M.N. Somchit's research while affiliated with Universiti Teknologi MARA and other places

Publications (94)

Article
Full-text available
Garcinia hombroniana has been used in Malay traditional medicine to treat various disorders such as abdominal pain and gonorrhea, and little is known about its toxicological properties. This study investigated the acute toxicological effects of the plant's leaves aqueous extract using theoral acute toxic class (ATC) method. Twenty-four female Sprag...
Article
Full-text available
Background Histamine is established as a potent inflammatory mediator and it is known to increased endothelial permeability by promoting gap formation between endothelial cells. Previous studies have shown that aqueous extract of Bixa orellana leaves (AEBO) exhibits antihistamine activity in vivo, yet the mechanism of its action on endothelial barr...
Article
Muntingia calabura L. have been used in Southeast Asia and tropical America as antipyretic, antiseptic, analgesic, antispasmodic and liver tonic. This study aims to determine the acute toxicity and the metabolic pathways involved in the hepatoprotective mechanism of M. calabura. CCl4-induced hepatotoxic rat model was developed and a dose dependent...
Article
Full-text available
The anti-Trypanosoma evansi activity of Garcinia hombroniana (seashore mangosteen) leaves aqueous extract was tested on experimentally infected Sprague-Dawley rats. Treatment of infected rats with G. hombroniana extract resulted in a significantly extended post-infection longevity (p < 0.05), compared to the untreated control group. The possible mo...
Article
Full-text available
Trypanosoma evansi, the causative agent of "surra", infects many species of wild and domestic animals worldwide. In the current study, the aqueous and ethanolic extracts of six medicinal plants, namely, Aquilaria malaccensis, Derris elliptica, Garcinia hombroniana, Goniothalamus umbrosus, Nigella sativa, and Strobilanthes crispus were screened in v...
Article
Extracts of three different plant species; Curcuma longa L., Centella asiatica L. and Strobilanthes crispus L. which are used widely in Malaysian traditional medicine are investigated for antiviral activity against alpha-herpesvirus (pseudorabies virus). The methanol extract (ME) and aqueous extract (AE) were tested in three cell lines; African Gre...
Article
Water extracts of Carica papaya and Ananas comosus extracts were administered orally at doses of 5 and 10 mg/kg to Spraque Dawley rats. Two other groups were given commercial diuretics, furosemide and hydrochlorthiazide at 10 mg/kg. Urine volume, urine pH, urine density and urine electrolytes were determined every hour for 4 hours. Blood was taken...
Conference Paper
Full-text available
Trypanosomes are protozoan parasites living in the blood of vertebrates and surra, which is caused by Trypanosoma evansi, causes often a debilitating disease in many species of domesticated and wildlife animals. The few drugs currently available for chemotherapy of trypanosomiasis are old, toxic and there are reports showing the emergence of resist...
Article
Full-text available
In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n = 6) were used and administered orally once daily with 10% DMSO (negative...
Article
Full-text available
An experiment was conducted to determine the effects of combining both pleasant and unpleasant contacts with human beings on physiology and behavior of broiler chickens. Birds were subjected to the following treatments: (i) received no physical or visual contact with humans (control); (ii) from d 1 to 28, chicks were individually stroked gently for...
Conference Paper
Full-text available
The aqueous and ethanolic extracts from five medicinal plants namely Acanthus ilicifolius, Aquilaria malaccensis, Goniothalamus tapis, Pereskia grandifolia, and Strobilanthes crispus, were tested in vitro against Trypanosoma evansi, a hemoprotozoan parasite causing surra in a vast variety of domesticated and wild animals. The antitrypanosomal activ...
Article
Full-text available
The present study was conducted to assess the effects of dietary supplementation of Zingiber officinale and Zingiber zerumbet and to heat-stressed broiler chickens on heat shock protein (HSP) 70 density, plasma corticosterone concentration (CORT), heterophil to lymphocyte ratio (HLR) and body temperature. Beginning from day 28, chicks were divided...
