M.R. Badhe's research while affiliated with Central Institute of Freshwater Aquaculture and other places
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Publications (7)
Aim
An immunoproteomic approach was followed to identify immunoreactive antigens of fish ectoparasite, Argulus siamensis with rohu (Labeo rohita) immune sera for screening of potential vaccine candidates.
Materials and results
The whole adult Argulus antigen was run in 2D electrophoresis with IEF in 7 cm IPG strips of pH 4‐7 and SDS‐PAGE with 12%...
Lectins are proteins that bind to the carbohydrate moieties on surface of bacteria, erythrocytes and other cells of invertebrates causing agglutination and mediate in recognition of foreign substances. In the present study, we isolated and characterized a lectin molecule present in the hemolymph of Macrobrachium rosenbergii, an important cultured f...
A study was undertaken to develop a western blot method for detection of immunogenic proteins of fish ectoparasite, Argulus siamensis for its further use as potential vaccine candidates. Argulus antigens were prepared by homogenization and injected to rohu (Labeo rohita) juveniles for development of immune serum. The serum was used to immunostain t...
Citations
... The ICAR-CIFA has interdisciplinary research collaborations, proper amenities for wet lab maintenance, skilled manpower, basic instruments, and molecular biology laboratory facility with widely experienced researchers to address the challenges faced in fish louse Argulus management. Currently, the group is actively involved in screening as well as delivery of vaccine targets by a combination of genomics and immunoproteomics [35] approach through exploration of mucosal immunity in fish. ...
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... Indirect methods were used to obtain NO level in the serum by estimating nitrite/nitrate using Griess reaction as per the methods described by Das et al. (2018). To estimate nitrite level in the serum, about 100 µl of serum was diluted with 400 µl of carbonate buffer. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272171771625490-1441902215972_Q64/Subas-Parija.jpg)
... Over the years, ICAR-CIFA has developed a challenge facility for year-round availability of Argulus parasite and standardized challenge protocol. In another attempt, a western blot method has also been developed and used to identify immunogenic proteins of Argulus that could be targeted as potential vaccine candidates [34]. The ICAR-CIFA has interdisciplinary research collaborations, proper amenities for wet lab maintenance, skilled manpower, basic instruments, and molecular biology laboratory facility with widely experienced researchers to address the challenges faced in fish louse Argulus management. ...
