M.M.A. BRADFORD’s research while affiliated with University of Georgia and other places

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Publications (1)


A Rapid and Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding
  • Literature Review

May 1976

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2,081 Reads

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102,838 Citations

Analytical Biochemistry

M.M.A. BRADFORD

A protein determination method which involves the binding of Coomassie Brilliant Blue G-250 to protein is described. The binding of the dye to protein causes a shift in the absorption maximum of the dye from 465 to 595 nm, and it is the increase in absorption at 595 nm which is monitored. This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr. There is little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose. A small amount of color is developed in the presence of strongly alkaline buffering agents, but the assay may be run accurately by the use of proper buffer controls. The only components found to give excessive interfering color in the assay are relatively large amounts of detergents such as sodium dodecyl sulfate, Triton X-100, and commercial glassware detergents. Interference by small amounts of detergent may be eliminated by the use of proper controls.

Citations (1)


... There is a variety of analytical methods for controlling the quality of wine, but most of them need complex and expensive equipment and have a high degree of complexity to perform, so they cannot be applied by small businesses as well as in field and home conditions. The alternative approach considered in the following article is spectrophotometric determination of total protein content in wine, based on interaction between proteins and Coomassie G-250 dye, also called the Bradford method [7]. ...

Reference:

Total protein content in samples of dry red wine from various manufacturers for quality and authenticity control
A Rapid and Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding
  • Citing Article
  • May 1976

Analytical Biochemistry