Article
To determine the potential antiulcer activity of methanol extract of Melastoma malabathricum leaves (MEMM) using various established rat models. Ten groups of rats were used and orally administered 10% DMSO (negative control), 100 mg/kg ranitidine (positive control) or MEMM (50, 250 and 500 mg/kg) followed by gastric ulcer induction either using et...
Article
Much attention has been given to unsaturated carbon 18 fatty acids in milk, particularly conjugated linoleic acids (CLAs) which have a beneficial effect on human health. This study was undertaken to investigate the effect of breed on cis-9, trans-11 and trans-10, cis-12 CLA isomers in the milk fat of dairy cattle and their relationship with other u...
Article
This experiment was conducted to investigate the effects of supplementing various dosages of Euphorbia hirta powderon performance, digestibility, and ileal microflora populationin broiler chickens. E. hirta was supplemented at inclusion rates of 2.5 (EH2.5), 5.0 (EH5.0) and 7.5 (EH7.5) g/kg from d 1 to 42 and compared with an antibiotic group (0.02...
Article
Full-text available
Zerumbone a monocyclic sisquiterpene, is the major constituent of Zingiber zerumbet Smith. In Southeast Asia, Z. zerumbet is being used for its anti-inflammatory and analgesic properties. The objective of this current investigation was to evaluate the in vivo anti-inflammatory and analgesic activities of zerumbone isolated from Z. zerumbet in rats....
Article
Full-text available
Drymoglossum piloselloides (DP) is a small epiphytic fern commonly seen on trunks of older trees. The antimicrobial activity of water, ethanol and chloroform extracts of DP was tested against Trichophyton rubrum ATCC 40051 and Trichophyton mentagrophytes ATCC 40004 which are the two most commonly cause of Athlete's foot, Candida albicans ATCC 14053...
Article
Bauhinia purpurea (Fabaceae) is a medicinal plant traditionally used to treat various ailments, including ulcers. In order to establish pharmacological properties of the leaf of Bauhinia purpurea, studies were performed on antiulcer activity of the plant's aqueous extract. The Bauhinia purpurea aqueous extract (BPAE) was prepared in the doses of 10...
Article
Full-text available
The body normally hosts a variety of saprotrophic micro-organisms that may cause infection. Athlete' s foot causes scaling, flaking and itching of the affected skin. Blisters and cracked skin may also occur, leading to exposed raw tissue, pain, swelling and inflammation. Secondary bacterial infection can accompany the fungal infection. This work ex...
Article
The effects of edible film from modified sago starch were determined on female Sprague-Dawley rats where the aim was to develop a basic guideline for safe use of edible films as food packaging. Rats were fed with 2 g kg<SUP>-1</SUP> (low dose) and 5 g kg<SUP>-1</SUP> (high dose) of body weight edible film for 28 days and the control rats only recei...
Conference Paper
Full-text available
Trypanosoma evansi is a protozoan parasite causing a disease in human and animals known as “surra”. Current chemotherapeutic options for trypanosomiasis in humans and livestock are very limited. In the present study, the antitrypanosomal effect of the aqueous, ethanolic and chloroform extracts from the bulks of garlic (Allium sativum) and seeds of...
Article
The present study was carried out to investigate the antinociceptive and anti-inflammatory activities of virgin coconut oil (VCO) produced by the Malaysian Agriculture Research and Development Institute (MARDI) using various in vivo models. Two types of VCOs, produced via standard drying (VCOA) and fermentation (VCOB) processes were used in this st...
Article
Full-text available
The present study was conducted to assess the anti-inflammatory effect of a crude aqueous extract of Bixa orellana leaves (AEBO) and to examine the possible involvement of nitric oxide (NO) in its anti-inflammatory mechanism. The air-dried, powdered leaves were soaked in distilled water (1:20 w/v) at 50°C for 24 h and the supernatant obtained was f...
Article
Full-text available
The in vitro cytotoxic and antioxidant properties of the aqueous, chloroform and methanol extracts of the Dicranopteris linearis leaves were investigated in the present study. The cytotoxic effect was determined against the normal (3T3) and cancer cells' lines (MCF-7, HeLa, HT-29, HL-60, K-562 and MDA-MB-231) using the 3,(4,5-dimethylthiazol-2-yl)-...
