice core collection and Azucena) under different soil moisture condition...
Article
Yield responsiveness to elevated CO2 concentration [CO2] was previously found to be greater when nitrogen (N) was supplied in adequate amounts; however, it remains unclear whether genotypic differences in N2-fixing capacity affect yield responsiveness in soybean. We tested the hypothesis that yield responsiveness to elevated [CO2] in soybean is gre...
Article
NERICAs (New Rice for Africa), new genotypes produced from the interspecific hybridization of Oryza sativa and O. glaberrima, have been bred in an attempt to improve the yield potential of rice cultivars adapted to resource-limited conditions in Africa. Identification of physiological traits responsible for their high-yielding potential under such...
Article
Full-text available
Our previous study revealed that upland cultivars of New Rice for Africa (NERICAs) exhibited superior biomass production and N uptake compared with selected Japanese cultivars under upland conditions. The objective of this study was to examine whether the N uptake ability of upland NERICAs is attributable to their transpiration and exudation rates....
Article
Full-text available
Elevated CO2 increases seed production more in plant species that form a symbiotic association with N2–fixing bacteria than in species without such association. We studied the mechanism of the increase of seed production with elevated CO2 using nodulated soybean (Glycine max cv. Enrei) and its non-nodulated isogenic line (cv. En1282). Increase in s...
Article
A new rice cultivar Akita 63 which has a relatively heavy 1000-grain weight (30 g) is expected to possess a high yield potential. To develop a suitable nitrogen (N) fertilization method for maximizing the yield potential of Akita 63, we investigated the effects of three patterns of N fertilization; early topdressing (ET) [basal dressing, topdressin...
Article
Full-text available
We tested the hypothesis that elevated CO 2 concentration [CO 2 ]-induced enhancement of biomass production of soybean is greater in a genotype that has a higher nitrogen (N) fixation capacity. Furthermore, we analyzed the interactive effects of N fertilization, temperature and [CO 2 ] on biomass production. Three genetically related genotypes: Enr...
Article
Full-text available
Scarcity of water and N fertilizer are major constraints to rice production, particularly in developing countries where rainfed upland condition dominates. Improvement of genetic adaptability to inadequate water and N fertilizer is one option to maintain productivity in these regions. NERICAs are expected to yield higher under low input conditions,...
Article
The relative amount of roots (dry weight ratio of root to shoot, or root dry weight per spikelet) or the physiological activity of roots is correlated with the cool-weather resistance in rice plants. The objective of this study is to elucidate the effects of the relative amount or physiological activity of roots on the number of pollen grains (NPG)...
Article
The supernodulating soybean (Glycine max (L.) Merr.) cultivar Kanto 100 was previously characterized by superior nitrogen (N) fixation and photosynthesis, and resulting in high yields. However, this cultivar seems to be susceptible to waterlogging during the vegetative growth stage, which frequently occurs in major soybean producing areas in East A...
Article
Flooding during germination inhibits the sprouting and emergence of soybean [Glycine max (L.) Merr.], reducing subsequent growth and yield. Previous studies revealed that cultivars tolerant to pre-germination flooding have a mechanism of reducing water absorption speed (WAS) during the initial stage of inundation; however, seed structures involved...
Article
The supernodulating soybean cultivar "Sakukei 4" is considered to be potentially high-yielding because of its superior capability of nitrogen fixation and photosynthesis. Despite these desirable traits, Sakukei 4 was found to exhibit inferior initial growth under conventional culture conditions. To explore the method of high-yielding cultivation of...
Article
The supernodulating soybean cultivar Sakukei 4 was previously characterized by its superior capabilities of nitrogen (N) fixation and photosynthesis, and was expected to be high yielding. Since the N absorption by Sakukei 4 is largely dependent on N fixation, it may be strongly affected by waterlogging during the vegetative stage, which occurs freq...
Article
Full-text available
Soybean plants differentiate abundant floral buds, but most of them fail to grow pods and abort during development. Many studies indicated promotive effects of exogenously applied cytokinin on pod setting, but the effects of auxin application on pod set are ambiguous. In this study, we examined the changes in the concentrations of endogenous auxin...
Article
根粒超着生ダイズ品種作系4号は, 窒素固定能と葉面当り光合成能が高いことから多収のポテンシャルを持つと期待される. 本品種の宮城県内における収量性を窒素の肥効パターンの影響の面から検討するため, 年次と場所の異なる3つの圃場試験(試験1~3)を行った. 供試品種は作系4号のほか, 親品種エンレイおよび根粒非着生系統En1282を用い, 肥料は速効性肥料(尿素)と緩効性肥料(LP-70あるいはLP-100)を用いた. 3種類の肥料効果を比較した試験1(2001年, 仙台)では, 作系4号とエンレイの収量はLP-100>LP-70≒尿素であったのに対し, En1282では尿素>LP-70>LP-100であった. 尿素とLP-100を用いた試験2(2002年, 仙台), 試験3(2002年, 鳴子...
