M.M.A. BRADFORD's research while affiliated with University of Georgia and other places
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Publication (1)
A protein determination method which involves the binding of Coomassie Brilliant Blue G-250 to protein is described. The binding of the dye to protein causes a shift in the absorption maximum of the dye from 465 to 595 nm, and it is the increase in absorption at 595 nm which is monitored. This assay is very reproducible and rapid with the dye bindi...
Citations
... Although widely used, the Kjeldahl method, based on conversion of total nitrogen to ammonium by acid digestion, distillation of the product as ammonia, absorption on an acid solution, and titration of the absorbing solution, is time-consuming and generates significant amounts of waste [4]. Classical spectrophotometric methods, such as Lowry [6], Biuret [7], and Bradford [8] ones are usual, however, they are time consuming [9] and the responses not encompass a wide range of proteins. ...