Article
Full-text available
The antiproliferative and antioxidant activities of various extracts of the leaves of Bauhinia purpurea were studied using in vitro standard assays. The aqueous and chloroform extracts successfully inhibited the proliferation of all cancer cells while the methanol extract inhibited the proliferation of all cells except the CEMss cells when assessed...
Article
Full-text available
Conjugated Linoleic Acids (CLAs) are group of positional and geometric isomers of octadecadienoic acid with conjugated double bonds and believed to have many health promoting effects. The present study focused on the quantitative analysis of CLAs in liver, Superficial Pectoral (SP), Longissimus Dorsi (LD) and Semimembranosus (SM) muscles of local M...
Article
Full-text available
The present study aims to determine the hepatoprotective effect of MARDI-produced virgin coconut oils, prepared by dried- or fermented-processed methods, using the paracetamol-induced liver damage in rats. Liver injury induced by 3 g/kg paracetamol increased the liver weight per 100 g bodyweight indicating liver damage. Histological observation als...
Article
The antimicrobial activity of water, ethanol and chloroform extracts of Acalypha indica was tested against four bacterial and fungal strains using the disc diffusion method. The antibacterial activity against gram positive bacteria was more pronounced (p < 0.05) in water and ethanol extracts. Antifungal activity was more significant (p < 0.05) only...
Article
Full-text available
Virgin coconut oil (VCO), or in Malays known as 'minyak kelapa dara', has gain a lot of attention recently due to various medicinal values. The aim of the present study was to evaluate the effect of feeding two types of VCOs, VCOA (produced via a standard drying method) or VCOB (prepared via fermentation process), that were produced by Malaysia Agr...
Conference Paper
Full-text available
Much attention has been given to the milk unsaturated carbon 18 fatty acids in particular conjugated linoleic acid (CLA)that have beneficial effects for human health. This study was undertaken to determine the deposition of cis-9, trans-11 and trans-10, cis-12 CLA isomers in milk fat ofMafriwal and Jersey cows.All the animalswere grazed on pasture...
Article
Full-text available
The aim of the present study is to determine the in vitro antimicrobial activity of various extracts, partitions and fractions of Muntingia calabura (Elaeocarpaceae) leaves against a selected panel of microorganisms. The leaves of M. calabura were soaked separately in the aqueous, chloroform and methanol solvent systems in the ratio of 1:20 (w/v) f...
Conference Paper
Full-text available
Conjugated linoleic acids (CLAs) are a mixture of positional and geometric isomers of linoleic acid (LA) containing conjugated double bonds. It have been well known as having many diverse biological activities, including anti-carcinogenic, antioxidant, anti-atherosclerotic, anti-obesity, anti-diabetic and immune system enhancement. Therefore, this...
Conference Paper
Full-text available
Diet has a major influence on milk fatty acid composition; however, effects of physiological factors have received little attention. Therefore, this study was undertaken to investigate the milk fat contents of fatty acids profile of Mafriwal and Jersey cows under same feeding system. All the cows were grazed on pasture and given 5.5 kg of concentra...
Article
Canine dirofilariasis is a common tropical parasitic disease of companion animals, caused by infestation of Dirofilaria immitis filarids within the pulmonary arteries and extending into the right heart. Increased reports of adverse reactions elicited by current microfilaricidal agents against D. immitis such as neurological disorders, circulatory c...
Article
This study was focused on the quantitative analysis of Conjugated Linoleic Acids (CLAs) in rumen liquor and assessment of the relationship between CLAs and other carbon 18 fatty acids and rumen pH of cattle slaughtered for meat consumption. The fatty acids were extracted using modified Folch method and determined by gas chromatography. The mean (n...
Article
Orthosiphon stamineus has been used in traditional medicine for centuries especially to treat diseases of the urinary system. To investigate the diuretic activity, to elucidate its possible mechanism and to evaluate the renal effects of Orthosiphon stamineus extract. Water extracts were administered orally at doses of 5 and 10 mg/kg to Sprague-Dawl...