Article
Soybean requires more nitrogen (N) than gramineous crops because it accumulates a large amount of N in seeds, and its photosynthetic rate per leaf N is low. The supernodulating genotype Sakukei 4 has a superior symbiotic N, fixation capability, and thereby is potentially high-yielding. In our previous study, Sakukei 4 was characterized by having a...
Article
Flooding during germination often inhibits the germination and emergence of soybean [Glycine max (L.) Merr.], but little is known about the mechanisms involved in the tolerance of soybean cultivars to the damage caused by the flooding. The objectives of this study were to characterize the germination responses of soybean cultivars to pre-germinatio...
Article
: A large proportion of soybean flowers abort during development. Pod set is significantly affected by the availability of assimilate and cytokinin in the flowers, but their synergistic effects on pod set remain unclear. The objective of this study was to examine whether pod set at specific nodes of a plant is enhanced by increasing the source-sink...
Article
The supernodulating soybean cultivar Sakukei 4 was previously characterized by its superior ability to maintain a high leaf nitrogen (N) content and high photosynthetic rate. Despite these desirable traits, the growth performance of Sakukei 4 was inferior to that of its normally nodulating parental cultivar, Enrei. The physiological basis for the u...
Article
A retrospective analysis of the physiological basis of genetic yield improvement should provide us a direction for future yield improvement. The objectives of the present study were to evaluate the change in yield in leading rice cultivars that were bred and grown in the Tohoku region in the 20th century, and to find whether apparent photosynthetic...
Article
The supernodulating soybean genotype Sakukei 4 is potentially high-yielding. We characterized its leaf nitrogen (N) content, photosynthesis and growth at different developmental stages and under different dosages and types of N fertilizer, and compared it with its parental cultivar Enrei and the non-nodulating line En1282. At the pod-expansion and...
Article
Most of the supernodulating soybean lines were agronomically inferior to conventional genotypes. Our previous tests with ‘En6500’, a supernodulating mutant derived from a variety ‘Enrei’, revealed that its low growth and yield were not necessarily due to supernodulation. In our attempts to improve the yield of En6500 through crossing with Enrei, we...
Article
The effects of gibberellin, cytokinin and an inhibitor of gibberellin biosynthesis on spikelet fertility and the number of pollen grains were examined in rice plants which were cooled at the young microspore stage. Spikelet fertility in the cooled plants was decreased by the application of GA3 or trans-zeatin during the period from the primary rach...
Article
Many studies have confirmed that exogenous cytokinins increase tillering in grasses as wheat, oat and barley. In this study, the effects of benzylaminopurine (BA) on tillering of two rice cultivars, North Rose with low tillering ability and Sasanishiki with high tillering ability, were examined using hydroponics. Foliar spray of BA was applied to b...
Article
Full-text available
A large proportion of flowers abscise during development in soybean(Glycine max L.Merr.).A reduction in this abscission might increase pod and seed number, and thereby can lead to an increased yield.Previous studies showed that pod-set probability was greater at proximal positions of individual recemes, and that the probability was enhanced by the...
Article
A supernodulating and nitrate-tolerant soybean (Glycine max (L.) Merr.) mutant En6500 and its wild-type parent, cv. Enrei were cultured hydroponically at various nitrate (KNO3) levels (0.5, 5.0, and 15.0 mol m) to analyze the characteristics of nitrogen fixation, nitrate absorption, and associated characters in this mutant. The mutant produced a la...
Article
Nodule-like structures on the roots of seedlings of oilseed rape (Brassica campestris L. subsp. napus) cv. CO were induced by Bradyrhizobium sp. strain CP283 or A2P5 in the presence of Erwinia carotovora MAFF-301393, a bacterium that produces the cell wall-degrading enzymes cellulase and pectinase. E. carotovora was most effective at an inoculation...
Article
The characteristics of pod set and seed growth as affected by raceme order were investigated to determine the yield-determining process of soybean. Observations of racemes were made on the 4th, 7th, 10th, 13th and terminal nodes of the main stem of three cultivars (Indeterminate type : Harosoy ; Determinate types : Enrei, Tamahomare). Pod-setting r...
Article
Nodule formation in legume crops is a multistep process which involves the interactive gene expression of bacteria and host plants. Attempts to widen the genetic variation of symbiotic bacteria and host plants have led to the isolation of several mutants with a diverse capability of nodulation. The most striking variants in hosts are the supernodul...
Article
Based on grafting studies, both supernodulating (Carroll et al. 1985a, b) and hypernodulating (Gremaud and Harper 1989) soybean ([itGlycine max} L. Merr.) phenotypes were reported to be under the control of shoot factors (Delves et al. 1986, 1987; Cho and Harper 1991). Recently Akao and Kouchi (1992) have isolated a new supernodulating mutant (En65...
Article
The supernodulating and nitrate-tolerant soybean (Glycine max [L.] Merr.) mutant «En6500 and its parent cv. «Enrei were compared in their response to combined irradiance and nitrate treatments. The mutant exhibited substantially less overall dry matter (DM) production but had markedly more nodules plant-1, larger nodule DM, and higher acetylene red...
