M.V. Milenina’s research while affiliated with All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K.I. Skryabin and other places

The purpose of the research: to conduct commission and field testing of efficacy of the supramolecular complex of triclabendazole «Triclafascid» applied at the dose of 2,5-3,0 mg/kg against cattle fasciolosis. Materials and methods. Commission and field testing of Triclafascid were carried out in the private sector of the Chechen Republic in March-April 2017 on cattle naturally infected with Fasciola. Commission testing was performed on 30 animals infected with Fasciola that were divided into two equal groups of 15 animals each. Animals from the first group received triclafascid orally at the single therapeutic dose 2,5 mg a.i./kg (therapeutic dose 25 mg/kg) in water solution. The second group of animals as well as the first group received the substance of triclabendazole at five times lower dose (2,5 mg/kg) in water suspension. In field experiment, triclafascid was given individually to 108 animals at the dose of 3,0 mg a.i./kg (therapeutic dose 30 mg/kg) with compound feed. To determine the grade of animals’ invasion with fascioles, fecal samples were examined by Fuelleborn's method using ammonium nitrate. The average number of Fasciola eggs in 1 g of feces was defined with the use of VIGIS counting chamber. Fecal samples were investigated; the efficacy of the preparation was evaluated 30 days after dehelmintization. The efficacy of triclafascid was estimated by a «critical test» according to the Manual approved by World Association for the Advancement of Veterinary Parasitology (1995). Results and discussion. In commission testing of triclafascid for the treatment of cattle fasciolosis individually orally in water solution at the dose of 2,5 mg /kg and in field experiment at the dose of 3,0 mg /kg with feed compound at five times lower dose, 100 % efficacy was reached (in comparison with triclabendazole).

Out of 10 supramolecular triclabendazole complexes with various polymeric excipients (hydroxyethyl starch, polyvinyl pyrrolidone, pharmacopoeian arabinogalactan, technical, sodium carboxymethyl cellulose, silicon dioxide -SiO2, kelp, fucus), on the basis of toxicological and helminthological research and economic prospects for introduction into veterinary practice, a triclabendazole – arabinogalactan (technical grade) complex (triclofascide) was selected. This is an arabinogalactan (technical grade) polysaccharide-based complex of triclabendazole extracted from Siberian larch in the ratio 1:10. Triclofascide, in contrast to the substance of triclabendazole, is soluble in water, more easy to use and has a high anthelmintic efficiency in a 5 times smaller therapeutic dose. This reduces the cost of animal treatment in comparison to the treatment with triclabendazole substance. The drug has a high biological availability to the parasite. In the animal’s organism the drug dissociates into metabolites and having been excreted in faeces and urine in miniscule amounts. Triclofascide preclinical toxicology trial showed that in the parameters of acute toxicity, acute cutaneous toxicity at enteral routes of administration to white mice and rats it may be classifie as IV hazard class. It has no adverse irritating effect on intact skin, and belongs to a group of substances with slightly pronounced cumulative properties. It does not show immunotoxic, embryotoxic and teratogenic activity. Triclofascide used for treatment of an acute and chronic fascioliasis: for sheep in a therapeutic dose of 2.0 mg/kg individually in the form of an aqueous solution; by group method mixed with food in a therapeutic dose of 3.0 mg/kg; for cattle, respectively, 2.5 and 3.0 mg/kg.