Article
Full-text available
A critical review of the levels of persistence organic chemicals in Malaysian waters from 1980 to 2002. This review concentrated on the detection several toxic substances by groups of scientists in the marine environment, evaluated their quantities and provides estimates of their potential danger for the health of both marine life and humans. The c...
Article
The present study was carried out to establish the antinociceptive, anti-inflammatory and antipyretic properties of an aqueous extract of jute plant leaves, Corchorus capsularis L. (Tiliaceae), in experimental animals. The antinociceptive activity was measured using the abdominal constriction, hot plate and formalin tests, while the anti-inflammato...
Article
Moringa oleifera L. (Moringaceae) is known to possess high nutritional value and is used in a folklore medicine to treat various ailments related to pain and inflammation. The aim of the present study was to evaluate the antinociceptive and anti-inflammatory effects of the aqueous extract of the leaves of M. oleifera in laboratory animals, using th...
Article
Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) are heterogenous group of compounds used to cure and prevent inflammation. It was demonstrated that NSAIDs has the ability to inhibit the viability of colon cancer cells in vitro. We investigated the effects of Piroxicam and mefenamic acid on the viability of 4 cancer cell lines in which 2 of them are c...
Article
Full-text available
The current study was carried out to investigate the possible involvement of the L-arginine/nitric oxide/cyclic guanosine monophosphate (L-arginine/NO/cGMP) pathway in the aqueous extract of Corchorus olitorius. L. (Tiliaceae) (AECO) antinociception in mice, assessed by the abdominal constriction test. The AECO, obtained by soaking the dried leaves...
Article
The aqueous extract of Ficus deltoidea leaves was evaluated for possible antinociceptive activity in three models of nociception, namely, acetic acid-induced abdominal writhing, formalin and hot plate test. The results of the present study showed that intraperitoneal administration of the F. deltoidea leaves aqueous extract at the dose of 1, 50 and...
Article
Full-text available
The aim of this study was to compare selected sterilization methods to maintain high susceptibility of antibacterial activities of aqueous extracts of herbal plants. Autoclave-sterilized Impregnated disk and Impregnated disk sterilized by Acrodisc syringe filter were embedded on Mueller-Hinton Agar (MHA) plates seeded with the respective test micro...
Article
The present study was carried out to elucidate the antinociceptive, antiinflammatory and antipyretic properties of the aqueous and lipid-based extracts of Channa striatus fillet in rats. The antinociceptive activity was assessed using the formalin test, and the antiinflammatory and antipyretic activities were assessed using the carrageenan-induced...
Article
Full-text available
In order to collect ethnobotanical information about growth and health promoter plants as feed additive in broiler chickens, five medicinal plants Euphorbia hirta , Solanum torvum , Zingiber officinale , Curcuma longa and Zingiber zerumbet used by traditional medical practitioners for the treatment of several ailments of microbial and non-microbial...
Article
The present study was carried out to determine the involvement of various receptor antagonists in and the effect of pH and enzymes on the recently reported antinociceptive activity of aqueous extract of Muntingia calabura leaves (MCAE) using the abdominal constriction test. The extract was prepared by soaking the dried powdered leaves of M. calabur...
Article
The present study was carried out to establish the antinociceptive and anti-inflammatory activities of the aqueous extract of Lonicera japonica flowers' buds in various experimental animal models. The antinociceptive activity was measured using the abdominal construction, hot plate and formalin tests, while, the anti-inflammatory was measured using...
Article
The present study was conducted to evaluate the analgesic activity of ethanolic extract of Andrographis paniculata (AP) in mice. The analgesic investigations were carried out using the acetic acid-induced abdominal writhing and the hot-plate tests. It was demonstrated that intraperitoneal (i.p.) administration of the extract at a dose of 30, 100, 3...
Article
1. The present study was performed in order to determine the amino acid and fatty acid composition of an aqueous extract of the freshwater fish Channa striatus, obtained by soaking (1:2, w/v) fresh fillets overnight in a chloroform:methanol (2:1, v/v) solvent, to elucidate the mechanism responsible for its antinociceptive activity and to clarify th...