Article
The effects of exogenous addition of 2.26 μM 2,4-dichlorophenoxyacetate (2,4-D) at the time of sowing and 3 d after on nodulation and nodule function were investigated in soybean [Glycine max (L.) Merr.] cvs. “Enrei,” “Lee,” and “Nanbushirome.” Both treatments especially that at the time of sowing considerably depressed shoot and root development....
Article
In the typical climate of East Asia, summer crops develop large shoots with relatively poor root systems during the rainy season, leading to restricted photosynthetic activity in the following summer months. The objectives of the present study were 1) to determinate if there are specific differences in the photosynthetic responses of four legume cr...

Citations

... NaHS irrigation may have acted on root, inhibiting Cl absorption from soil to root and Cl transport from root to shoot. It is well known that regulation of Cl to shoot is important for salt tolerance of soybean (Valencia et al. 2008, Onodera et al. 2019, Fujii and Higuchi 2019. ...
... A cet effet, plusieurs méthodes de lutte (mécanique, physique, chimique et culturale) sont testées sans succès (Olivier, 1995b(Olivier, , 1996Showemimo et al., 2002 ;Boussim et al., 2012), avec des insuffisances d'ordre économique, sociale et environnementale (Olupot et al., 2003 ;Zia and Khan, 2004). La lutte biologique s'aborde de nos jours sous plusieurs aspects, de l'utilisation des microorganismes (bactéries, champignons, virus) jusqu'à l'utilisation des organismes (plante piège ou faux hôte) (Stoop et al., 1981 ;Waters et al., 2017 ;Kokubun, 2018). La lutte biologique contre Striga hermonthica occupe une place importante en malherbologie dans la gestion intégrée des plantes parasites (Rath et al., 2006 ;Lawane et al., 2009). ...
... Legume plants have been a victim of the adverse effects of salinity, such as slow growth, less water availability for uptake, and declined photosynthesis (Nadeem et al., 2019). Limitation of growth due to excessive salinity has been observed in many legumes including soybean (Song et al., 2017). Several genes of the NAC transcriptional factors family are involved in salt stress responses in plants (Li et al., 2021b). ...
... The cultivar, Puluik Arang showed increased water uptake maintained proper lateral root development compare to Akitakomachi. In Puluik Arang, the expression of OsTIP2;1 was higher and its protein mainly accumulated in endodermal cells compared to Akitakomachi (Matsunami et al., 2016). These results indicate that the fine-tuned expression of PIP and TIP regulate lateral root development possibly playing role in drought tolerance behavior observed in few plant species. ...
... Nature rice plants become likely to develop a variety of climatic conditions, including sustained aerobic, drought (radical or irregular) 47 , poor soil quality, and floods 35 . Hence, the classification among root epigenetic regulation characteristics desirable for versatility to the unique number of conditions encountered by rice grains, and even the hereditary territories accountable for such plasticity characteristics, can encourage the choice for large tolerance of rice genotypes to variable conditions to maintain reliable output 36 . ...
... Sakata et al. (2014) reported that low temperature reduced the endogenous levels of bioactive gibberellins in developing anthers, resulting in fewer pollen grains and lower spikelet fertility. In contrast, Zhang et al. (2001) found that the number of pollen grains was decreased by the application of GA 3 and increased by the application of an inhibitor of gibberellin biosynthesis, although they did not measure the level of gibberellins in the developing anthers. Based on level of gibberellins in the developing anther and stem, some QTLs for cold tolerance might affect both spikelet fertility and culm length. ...
... A positive linear relationship existed between S and total pod number in all cultivars (Fig. 2). Pod number is one of the important soybean-yield determining factors (Bernard and Harville, 1993;Board and Tan, 1995;Mathew et al., 2000;Kokubun, 2001;Kakiuchi and Kobata, 2004). The pod number per plant was highly correlated with W in all cultivars (Fig. 3). ...
... However, seeds may fail to germinate when there is an absent or small amount of oxygen entering the seed embryo through the micropyle under anoxia and/or hypoxia [12]. Anoxia is a condition when oxygen is unavailable (0 kPa oxygen) and hypoxia is a condition when oxygen is limited (<20.6 kPa oxygen) for seeds to germinate [12,13]. ...
... An increase in the occurrence of extreme weather events is expected due to variation in average rainfall and storms, resulting in drought and waterlogging of the soil in several regions of the world (LORETI et al., 2016). Soil waterlogging leads to considerable losses in agricultural production (PEDO et al., 2015;RHINE et al., 2010), especially in soils with high water tables, compacted soils with poor drainage (BATAGLIA; MASCARENHAS, 1981;KOKUBUN, 2013), and in lowland soils (PEDÓ et al., 2015). ...
... They were not markedly different from agronomic gain in yield observed with improved agronomic practices in the Sahel (Ibrahim et al., 2021) and across SSA countries . Higher agronomic gain in irrigated rice than rainfed lowland rice in this study is in line with previous studies in Asia and Africa (Matsunami et al., 2009;Vandamme et al., 2016). VHT consistently had a higher agronomic gain in yield than VC regardless of production system, season, agronomic practices, and types of controls. ...