Article
Objective: The effects of gastroprotective properties of Spirulina platensis was investigated in acetic acid and ethanol induced ulcers in rats. Methods: Administration of 2 and 4mg/kg Spirulina platensis extract for 7 days. After day 7, oral administration of either 80% (v/v) ethanol or 6% (v/v) acetic acid. Control rats received saline or anti-ul...
Article
Full-text available
The present study was carried out to establish the antinociceptive, anti-inflammatory, and antipyretic properties of the aqueous extract of Melastoma malabathricum leaves in experimental animals. The antinociceptive activity was measured using abdominal constriction, hot-plate, and formalin tests, whereas the anti-inflammatory and antipyretic activ...
Article
Full-text available
The objective of this current study was to analyze the biochemical compositions of three Malaysian Channa spp. fish. The proximate analysis revealed that the protein content of Channa lucius, Channa micropeltes and Channa striatus was 19.9%, 22.1%, 23.0% (% of dry weight), respectively. The total lipid content was generally high, ranging from 5.7%...
Article
Many plant-derived natural compounds have been reported previously to inhibit the production of important pro-inflammatory mediators such as nitric oxide, prostaglandin E2, TNF-alpha and reactive oxygen species by suppressing inducible enzyme expression via inhibition of the mitogen-activated protein kinase pathway and nuclear translocation of crit...
Article
Full-text available
The present study was carried out to evaluate the potential of C. nucifera as antipyretic, anti-inflammatory and wound healing agents. The fresh juice of C. nucifera (FJCN) was directly used while its aqueous kernel extract (AKCN) was obtained after 72 h soaking of 1:2 (w/v) fresh kernel in 2:1 (v/v) chloroform:methanol. The extracts, in the concen...
Article
Objectives: Aim of this study is to investigate the effects of Neem (Azadirachta indica) leaf extract on serum lipid parameters and C-reactive protein (CRP) concentrations. The association between CRP and serum lipid profiles in hypercholesterolemic rats is also investigated. Methods: Total cholesterol (TC), low density lipoprotein (LDL), high de n...
Article
The present study was carried out to investigate on the possible antibacterial activity of aqueous (AEMC), methanol (MEMC) and chloroform (CEMC) extracts of Muntingia calabura using the in vitro disc diffusion methods. The sterilized blank discs (6 mm diameter) was impregnated with 20 μL of the respective extract (in the concentration of 10,000, 40...
Article
The present study was carried out to elucidate some of the basic physical properties of the bioactive compounds responsible for Channa striatus antinociception. The aqueous extract of C. striatus was obtained after soaking the fish fresh fillet in the ratio of 1:2 (w/v) in chloroform: methanol (2:1; v:v) overnight. The extract, in the concentration...
Article
The present study was carried out to evaluate the potential of aqueous (AETT), ethanol (EETT) and chloroform (CETT) extracts of Tinosprora crispa as antibacterial agent against selected Gram positive ( Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Stretococcus pneumoniae and Clostridium diphtheriae ) and Gram negative ( Shigella f...
Article
Full-text available
The presence study was carried out to investigate on the possible antibacterial activity of methanol (MECO) and chloroform (CECO) extracts of Corchorus olitorius (Senaung betina) using the in vitro disc diffusion methods. The sterilized blank discs (6 mm diameter) was impregnated with 20 μL of the respective extract (in the concentration of 10,000;...
Article
Itraconazole and fluconazole are potent wide spectrum antifungal drugs. Both of these drugs induce hepatotoxicity clinically. The mechanism underlying the hepatotoxicity is unknown. The purpose of this study was to investigate the role of phenobarbital (PB), an inducer of cytochrome P450 (CYP), and SKF 525A, an inhibitor of CYP, in the mechanism of...
Article
Full-text available
To determine on the antibacterial activity of the leaves and acute toxicity level of the leaves, tender leaves and rhizomes of Manihot esculenta var. Sri Pontian extracts. The chloroform (CME1) and ethanol (EME1) leaves extracts of M. esculenta (25, 50 and 100% concentrations) were tested against a selected groups of Gram positive and Gram negative...
Article
In the present study, three Malaysian Channa spp. fish Channa striatus, Channa micropeltes and Channa lucius were assessed for their analgesic activity. Distilled water and mixture of chloroform: methanol were used for extraction. The analgesic or antinociceptive activity was investigated by abdominal writhing and hot plate test. The water extract...
Article
The aim of present study was to investigate the potential roles of aqueous extracts of A. paniculate in lowering the plasama lipid parameter which is responsible for hyperlipidemia and its damaging consequences and also to determine the kidney and liver functions of rats. Plasma Total Cholesterol (TC), Low Density Lipoproteins (LDL) cholesterol and...
Article
Full-text available
A series of preliminary studies was carried out to evaluate the antinociceptive (pain relief) activity of the aqueous extract of Corchorus olitorius L. leaves (COAE) and to determine the influence of temperature and opioid receptors on COAE activity using the abdominal constriction and hot plate tests in mice. COAE, at concentrations of 10, 25, 50,...
Article
The effects of an aqueous supernatant of haruan (ASH) (Channa striatus) fillet extract on various antinociception receptor system activities were examined using a mouse abdominal-constriction model. Mice that were pretreated with distilled water, s.c., followed 10 min later by administration of 25%, 50%, and 100% concentration ASH, s.c., produced a...
Article
Full-text available
The objective of the present study was to evaluate the anti-pyretic and analgesic activities of aqueous and ethanol extracts of Zingiber zerumbet rhizomes. The anti-pyretic activity of Zingiber zerumbet (25, 50 and 100 mg kg<SUP>-1</SUP>) was studied in Brewer's yeast-induced pyrexia in rats. The analgesic activity of Zingiber zerumbet (10, 25, 50...
Article
The onset and offset time of aqueous supernatant of haruan ( Channa striatus ) fillet (ASH) antinociception was evaluated using the abdominal constriction test. The ASH, extracted using chloroform:methanol (2:1; v:v) overnight and then evaporated to remove the residual solvent, was prepared in the concentration of 10, 25 and 100% and administered s...
Article
The hepatoprotective activity of the ethanol extract of Curcuma longa was investigated against paracetamol (acetaminophen, 4-hydroxy acetanilide)-induced liver damage in rats. Paracetamol at 600 mg kg<sup>-1</sup>, induced liver damage in rats manifested by statistically increased serum alanine aminotransferase (ALT), aspartate aminotransferase (AS...
Article
The antinociceptive effect of the ethanolic extract of Melastoma malabathricum (MME) was investigated using acetic acid-induced abdominal writhing test and hot-plate test in mice. It was demonstrated that the extract (30-300 mg/kg, i.p.) strongly and dose-dependently inhibited the acetic acid-induced writhing with an ED(50) of 100 (78-160) mg/kg i....
Article
Full-text available
Ethanol and aqueous extracts of Cassia auriculata were tested in vitro against fungi (Candida albicans and Microsporum canis) and bacteria (Escherichia coli, Salmonella enteriditis, Staphylococcus aureus and Bacillus subtilis). M. canis showed dose-dependent susceptibility only towards ethanol leaf and bark extracts. C. albicans, were resistant to...
Article
Full-text available
Non-steroidal anti-inflammatory drugs (NSAIDs) are used to treat musculoskeletal disorders, inflammation and to control pain. Virtually all NSAIDs are capable of producing liver injury ranging from mild reversible elevation of liver enzymes to severe hepatic necrosis. Mice were dosed intraperitoneally with mefenamic acid either one day at 100mg/kg...
Article
This study was attempted to preliminarily establish the basic chemical properties of the bioactive compound responsible for Channa striatus (Haruan) mucus extract antinociceptive activity using the acetic acid-induced abdominal constriction test in mice. Pre-treatment of extract with enzymes were carried out at the temperature of 40?C for 60 min. T...
Article
Itraconazole and fluconazole are oral antifungal drugs, which have a wide spectrum antifungal activity and better efficacy than the older drugs. However, both drugs have been associated with hepatotoxicity in susceptible patients. The mechanism of antifungal drug-induced hepatotoxicity is largely unknown. Therefore, the aim of this present study wa...